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Page 1: Development of broadband multispectral imaging for ... · Spot size analysis of diary products 500 550 600 650 700 750 800 850 0 100 200 300 400 500 600 Spot size [pixels] Wavelenght

Development of broadband multispectral imaging

for analysis of dairy products

Ph.D. student Otto H. A. Nielsen

Technical University of Denmark, department of InformaticsCentre for imaging food quality (CIFQ)

VideoMeterNKT - photonics

DANISCO

Photonex 2010 conference on spectral imaging3th November 2010

DTU - Informatics 1/18

Page 2: Development of broadband multispectral imaging for ... · Spot size analysis of diary products 500 550 600 650 700 750 800 850 0 100 200 300 400 500 600 Spot size [pixels] Wavelenght

Outline

1 SLS based hyperspectral imaging

2 Illumination system

3 Preliminary results

4 Outlook

DTU - Informatics 2/18

Page 3: Development of broadband multispectral imaging for ... · Spot size analysis of diary products 500 550 600 650 700 750 800 850 0 100 200 300 400 500 600 Spot size [pixels] Wavelenght

Outline

1 SLS based hyperspectral imaging

2 Illumination system

3 Preliminary results

4 Outlook

DTU - Informatics 3/18

Page 4: Development of broadband multispectral imaging for ... · Spot size analysis of diary products 500 550 600 650 700 750 800 850 0 100 200 300 400 500 600 Spot size [pixels] Wavelenght

Spectral imaging for food diagnostics

DTU - Informatics 4/18

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Spectral imaging for food diagnostics

Vision systems

Structural sampleinformation

Characterisation ofbiological samples

Spectroscopy

Chemical analysis

DTU - Informatics 4/18

Page 6: Development of broadband multispectral imaging for ... · Spot size analysis of diary products 500 550 600 650 700 750 800 850 0 100 200 300 400 500 600 Spot size [pixels] Wavelenght

Spectral imaging for food diagnostics

Vision systems

Structural sampleinformation

Characterisation ofbiological samples

Spectroscopy

Chemical analysis

Advantages for food analysis:

Fast

Non-invasive

⇒ Suitable for in line process inspection

DTU - Informatics 4/18

Page 7: Development of broadband multispectral imaging for ... · Spot size analysis of diary products 500 550 600 650 700 750 800 850 0 100 200 300 400 500 600 Spot size [pixels] Wavelenght

Inspection method for homogeneous products milk,

cream, yoghurt etc.

Illumination sources, a collimated beamSub surface light scattering (SLS)

DTU - Informatics 5/18

Page 8: Development of broadband multispectral imaging for ... · Spot size analysis of diary products 500 550 600 650 700 750 800 850 0 100 200 300 400 500 600 Spot size [pixels] Wavelenght

Inspection method for homogeneous products milk,

cream, yoghurt etc.

Illumination sources, a collimated beamSub surface light scattering (SLS)

Scattering of biologicalparticles is described myLorentz-Mie theory.

DTU - Informatics 5/18

Page 9: Development of broadband multispectral imaging for ... · Spot size analysis of diary products 500 550 600 650 700 750 800 850 0 100 200 300 400 500 600 Spot size [pixels] Wavelenght

Inspection method for homogeneous products milk,

cream, yoghurt etc.

Illumination sources, a collimated beamSub surface light scattering (SLS)

Scattering of biologicalparticles is described myLorentz-Mie theory.

Can determine:

Particle size

Particle concentration

Light absorption

Light scattering

DTU - Informatics 5/18

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Previous measurements performed by Danisco1.

1On-line monitoring of food processes using subsurface laser scatteringDTU - Informatics 6/18

Page 11: Development of broadband multispectral imaging for ... · Spot size analysis of diary products 500 550 600 650 700 750 800 850 0 100 200 300 400 500 600 Spot size [pixels] Wavelenght

Previous measurements performed by Danisco1.

1On-line monitoring of food processes using subsurface laser scatteringDTU - Informatics 6/18

Page 12: Development of broadband multispectral imaging for ... · Spot size analysis of diary products 500 550 600 650 700 750 800 850 0 100 200 300 400 500 600 Spot size [pixels] Wavelenght

Previous measurements performed by Danisco1.

The structure of the sample relates it’s optical properties. Thecharacterisation method will be described later.

1On-line monitoring of food processes using subsurface laser scatteringDTU - Informatics 6/18

Page 13: Development of broadband multispectral imaging for ... · Spot size analysis of diary products 500 550 600 650 700 750 800 850 0 100 200 300 400 500 600 Spot size [pixels] Wavelenght

Outline

1 SLS based hyperspectral imaging

2 Illumination system

3 Preliminary results

4 Outlook

DTU - Informatics 7/18

Page 14: Development of broadband multispectral imaging for ... · Spot size analysis of diary products 500 550 600 650 700 750 800 850 0 100 200 300 400 500 600 Spot size [pixels] Wavelenght

Illumination source

Light generation with a broad spectral profile

SuperK light source

Produces a bright output from 500 nm to 2500 nm, and the beamrequires optical filtering to be used in spectral imaging.

DTU - Informatics 8/18

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Optical filtering ⇒ hyperspectral beam

Acousto-optical tunable filter (AOTF)

Can cover the spectrum from500 nm to 2500 nm with twoAOTF crystals

Returns two beams, one with visible light and a one for the NIR.

DTU - Informatics 9/18

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Optical filtering ⇒ hyperspectral beam

Acousto-optical tunable filter (AOTF)

Can cover the spectrum from500 nm to 2500 nm with twoAOTF crystals

Returns two beams, one with visible light and a one for the NIR.

DTU - Informatics 9/18

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Final light delivery

The light is delivered using an micro structured fiber (LMA5).

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Wavelength [nm]

Bea

m w

aist

m]

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0.1

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0.5

Wavelength [nm]

beam

pow

er [

mW

]

DTU - Informatics 10/18

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Schematically setup

DTU - Informatics 11/18

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Schematically setup

Novel features of the illuminations system

An small fibre output → easy to handle

Collimated Gaussian beam → simple scattering pattern

Broad spectral range (500 nm to 900 nm) → chemicalspecificity

Fast switching of wavelengths → usable for industrialinspection

DTU - Informatics 11/18

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Outline

1 SLS based hyperspectral imaging

2 Illumination system

3 Preliminary results

4 Outlook

DTU - Informatics 12/18

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Image example with whole milk, λ = 670 nm

DTU - Informatics 13/18

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Image example with whole milk, λ = 670 nm

DTU - Informatics 13/18

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Image example with whole milk, λ = 670 nm

The scattering can be characterised by the slope of the loglogcurve and the spot size of the saturated.

DTU - Informatics 13/18

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SLS slope characteristics of diary products

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3x 10

−3

SLS

Slop

[ar

b. u

nit]

Wavelenght [nm]

Skimmed milk 0.5 %letmælk 1.5%Whole milk 3%

Can discriminate betweenproducts of different fat contents.

DTU - Informatics 14/18

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SLS slope characteristics of diary products

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0.5

1

1.5

2

2.5

3x 10

−3

SLS

Slop

[ar

b. u

nit]

Wavelenght [nm]

Skimmed milk 0.5 %letmælk 1.5%Whole milk 3%

Can discriminate betweenproducts of different fat contents.

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0.5

1

1.5

2

2.5

3x 10

−3

SLS

Slop

[ar

b. u

nit]

Wavelenght [nm]

yoghurt 3 % fatCream 9 % fatCream 18 % dilutedCream 18 % fat

Different slope for same fatcontents

DTU - Informatics 14/18

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Spot size analysis of diary products

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Spot

siz

e [p

ixel

s]

Wavelenght [nm]

Skimmed milk 0.5 %letmælk 1.5%Whole milk 3%

DTU - Informatics 15/18

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Spot size analysis of diary products

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Spot

siz

e [p

ixel

s]

Wavelenght [nm]

Skimmed milk 0.5 %letmælk 1.5%Whole milk 3%

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Spot

siz

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ixel

s]Wavelenght [nm]

yoghurt 3 % fatCream 9 % fatCream 18 % dilutedCream 18 % fat

DTU - Informatics 15/18

Page 28: Development of broadband multispectral imaging for ... · Spot size analysis of diary products 500 550 600 650 700 750 800 850 0 100 200 300 400 500 600 Spot size [pixels] Wavelenght

Spot size analysis of diary products

500 550 600 650 700 750 800 8500

100

200

300

400

500

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Spot

siz

e [p

ixel

s]

Wavelenght [nm]

Skimmed milk 0.5 %letmælk 1.5%Whole milk 3%

500 550 600 650 700 750 800 8500

50

100

150

200

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300

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400

450

Spot

siz

e [p

ixel

s]Wavelenght [nm]

yoghurt 3 % fatCream 9 % fatCream 18 % dilutedCream 18 % fat

Line crossings at long wave lengths, showing interesting features.

DTU - Informatics 15/18

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Outline

1 SLS based hyperspectral imaging

2 Illumination system

3 Preliminary results

4 Outlook

DTU - Informatics 16/18

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Future implementations

Analyse products with simple changes (fat contents andparticle size). Make correlation between the SLS spectrumand the food property based on statistical models.

Combining the illumination system with a robot to move thefibre. This will make it possible to examine non symmetricscattering distribution and surface reflection.

Perform instrument independent characterisation of thesamples intrinsic scattering parameters.

DTU - Informatics 17/18

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Acknowledgements

The work presented is sponsored by the The Danish Council forStrategic Research

Coworkers:

Anders Dahl - post.doc. DTU IMM - projectguidance and data analysis

Videometer - imaging equipment and dataanalysis

NKT photonics - construction and tuning of theillumination system

Danisco - introduction to food analysis

DTU - Informatics 18/18