DNA Technologies from IGENITY
DNA Technologies from IGENITY
Dr. Stewart Bauck
Merial Company OverviewEstablished in 199717 Research and Development Centers16 Manufacturing SitesSales Presence in 150 Countries5,000 Employees WorldwideMerial Ltd. is a joint venture between Merck & Co., Inc. and sanofi-aventis.
The Brand
IGENITY® ProductsIGENITY DoubleBLACK™
IGENITY TenderGENE™
IGENITY OptiGRID™
IGENITY ParentMATCH™ MultiSIRE
IGENITY MultiMARK™
An accurate, inexpensive coat-color test:
Determines if an animal is homozygous black
A homozygous black bull can be worth as much as $800 more over his lifetime versus a heterozygous black bull when mated to red cows.
IGENITY DoubleBLACK
50% black calves $800100% black calves $1600Red Cows
75% black calves $1200100% black calves $1600Red Carrier Cows
100% black calves $1600100% black calves $1600Homozygous Black Cows
Red Carrier BullsHomozygous Black Bulls
• $3 to $5 per cwt. premium • 100 progeny weaned in bull’s lifetime• Sell 80 calves @ $20 premium (500 lbs.@ $4/cwt.) = $1600
IGENITY DoubleBLACKValue Proposition
IGENITY OptiGRID
A multiple marker analysis
Relationship to Carcass Traits– Yield Grade - 1.1 – Fat Thickness - .23 in. – Hot Carcass Weight - 86 lb. – Ribeye Area – 2.0 sq. in.
Each trait is scored 1 to 10
Marker-Assisted Management
Enter any starting backfat thickness and the desired backfat thickness at slaughter todetermine the days on feed required for each genotype.
OptiGRID 1 OptiGRID 5 OptiGRID 10Starting backfat 2.20 mm 2.00 mm 1.85 mm
Backfat at slaughter 12.00 mm 12.00 mm 12.00 mm
OptiGRID 1 OptiGRID 5 OptiGRID 10Starting backfat 0.087 in 0.079 in 0.073 in
Backfat at slaughter 0.472 in 0.472 in 0.472 in
Marbling Scores OptiGRID 1 OptiGRID 5 OptiGRID 10Current 3.90 3.73 3.60Target 5.00 5.00 5.00
Gain 0.79 0.71 0.62
Days on feed 132 d 153 d 174 d
Backfat and days on feed
0 mm
2 mm
4 mm
6 mm
8 mm
10 mm
12 mm
14 mm
0 d 50 d 100 d 150 d 200 d
Days on feed
Back
fat
thic
kness
42 days
Clear differences in times to finish have been demonstrated between IGENITYTM genotypes.
The New…
IGENITY TenderGENE
Three SNPs:
• CAPN316: Developed by U.S. Meat Animal Research Center (MARC). First released in 2003
• CAPN4751: Developed by MARC. Released in 2005
• UoG-CAST1: Developed by University of Guelph (Ontario, Canada). Released in 2005.
IGENITY TenderGENE
Calpastatin and CalpainBoth Are Important
Calpain: Enzyme that weakens muscle fibers during the post-mortem aging process.
Calpastatin: Enzyme that regulates the activity of Calpain.
-2.27 lb
-0.83 lb-1.44 lbWBSF
37%63%
Pct. of TotalCAPN316 and CAPN4751
Total
UoGCAST
Markers
Source: NBCEC, 2005
Genotypes in the shaded areas are rare. The assigned score is based on the best estimate of the tenderness value of this genotype given the information available today. The score for this genotype is subject to change as more information is obtained.
IGENITY TenderGENE ScoresUoG-CAST1 CAPN316 CAPN4751
10 CC CC CC -2.279 CC CG CC -1.958 CG CC CC -1.857 CC GG CC -1.637 CC CC CT -1.557 CC CG CT -1.557 CG CG CC -1.537 GG CC CC -1.446 CC GG CT -1.236 CG GG CC -1.215 CG CC CT -1.135 CG CG CT -1.135 GG CG CC -1.114 CC CC TT -0.834 CC CG TT -0.834 CC GG TT -0.834 CG GG CT -0.814 GG GG CC -0.794 GG CC CT -0.724 GG CG CT -0.723 CG CC TT -0.423 CG CG TT -0.423 CG GG TT -0.423 GG GG CT -0.401 GG CC TT 0.001 GG CG TT 0.001 GG GG TT 0.00
ScoreGenotype WBSF
Effect
05
1015202530
1 3 4 5 6 7 8 9 10IGENITY TenderGENE Score
IGENITY TenderGENE DistributionPe
rcen
t of P
opul
atio
n Scores 1 to 5 = 59%
Scores 6 to 10 = 41%
-3.0
-2.5
-2.0
-1.5
-1.0
-0.5
0.0
0.5
1.0
1 3 4 5 6 7 8 9 10
WBSF vs. IGENITY TenderGENE Score
IGENITY TenderGENE Score
Shea
r For
ce D
iffer
ence
(lb)
2.3 lb. difference
Red Angus, Charolais X Angus, Brangus, Brahman N=1209
Data derived from NBCEC Nov. 2005
More Tender
0%5%
10%15%20%25%30%35%40%
1 3 4 5 6 7 8 9 10IGENITY TenderGENE Score
Perc
ent >
11 lb
Data derived from NBCEC Nov. 2005
When Population average = 9 lb
Percent > 11 Pounds WBSF
More Tender
N=1209
9289915743344Brahman
Brangus
Red Angus
Char. X Ang.
Breed
534777232674307
455582182179219
46
C
CALP 4751 (%)
23
C
CALP 316 (%)
54772179339
TGGCNo.
UoG CAST1 (%)
Allele Frequencies by Breed
Data derived from NBCEC Validation, Nov. 2005
IGENITY TenderGENE
IGENITY™ Test ResultsPhone: 1-877-IGENITY www.igenity.come-mail: [email protected]
Ralph Smith Date: 3-12-06ABC Ranch Customer ID: USA20039123 Beef Road Invoice: 109034Anywhere, GA 12345 Sales Rep ID:
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This report describes results of our analyses of the sample or samples of biological materials described herein. Merial warrants that it has applied its best efforts to the determination of the presence and identification of specific alleles of bovine genes in DNA from the samples provided herein that were provided to us from you. Merial provides no other warrant including, but not limited to, that any other allele is or is not present in any sample or any animal. Results of analysis are predicated on the assumption that each sample was obtained from a single cattle beast. Results of analysis are reported herein in association with the sample designations provided by you. We assume no responsibility for correctly idenifying a particular animal as the source of any sample.
Animal ID Sex
Breed Registration
Number Sam
ple
Arc
hive
Sample Barcode Number Te
nder
GEN
E™ OptiGRID™
Qua
lity
Gra
de
Car
cass
Wei
ght
Rib
eye
Are
a
Yiel
d G
rade
Car
cass
Fat
Homozygous Black EDED
Breed DoubleBLACK™
• Improved accuracy for young sires• Spreads out the sires• Lower cost of genetic evaluation for traits difficult
and/or costly to measure• Other traits will follow
Marker Assisted EPDs
-0.02 0.92
YG acc
-0.36 0.50
0.01 0.92
0.00 0.92
.18 0.92
-8.4 0.92
Shear acc
REA acc
BF acc
Marbacc
CW acc
Spring 2005 ASA Sire Summary
IGENITY ParentMATCH
• Parental Validation
• Sire Determination
Parental Verification– Verify sire and/or dam as per breeder records.
Usually to fulfill breed association requirements.Listed parent either qualifies or is excluded.American Simmental and American Shorthorn Associations use a panel of 14 micro-satellite markers.Moving to “SNP” panels to allow parentage and diagnostic markers from the same sample
IGENITY ParentMATCH
Sire Determination– Multi-sire testing “Most
likely sire” based on screening potential sires and selecting the sire that has the most genes in common with each calf.
IGENITY ParentMATCH
Genetic Evaluation– Genetic evaluation
available on herd sires, using commercial parentage results.
– Available through collaboration with Colorado State University
– EPD’s for your sires in your environment, for traits that you want measured
IGENITY ParentMATCH
Large SNP panelAdvantages:
• All tests run up-front on all animals• Pay for what you want.• Higher throughput in the lab = lower lab costs= lower test costs.
• Expandable
Why IGENITY MultiMARK
IGENITY ParentMATCH
IGENITY DoubleBLACK
IGENITYTenderGENE
IGENITY OptiGRID
IGENITY MultiMARK
Using IGENITY Multiple test results from one sample for reasonable price.SimpleMeaningful“Friendly” ReportsValue (ROI)Part of a systemFocus on traits that are difficult and/or expensive to measure with significant economic relevance
Marker-Assisted EPDs from Genomic Data Develop the methodology
Standardized validation to generate “adjustment factors”
Standardized SNP nomenclature for genomic data
Standardized data transfer template
Producer authorization to transfer data to association
Breed association database acceptance
Two ways to order test kits:Online: www.igenity.comPhone: 877-443-6489
Get test results:Online: www.igenity.comEmailFaxUS Mail
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