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- determine the presence of fecal coliform in the fishes using

the MPN method

- to compare the results they exceed to the standard MPN

count

- provide information

- may give indication that the fishes are not properlyhandled

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Scope

Test only the fishes which are sold by fish endors

Only the skin and tissue of the fish will be tested

Only fecal coliform bacteria will be tested and will

be determined

Limitations Getting and testing fish samples from other area

Testing the water quality

Monitoring fish temperature

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MATERIALS

ice box

alcohol lamp

inoculating loop scalpel

surgical glo es

blender

test tubes

petri plates

durham tubes

MEDIA

L-EMB Broth

Lactose Broth

BGLB Broth

Peptone Water

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Tissue AnalysisGetting fish samples

Culture of Bacteria(lactose broth medium)

Transfer to another media(BGLB medium)

Recording of results

Obser ation

Analysis of data

Transfer to another media(L-EMB medium)

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TISSUE ANALYSIS

1pait

2tilapia

COMPOSITES

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Sample 1 TRIAL 1 TRIAL 2 TRIAL 3

10 ml 1 + + +

2 + + +

3 + + +

1.0 ml 1 + + +2 + + +

3 + + +

0.1 ml 1 + + +

2 + + +

3 + + +

LACTOSE BROTH (Presumptive Results)Sample 2 TRIAL 1 TRIAL 2 TRIAL 3

10 ml 1 + + +

2 + + +

3 + + +

1.0 ml 1 + + +2 + + +

3 + + +

0.1 ml 1 + + +

2 + + +

3 + + +

ALL LACTOSE BROTH TUBES

WERE POSITIVE.

RESULTS & DISCUSSIONS

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BGLB (Coliform Confirmation)

Sample1 TRIAL1 TRIAL2 TRIAL3

10 ml 1 + + +

2 + + +

3 + + +

1.0 ml1 + + +2 + + +

3 + + +

0.1 ml1 + + +

2 + + +

3 + + +

Sample2 TRIAL1 TRIAL2 TRIAL3

10 ml 1 + + +

2 + + +

3 + + +

1.0 ml1 + + +2 + + +

3 + + +

0.1 ml1 + + +

2 - + -

3 + + +

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SAMPLING DILUTION TRIAL

1 2 3

1 10-1

10-2

10-3

Dark pink colonies

Pink colonies

Metallic sheen

Metallic sheen

Metallic sheen

Metallic sheen

Pink colonies

Metallic sheen

Pink colonies

Metallic sheen, pink colonies

Metallic sheen

Pink colonies

Pink colonies

Pink colonies

Metallic sheen

Pink colonies

Pink colonies

Pink colonies

Pink colonies

Pink colonies

Pink colonies

Pink colonies

Pink colonies

Pink colonies

Pink colonies

Pink colonies

Pink colonies

2 10-1

10-2

10-3

Metallic sheen

Pink colonies

Dark pink colonies

Metallic sheen

Metallic sheen

Metallic sheen

Metallic sheen

Metallic sheen

Pink colonies

Pink colonies

Pink colonies

Metallic sheen

Pink colonies

Metallic sheen

Metallic sheen

Metallic sheen

Pink colonies

Pink colonies

Pink colonies

Pink colonies

Pink colonies

Pink colonies

Pink colonies

Pink colonies

Pink colonies

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Fecal coliform populations in the fishes were

high reaching more than 1,100 MPN-100g

which were beyond than the recommended

safety levels and prescribed limits of(Bureau ofFood and

Drugs).

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Base on the results, fishes sold in

along the highway of Balo-i arecontaminated with fecal coliform.