1000bp1000bp

Tamyb10-A (CS)

Tamyb10-RP4Tamyb10-LP7

Tamyb10-A (Norin 17)

GeST (2.2 kb)

Tamyb10-LP8 Tamyb10-RP7

Tamyb10-A (Norin 61)

Tamyb10-LP6Tamyb10-RP6

94 °C × 5 min↓94 °C × 30 sec64 °C × 30 sec 30 cycles72 °C × 30 sec↓72 °C × 7 min

94 °C × 5 min↓94 °C × 30 sec61 °C × 30 sec 30 cycles72 °C × 30 sec↓72 °C × 7 min

94 °C × 5 min↓94 °C × 30 sec61 °C × 30 sec 30 cycles72 °C × 2 min↓72 °C × 7 min

a

b

c

19bpdeletion

Tamyb10-LP2 Tamyb10-RP1

94 °C × 5 min↓94 °C × 30 sec61 °C × 30 sec 30 cycles72 °C × 30 sec↓72 °C × 7 min

Tamyb10-B (CS)

Tamyb10-RP3Tamyb10-LP9

94 °C × 5 min↓94 °C × 30 sec64 °C × 30 sec 30 cycles72 °C × 1 min↓72 °C × 7 min

Tamyb10-D (CS)

d

e

sense primer antisense primerNorin 61 CS Norin 17

R-A1b R-A1a R-A1a

Tamyb10-A1 (1) Tamyb10-LP6 Tamyb10-RP6 665 bp none 665 bp

Tamyb10-A1 (2) Tamyb10-LP7 Tamyb10-RP4 none 536 bp none

Tamyb10-A1 (3) Tamyb10-LP8 Tamyb10-RP7 565bp 565bp 2750bp

sense primer antisense primerNorin 61 CS

R-B1b R-B1a

Tamyb10-LP2 Tamyb10-RP1 282bp 263bp

sense primer antisense primerCS Norin 17

R-D1b R-D1a

Tamyb10-LP9 Tamyb10-RP3 1353bp none