Evolving Resistance:Research Goals and

Goods

Georgiana MayBrett CouchEthy Cannon

University of Minnesota

EducationTraining

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InformationGenome

Genes

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Evolution

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Resistance Resources

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Ever since Grube et al.

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Chromosome 11S

R3,7

I2

RB

I2- homologous sequences in Solanaceae

TomatoPotatoS. dem.EggplantPepperTobacco

Couch et al. MPMI 2006

PositiveSelection

I2- h NBS sequences under positive selection

NBS LRR

NBS and LRR regions reassociate through time

Contributions and resources

• Birth-death model applies to R gene evolution

• 232 sequences from 6 species– positive selection– evolving function? seek new resistance function in fewer than

10 lineages

• Brett Couch– David Schladt

I2 lineages maintained 12 million years

Ancientduplications

Contributions and resources

• Long term maintenance of R gene lineages - 12 my

• 232 sequences from 6 species– maintained through speciation – maintain function?seek non-host R in fewer than 10

lineages

• Brett Couch

I2-h map tochromosomes 8,9,11

in tomato

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8 9 11B

BB

B

IL Block B BAC contig

B

250 tomato BAC clones hybridized to I2 probes

Oligo probes hybridize to specifc I2 homologs

Map I2-containing BAC clonesto IL segment

BAC end polymorphisms segregate in the L. pinnellii mapping population

PCR length polymorphisms in parent lines

Contributions and resources

• Verified Pan, Fluhr results– chromosomes 8,9,11– not on 3,4 (Pan)

• New region - 9 I

• Ancient chromosomal duplications carry R gene duplications

• Brett Couch– Deren Eaton

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InformationGenome

Genes

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InformationGENOME

GENEOLOGY

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Baumgarten et al. 2002

R

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Ethalinda Cannon

solevol demo site

examples

• 75 distinct I2-h lineages– over 6 species– http://solevol.ccgb.umn.edu/

• Genome locations

• Development of evolutionary models

• Training

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Resistance Resources

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EducationTraining

€

InformationGenome

Genes

∫

Evolution

MapQuickTime™ and aTIFF (LZW) decompressorare needed to see this picture.QuickTime™ and aTIFF (LZW) decompressorare needed to see this picture.

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Resistance Resources

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