DNA Technologies from IGENITY

DNA Technologies from IGENITY

Dr. Stewart Bauck

Merial Company OverviewEstablished in 199717 Research and Development Centers16 Manufacturing SitesSales Presence in 150 Countries5,000 Employees WorldwideMerial Ltd. is a joint venture between Merck & Co., Inc. and sanofi-aventis.

The Brand

IGENITY® ProductsIGENITY DoubleBLACK™

IGENITY TenderGENE™

IGENITY OptiGRID™

IGENITY ParentMATCH™ MultiSIRE

IGENITY MultiMARK™

An accurate, inexpensive coat-color test:

Determines if an animal is homozygous black

A homozygous black bull can be worth as much as $800 more over his lifetime versus a heterozygous black bull when mated to red cows.

IGENITY DoubleBLACK

50% black calves $800100% black calves $1600Red Cows

75% black calves $1200100% black calves $1600Red Carrier Cows

100% black calves $1600100% black calves $1600Homozygous Black Cows

Red Carrier BullsHomozygous Black Bulls

• $3 to $5 per cwt. premium • 100 progeny weaned in bull’s lifetime• Sell 80 calves @ $20 premium (500 lbs.@ $4/cwt.) = $1600

IGENITY DoubleBLACKValue Proposition

IGENITY OptiGRID

A multiple marker analysis

Relationship to Carcass Traits– Yield Grade - 1.1 – Fat Thickness - .23 in. – Hot Carcass Weight - 86 lb. – Ribeye Area – 2.0 sq. in.

Each trait is scored 1 to 10

Marker-Assisted Management

Enter any starting backfat thickness and the desired backfat thickness at slaughter todetermine the days on feed required for each genotype.

OptiGRID 1 OptiGRID 5 OptiGRID 10Starting backfat 2.20 mm 2.00 mm 1.85 mm

Backfat at slaughter 12.00 mm 12.00 mm 12.00 mm

OptiGRID 1 OptiGRID 5 OptiGRID 10Starting backfat 0.087 in 0.079 in 0.073 in

Backfat at slaughter 0.472 in 0.472 in 0.472 in

Marbling Scores OptiGRID 1 OptiGRID 5 OptiGRID 10Current 3.90 3.73 3.60Target 5.00 5.00 5.00

Gain 0.79 0.71 0.62

Days on feed 132 d 153 d 174 d

Backfat and days on feed

0 mm

2 mm

4 mm

6 mm

8 mm

10 mm

12 mm

14 mm

0 d 50 d 100 d 150 d 200 d

Days on feed

Back

fat

thic

kness

42 days

Clear differences in times to finish have been demonstrated between IGENITYTM genotypes.

The New…

IGENITY TenderGENE

Three SNPs:

• CAPN316: Developed by U.S. Meat Animal Research Center (MARC). First released in 2003

• CAPN4751: Developed by MARC. Released in 2005

• UoG-CAST1: Developed by University of Guelph (Ontario, Canada). Released in 2005.

IGENITY TenderGENE

Calpastatin and CalpainBoth Are Important

Calpain: Enzyme that weakens muscle fibers during the post-mortem aging process.

Calpastatin: Enzyme that regulates the activity of Calpain.

-2.27 lb

-0.83 lb-1.44 lbWBSF

37%63%

Pct. of TotalCAPN316 and CAPN4751

Total

UoGCAST

Markers

Source: NBCEC, 2005

Genotypes in the shaded areas are rare. The assigned score is based on the best estimate of the tenderness value of this genotype given the information available today. The score for this genotype is subject to change as more information is obtained.

IGENITY TenderGENE ScoresUoG-CAST1 CAPN316 CAPN4751

10 CC CC CC -2.279 CC CG CC -1.958 CG CC CC -1.857 CC GG CC -1.637 CC CC CT -1.557 CC CG CT -1.557 CG CG CC -1.537 GG CC CC -1.446 CC GG CT -1.236 CG GG CC -1.215 CG CC CT -1.135 CG CG CT -1.135 GG CG CC -1.114 CC CC TT -0.834 CC CG TT -0.834 CC GG TT -0.834 CG GG CT -0.814 GG GG CC -0.794 GG CC CT -0.724 GG CG CT -0.723 CG CC TT -0.423 CG CG TT -0.423 CG GG TT -0.423 GG GG CT -0.401 GG CC TT 0.001 GG CG TT 0.001 GG GG TT 0.00

ScoreGenotype WBSF

Effect

05

1015202530

1 3 4 5 6 7 8 9 10IGENITY TenderGENE Score

IGENITY TenderGENE DistributionPe

rcen

t of P

opul

atio

n Scores 1 to 5 = 59%

Scores 6 to 10 = 41%

-3.0

-2.5

-2.0

-1.5

-1.0

-0.5

0.0

0.5

1.0

1 3 4 5 6 7 8 9 10

WBSF vs. IGENITY TenderGENE Score

IGENITY TenderGENE Score

Shea

r For

ce D

iffer

ence

(lb)

2.3 lb. difference

Red Angus, Charolais X Angus, Brangus, Brahman N=1209

Data derived from NBCEC Nov. 2005

More Tender

0%5%

10%15%20%25%30%35%40%

1 3 4 5 6 7 8 9 10IGENITY TenderGENE Score

Perc

ent >

11 lb

Data derived from NBCEC Nov. 2005

When Population average = 9 lb

Percent > 11 Pounds WBSF

More Tender

N=1209

9289915743344Brahman

Brangus

Red Angus

Char. X Ang.

Breed

534777232674307

455582182179219

46

C

CALP 4751 (%)

23

C

CALP 316 (%)

54772179339

TGGCNo.

UoG CAST1 (%)

Allele Frequencies by Breed

Data derived from NBCEC Validation, Nov. 2005

IGENITY TenderGENE

IGENITY™ Test ResultsPhone: 1-877-IGENITY www.igenity.come-mail: IGENITYLabUSA@merial.com

Ralph Smith Date: 3-12-06ABC Ranch Customer ID: USA20039123 Beef Road Invoice: 109034Anywhere, GA 12345 Sales Rep ID:

1 345N M SM 29087456 129033894576 3 4 4 6 8 NA NA

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3

4

5

6

7

8

9

10

11

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1314

This report describes results of our analyses of the sample or samples of biological materials described herein. Merial warrants that it has applied its best efforts to the determination of the presence and identification of specific alleles of bovine genes in DNA from the samples provided herein that were provided to us from you. Merial provides no other warrant including, but not limited to, that any other allele is or is not present in any sample or any animal. Results of analysis are predicated on the assumption that each sample was obtained from a single cattle beast. Results of analysis are reported herein in association with the sample designations provided by you. We assume no responsibility for correctly idenifying a particular animal as the source of any sample.

Animal ID Sex

Breed Registration

Number Sam

ple

Arc

hive

Sample Barcode Number Te

nder

GEN

E™ OptiGRID™

Qua

lity

Gra

de

Car

cass

Wei

ght

Rib

eye

Are

a

Yiel

d G

rade

Car

cass

Fat

Homozygous Black EDED

Breed DoubleBLACK™

• Improved accuracy for young sires• Spreads out the sires• Lower cost of genetic evaluation for traits difficult

and/or costly to measure• Other traits will follow

Marker Assisted EPDs

-0.02 0.92

YG acc

-0.36 0.50

0.01 0.92

0.00 0.92

.18 0.92

-8.4 0.92

Shear acc

REA acc

BF acc

Marbacc

CW acc

Spring 2005 ASA Sire Summary

IGENITY ParentMATCH

• Parental Validation

• Sire Determination

Parental Verification– Verify sire and/or dam as per breeder records.

Usually to fulfill breed association requirements.Listed parent either qualifies or is excluded.American Simmental and American Shorthorn Associations use a panel of 14 micro-satellite markers.Moving to “SNP” panels to allow parentage and diagnostic markers from the same sample

IGENITY ParentMATCH

Sire Determination– Multi-sire testing “Most

likely sire” based on screening potential sires and selecting the sire that has the most genes in common with each calf.

IGENITY ParentMATCH

Genetic Evaluation– Genetic evaluation

available on herd sires, using commercial parentage results.

– Available through collaboration with Colorado State University

– EPD’s for your sires in your environment, for traits that you want measured

IGENITY ParentMATCH

Large SNP panelAdvantages:

• All tests run up-front on all animals• Pay for what you want.• Higher throughput in the lab = lower lab costs= lower test costs.

• Expandable

Why IGENITY MultiMARK

IGENITY ParentMATCH

IGENITY DoubleBLACK

IGENITYTenderGENE

IGENITY OptiGRID

IGENITY MultiMARK

Using IGENITY Multiple test results from one sample for reasonable price.SimpleMeaningful“Friendly” ReportsValue (ROI)Part of a systemFocus on traits that are difficult and/or expensive to measure with significant economic relevance

Marker-Assisted EPDs from Genomic Data Develop the methodology

Standardized validation to generate “adjustment factors”

Standardized SNP nomenclature for genomic data

Standardized data transfer template

Producer authorization to transfer data to association

Breed association database acceptance

Two ways to order test kits:Online: www.igenity.comPhone: 877-443-6489

Get test results:Online: www.igenity.comEmailFaxUS Mail