www.intertek.com

Yvonne Ferguson10/04/23

Projects laboratory

Prepared by Yvonne Ferguson

www.intertek.com

What we do

Biocide trialsTo determine biocidal effect of products supplied by our clients

FFUBR trialsTo study effect of different treatment regimes on reservoir souring

Other projectsTo find solutions to specific problems relevant to our work, or our clients

Corrosion projectsTo determine efficacy of corrosion inhibitors on MIC (Microbial Influenced Corrosion)

Prepared by Yvonne Ferguson

www.intertek.com

Biocide Trials

Prepared by Yvonne Ferguson

Different types of biocideA large trial with eg 16 chemicals at 3 concentrations and 3 timepoints

• Minimum of 148 MPNs, including Time 0 and Control

• 296 boxes of SRB media, 296 boxes of GHB media

• 2368 needles. 24 boxes.

• People to help with inoculations. Takes approximately 10 minutes to inoculate 4 boxes of media.

• Space to set media boxes out on benches

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Biocide Trials

PlanktonicBacteria are cultured from seawater, or water supplied by client, and subjected to biocide treatment. The most effective biocide is determined by MPN count by inoculating cell suspension into media.

Prepared by Yvonne Ferguson

www.intertek.com

Biocide Trials

SessileBacteria is grown as biofilm on a surface of known area ie sessile bacteria grown on biostuds. Biofilm grown on biostuds is subjected to biocide treatment. The most effective biocide is determined by MPN count.

Prepared by Yvonne Ferguson

www.intertek.com

Biocide Trials

Dynamic Flow CellBiofilm grown on biostuds, placed in a flow cell and biocide solution flowed through the cell to determine biocide efficiency.

Prepared by Yvonne Ferguson

www.intertek.com

Biocide Trials

• Minimum of 148 MPNs, including Time 0 and Control

• 296 boxes of SRB media, 296 boxes of GHB media

• 2368 needles. 24 boxes.

• People to help with inoculations. Takes approximately 10 minutes to inoculate 4 boxes of media.

• Space to set media boxes out on benches

ScaleA large trial with eg 16 chemicals at 3 concentrations and 3 timepoints

ValueA large trial with eg 16 chemicals at 3 concentrations and 3 timepoints may cost c. £50, 000

Prepared by Yvonne Ferguson

www.intertek.com

FFUBR trials

FFUBRFixed Film Upflow Bio Reactor Aka ‘sandpacks’

Investigate effect of nutrient additions, nitrate or biocide treatment

Water is flowed continuously through a glass bead or sand packed column. Flow is measured daily to detect any problems which need remedied.

Effluent from the columns is analysed for sulphide, sulphate, iron, pH, nitrate, nitrite, DNA, FISH, MPN, VFA.

Prepared by Yvonne Ferguson

www.intertek.com

FFUBR trials

Prepared by Yvonne Ferguson

www.intertek.com

FFUBR Trials

• Treating with nitrate to encourage growth of NRB (nitrate reducing bacteria) and inhibit growth of SRB

• Investigating whether pulse treating with nitrate is more effect than continuous treatment with nitrate. This would have cost benefits for the customer.

• Treating with biocide to inhibit bacterial growth

• Investigating if treatment with eg nitrate or biocide can assist with oil recovery from the reservoirs.

Examples

ValueA typical trial with one test column and one control column may cost £3000 per month and run for a minimum of 2 months though ideally should run for longer to gain decent results.

Prepared by Yvonne Ferguson

www.intertek.com

Corrosion Projects

Corrosion trialsLPR (linear polarising resistance) probe can directly measure corrosion rate of a solution and generate real-time graphs.

Processing Corrosion couponsWeight lossPhotographyPittingCorrosion RateSulphideBacteria count

Prepared by Yvonne Ferguson

www.intertek.com

Corrosion Trials

Supporting our customers in their global trade

Our organisation The industries we operate in What we doOur organisation What we doThe industries we operate in

Processing coupons Before cleaning After cleaning

Photo Letter

LocationCoupon Number

Coupon Material

Coupon TypeSurface

AreaDensity

Install Date

Remove Date

Expose Time Days

Expose Time

Hours

Blank Initial

Weight

Blank Final

Weight

Blank Weight Loss

Initial Weight

Final Weight

Weight Loss

Corrected Weight Loss

Corrosion Rate

Max Pit Depth

Pitting Rate

(cm2) (g/cm3) (g) (g) (g) (g) (g) (g) (g) (mmpy) (mm) (mmpy)

A BP454503 M1850 C1018 3" Strip 33.6 7.86 29-Sep-12 11-May-13 224 5376 36.7726 36.7686 0.0040 37.3343 35.8777 1.4566 1.4526 0.090 0.010 0.016

B BP454503 M1853 C1018 3" Strip 33.6 7.86 29-Sep-12 11-May-13 224 5376 36.7726 36.7686 0.0040 37.5001 36.1354 1.3647 1.3607 0.084 0.040 0.065

C CP454504 11 L80 -13Cr 3" Strip 33.6 7.7 29-Sep-12 11-May-13 224 5376 35.2487 35.2461 0.0026 35.4425 35.4270 0.0155 0.0129 0.001 0.000 0.000

D CP454505 53 L80 -13Cr 3" Strip 33.6 7.7 29-Sep-12 11-May-13 224 5376 36.3230 36.3114 0.0116 36.9982 36.9432 0.0550 0.0434 0.003 0.000 0.000

Due to the nature of the chemical cleaning there may be occasions where weight loss of blank is marginally higher than analysed coupon. In event of such cases the corrected weight loss will be reported as zero.

Qualitative Categorisation of Carbon Steel Corrosion Rates for Oil Production Systems from NACE Standard RP0775-2005

Low

Moderate

High

Severe

Average Corrosion Rate (mmpy)

Client:Platform/Site:Laboratory Work No:

OceaneeringBP MungoF1225/0386

Maximum Pitting Rate (mmpy)

< 0.13

0.13-0.20

0.21 - 0.38

> 0.38

< 0.025

0.13 - 0.25

0.025 - 0.12

> 0.25

Prepared by Yvonne Ferguson

www.intertek.com

Corrosion trials

Our organisation What we do

Prepared by Yvonne Ferguson

www.intertek.com

SRB-mediated corrosion of carbon steel

Prepared by Yvonne Ferguson

www.intertek.com

Very low levels

• ie (µg/l) probably no effect, possible inhibition of CO2 corrosion

Low levels

• ie (mg/l) effect on corrosion, possible cracking

Higher levels

• ie (g/l) high risk of stress cracking

At some point the system might become ‘sour’

Prepared by Yvonne Ferguson

www.intertek.com

Other projects

Projects past and present:

• Assessment of risk of microbially influenced corrosion in mud associated with mooring chains.

• Trial to determine the efficacy of UV against bacterial populations (including SRB) in seawater

• Evaluation of effect of silver based products on numbers of sessile sulphate-reducing bacteria

• Evaluation of the effect of various clays on the anaerobic activity in septic tanks

Prepared by Yvonne Ferguson

www.intertek.com

Day –to Day

• Quality tasks – temperature monitoring, pipette calibration, equipment maintenance, stock control

• Admin tasks – ordering materials, stock control, meetings

• Writing COSHH and risk assessments for work done in the lab

• Maintenance of ongoing projects and trials – sampling from sandpack columns, reading media, subculturing bacteria

• Report writing and test certificate preparation

Prepared by Yvonne Ferguson

Prepared by Yvonne Ferguson