WP5 - MICROBIAL METABOLIC ACTIVITIES CNRS DR 12 - LMGEM
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Transcript of WP5 - MICROBIAL METABOLIC ACTIVITIES CNRS DR 12 - LMGEM
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WP5 - MICROBIAL METABOLIC ACTIVITIESCNRS DR 12 - LMGEM
BACTERIAL ACTIVITIES
* STRICT ANAEROBIC ACTIVITIES* Methane Production (MP)* Sulfate Reduction (SR)
* BACTERIAL PRODUCTION & ACTIVITY* Bacterial Production (BP)* Glutamate Uptake* Chemotrophic production* Hexadecane respiration
Contract EVK3-2000-00042
D. MARTY, J. GARCIN, C. TAMBURINI, A. BIANCHI
BIODEEP Meeting IX MILANO 17-19 May 2004
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Microbial activities measured Glutamate uptake, Bacterial & Chemolithotrophic production,
Hexadecane respiration
* Heterotrophic bacterial production representative of the processes due to the whole heterotrophic microbial community
* Chemolithotrophic production biomass production by chemolitrophic organisms growing without organic enrichment, and using CO2 as C source for biomass production * Assimilation and respiration of glutamate used as a rough index of the ratio linking bacterial biomass production processes to those used to produce energy * Hexadecane respiration occasionally measured as a paragon of ability for microbial populations to mineralize hydrocarbon compounds
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Bacterial production in the Upper interfacesrelatively to the salinity
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pmol C l-1 h-1
S (
%)
Urania
L'Atalante
Bannock
Discovery
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Microbial activities within brines Heterotrophic bacterial & Chemolithotrophic production
Heterotrophic bacterial production
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Urania L’Atalante Discovery Bannock
pm
ol C
l-1
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Chemolitotrophic production
ND ND
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Microbial activities within brines Glutamate assimilation and respiration, Hexadecane
respiration
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pm
ol
C l
-1 h
-1
00,20,40,60,8
11,21,41,61,8
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Urania L’Atalante Discovery Bannock Urania L’Atalante Discovery Bannock
ND ND
Glutamate assimilationGlutamate respiration
Hexadecane respiration
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Microbial activities in near-bottom water
Heterotrophic bacterial & Chemolithotrophic production
Heterotrophic bacterial production
Chemolitotrophic production
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15
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25
30
35
pm
ol C
l-1
h-1
Urania L’Atalante Discovery Bannock
ND NDNo NBB available
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Microbial activities in near-bottom water
Glutamate assimilation and respiration, Hexadecane respiration
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pm
ol
C l
-1 h
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Urania L’Atalante Discovery Bannock Urania L’Atalante Discovery Bannock
ND No NBB available No NBB availableND
Glutamate assimilationGlutamate respiration
Hexadecane respiration
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Microbial activities in the superficial sediments
Glutamate assimilation and respiration, Hexadecane respiration
Glutamate respiration with 1 µM initial concentration
Glutamate respiration with 5 µM initial concentration
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pm
ol C
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h-1
Hexadecane respiration with 5 µM initial concentration
Urania L’Atalante Discovery Bannock
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Toward the research of piezophile hydrocarbonoclastic bacteria from the
Urania Basin
* HP 1 10 mL inoculum brine + 100 mL sterilized brine + 40 µL Hexadecane + 20 mg (NH4)3PO4 + 8mM Na2S (ANOXIC CONDITIONS)
* HP 2 10 mL inoculum interface + 100 mL sterilized interface + 40 µL Hexadecane + 20 mg (NH4)3PO4
* HP 3 10 mL inoculum brine + 100 mL ONR7a + 40 µL Hexadecane + 20 mg (NH4)3PO4
* HP 4 10 mL inoculum interface + 100 mL ONR7a + 40 µL Hexadecane + 20 mg (NH4)3PO4
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Toward the research of piezophile hydrocarbonoclastic bacteria from the
Urania Basin
Inoculum(10 mL)
Medium(100 mL)
Oxic conditio
n
Hexadecane
(NH4)3PO4
HP 1 BrineStrilized
brineANOXIC(8mM Na2S)
40 µL 20 mg
HP 2 InterfaceStrilized interface
OXIC 40 µL 20 mg
HP 3 Brine ONR7a OXIC 40 µL 20 mg
HP4 Interface ONR7a OXIC 40 µL 20 mg
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Toward the research of piezophile hydrocarbonoclastic bacteria from the
Urania Basin
Pressure = 35 MPa
Agitation table
HP bottles
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Toward the research of piezophile hydrocarbonoclastic bacteria from the
Urania Basin
Results from Giuseppe D’Auria
After several months of incubation RNA (16S cDNA) extraction of subsamples of main culture show that an active population is living and growing in high-pressure conditions in each condition tested.
100bp Marker
HP bottles1 2 3 4
Cloning and sequencing are in progress…
PCR gel of 16s cDNA amplification
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What about our article in AEM?
Submission in September 2002
Response in January 2003 Major revisions : improve the English language, physical and chemical parameters, numbers of bacteriaPotential authors : Gasparonni, D’Auria for TC, chemist?,
May 2004 It would be very important to include other authors to improve the paper and corroborate some hypotheses we did
Contract EVK3-2000-00042
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What about our article in AEM?
Data from cruise I
Focus: Evidence of microbial life in DHABs
Contract EVK3-2000-00042
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What about our article in AEM?
Contract EVK3-2000-00042