Figure S1
A
WM
1361
A
NRAS mutant
IKKε
Actin
WM
1346
WM
3670
WM
1366
WM
852
SK
Mel
2
SB
cl2
RA
W24
6.7
SK
Mel
173
SK
Mel
30
Figure S2
A - #2 - #2
WM1366 WM1346
B
C
0
***
*
20
40
60
80
100
WM1346 WM1361A WM1366 SBcl2
Per
cen
t M
igra
tion
of C
on
tro
l siCTL
siTBK1 #2
**
*
*
0
20
40
60
80
100
WM1346 WM1361A WM1366 SBcl2
Per
cen
t In
vasi
on
of
Co
ntr
ol siCTL
siTBK1 #2
siCTL
siTBK1
siCTL
siTBK1
ERK
TBK1
siTBK1
Figure S3
0 0.01 0.1 1 5
Aurora A Thr288Aurora B Thr222Aurora C Thr198
Aurora B
WM1366 TR TBK1-myr
AZ909 (μM)
IKKε
PDK1
phospho-PDK1 Ser241
Aurora A
0 0.01 0.1 1 5
SKMel2
phospho-Aurora A/B/C
Figure S4
AZ909AZD6244
- - + +- + - +
WM1366
siTBK1AZD56244
- - + +- + - +
WM1366
Bim-EL
Mcl-1
Bim-EL
Mcl-1
Bmf Bmf
BA C
siCTL
siTBK1
siCTL
siTBK1
AZD6244 DMSO
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