* Restriction enzymes: * Have sticky ends or overhangs of DNA.
* This helps to glue in the gene of interest in the right
direction. * Must find a plasmid with the appropriate sticky ends
that match your gene.
* Gel Electrophoresis: *
http://www.dnalc.org/resources/animations/gel electrophoresis.html
http://www.dnalc.org/resources/animations/gel electrophoresis.html
* Gene Chip: * http://www.sumanasinc.com/webcontent/anima
tions/content/dnachips2.html
http://www.sumanasinc.com/webcontent/anima
tions/content/dnachips2.html * How could gene chip technology
transform medicine as we know it?
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Slide 10
* Learn how a mouse is cloned. *
http://learn.genetics.utah.edu/content/cloning/ clickandclone/
http://learn.genetics.utah.edu/content/cloning/ clickandclone/ *
Many copies of identical mice are cloned from mouse embryos. You
can have essentially 1000 identical twins with the same genetics. *
This has been used in science for many years.
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Slide 12
* Epigenetic patterns already in place (how is DNA tagged to
turn genes on and off?) * Transplantation process is costly. *
Cells are genetically older. Telomerase has been deactivated.