1000bp
1
1000bp 1000bp Tamyb10-A (CS) Tamyb10-RP4 Tamyb10-LP7 Tamyb10-A (Norin 17) GeST (2.2 kb) Tamyb10-LP8 Tamyb10-RP7 Tamyb10-A (Norin 61) Tamyb10-LP6 Tamyb10-RP6 94 °C × 5 min ↓ 94 °C × 30 sec 64 °C × 30 sec 30 cycles 72 °C × 30 sec ↓ 72 °C × 7 min 94 °C × 5 min ↓ 94 °C × 30 sec 61 °C × 30 sec 30 cycles 72 °C × 30 sec ↓ 72 °C × 7 min 94 °C × 5 min ↓ 94 °C × 30 sec 61 °C × 30 sec 30 cycles 72 °C × 2 min ↓ 72 °C × 7 min a b c 19bp deletion Tamyb10-LP2 Tamyb10-RP1 94 °C × 5 min ↓ 94 °C × 30 sec 61 °C × 30 sec 30 cycles 72 °C × 30 sec ↓ 72 °C × 7 min Tamyb10-B (CS) Tamyb10-RP3 Tamyb10-LP9 94 °C × 5 min ↓ 94 °C × 30 sec 64 °C × 30 sec 30 cycles 72 °C × 1 min ↓ 72 °C × 7 min Tamyb10-D (CS) d e sense primer antisense primer Norin 61 CS Norin 17 R-A1b R-A1a R-A1a Tamyb10-A1 (1) Tamyb10-LP6 Tamyb10-RP6 665 bp none 665 bp Tamyb10-A1 (2) Tamyb10-LP7 Tamyb10-RP4 none 536 bp none Tamyb10-A1 (3) Tamyb10-LP8 Tamyb10-RP7 565bp 565bp 2750bp sense primer antisense primer Norin 61 CS R-B1b R-B1a Tamyb10-LP2 Tamyb10-RP1 282bp 263bp sense primer antisense primer CS Norin 17 R-D1b R-D1a Tamyb10-LP9 Tamyb10-RP3 1353bp none
description
a. Tamyb10-A ( Norin 61 ). Tamyb10-RP6. 1000bp. 1000bp. Tamyb10-LP6. b. Tamyb10-A ( CS ). Tamyb10-LP7. Tamyb10 - RP 4. c. GeST (2.2 kb). Tamyb10-A ( Norin 17). Tamyb10 -LP8. Tamyb10 -RP7. 19bp. deletion. d. Tamyb10-B (CS). Tamyb10 -LP2. Tamyb10 - RP 1. e. Tamyb10-D - PowerPoint PPT Presentation
Transcript of 1000bp
1000bp1000bp
Tamyb10-A (CS)
Tamyb10-RP4Tamyb10-LP7
Tamyb10-A (Norin 17)
GeST (2.2 kb)
Tamyb10-LP8 Tamyb10-RP7
Tamyb10-A (Norin 61)
Tamyb10-LP6Tamyb10-RP6
94 °C × 5 min↓94 °C × 30 sec64 °C × 30 sec 30 cycles72 °C × 30 sec↓72 °C × 7 min
94 °C × 5 min↓94 °C × 30 sec61 °C × 30 sec 30 cycles72 °C × 30 sec↓72 °C × 7 min
94 °C × 5 min↓94 °C × 30 sec61 °C × 30 sec 30 cycles72 °C × 2 min↓72 °C × 7 min
a
b
c
19bpdeletion
Tamyb10-LP2 Tamyb10-RP1
94 °C × 5 min↓94 °C × 30 sec61 °C × 30 sec 30 cycles72 °C × 30 sec↓72 °C × 7 min
Tamyb10-B (CS)
Tamyb10-RP3Tamyb10-LP9
94 °C × 5 min↓94 °C × 30 sec64 °C × 30 sec 30 cycles72 °C × 1 min↓72 °C × 7 min
Tamyb10-D (CS)
d
e
sense primer antisense primerNorin 61 CS Norin 17
R-A1b R-A1a R-A1a
Tamyb10-A1 (1) Tamyb10-LP6 Tamyb10-RP6 665 bp none 665 bp
Tamyb10-A1 (2) Tamyb10-LP7 Tamyb10-RP4 none 536 bp none
Tamyb10-A1 (3) Tamyb10-LP8 Tamyb10-RP7 565bp 565bp 2750bp
sense primer antisense primerNorin 61 CS
R-B1b R-B1a
Tamyb10-LP2 Tamyb10-RP1 282bp 263bp
sense primer antisense primerCS Norin 17
R-D1b R-D1a
Tamyb10-LP9 Tamyb10-RP3 1353bp none
