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- determine the presence of fecal coliform in the fishes using
the MPN method
- to compare the results they exceed to the standard MPN
count
- provide information
- may give indication that the fishes are not properlyhandled
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Scope
Test only the fishes which are sold by fish endors
Only the skin and tissue of the fish will be tested
Only fecal coliform bacteria will be tested and will
be determined
Limitations Getting and testing fish samples from other area
Testing the water quality
Monitoring fish temperature
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MATERIALS
ice box
alcohol lamp
inoculating loop scalpel
surgical glo es
blender
test tubes
petri plates
durham tubes
MEDIA
L-EMB Broth
Lactose Broth
BGLB Broth
Peptone Water
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Tissue AnalysisGetting fish samples
Culture of Bacteria(lactose broth medium)
Transfer to another media(BGLB medium)
Recording of results
Obser ation
Analysis of data
Transfer to another media(L-EMB medium)
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TISSUE ANALYSIS
1pait
2tilapia
COMPOSITES
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Sample 1 TRIAL 1 TRIAL 2 TRIAL 3
10 ml 1 + + +
2 + + +
3 + + +
1.0 ml 1 + + +2 + + +
3 + + +
0.1 ml 1 + + +
2 + + +
3 + + +
LACTOSE BROTH (Presumptive Results)Sample 2 TRIAL 1 TRIAL 2 TRIAL 3
10 ml 1 + + +
2 + + +
3 + + +
1.0 ml 1 + + +2 + + +
3 + + +
0.1 ml 1 + + +
2 + + +
3 + + +
ALL LACTOSE BROTH TUBES
WERE POSITIVE.
RESULTS & DISCUSSIONS
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BGLB (Coliform Confirmation)
Sample1 TRIAL1 TRIAL2 TRIAL3
10 ml 1 + + +
2 + + +
3 + + +
1.0 ml1 + + +2 + + +
3 + + +
0.1 ml1 + + +
2 + + +
3 + + +
Sample2 TRIAL1 TRIAL2 TRIAL3
10 ml 1 + + +
2 + + +
3 + + +
1.0 ml1 + + +2 + + +
3 + + +
0.1 ml1 + + +
2 - + -
3 + + +
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SAMPLING DILUTION TRIAL
1 2 3
1 10-1
10-2
10-3
Dark pink colonies
Pink colonies
Metallic sheen
Metallic sheen
Metallic sheen
Metallic sheen
Pink colonies
Metallic sheen
Pink colonies
Metallic sheen, pink colonies
Metallic sheen
Pink colonies
Pink colonies
Pink colonies
Metallic sheen
Pink colonies
Pink colonies
Pink colonies
Pink colonies
Pink colonies
Pink colonies
Pink colonies
Pink colonies
Pink colonies
Pink colonies
Pink colonies
Pink colonies
2 10-1
10-2
10-3
Metallic sheen
Pink colonies
Dark pink colonies
Metallic sheen
Metallic sheen
Metallic sheen
Metallic sheen
Metallic sheen
Pink colonies
Pink colonies
Pink colonies
Metallic sheen
Pink colonies
Metallic sheen
Metallic sheen
Metallic sheen
Pink colonies
Pink colonies
Pink colonies
Pink colonies
Pink colonies
Pink colonies
Pink colonies
Pink colonies
Pink colonies
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Fecal coliform populations in the fishes were
high reaching more than 1,100 MPN-100g
which were beyond than the recommended
safety levels and prescribed limits of(Bureau ofFood and
Drugs).
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Base on the results, fishes sold in
along the highway of Balo-i arecontaminated with fecal coliform.
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