Evolving Resistance: Research Goals and Goods
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Evolving Resistance: Research Goals and Goods Georgiana May Brett Couch Ethy Cannon University of Minnesota
description
Evolving Resistance: Research Goals and Goods. Georgiana May Brett Couch Ethy Cannon University of Minnesota. Education Training. Evolution. Map. Resistance Resources. Chromosome 11S. Ever since Grube et al. I2. R3,7. RB. Tomato Potato S. dem. Eggplant Pepper Tobacco. - PowerPoint PPT Presentation
Transcript of Evolving Resistance: Research Goals and Goods
Evolving Resistance:Research Goals and
Goods
Georgiana MayBrett CouchEthy Cannon
University of Minnesota
EducationTraining
€
InformationGenome
Genes
∫
Evolution
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Resistance Resources
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Ever since Grube et al.
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Chromosome 11S
R3,7
I2
RB
I2- homologous sequences in Solanaceae
TomatoPotatoS. dem.EggplantPepperTobacco
Couch et al. MPMI 2006
PositiveSelection
I2- h NBS sequences under positive selection
NBS LRR
NBS and LRR regions reassociate through time
Contributions and resources
• Birth-death model applies to R gene evolution
• 232 sequences from 6 species– positive selection– evolving function? seek new resistance function in fewer than
10 lineages
• Brett Couch– David Schladt
I2 lineages maintained 12 million years
Ancientduplications
Contributions and resources
• Long term maintenance of R gene lineages - 12 my
• 232 sequences from 6 species– maintained through speciation – maintain function?seek non-host R in fewer than 10
lineages
• Brett Couch
I2-h map tochromosomes 8,9,11
in tomato
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8 9 11B
BB
B
IL Block B BAC contig
B
250 tomato BAC clones hybridized to I2 probes
Oligo probes hybridize to specifc I2 homologs
Map I2-containing BAC clonesto IL segment
BAC end polymorphisms segregate in the L. pinnellii mapping population
PCR length polymorphisms in parent lines
Contributions and resources
• Verified Pan, Fluhr results– chromosomes 8,9,11– not on 3,4 (Pan)
• New region - 9 I
• Ancient chromosomal duplications carry R gene duplications
• Brett Couch– Deren Eaton
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InformationGenome
Genes
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InformationGENOME
GENEOLOGY
∫
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Baumgarten et al. 2002
R
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Ethalinda Cannon
solevol demo site
• 75 distinct I2-h lineages– over 6 species– http://solevol.ccgb.umn.edu/
• Genome locations
• Development of evolutionary models
• Training
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Resistance Resources
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EducationTraining
€
InformationGenome
Genes
∫
Evolution
MapQuickTime™ and aTIFF (LZW) decompressorare needed to see this picture.QuickTime™ and aTIFF (LZW) decompressorare needed to see this picture.
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Resistance Resources
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