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Testing Stomatal Density in
LeavesAuthors deleted
Transpiration in Plants
Evaporation of water from stomata
Loss of water creates pulling force
Helps plants collect vital minerals
97% of water evaporates
Transpiration Rates
increase in higher temperature and wind
regulated by stomatal opening and closing
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Formulating Research Topic
Leaf thickness article:
Relates thickness/stomatal density to light intensity
Leaves in direct sun thicker more stomata
Plant-water relations article:
Stomatal density affects transpiration rates affect
photosynthesis rates
Light of low intensity less effective than stronger
illumination in inducing stomatal opening
Formulating Research Topic
Questions
Sun v. shade effect # of stomata?
Sun v. shade effect rate of transpiration?
• Hypothesis
Differences exist regarding stomatal density and
transpiration rates when Liquidambarst styraciflua(Sweetgum) is located in a sunny environment and when it
is located in a shady environment
• Prediction
Leaves in sunny environment have higher stomatal density
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Architecture of Experiment
Testing
Leaves
Sunny v. Shady
Conditions
SweetgumLiquidambarst styraciflua
•Large tree
•Easily identified by Leaves•Not native to California
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Methods & Materials
Test: Porometer measures stomatal conductance
1. Find Sweet gum in shade
2. Get reading, repeat for sun environment
3. 6 Readings in total!
Sample Size: 6 plants
3 in shade
3 in sun
Location: LMU School grounds
Statistics: T-Test
Methods & Materials
Test: Nail Polish Leaf Impression1. Acquire 6 sunny & 6 shady leaves
2. Coat polish on both halves
3. Place tape on polish, remove
4. Tape on slides, view under microscope
5. Count stomata
Statistics: T-Test
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Results
CalculatingStomataDensity
Totalstomata/F.O.V
mm^2
Results
Porometer P value = 0.0318
P < .05
Hypothesis Stands
• Stomata Count• P value= 1.25781E-07
• P < .05
Hypothesis Stands
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Conclusion
Differences exist b/w stomatal density of Sweet gum
leaves in a sunny v. shady environment
Higher stomatal density in sun
Lower in shade
T-Test Supports Claim
Prediction Correct
Leaves in sun need higher stomatal density to facilitate &
control transpiration rates since in direct sunlight.
Literature Cited
Terashima, I. "Irradiance and Phenotype: Comparative
Eco-development of Sun and Shade Leaves in Relation to
Photosynthetic CO2 Diffusion." Journal of Experimental
Botany 57.2 (2005): 343-54. Print.
Bernard S. Meyer, "Plant-water relations," in
AccessScience, ©McGraw-Hill Companies, 2008,
http://www.accessscience.com
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