VARIA Abstracts Congress OHDMBSC 2008 - Oral...

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1. BEHAVIOUR ASPECTS OF ORALHYGIENE IN CARDIOVASCULARPATIENTSAuthors: Sanda Mihaela Popescu, Gabriela Pãtroi,Adina Glodeanu, Adina Magdalena BungetFaculty of Dental Medicine, University of Medicine andPharmacy of Craiova

Aim: The study had the objective to determine thelevel of knowledge and attitude towards oralhygiene in a group of patients with different cardio-vascular illnesses. Methods: A group of 150 patients aged 62.32(10.41) with different cardiovascular diseases(hypertension, coronary heart disease, chronic car-diac insufficiency) was included in the study. Theyanswered to a questionnaire about oral hygiene andits importance for general health. Results: Once daily dental brushing is performedby 44% of patients, while 24% of them neverbrushed. Only 24% patients from the group report-ed professional education about dental brushing.Regarding last dental visit, 28% patients presentedto the dentist five years ago, 24% patients were tothe dentist 10 years ago and 16% patients havenever done dental check-ups. Only 24% patientsfrom the group presented to the dentist in the lastyear, but for emergency dental care and also only8% patients have done preventive dental visits.Although 96% patients from the group have neverbeen scaled, only 60% from them have complainedabout gingival bleeding. From these, 40% havemade some correlation between inaccurate dentalbrushing and gingival bleeding. Only 40% patientsfrom the group considered that oral hygiene andcardiovascular illness could have been related, and28% patients could not have made a connectionbetween the general health status and oral hygiene. Conclusions: There is an important need for a bet-ter collaboration between the cardiologist, the fam-ily doctor and the dentist in order to make thepatients fully aware of the impact of oral health ongeneral health status, underlining preventive dentalcontrol importance in oral and general health.

2.THE INCREASE OF TITANIUMIMPLANTS BIOCOMPATIBILITY BYANODIC OXIDATIONAuthors: Ruxandra Sfeatcu, Ioan Roman, Cornel Fra-tila, Mihai Burlibasa, Laura Soare, Corina Cristache

”Carol Davila”University, Faculty of DentalMedicine, Bucharest, Romania;METAV R&D,020011 C.A.Rosetti st.31, Bucharest, Romania,3National Institute for Nonferrous and Rare Metals,Bucharest, RomaniaIntroduction: Titanium and his alloys are exten-sively used in medicine, since 1960, as surgicalimplants and biomedical prosthetic devices, due tohis high strength-to-weight ratio and high corrosionresistance in the human body. The objective of thispaper is to realise, on titanium and his TA6V4alloy, an oxidic, nanostructural layers with highadherence, chemical stability and biocompatibility.Material and methods: The oxidic layer was pro-duced by anodic oxidation in aqueous acidic andalkaline electrolytes, in one or few stages, and bythermal processing at three different temperatures.The anodic oxidation and thermal processing opti-misation was realised by anticorrosive performanc-es evaluation by cyclic voltametry in Ringer solu-tion. Results: The obtained ceramic structures passivatespontaneously and are electrochemically inactiveuntil high anodic potentials, beginning from thefirst polarisation cycle. The passivity currents arevery low and the anodic oxidation processes areirreversible. The scanning electron microscopyanalysis of the oxides layers showed an alveolarmorphology, favourable to the nucleation andgrows of bone tissue. The X-ray diffraction analy-sis, the high resolution electron microscopy(HRTEM) and selected area electrons diffraction(SAED) showed the presence of anatas and rutiletype titanium oxides in the layers, and their nanos-tructure too.Conclusions: The paper proposes a performant andcomplex way to increase the biocompatibility oftitanium and his alloy TA6V4 and contends theutility of the electrochemical techniques of investi-gation in the optimisation of surface technologiesand materials. Poster

3. HELPING WAYS OF GROWING THEEFFICIENCY OF THE SUPPORT AREAFOR ORTHODONTIC PURPOSEAuthors: R. Stanciu, Anca Temelcea, I. Simion, D.Stanciu, B. Stanciu, I. Popovici; U.M.F. „CarolDavila” Bucureºti, România, Universitatea TituMaiorescu Bucureºti, România, Clinica deOrtodonþie ºi Ortopedie Dento-facialã, Clinica de

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Abstracts Congress OHDMBSC 2008

implantologie, Catedra Noþiuni de ProteticãDentarã, Disciplina Tehnica Metalo-ceramicã.Orthodontic use of mini-implantsThe force equilibrium between active and passiveparts of the dental arch in the orthodontic patientshas always been a challenge for the practitioners,who innovated and/or invented new techniques tomaster it. The degree of compliance of the patienthas always been a factor of treatment efficiency.The less compliance we need, the better we cancontrol the appliance. Here we present a new ortho-dontic implant patented by Bucharest OrthodonticDepartment, and some case reports. Key words:mini-implant, cooperation doctor-patient, efficien-cy, brevet.

4. THE PSYCHOLOGICAL AND ECONOMI-CAL EFFICIENCY OF THE CONSCIOUSSEDATION METHOD WITH MEOPA INTHE PEDIATRICAL STOMATOLOGY ATCLUJ-NAPOCAAuthors: Nicoleta Statov1, Hermiona Vigh3, E.Cocarla1, A. Serbanescu1, J. Nancy2; 1 UMF Cluj-Napoca, Romania, 2 Victor Segalen University,Bordeaux, France, 3Sibiu,

Objectives: The estimations of the psychologicaland economical efficiency of the conscious seda-tion method with MEOPA in the pediatrical stoma-tology Methods:I Subjects: 403 children between4-12, selected from the children who have come fora medical consultation in a pediatrical stomatologyconsultation room from Cluj-Napoca.Psichological dependent variables: the fear of den-

tist, the acceptance of the dental medical treatment,coping, and the efficiency of the coping percep-tion.The independent variables: each child’s thera-peutic intervention was preceded and accompaniedby concious sedation with MEOPA.The child was assisted psychologically with a prop-er method for his psychological profile and for therecommended medical treatment.The method ofdeterming the dependent psychological variables:fear was determined with the help of CFSS-DSquestionnaire, the attitude of acceptance with thehelp of Sarmat Scale the coping tyle and the per-ception of its efficiency with the help of DCQ ques-tionnaire. The research aimed at the dependent psy-chological variables before and after the therapeu-tic intervention.II For the same subjects the costs, the income andeconomic benefit were estimated using the eco-nomic management methodology.Results: The significant reduction of the level of

fear is accompanied by the changing of the copingstyle. Conclusions: The method of conscious sedation ofchildren through MEOPA method is economicallyand psychologically effective.Key words: MEOPA, conscious sedation,

5. ASSESSMENT OF THE PREVALENCE OFDENTAL FEAR AND ITS CAUSES AMONGCHILDREN AND ADOLESCENTSAuthors: Anca Maria Rãducanu, ReghinaAnghelescu, Victor Ion Feraru; Department ofPediatric Dentistry, UMF Carol Davila Bucharest

Introduction: Dental fear is a common occurrence,an essential and inevitable emotion that appears asa response to the stress induced by various dentalprocedures. Its intensity varies from nervousnessand anxiety to dental phobia and is considered themain barrier to a successful completion of treat-ment.Objectives: Evaluation of the dental fear/anxiety inchildren and adolescents, as well as the motivesthat lead to their appearance.Material:The present study was conducted on a group ofpatients with ages between 1 and 18 years, all ofwhom were consulted and treated in theDepartment of Paediatric Dentistry of UMF „CarolDavila” in Bucharest. Method: Dental fear wasmeasured from the doctors’, the patients’ and theparents’ perspective, using the following scales:clinical correlated with Frankl’s scale for the doc-tor and facial image scale (FIS) for children of 3 to18 years of age and their parents. Results: The results of the study point to the exis-tence of negative behaviour towards dental proce-dures in a significant proportion in children andadolescents. Its main causes are fear of pain, gener-alized fear of doctors with a particular emphasis ondental practitioners.Notable differences between the evaluation of thestate of fear done by the doctors/parents and thepatients’ self evaluation were observed.Conclusions: Measure of dental fear is an extreme-ly useful tool for the dental practitioner, who willthus be able to individualise the required behav-ioural treatment.

6. CHARACTERISTICS OF LYMPHOCYTICINFLAMMATORY INFILTRATE IN BASALCELL CARCINOMA OF THE ORAL ANDMAXILLOFACIAL AREAAuthors: Albertine Leon1, Zenaida Ceauºu2, MihaiCeauºu3, Carmen Ardeleanu4, Teofil Mehedinþi5

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OHDMBSC - Vol. VII - Supplement - June, 2008

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1 Faculty of Dental Medicine and Pharmacy,“Ovidius” University of Constanþa2 “Sf. Pantelimon” Clinical Hospital, Bucharest 3 Department of Pathology, “Victor Babeº”National Institute of Pathology, “Carol Davila”University of Medicine and Pharmacy, Bucharest4 “Victor Babeº” National Institute of Pathology,“Carol Davila” University of Medicine andPharmacy, Bucharest5 Faculty of Medicine, “Ovidius” University ofConstanþa.

Background: Basal cell carcinoma (BCC) is a veryfrequent skin malignancy, with slow evolution andrare metastases. Host tissues react to tumor inva-sion through complex inflammatory response,comprising varied inflammatory cells. Materialand method: We assessed the expression of lym-phocytes in 37 archived formalin-fixed paraffin-embedded tissue samples of BCC from the oral andmaxillofacial region by means of immunohisto-chemical (IHC) method, using the SABC (strepta-vidin biotin complex) indirect tristadial techniquefor UCHL1, CD4, CD8 and L26 markers.Inconclusive cases were eliminated. Results:Lymphocytes were found in great numbers in BCCstroma, displaying nodular, linear or scattered dis-position within tumor stroma. The positivity toUCHL1 in T lymphocytes was 100%, for CD4 in Thelper (Th) lymphocytes – 62.96%, for CD8 in Tcytotoxic (Tc) lymphocytes – 92% and for L26 inB lymphocytes – 76%. We found a statistically sig-nificant negative correlation between Th/CD4+ andTc/CD8+ lymphocytes (r = - 0.54, p = 0.004).Conclusion: The results of our research demon-strate the presence of abundant lymphocyticinflammatory infiltrate in BCC stroma. The pre-dominance of T cells in front of B cells suggests theextensiveness of cell-mediated immune response,rather than a humoral immune reaction. Tc lymphocytes were prevalent as against Th lym-phocytes, which evidence that the immune mecha-nism prevalent in BCC is of cytotoxic type.Keywords: basal cell carcinoma, lymphocytes,UCHL1, CD4, CD8, L26.

7. STUDY OF IMMUNOLOGICAL MARK-ERS IN PAROTID TUMORS Authors: Carmen Defta, Silviu Alecu, BogdanDabu, Silvia DumitriuMicrobiology Department; “Carol Davila” DentalSchool of Medicine, Bucharest, Romania

Parotid gland is a very important lymphatic organwhich, through its secretory activity, exhibits a keyrole in oral cavity defense. It is already known thatparotid gland secretes the highest quantity of IgA (sIgA) but also IgG, IgM which have an importantrole in oral cavity defense process against localpathological agents.The aim of the study was to determine the presenceand quantifying some of the importantimmunoglobulines and alfa 1 anti-trypsin immuno-logical factor as well in parotid tumors. Material and methods: Blood samples were har-vested from 88 patients with parotid tumors andfrom 20 healthy donors who constitute the controllot. The samples were performed using Mancinitechnique (radial simple immunodifusion). Finaldata were statistically analyzed by performing stu-dent t-test.Results: All tested immunoglobulines (IgA, Ig Gand Ig M) show high values in most of the investi-gated tumors. The exceptions were IgA showingup a lower value in spinocellular carcinoma ascompared with the controls as well IgG in undif-ferentiated carcinoma. There were detectedincreased serological values of alfa 1 anti-trypsininin the following tumor types: pleomorph adenoma,Wathin tumor, adenocarcinoma, undifferentiatedcarcimoma and ductal carcinoma.Conclusion : In parotidian deseases the investigat-ed immunoglobulins and alfa 2 antitripsin, seems tohave a clinical importance, their of neoplasma far-ther investigation are needed to see the value of thisimmunoglobulin in clinical prediction also theirrelevance the diagnosis of other parotidian neoplas-ma. Key words: immunological markers, parotidtumors, IgA, Ig G, Ig M, alfa 2 anti-trypsin

8.CHANGING OF IL2, IL6, TNFααCYTOKINES IN PAROTID TUMORSAuthors: Carmen Defta, Silviu Alecu, DanielaPetrusca*, Anca Dumitriu, Silvia DumitriuMycrobiology Department; “Carol Davila” DentalSchool of Medicine, Bucharest, Romania, * IndianaUniversity Pulmonary, Allergy, Critical Care andOccupational Medicine, Indianapolis, USA.POSTER

Parotid gland is responsible for a complex processof immunological defense in oral cavity, thus anymodification of its activity can determine an imbal-ance of local immune response highly involved.The aim of this study was to determine the pres-ence and to quantify the expression of IL2, IL6,

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TNF alfa cytokines in parotid tumors.Material andmethods: Blood samples were harvested frompatients with parotid tumors and from healthydonors. IL2, IL6, TNF alfa cytokines were meas-ures by ELISA technique following the manufac-turer’s protocol. Results: In most of carcinomasand lymphomas were detected high levels of IL2and IL 6 followed by the adenomas as comparedwith the controls. Increased TNF values weredetected only in the investigated carcinomas cases.Conclusions: The proliferal transformations oftumor cells is highly correlated the increasing plas-matic level of IL2, IL6, TNF alfa cytokines withmeaning a tendency of the immune system to mon-itories the neoplasic process and as well a localimmune imbalance which may cause furtherevents.Key words: parotid gland, parotid tumors, bio-cenosis, cytokines, IL2, IL6, TNF alfa.

9.THE OSTEOGENIC POTENTIAL OFEMBRYONIC STEM CELLSAuthors: Ondine Lucaciu, D. Gheban, OlgaSoritau, Mihaela Baciut, D. Lucaciu, R. Campian,B. Crisan, M. Aschilean ,G. Baciut., University ofMedicine and Pharmacy, Cluj-Napoca

Introduction: The seeding of embryonic micestem cells on a scaffold obtained from red deerdecidous horn, could form an osteogenetic com-plex, that could be used in grafting bone defects.Purpose: This study evaluated the osteogeneticpotential of a tissue engineered bone using embry-onic mice stem cells seeded on a scaffold obtainedfrom the red deer decidous horn.Materials and Methods:The embryonic micestem cells where from R1/E/NA cell line providedby Dr. Elen Gocza, Godollo, Hungary. We startedculturing the cells on scaffolds obtained from reddeer decidous horn using passive seeding in flaskscontaining basal or complex osteogenic medium.Cell differentiation in vitro was assessed biochem-ically by alkaline phosphatase activity. The tissueengineered constructs were implanted in bonedefects surgically induced in the left parietal boneof the mices and ectopically.Bone formation invivo was quantified by histologic examination at 2and 4 months after in vivo grafting.Results: Culturing the cells in osteogenic mediumon the scaffold, induced bone formation, showedby high alkaline phosphatase activity.The tissue engineered constructs produced ectopicbone tissue at low frequency and amounts. The

microscopic evaluation of the tissue, generated inthe defect, demonstrated bone formation.Conclusions: Our data indicates that embryonicmice stem cells can be cultured on red deer deci-dous horn, the engineered complex is osteogenic.

10.HEMORHEOLOGICAL CHANGES INMAXILLOFACIAL TRAUMAAuthors: Gabriela Lilios, Cristina Farcas, ZiziNiculescu, Mihaela Ghinea, Violeta Sapira, MirceaGrigorian, Ileana Ion, Cecilia Adumitresi, CarmenCiufu, Sanda Jurja, Loredana Pazara, PhysiologyDepartment of „Ovidius” University, Faculty ofDental Medicine, Constanta

The dynamics of the mechanisms involved in thetraumatic shock is an issue that interests both thephysiopathologists and the clinicians and the inves-tigations made so far indicate a practical accessibil-ity and have distinct importance in the diagnosis,therapeutic control and forecasting evolution of thepatients with maxillofacial trauma.The aim of this work is to demonstrate the erythro-cyte aggregability modifications in polytraumatisedpatients.The determinations were produced on two groups:the control group (which comprises 30 healthy chil-dren) and the test group (that includes a number of30 polytraumatic children), observed in twomoments of polytraumatic evolution, namely whenhospitalized and after 7 days. The erythrocytes fil-terability test, described by Teitel, was used as amethod in studying erythrocyte aggregability. The decrease of erythrocyte filtered volume, deter-mined in the moment of hospitalization and after 7days, making a comparison between the children oftest group and control group, confirms the increaseof acute phase reactants and the forming of inter-erythrocyte bounds which will establish the rise oferythrocyte aggregability in traumatic shock.The modifications of erythrocyte aggregability anddeformability that appear in the state of shock deter-mine changes at the level if sanguine density, withmicrocirculatory hemorheological perturbationwhich drives disorders of the oxygenating capacityof tissues and of the local metabolic processes alongwith all consequences resulting from this fact.

11. TEM STUDIES OF CRYSTALLINEHYDROXYL AND FLUORO-APATITE INBONE TISSUESAuthors: Aureliana Caraiane, V. Ciupina, G. Prodan,S. Zamfirescu, M. Prodan,I. Stanescu, V. Mocanu, C.

Stefanov, E. Vasile; Ovidius University of Constanta,Bucharest. POSTERWe study the presence of apatite on different bonetissues and characterize this material by electronmicroscopy and additional techniques. First groupof tissue was dental tissues, which exhibit a crys-talline form of hydroxyl and fluoro-apatite.Electron diffraction and XRD data confirm pres-ence of polycrystalline like material. The morpho-logical characteristics of tissue are investigated byusing TEM micrograph and show apatite nanocrys-tal arranged around osteocyte cell that are apatitegenerator in tissue. The dimension of this cell isalso investigated. Also the bone tissue is examined.Samples are prepared for TEM by included smallpiece of material in epoxy resin and cut thin sectionby means of ultramicrotome equipped with dia-mond knife. Thickness of ultrathin sections are keptaround 50-60nm. The size of sections are keepsmall, around 500 ìm and less, to eliminate the arti-fact that appear on section due to hardness of mate-rial. Structural data was completed by examine thechemical composition by means of EDX tech-nique.Keywords: TEM, XRD, EDX, apatite, osteocyte

12. ORODENTAL MODIFICATIONS INPATIENTS WITH SACCHRATES DYA-BETES. CLINICAL AND STATISTICALSTUDY. I- st PARTAuthors: Vârlan Constantin, Dana Cristina Bodnar,Vãideanu Teodor, Virginia Vârlan, Bodnar Traian,Mariana Brânduºa Popa. Department ofRestorative Odonthoterapy, Faculty of dentalMedicine, U.M.F.” Carol Davila” Bucharest.

Aim: In this paper we have research the incidenceof diabetes insulin dependent form I or non-insulindependent form II, in lot of 1500 patients bothmales and females, aged between 31 and over 90years, in-patients between 2004 and 2007 with dif-ferent diagnostics, in clinics from Bucharest. Wehave also considered the in our study the risk fac-tors associated with the basic disease.Method: The interdisciplinary clinical-statisticalstudy, realized on the lot of 1500 patients had con-sist in an elaborately and interdisciplinary clinicalexam of specialty based on a research paper whichincluded dates of identity of patient, the basic dis-ease diagnostic, personal and familial case-history,existing risk factors, teeth and periodontal lesions,oral mucous lesions, variations of salivary secre-

tion, estimation of oral hygiene, the dates beingtooled statistically.The results of the study have revealed the existenceof 183 patients with diabetes (12, 13%) among of 20with form I and 163 with form II. At these patientswe revealed the presence of some associated riskfactors and the main are hyper blood pressure, dis-orders of lipids metabolism, obesity, etc. Conclusions: The variety of patients who searchfor specialty treatment in dental clinics is verylarge, the age, sex, educational level, professionand general diseases being only few of the vari-ables, every patient being a specific entity. An enti-ty like this is the diabetic patient who has a highincidence and his treatment must be personalized,considering the particularities of his disease. The plurality of factors: the ability of making a cor-rect oral hygiene, sometimes limited, the postpone-ment of dental consult until the appearance of anemergency, the particularities of getting ill specificfor the disease and the consequences of its treat-ment, the way of landing the patient from the den-tist part are just some of the characteristics of dia-betic patients.Key words: diabetes, risk factors, interdisciplinarystudy

13. ORODENTAL MODIFICATIONS INPATIENTS WITH SACCHRATES DYABETES. CLINICAL AND STATISTICALSTUDY. II –nd PART.Authors: Vârlan Constantin, Dana Cristina Bodnar,Bodnar Traian, Virginia Vârlan, Vãideanu Teodor,Mariana Brânduºa Popa. Department ofRestorative Odonthoterapy, Faculty of dentalMedicine, U.M.F.” Carol Davila” Bucharest.

Aim: This paper presents, on base of a clinical-sta-tistical study, the frequency of orodental lesionsand the oral mucous lesions found at patients fromthe lot with diabetes form I or form II. Through ourstudy we aimed to identify the ways of approachingthe diabetic patient in the ordinary conditions of adental clinic; this ways are depending on the type ofdental treatment in order to be made and on thegravity of the disease.Method: The interdisciplinary clinical-statisticalstudy made on the lot of 1500 patients had consist-ed in an elaborately and interdisciplinary clinicalexam of specialty based on a research paper andhad studied the presence of caries and of dentalwear lesions, the frequency of tooth missing, the

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pathology of marginal periodontium and the role ofsalivary variations in this. Results: The degenerative modifications of theperiodontal blood vessels, the quantitative decreaseof salivary secretion, the increase of the concentra-tion of glucose in the fluid of the gingival slot andin the secretion of the parotid gland, reducing theactivity of phagocytosis of the neutrofils, determinethe severity of dental and periodontal affectionsand also the incidence of microbial or mycosis oralinfections. Conclusions: The alimental habits determine themetabolic disorders, one of this being the diabeticmetabolic syndrome which is represented by acomplex of disorders which includes the disordersof lipids metabolism, the hyper blood pressure, andthe abdominal obesity. These influence the appear-ance of series of oral disease such as the caries, theperiodontal disorders, and the lesions of oralmucosa. The relation between diabetes, obesity,cerebral and cardiovascular diseases and the statusof orodental health can be put on the sight of thepersonal life style.Key words: diabetes, dental and periodontal disor-ders, oral mucosa xerostomy. And hypo salivarysecretion.

14. ANTHROPOMETRICAL STUDYINCLUDING A GROUP OF STUDENTSFROM CONSTANTA DENTAL MEDICINEFACULTYAuthors: D.F. Petrovici, Cristina Gabriela Puºcaºu,Viorica Roxana Teodorescu, Faculty of DentalMedicine, “Ovidius” University Constanta,Romania.

Background: The study is part of an inter-univer-sity research program aimed to design the dentalwork place based on human-centered ergonomicsconcept. Purpose: To realize the data base forestablishing the requirements of dental equipment.Method and material: The study group included50 subjects, aged between 20-36 years old, studentsfrom Constanta Dental Medicine Faculty, who rep-resent future dentists. A special anthropometricalchart was elaborated including 12 dimensions fororthostatic and sitting work position. Results: Thesex distribution shows that females representmajority, 64% from the total group. The average ofmain dimensions measured is represented by thefollowing results: in orthostatic position- 170,28cm medium height, 107,28 cm elbow-floor; in sit-

ting position- 69,92 cm elbow-floor distance, 75,32cm eyes-sitting surface height dimension.Conclusions: The present data base will be extend-ed and correlated with the others of TimisoaraDental Medicine Faculty and Bucharest DentalMedicine Faculty, in order to elaborate the anthro-pometrical model used to project the future dentalequipment.

15. THE QUALITY OF CHRONICAL DISEASE MONITORING IN ROMANIA, IN THE EUROPEAN INTEGRATION CONTEXT

Authors: Dana Galieta Minca1; D. Moraru21 University of Medicine and Pharmacy, Bucharest,Romania-Department of Public Health andManagement, Prof Dr. Department Chief2 National House of Health Insurance, Bucharest,Romania, Expert to the Medical Service , SpecialistDr of Public Health and Management

A monitoring system implementation of publichealth services and data collecting from publichealth field represents an old concern at Europeanlevel. Lately, more and more countries are interest-ed by the quality evaluation of these monitoringsystems. In this context, the aim of this study is to increasethe quality of monitoring the chronicle diseases inorder to ensure and improve the health status of thepopulation. The aim of this study is to analyze the impact ofusing the practical protocols and the SingleIntegrated Informatics System on monitoring thechronic diseases.The study was realized by analyzing the data con-cerning to the patients’ treatment with some chron-ic disease, for whom are founded central commit-tees of experts at the National Health InsuranceHouse level. These are referring to:

1. Antiviral treatment in viral hepatitis B, C, Dand to the patients with hepatic cirrhosis;2. Treatment for rheumatoid poliarthritis, psori-azical arthropathy and juvenile idiopatic arthri-tis;3. Gaucher disease treatment.

The hypotheses considered were:1. Using the practical protocols determines theincrease of treatment efficiency and efficacy ofthe chronic disease.2. The Single Integrated Informatics Systemincreases the quality of diseases’ monitoring.

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The total number of the patients who benefit ofstandardized treatment and who were brought inthe study, respectively those who were diag-nosed with chronic disease for whom existsapproved practical protocols, were of 8026, asfollowing:

- 3500 patients are during an antiviral treat-ment and 2500 patients are on the waiting listfor the chronic viral hepatitis B, C and D andfor hepatic cirrhosis HBV, C and D;- A number of 2000 patients are during a treat-ment for rheumatoid poliarthritis, psoriazicalarthropathy and idiopatic juvenile arthritis;- A number of 20 patients are during the treat-ment and a number of 6 patients are on a wait-ing list for the Gaucher disease.

The study was made between September-October2007.As a result of data analysis, work hypothesis wereconfirmed as following:

- It is realized the standardization of the diag-nostically and therapeutically procedures;- It is guaranteed the population access to thequality medical services;- It is assured a better control of the diseases inthe population and, implicit, in their treatment,because of the realized monitoring by intro-ducing of the Single Integrated InformaticsSystem;- The costs are lower because of the elimina-tion the useless investigations;- By the realized reports having adjuvant thecropped data by Single Integrated InformaticsSystem introduction, it could be made estima-tions regarding the budget for the health statusassurance and maintenance of the population.

As a result obtained from the study made, there areimposed the following:

1. Elaboration and approving of practical proto-cols in other cases of chronic disease that treat-ment is approved by the territorial committees.2. The assurance of the needed informatics tech-nology and preparing the involved personal formonitoring the chronic disease.3. Medical informatics monitoring system har-monization of the diseases with those existent atthe European level.

16. ROMANIAN IMPLEMENTATION OF EULEGISLATION IN FOOD FIELDAuthors: Daniela Nuþã – Institute of Public HealthBucharest – Health and Environment Department;

Nicolae Nuþã – Authority of Public Health Bucharest–Environment of Assessment Factors DepartmentThe Romanian food legislation has been transposedand harmonized in accordance with EC legislation(directives, regulations, decisions etc).Bellow isrepresented some of the normatives from the foodfield: - Government Decision no. 106/2002 regardingfood labelling, with next amendments, which trans-poses the Directive 2000/13/EC relating to thelabelling, presentation and advertising of foodstuffswith the amendments;- Order of Public Health Ministry no. 1069/2007for approval of the Norms regarding food supple-ments which transposes the Directive 2002/46/ECrelating to food supplements and the Directive2006/37/EC which modifies the annex no. 2 of theDirective 2002/46/CE ;- Order of Family and Health Ministry and of

Agricultural, Food and Forestry Ministry no.387/251/2002 for approval of the Norms regardingfoodstuffs for particular nutritional uses, withamendments and changes, which transposes theCouncil Directive 89/398/EEC relating to food-stuffs intended for particular nutritional uses,amended by Directive 96/84/EC and Directive99/41/EC ; Directive 91/321/EEC on infant formu-lae and follow-on formulae, amended by Directive96/4/EC and Directive 99/50/EC; CommissionDirective 96/5/CE on processed cereal-based foodsand baby foods for infants and young children,amended by Directive 98/36/CE and Directive99/39/CE; Commission Directive 1999/21/EC ondietary food for special medical purposes andCommission Directive 1996/8/EC on foods intend-ed for use in energy-restricted diets for weightreduction.In accordance with these norms there aredefined:The label means any words, particulars, trademarks, brand name, pictorial matter or symbolrelating to a foodstuff and placed on any packaging,document, notice, label, ring or collar accompany-ing or referring to such foodstuff. Food supplements means foodstuffs the purposeof which is to supplement the normal diet andwhich are concentrated sources of nutrients or othersubstances with a nutritional or physiologicaleffect, alone or in combination, marketed in doseform, namely forms such as capsules, pastilles,tablets, pills and other similar forms, sachets ofpowder, ampoules of liquids and powders designedto be taken in measured small quantities.

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Foodstuffs for particular nutritional uses arefoodstuffs which, owing to their special composi-tion or manufacturing process, are clearly distin-guishable from foodstuffs for normal consumption,which are suitable for their claimed nutritional pur-poses and which are marketed in such a way as toindicate such suitability. In conclusion, these are some examples of thetransposition of the legislation that must beknowned and implemented by the operators onfood industry.

17. RESULTS OF FIRST 9 MONTHS OFNATIONAL HEALTH EVALUATION INDOLJ COUNTY ROMANIAAuthors: Iuliana Manuela Dragomir, Lucian PaulDragomir; University of Medicine and Pharmacyof Craiova, Romania.

Aim: Main tasks of this program is to collect dataabout health status, to offer 5 to 9 free investigationto all person’s evaluate according to their risk andfinally to appreciate the risk for most communemajor disease (cardiovascular diseases, osteoporo-sis, cervical cancer, breast cancer, colorectal can-cer, diabetes mellitus).Method: In Romania startedfor the first time a National Program of HealthEvaluation, in July 2007 initially for a year period.This program is for all Romanian citizens now mat-ter if they have or not social insurance. This pro-gram is happening now in Romania in primary careby 10 983 general practitioners (GPs) for a yearperiod: July 2007- July 2008. In Dolj county howhas a population of 730 000 citizens, are includedin National Program of Health Evaluation all 408GPs and 40 para-clinic providers. We have theresults from the first 9 months (July 2007- March2008). In order to evaluate the health status thepopulation was informed to participated to this pro-gram by post, and they go to their GPs in the monththey have born, were the general practitioners con-sult them, addressed questions about health statusand send them to the laboratories for specific inves-tigations.During the last 9 months, where evaluat-ed a total of 325 391 citizens. The addressability todoctor was only 62% from Dolj population.Persons with social insurance represent 86,66%from all persons, the rest have now health insur-ance. Results: The results shows the high level ofrisk for all chronic diseases investigated in popula-tion ( total risk is 50,20% to develop a diseases), thegreater risk was for diabetes mellitus-almost 30%from population have this risk and cardiovascular

risk was calculated in Dolj county in this period to10,92%. Conclusions: This evaluation program it was anecessary solution to know the real prevalence ofchronic illness in population and, to register per-sons who has high risk of illness, and to generatedata for future health programs.Key-words: Health evaluation, Dolj County.

18. ETHICAL ASPECTS FROM THELABOUR MEDICINE PRACTICEAuthors: D. Fotache, Colentina Clinical HospitalBucharest, President of A.P.M.M.M.R.;. LetiþiaBucur, Institute of Public Health Bucharest; CarmenNeagoe Braºov; R. Ionuþ, U.M.F. “Iuliu Hatieganu”Cluj-Napoca; Brânduºa Constantin, U.M.F. “Gr.T.Popa” Iaºi; E. Vancu, “Aurel Vlaicu” University,Arad, I. Belcin, Constanþa.

PATRONAGE ASSOCIATION OF WORK-MEDICINE DOCTORS FROM ROMANIA(A.P.M.M.M.R), founded in 2004, reunites about40 specialists and primary doctors of work-medi-cine from all over the country, which have foundedoffices, centers, own services of work-medicine orare affiliated to some polidisciplinar medical cen-ters. They are practicing work-medicine part-timeor full-time in private. This is the “patronage” char-acter of the Association, recognized by theChamber of Commerce and Industry of Romaniaand the Romanian Patronage where is affiliated. The professional and scientific character is includ-ed in the status of the association and aims for theactivity improving, adoption of well practice stan-dards, communication and promoting on the topicthat reunites us, work health and security.Romanian Medical College accredited us as EMCproviders, not only for the declared intentions, butalso on the experiences already known in workingof these activities.Private practice in Work Medicine raises some eth-ical, deontological and medical-legal problems;these are a source of:

- conflict on the relation employer-employee-doctor;- professional hierarchy degradation (primarydoctor, specialist doctor, professor)- medical act alteration by adopting of a non-involving attitude or formal invlolving;- juridical or administrative files;- Practices of unfair competition.

Unsuccessful try to impose a law of the Doctor of

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Work-Medicine Status, 418/2004, inapplicable,versus Ethical and Deontological Code ofICOH/CIST – founded in 1906 – produces a high-er confusion of the involved actors and the consis-tent dilution of our measure: the preservation of theemployee health status.As a consequence, Romania has the lowest numberof allowanced professional diseases in U.E., about2200 cases yearly, and the highest number of mor-tal work accidents, 487 deads/2007. The numbersare illustrating the absence of an occupationalhealth strategy, generated too by the confused leg-islative measure of the two responsible – Ministryof Health and Ministry of Work. The communication shows ethical aspects on con-crete examples, cropped and signalized by authorsfrom Bucharest, Brasov, Iasi, Cluj, Constanta.

19. TERRITORIAL INEQUALITIES INDEMOGRAPHIC INDEX OF HEALTH STA-TUS EVALUATIONAuthors: Mihaela Cristina Dusciuc, AlexandraCucu, Janta Denisa; UMF”Carol Davila” Bucharest;2 Department of Public Health and SanitaryManagement

Background. Reliable information on populationhealth status and characteristics is a fundamentalrequirement for the public health decision-makers.The objective of the study envisages identifyingdifferences between the demographic rates in the42 counties of Romania.Methods. The study is a transversal descriptiveanalysis. Variables for the database were collectedfrom the following data sources: National Instituteof Statistic and National Center for Organizationand Support of Information and Informatics Systemof Health. The reference year of the study was2006. The identification of disparities was based onquartile calculation and cartogram representation.The rates used in this study belong to vital statistics(live-births and mortality). The following indica-tors on fertility were used: crude birth rate, crudereproduction rate, average age of mother at allbirths, abortion ratio (the number of abortions per1000 live births), abortion rate per 1 women; themortality indicators used were: age-standardizedmortality rate, mortality rates by residence (rural,urban), age-standardized mortality rates by cause(cardiovascular diseases, respiratory tract diseases,digestive diseases, cancer, injuries), life expectancyat birth and potential years of life lost per 1000 pop-ulation.

Results. The counties were divided by quartiletechnique (Q1, Q2, Q3) in four groups of equal fre-quency. First group includes the counties withfavorable indicators, second and third groupsinclude the counties with indicators close to coun-try averages and fourth group includes the countieswith unfavorable indicators. Particular issues foreach of the counties are shown in synthetic tables.Conclusions. This simple and feasible approachallows us the detection of health issues for each ofthe counties and the detection of counties withhealth concerns (several health issues).

20. SWOT ANALYSIS OF LEGISLATIVEFRAMEWORK ON THE SUPERVISIONAND CONTROL OF COMMUNICABLE DISEASES IN ROMANIAAuthors: Denisa Janta, Alexandra Cucu, DanielaPitigoi, Mihaela Dusciuc, University of Medicineand Pharmacy Carol Davila, Bucharest,

Introduction. Communicable diseases similar toother medical international treats for the populationhealth are not influenced by geographic borders. Inthis context, excepting vaccination preventableinfectious diseases, surveillance remains the maininstrument of medical intervention for preventionof communicable diseases.Material and methods. This study consists in aSWOT analysis of the Romanian legislature by theperspective of general and specific characteristicswhich might influence implementation and appro-priatness of measures for prevention and control ofcommunicable diseases. We evalueted thestrenghts of the legislation, the weakneses, theopportunities and the threats for the field concern-ing the prevention and control of communicablediseases in Romania, along with measures adoptedwhen Romania became part of the European Union.Results. Results of the evaluation of legislativeframework applicable to transmisible diasease andtheir specific areas for crieteria such as: existence,completeness, compliance with European Unionlegislation or with OMS recomandation, accessibil-ity, coherence, clarity, reveals a generaly adequatelevel of existance, due to the transpostion processof the european provison and for the mainly recom-mendations of international organizations. Someother criteria as coherence, clarity are also met formost of prioritaire transmisible diseases. Somepunctual wekness on clarity and accesibility havebeen identified. The European Integration processrepresents and remains an important opportunity ofexternal enviroment which contributes to the favor-

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able evolution of the regulatory framework fortransmissible diseases. Conclusion. Romania made significant progress inadopting the legislative framework for preventionand control of communicable diseases, especiallyin the last few years, in the context of EuropeanIntegration. The strategy for the future surveillanceof communicable diseases should support furtherimplementation of the existing body of legislation,even completed by some additional standardizedinstruments, in order to contribute to reducing theimpact of this morbidity, on the population health.

21. THE USE OF SOCIAL INDICATORSINSIDE PROGNOSES IN PATIENTS QUALITY OF LIFE. THEORY AND PRACTICEAuthors: Cristina Dobos. Adina Mihailescu,Mihnea Preotesi; The Research Institute for Qualityof Life; Bucharest, Romania

Individual health provides quality of life and it isin close connection with many other dimensionsand aspects of quality of life, like: incomes, area ofactivity, occupation, type of dwelling and comfort,style of life, consumption, leisure possibilities,equity and quality of health and education services,relation with people around, good environment andnot only. In accordance with Health WorldOrganization definitions which were adopted in thelast thirty years human health is not a sickness, butit is a physical, mental and social state of flowering. To evaluate the quality of life of patientes with dif-ferent kind of affections we use some instrumentsof evaluation. The construction of these instru-ments is the theme of our presentation. Frompatients quality of life perspective the most impor-tant beneficiaries of stomatologycal services aretested by evaluated questionnaire to monitorize psi-hological and social impact in progress of daily lifeto stomatologycal affections. Patients are evaluat-ed in relevant aspects of quality of life, in special inthe affection of stomatologycal problems elementslike: food chew, pain, discomfort, sleep, speak,laugh, smil, social relationship, self trust etc.Used instruments for the patient quality of life eval-uation have some reasons:

- to know and keep under supervision psyho-logical and social problems of patient.- to make population inquirys for problemsof health in society.- to evaluate benefits bringing by some treat-ments and health activities for patients.- to make benefits costs analysis

22. COMPARATIVE STUDY BETWEENTHE MEDICINE STUDENTS EXPECTATIONS AND THEIR RESIDENCYCONTEST RESULTSAuthor: Dr. Alin Preda

Introduction: This study aims to highlight the dif-ferences between what the terminal years studentswishes and their activities during the residency pro-gram and to identify the existing discrepanciesbetween these two. Also the study aims to under-line the differences regarding the evaluationmethod changes for entering the residency programand the way this is put into practice.Purpose: development of a reliable tool for moni-toring the progress of the post graduate training ofthe superior medical staffMethods And Materials: The study is descriptiveand retrospective and it integrates in the field of theof the social medicine and human resources devel-opment. The tools used consist in standardized sur-veys for every group of participants. The studypopulation consists in the 5th year students of theCarol Davila Medicine and Pharmacy University ofBucharest and residents of Constanta andBucharest university centers. Other written materi-als from different publications related to the situa-tion of the residents have been used for this study.The analysis of these materials consisted in theessential data extract related to the subject of thestudy, checking of the methods through which thedata have been collected and the synthesis of thedata according to the final purpose of this study.Results: This study offers an overview of theimplications of the residency exam for the futuremedical career.Concusions: The development of a tool designedto measure the residents training results is provedto be an actual must, also for fixing of the imagethat the young graduates should have regardingtheir future career development opportunities andfor creating the post graduate training plan withinthe medical system, this being a necessary step forapproaching the field of “ career management ”.

23. STUDY REGARDING THERESPONSIVENESS OF THE HEALTHCARE SYSTEM IN ROMANIA TO THEEXPECTATIONS OF THE POPULATIONAuthors: Dr Daniela Valceanu, Prof Dr. PetruArmean, Bucharest, Romania

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Responsiveness is a concept that refers to thecapacity of the health care systems, to respond tothe legitimate expectations of the population,regarding non-medical aspects of health care.The aim of this study was to assess the responsive-ness of the health care system in Romania. Materials and methods. The survey regarding theresponsiveness of the Romanian health care systemwas done using the interview with key informants;the questionnaire used was an adapted form of theWHO questionnaire on responsiveness.The key informants were selected from among gov-ernment and non -government employees, of bothsexes and from among a wide range of occupations,from clinicians in hospital settings, to researchers,university lecturers, social workers and staff in theMinistry of Health. The main criterion for inclusionas a key informant was that the person knew thehealth system of the country well.A number of 150 interviews with key informantswere conducted. Results. The respondents considered that:Dignity is usually respected, grading the systembetween 7 and 8. The best scores received weregiven for respectful treatment and respect for theirprivacy. Autonomy is usually respected and the scorereceived is between 7 and 8. Obtaining the patients’consent before starting investigation or treatment,received the highest scores. Confidentiality regarding information about thepatients is usually respected; the score received isbetween 8 and 9. Performing the medical investiga-tion in a confidential manner, maintaining informa-tion confidentiality, maintaining confidentiality ofthe medical act, received the highest scores. Between 50% and 70% of the population benefitfrom prompt access to health services, the scorereceived is 7. Most of the respondents consider thatthey have prompt access to medical and emergencyunits, which are geographically accessible. The patients have always or usually access to socialsupport (represented by family, religious activities,etc.) during the caring period, the score received isbetween 8 and 9. Possibility of having visitors, pos-sibility of friends and family to handle patient’spersonal needs, the possibility to take part in reli-gious activities, received good scores.The quality of basic amenities , with the exceptionof access to a clean water source, was badly scored;the indicator was situated between 5 and 6. Accessto a clean water source received a good score

Patients can usually choose the provider of healthcare, the score received being 8.

24. TRAFFIC INJURIES – PUBLIC HEALTHPROBLEMAuthor: Mihaela Lazar, , UMF “Carol Davila”,Bucharest

Traffic injuries represent a major problem forworldwide public health, being tenth cause ofdecease.Everyday 3000 people dies in worldwide becauseof traffic injuries (OMS), again in European Unionover 40.000 deceases are registering annuallybecause of that. More than that are injured in thisaccidents.

Frequently involved inhabitants in traffic acci-dents are children, pedestrians, cyclists and oldpersons. Deceases occurred in all economiclayers, but frequently in poor population.

Half of deceases caused by traffic accidents involvemen between 15-44 years old, most productivelyinhabitants. In the world, traffic accidents representsecond cause of morbidity and premature decease,after SIDA, to men between 15-44 years old.In this way, European Union has assigned as maintarget, reduce to half of traffic accidents until 2010,involving of factors from health domain in trafficsafety and speed reduce being unmentionables toreach this target.

25. STUDY OF RESIDUAL RIDGE RESORBTION IN THE MAXILLA USINGRADIOLOGICAL INVESTIGATIONAuthors: Stefanescu Corina, Murineanu Rodica;Department of Movable Prosthetics, DentalMedicine Faculty, Ovidius University Constanta.

Objectives: To develop a method for assessingresidual ridge resorbtion in edentulous maxilla andchoose the best prosthetic treatment.Material And Methods: Defined experimental andreference areas in the maxilla were drawn onpanoramic radiographs after scaning. Bone areaswere measured with intergrated planimetry pro-gram.Conclusions: Comparison of the experimental areawith the reference area on same patient panoramicradiographs after 3 or 5 years of prosthetic treat-ment appears suitable for the assessment of residualresorbtion in the maxilla, and gives the opportunityto chose the best treatment.

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Key Words: residual resorbtion, maxilla, panoram-ic radiographs

26. ASPECTS REGARDING THE MANAGEMENT OF OCCLUSION IN THEPROSTHODONTIC TREATMENT OF THETEMPORO-MANDIBULAR DISORDERS Authors: Mihaela Duþã,. Agripina Zaharia, DanielaLaura Rosu, Corina Laura Stefanescu, RodicaMaria Murineanu, Carolina Davidescu., Faculty ofDental Medicine, “Ovidius” University ofConstanta.

Objective: to highlight how quality of the evidencebased dentistry studies is evaluated in dental prac-tice in the temporo-mandibular disorders’ (TMD)prosthetic treatment and to suggest future direc-tions of research in the management of occlusion inthe prosthodontic treatment of the temporo-mandibular disorders.Material and method: a vast search of the MED-LINE database emphasized that many studiesregarding occlusal factors and prosthodontic treat-ment have used the very unspecific diagnosis„TMD”.Results: Evidence-based dentistry that relates toocclusal therapy TMDs proved that unconsideringthe functional occlusion principles has had a nega-tive impact upon oral health.Conclusions: The management of occlusion is asignificant part of the prosthodontic treatment withproper emphasis on patient-centered criteria.Key words: occlusion, temporo-mandibular disor-ders, prosthetic treatment.

27. PREDICTION OF THE ALVEOLARBONE RESORPTION AT THE EDENTULOUS THROUGH THE INVESTIGATION OF THE NUTRITIONALFACTORSAuthors:. Silvia Mihaela Silvaº, University ofMedicine and Pharmacy “Gr. T. Popa” Iasi,Romania

Introduction: The ageing process is a complex,vague phenomenon, incompletly known anddefined. Among the multiple factors which modu-late the process of ageing, nutrition seems to have aconsiderable role. Natural nutritive componentsinfluence this process as far as interacting with thestructural and functional level of genes, by influ-encing the translational events and/or modulating

the post-translational processes (GOOD R.A.,1988). A balanced diet that comprises all the neces-sary nutrients in equilibrate ratios is a crucial factorfor maintaining a good physical condition and forincreasing the quality of life of the elderly. On thecontrary, an insufficient or excessive diet can leadto various degenerative diseases and to the aggra-vation of some modifications associated with theprocess of ageing (MORELY, 1986). One of the problems regarding the diet of the elder-ly is the fact that the aberrant nutritional intake isnot identified in time, by the depletion of the phys-iological processes, in order to correct it promptly.Moreover, recent information regarding the neces-sary nutrient intake for the elderly is based on dataobtained from studies on adults. Aim of study: Thepurpose of the study was to investigate possibleconnections between the daily diet and the resorb-tion of the alveolar crests at the edentate patients.Materials and methods: For the patients includedin the study, we compared the three types of eden-tation (cls. I, II ºi III SANGIUOLO), consideringthe dietary intake of Ca, P and the Ca/P ratio. Wecompared the BMD average values of the lowerjaw bone related to the dietary intake of Ca, for allthe patients included in the study. Results: Theresults obtained indicate a significant connectionbetween the decreased dietray intake of Ca and theresorbtion of the bone (clinically and paraclinical-ly assesed).Key words: diet, nutrients, resorbtion, Ca intake,edentulous

28. THE INCIDENCE OF THE LOCALBONE COMPLICATIONS (THROUGHBONE EXCESS TISSUE) IN ELDERLYEDENTATE PATIENTSAuthors:. Silvia Mihaela Silvaº; University ofMedicine and Pharmacy “Gr. T. Popa” Iasi,Romania

Introduction. The structural and morphologicalcharacteristics of the skeleton in general and themaxillary bones in particular, are considerablymodified by aging. The upper and lower jaws suf-fer an involution characteristic to the other bones ofthe skeleton, but the presence or absence of teethconfers them the particular modifications. The oralchanges occurring at the aged patients, often lead totherapeutic difficulties and complications and mayinterfere with the prosthetics iatrogenesis or theymay impede them. Thus, an important class of

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complications of the prosthetic field is representedby the bone excesses. Aim of study. The aim of the study was to clinical-ly and para-clinically examine the edentatepatients; to assess the local complications deter-mined by bone excess that interfere with the totalprostheses and to establish the incidence of thecomplications among the elderly. Materials and methods. The study comprised 130patients, of which 60 were men and 70 – women,lacking teeth on one or both jaws, with an agerange between 37 and 84 years, with an average of67.4 years. All subjects were thoroughly investigat-ed, both clinically and para clinically. All the datawas centralized and statistically analysed. Conclusions. A rigorous knowledge of the mor-phology of all the elements that form the stomatog-nathic system is necessary for the clinician in orderto evaluate the changes that occur along with dif-ferent forms of prosthetic fields and to take a deci-sion regarding a correct and complex treatment.Therefore, a thorough clinical and paraclinicalinvestigation of the prosthetic field is required inorder to determine the complications and to assessthe causes that may affect the bone tissue of theelderly patient. Key words: edentulous, bone complications, pros-thetic field, protheses.

29. PRELIMINARY STUDY ON HISTOLOGICAL STRUCTURES OFHUMAN ORAL PARAPROSTHETICMUCOUS MEMBRANE AGGRESSED BYFIXED PROSTHESESAuthors: Moldoveanu Lucia Elena, MehedintiTeofil, Draghici Gh.- Faculty of Dental Medicine,“Ovidius” University of Constanta.

The human paraprosthetic gingival mucous mem-brane shows changes generated by the physical andchemical characteristics of dental materials, thedesign of the fixed prosthesis with respect to thetechnological process of manufacturing the fixedprosthesis, as well as the microbial load and thegeneral status of the patient.The study considered patients of both sexes, agedbetween 37 and 73 years, selected according to cri-teria listed in the personal questionnaire and fol-lowing their consent.

The work method consisted in the study ofthe histological preparations obtained by samplingof human paraprosthetic gingival mucous mem-brane after removing the fixed prostheses.

The sampling regarded both the area ofmucous membrane with decubitus lesions clinical-ly presented as well as a section of normal aspect atthe level of the adjacent edentate area.

The histological research of the sampledoral mucous membrane exhibited alterations ofarchitectural complex concerning the structure ofthe gingival mucous membrane with papillomatoushyperplastic epithelium with subjacent chronicinflamed infiltrate and dilated capillaries, exulcer-ated epithelium as well as two cases with hyper-plastic epithelium and corion with granulomatousinfiltrates with nodular arrangement. In conclusion, one can determine correlationsbetween the histological lesion type of the humanparaprosthetic gingival mucous membrane and thepotential etiopathogenic factors

30. POSTEXTRACTION IMPLANT –TREATMENT SOLUTION IN CHRONICDEEP PERIODONTITIS Authors: V. Nicolae, , Mariana Sabãu, Dana-Elena DumitraFaculty of Medicine “ VictorPapilian Sibiu

Aims :In present, chronic deep periodontitis has ahigher frequency at young patients and we consid-er that the treatment must be an alternative to clas-sic methods and must offer several advantages.Matherial and MethodIn order to insert an implant soon after an extractionat patients with chronic deep periodontitis, wemade:1.We collected informations about health status ofthe patients. 2.We made a complex oral examina-tion to find out the cause of periodontitis and thedegree of alteration of the superficial and sustaingperiodontium. 3.Radiologic examination (TC,panoramic radiography) to evaluate the bone offertessential for implant therapy immediately afterextraction.Results and discutions: We madeimplant therapy immediately after extraction at 7cases at the inferior arcade and 7 at superior arcade,in separated meeting therapy. It was also necessarythe augmentation with bone graft protected by aresorbable membrane. In particular, one case with2.5 included, needed odontectomy and augmenta-tion of the alveola. The results were verygood.Conclusions. Using implant therapy immedi-atly after extraction we observed that bone resorb-tion slowed down and our results, functional andestetic, after prosthetic treatment, were special.

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Key words: implant therapy immediatly afterextraction, chronic deep periodontitis.

31.PERIODONTAL PATHOHISTOLOGICALASPECTS AT PATIENTS WITH CHRONICRENAL FAILURE Authors: Mariana Sabãu ,. Vasile Nicolae, DanaDumitra „Lucian Blaga” Universtity, Sibiu

Introduction: Many sistemic diseases may havereactions at the level of oral cavity, so that, it can beconsider a real mirror of the entire body.Haematological diseases, connective deficiencies,diabet mellitus, imun deficiency syndroms, renalfailure, are only a few exemples. Paper purpose:The purpose of these paper is to study the hystolog-ical alterations that apear at the level of gingivaland periodontal structures at patients with chronicrenal failure (CRF), in order to establish the prem-ise of an adequate therapeutical attitude. Matherial and method: In order to obtain theappropriate results we made the following steps: aclinical and a paraclinical study (dental X-rays,blood biology) at patients with CRF, and also ahystologic examination of the gingival papilla.Results and discussions. The cells mantain thecytological caracters of the normal basal layer. Thedisposition is alterated, so that the basal layer ispluristratified. In CRF the cell growth is made bythe cells of the external compartment to the preju-dice of the stem cells. Conclusions. At the patientswith CRF, we observed an increase of the celurari-ty of the basal layer due to acceletarion of themithosis. The intermediar filaments are reduced atpatients with CRF in corelation with the reduceingof the mechanic resistence of the epithelial cells atthe level of the oral mucosa. Key words: periodontal morfology alteration,CRF, therapeutic attitude

32. SUBGINGIVAL MICROBIOTA INROMANIAN PERIODONTITIS PATIENTS Authors: Stela Carmen Hanganu, Alice Murariu;Faculty of Dental Medicine, University ofMedicine and Pharmacy”Gr.T. Popa Iaºi

This study was done in order to compare the preva-lence of 14 subgingival species in 66 periodontalhealthy and 132 adult periodontitis subjects bycheckerboard DNA–DNA hybridization.Subgingival plaque samples were taken from themesial aspect of each tooth in the 198 patients. The

presence and levels of 14 subgingival taxa weredetermined in 3344 plaque samples using the wholegenomic DNA probes and checkerboard DNA-DNA hybridization method.Commonly detected species did not differ signifi-cantly among subject groups. 3 species were signif-icantly elevated and at greater prevalence in peri-odontitis patients than healthy. Mean prevalencefor Porphyromonas gingivalis in healthy and peri-odontitis patients was 5±2, 26±2 (p

investigated. After analyzing the dates we observedthe following clinical aspects: gingival hypertrophywas moderate in 6 cases and light in 3 cases, local-ized at dental papilla, free gum margins, sometimeseven alveolar gingiva, frequently on the buccal sur-face. The specific features for the analyzed pieceswere the following: smooth shiny aspect, soft con-sistency, variable color from red to different shadesof violet, tendency to bleed at contact. All exam-ined patients presented gingival hypertrophy inanterior area, frequently in the incisor-canine lowerarea, only 2 cases in the molar region; in all casesthe gingival hypertrophy is associated with dentalmobility in the incisor-canine region.Key-words: periodontal disease, gingival hypertro-phy, diabetes.

34. EVALUATION OF SALIVAR LACTOFERRIN IN PERIODONTAL DISEASECristina Farcas, Gabriela Lilios, Grigorian Mircea,Lilisa Botea, Panaila Chirea, Victoria Badea, DoinaBalaban, Agripina Zaharia; Physiology Departmentof „Ovidius” University, Faculty of Medicine,Constantza

Periodontal diseases are among the most wide-spread chronic conditions in orodental pathology.Lactoferrin is an iron-binding glycoprotein of the

transferrin family present in human mucosal secre-tions as well as saliva. It is specific neutrophil-derived proteins, an antimicrobial protein, a mark-er for polymorphonuclear leukocytes in inflamma-tion or infection who facilitates in the production ofhydroxyl radicals and binds to iron, thereby imped-ing the growth of microbial organisms. Aim: The present study intended to provide datesabout lactoferrin related with significant changes inperiodontal disease. Material and methods: Lactoferrin concentrationwere determined in unstimulated saliva for 30 vol-unteers with chronic periodontitis. Results: The lactoferrin concentrations were sig-nificantly increased in unstimulated saliva (11,89±0,4µg/ml). Conclusion: The results of this study suggest thatlactoferrin could be a marker for monitoring ofperiodontal disease and changes.

35. HYSTOPATHOLOGICAL STUDY OFHYPERPLASIC GINGIVA IN DIABETESAuthors:Mãdãlina Olteanu, Lelia Gheorghiþã,Victoria Andrei, Mihaela Þuculinã, Faculty ofDental Medicine, U.M.F. Craiova POSTER

Purpose: The study aims to realize a hystopatholo-gyc analysis of tissues obtained by gingivectomyfrom patients with perodontal disease an diabetes.In this study we verified the existence of gingivalhypertrophy in patients with chronic periodontitis;this gingival hypertrophy appears due to hyperpla-sic tissues and not only because of inflamation atthis level.Materials and methods: We studied a number of16 patients presenting diabetes, both type I and typeII, and gingival hypertrophy. There were made 13tooth extractions with gingivectomy and 3 simplegingivectomies. The pieces obtained were kept in a10% formalin solution; the pieces were thenprocessed in order to be included in paraffin. Weobtained sections 3-5 micron thickness which wereprocessed and colored with hematoxyline- eosin. Results: In all cases with gingival hypertrophy wefound a marginal periodontitis, more or less obvi-ous from a clinical point of view, and an unsatisfac-tory oral hygiene. The microscopic exam revealedthat in all cases studied the hypertrophic processinterest the gingival tissue in all layers: epithelium,chorion; the inflammatory process is characterizedby the presence of cellular elements like lympho-cytes and especially plasmocytes; The inflammato-ry process was extended, sometimes it includeseven the apical periodontium.Conclusions: When comparing the histologicalaspects that we have found in patients with gingivalhypertrophy and diabetes with other cases of chron-ic periodontal diseases from the laboratory of his-tology of U.M.F Craiova we could not find specif-ic features from a hystologic point of view.Nevertheless, we could draw an important conclu-sion: in all the cases we studied we cannot talkabout a hypertrophic gingivitis, but a gingivalhypertrophy with an important hyperplasic compo-nent.Key words: hystopathological study, diabetes,hyperplasic gingival.

36. IN VITRO STUDY REGARDING THEAPICAL SEALANT CAPACITY OF SOMERADICULAR OBTURATION MATERIALSAuthors: Radu Mircea Sireteanu Cucui; U.M.FTg.Mures

Introduction. One of the priorities in dental studyis the remand of micro dispersion. The lack of adeterminate method for the objectiveness of quan-titative micro dispersion is probably one of the firstreasons in deficiency of prevention and its

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control.Experimental study. Objective of thestudy: the achievement of the impermeability formicroorganisms and their metabolites. Materialand method: the used material in this study is rep-resented by 15 teeth with straight roots which hadpreviously been cleaned and the access in straightline had been accomplished through the extensionof the cavity; the canals have been conically pre-pared via the step back method; the lateral/verticalcondensation has been practiced on 7 teeth; 1 unob-turated tooth= positive witness. I’ve chosen as dye-ing method via methylene blue. The whole surfaceof the teeth has been covered, except the apicalregion with several layers of coloured nail varnish,sunken in 1% methylene blue solution, PH=6, for 3days at a normal air pressure; washed in runningwater; the insulator layer of coloured nail varnishhas been removed with the help of a lancet. I’vesectioned the teeth longitudinally with a turbinecylinder diamond cutter and an abundant cooling.The sections have been studied with a stereoscopi-cal magnifier (size 9x), photographed (size approx-imately 5x) and the penetration level of the dyealong the canal has been measured in mm, with aprecision of 0,25 mm. The two teeth as negativewitnesses, obturated via lateral and vertical conden-sation, as well as the unobturated positive witnesshave been covered entirely by nail varnish, the api-cal foramen inclusively. Results. In the case of thepositive witness, the dye has penetrated the canal atfull lenght, while in the case of the negative wit-nesses the dye hasn’t penetrated at all. The dye pen-etration has varied between 0.6 mm and 2 mm, withan arithmetic mean of 1.3 mm at lateral condensa-tion and 1.42 mm at vertical condensation, conse-quently a little difference in the favour of the verti-cal condensation. Discussions and inference. Thetime neccessary for vertical condensation (15 min)was much longer than the neccessary lateral con-densation (double), and longer than the one pre-sented in the technical literature. The conclusionsregard the criterias of choosing the material andmethod for a study, the equalization of the resultsregarding the dye penetration, as shown in thetechnical literature.

37. ROOT CANAL MORPHOLOGY OFMAXILLARY FIRST MOLARAuthors: Viorica Teodorescu, Luiza Ungureanu,Anca Nicolae, Marta Gardea Elena Ciobotaru,Faculty of Dentistry, Department of RestorativeDentistry, ‘Ovidius’ University, Constanta.POSTER

Abstract: Root canal morphology of maxillaryfirst molar,

Aim: To investigate the root canal morphology ofmaxillary first molar for 33 teeth using a canalstaining and tooth-clearing technique.Materialand method: Thirty-three extracted maxillary firstmolars were collected from several dental clinics ofConstanta city. Following pulp tissue removal andstaining of the canal systems, the teeth were decal-cified with hydrochloric acid, dehydrated withascending concentrations of alcohol and renderedclear by immersion in methyl salicylate. Clearedteeth were examined by eye and the following fea-tures were evaluated: number and type of rootcanals, presence and location of lateral canals andintercanal communications, location of apicalforamina, and frequency of apical deltas. Results:The majority of the palatal roots had a single canaltype 1 (87.77 %) and only 12.12% had 2 canalstype 4; the distobuccal root had one canal type 1 in96.96% of cases and two canals type 4 in only3.03% of cases; the mesiobuccal root is the mostcomplex of them, it had all types of canals: 3.22%of canals were type 1, 12.90 % type 2, 6.45% type3, 64.51% type 4, 12.90% type 5.Conclusions: Theprevalence of two canals in this group of maxillaryfirst molar was 77% for the mesiobuccal root, fol-lowed by the palatal root, with 12.12% and the dis-tobuccal root with only 3.03%.Keywords: maxillary first molar, morphology, rootcanal.

38. INFLUENCE OF THE EXTERNAL MORPHOLOGY OF THE FIRST UPPERMOLAR IN THE ENDODONTIC TREATMENTAuthors: Viorica Teodorescu, Gabriela Ciobanu,D.F. Petrovici, I. Marin; Department of Endodontics,Faculty of Dental Medicine, “Ovidius” UniversityConstanta. POSTER

Aim: To investigate the extern root canal morphol-ogy of maxillary first molar for 33 teeth using anelectronic device and influence of these proportionsin endodontic treatment. Material and method: Thirty-three extracted max-illary first molars were collected from several den-tal clinics of Constanta city. There were made thefollowing measurements: total length of the teeth,the distance cervical line – furcation, the distanceapex –furcation, buccal-oral diameter for the cervi-

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cal, medium and apical third of the roots, mesio-distal diameter for the cervical, medium and apicalthird of the roots and the following features wereevaluated: length of the palatal root, distobuccalroot and mesiobuccal root; total length of the max-illary first molar, distance cervical line - furcation,buccal-oral diameter and mesio-distal diameter foreach root, apex orientation.Results: The average length of the palatal root was12.40 mm, for the distobuccal root was 11,11mm,and for the mesiobuccal root was 11,64mm. Thetotal average length of the first maxillary molar was18,57mm. The distance cervical line -furcation isbigger mesial and the smallest buccal due to bifur-cation of the roots. The palatal root is flat buccal-oral way, and the mesio-distal diameter is biggerwith 1mm in every third of the palatal root. Themesio-buccal root has the mesio-distal diameterdouble then the buccal-oral diameter. The distal-buccal root is the thinnest root of the first maxillarymolar. The palatal root presents buccal or distobuc-cal apex orientation. The mesiobuccal root presentoften distal apex orientation. The distalbuccal rootpresents mesial apex orientation when is lookingface to face with the mesialbuccal apex or distalapex orientation when the mesiobuccal root isstraight.Conclusions: The palatal root is the longest root,followed by the mesialbuccal root and then the dis-talbuccal root. Knowledge of colet-furcation dis-tance is important during acces to endodonticspace, avoiding accidental procedures. The mesio-distal diameter and the oral-buccal diameter areimportant because the canal space reflects the formof the root. Apex orientation is important for work-ing length determination.

39. THE MODULATE OF DENTAL EDUCATION MESSAGE FOR OPTIMIZATION THE PARAMETERS OFORAL HEALTH

Authors: I.Dãnilã, Iulia Sãveanu, Lucia Bârlean,Carmen Hanganu, Andrei Corneagã, Faculty ofDental Medicine, University of Medicine andPharmacy “Gr. T. Popa” Iasi.

Aim: The aim of this study is evaluation of knowl-edge by dental education of mothers and teachersbecause we consider that mothers, teachers have adefinite influence on the total children population.Materials and methods: The study comprisedmothers and teachers from Iaºi. Data were collect-

ed on the basis of structured questionnaire from200 schoolteachers and 1000 mothers. The SPSS10.1 for windowas statistical software (SPSS Inc2000) was used for data management and statisticaltests. Results and discussions: The study shows asignificant improvement of oral health behaviour,knowledge and attitudes of mothers and school-teach.

40. CORRELATION BETWEEN ATTITUDESAND BEHAVIOR TOWARDS ORALHEALTH OF THE SCHOOLCHILDRENAND THEIR MOTHERS IN BUCHARESTAuthors: Corina-Mona Buzea, Viorica MariaMilicescu, Mihaela Adina Dumitrache, LoredanaCristina Dumitrascu, Faculty of Dentistry,University of Medicine and Pharmacy ”CarolDavila”, Bucharest, Romania.

Abstract: The aim of this study is to emphasize thecorrelation between knowledge, attitudes andbehavior towards oral health of the schoolchildrenand their mothers in Bucharest. Material andmethod: This study was developed on a group of147 schoolchildren of first, third and sixth gradeand their mothers. The subjects responded twotypes of self-administrated WHO questionnairesregarding the knowledge, attitudes and behaviortowards oral health. The schoolchildren question-naire evaluated the knowledge and attitudes towarddiet control, teeth brushing and periodical dentalchecking. Mothers’ questionnaire evaluated theknowledge and attitudes regarding the first perma-nent molar, the etiology of dental caries and its pre-vention and the behavior toward oral health of theirchildren. Data were analyzed using SPSS 10.0 andMicrosoft Office Excel programs. Results and dis-cussions: After applying the two types of question-naires we found certain correlations between theknowledge levels, attitudes and behavior towardsoral health of the schoolchildren and their mothers. Key words: questionnaire, health attitude, oralhealth behavior

41. THE IMPORTANCE OF CLINICALEXAMINATION AT TOTAL EDENTULOUSFOR CHOOSE THE IMPRESSION METHODAuthors: Veronica Mercuþ, Luminiþa Bãtãiosu,Monica Scrieciu, Monica Crãiþoiu, Lucian Dragomir,Monica ªearpe; Faculty of Dental Medicine,University of Medicine and Pharmacy, Craiova,Romania

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Objectives: The examination of the total edentu-lous prosthetic field allow making evident the ele-ments that can contribute to achieve a good sup-port, retention and stability of complete denture, forchoose of impression technique and materials andfor a good prognose about treatment.Materials and method: We had examined 35patients with total edentulous and we had observedthe prosthetic field aspects: buccal mucosa atro-phied edentulous edge and maxillary tuberosity,palatale arch, the buccal retainings at the level ofedentulous edgeand maxillary tuberosity.Following these aspects we formed 3 groups: firstgroup with atrophied field prosthetic, the secondgroup with medium field prosthetic and the thirdgroup with convenient field prosthetic for den-tures.For first group we realized the preliminaryimpression with silicone condensation and finalimpression with bucoplasticcal waxes. For the sec-ond group of patients we took the preliminaryimpression with alginates and the final impressionwith ZOE pastes.At the last group of patients wetook the preliminary impression with alginates andthe second impression with medium silicon.Results: Almost patients was included in secondgroup with medium field prosthetic. The use ofimpression techniques and materials in functionwith prosthetic field quality permited the obtainedof good resultes just in case of difficulte prostheticfield.Conclusions: The achievement of a careful pros-thetic field examination with detect all the positiveand negative elements direct us in choose the bestimpression method.When the prosthetic field isatrophied the quality of the impression material cancompensate that.Key-words: total edentulous, prosthetic field,impression

42. CONSIDERATIONS REGARARDINGTHE POSSIBILITIES OF ESTHETIC REHABILITATION OF THE EDENTULOUSPATIENTAuthors: Crãiþoiu Monica Mihaela, MercuþVeronica, Scrieciu Monica, ªearpe Monica;Prosthodontics Clinic –Faculty of Dentistry, UMFCaiova.

Purpose: Esthetic rehabilitation represents one ofthe major objectives of the prosthodontic treatmentof the edentulous patient. The desire to obtain com-plete dentures that will restore the esthetic function

must be remembered throughout all the stagesrequired for their realization. Material and method: In the ProsthodonticsClinic of Craiova, we have experimented animpression technique and we have named it “theesthetic rehabilitation impression technique”. It hasseveral specific phases with precise objectives. Thepreliminary impression uses the Ivotrays and algi-nate and it is taken at an orientative vertical dimen-sion priorly established. We believe that verticaldimension is the key element that brings togetherall the other elements involved in facial rehabilita-tion. The acrylic custom impression trays are madeusing the Biocop and they have acrylic occlusionrims and spacers. Readjusting the occlusion rimsprovides a new position for the lip, in the intent torehabilitate the nasolabial angle.“The esthetic rehabilitation impression technique”is a mucodynamic technique realized underocclusal pressure and using oxide – eugenol zincpaste and buccoplastic material. One particularthing of this technique is the fact that the impres-sion of the support area is taken before the bordermolding. Border molding is made using buccoplas-tic materials. In an attempt to preview the patient’sfinal appearance, teeth can be placed on the buccalsurface of the occlusion rims.Results: The method has been experimented in thetreatment of 35 edentulous patients and theobtained complete dentures have been proved to bevery stable and to improve facial appearance a lot.Conclusions: This method is suitable for situationsthat demand a very accurate esthetic rehabilitation.

43. QUESTIONNAIRES- EASY METHODSFOR THE EVALUATION OF DENTAL ANXIETY IN TREATMENTS OF ORALREHABILITATIONAuthors: Assist. Prof. PhD. Student. DianaCerghizan, Prof. Dr. Popºor S., Assoc. Prof. Dr.Suciu M. University of Dental Medicine andPharmacy Tg. Mureº, Department of DentalProsthetics and Oral Rehabilitation.

Introduction: Dental Anxiety is common amongthe population. This has resulted; most often avoiddental treatment with serious consequences for oralhealth. Some practitioners tend to skip this aspect,which can lead to trigger anxiety or aggravation.Aim: The purpose of this presentation is to bringattention to practitioners of two scales to measurethe level of anxiety/fear, Modified Dental DentalAnxiety Scale and the Fear Survey, frequently used

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around the world. Methods: The two question-naires have been applied in different locations ofthe city Tg. Mures. Following the establishment ofcriteria for inclusion resulted in a batch of 142 peo-ple which was taken in the study. Findings weresubjected to statistical analysis. Results: Resultsobtained in large part, are similar to the one in lit-erature. Among the questionnaires used haveobtained significant correlation (r = 0.8269).Conclusion: The fact that a large percentage of thelot studied present anxiety over dental treatmentobliges us to draw an alarm signal. Using variousmethods of determining the anxiety / fear is neces-sary in order to give the best possible treatment spe-cialist. Presentation conducted under the projectCNCSIS No TD. 471/9.10.2007, the title of„Research on behavioral factors in the treatment oforal rehabilitation“

44. COMPLETE DENTURES ESTHETICAuthors: Dana Gabriela Budala ,Norina ConsuelaForna, Dan Nicolae Bosinceanu; Faculty of DentalMedicine, University of Medicine and Pharmacy”Gr.T. Popa Iaºi” POSTER

Introduction: In the light of conceptual and techni-cal progress, as well as of the revolution of the math-erials on dental market, the clinician is now con-fronted with a multitude of possibilities of estheticrestaurations. Purpose:This study aimed to establishthe importance of smile in dentures wearers.Matherial and method:60 patients, 23 men and 37women, age between 52-89 years old , were subject-ed to a questionnaire on the image of smile.We pre-sented them photos with denture wearers smile withnatural tooth arrangement, same photos with super-normal arrangements and denture look. Results anddiscussions:A high procent of them 47% choose thenatural tooth arrangements, supernormal was pre-ferred by 13% of the subjects and 40%preferred thedenture look. Conclusions: Within the limitations ofthis study we established that natural arrangementsof tooth was preferred by patients, meaning that thethree concepts of arrangements were differentiatedby patients and that they preffered to look as muchnatural as possible, resembling with the natural den-tition rather than look different from this , even if itwas in a better way.

45. IMPROVING MASTICATORY CAPACITY, ESTHETIC AND PATIENTSATISFACTION WITH IMPLANT-SUPPORTED MANDIBULAROVERDENTURE

Authors: Corina-Marilena Cristache,Bucharest,Romania, Cristache Gheorghe, Private Practice,Bucharest, Romania, Camelia Ionescu, UMF CarolDavila, Dental Medicine, Bucharest, Romania,Daniela Diaconu, Private Practice, Bucharest,Romania. POSTER

The aim of the investigation is to compare in aprospective study the esthetic apparence, satisfac-tion level and masticatory capacity of mandibularedentulous individuals, applying questionnairesadapted from the indexes oral health-related quali-ty of life (OHIP) and its short form OHIP-EDENTduring the phases of rehabilitation treatment with atwo-implants supported overdenture and the use ofStraumann Implant System for elderly patients.Material and Methods: 69 patients (age 42-84)fully mandibular edentulous with severe alveolarridge atrophy and instability of the existing lowerdenture were enrolled in the study.Each patientreceived two screw-type implants in the inter-foraminal region of the mandible. After 6 weekshealing period a new denture was made and thepatients randomly assigned to one of the followingequal groups: retentive anchors(B), magnets(M)and locator system (L). All patients rated with theaid of questionnaires their general satisfaction aswell as other features of their dentures (esthetic,comfort, stability, ability of chewing, speech andcleaning ability) prior to the treatment and at 6 and12 months. Results: All the groups had less oralhealth related quality of life problems than beforetreatment. Conclusions: Rehabilitation withimplants produces a significant improvement in theesthetic apparence, satisfaction level and the masti-catory capacity for elderly patients, despite the factthat the retention force of the magnet attachment issmaller.Acknowledgments: Supported by ITIFoundation for the Promotion of Oral Implantology,Switzerland, Grant no.316/2003 and no.507/207.

46.CLINICAL STUDY OF THE IMPACT ONTHE PROSTETIC TREATMENT OF THEDECREASE OF SALIVARY SECRETION ATOLD PATIENTS Authors: Murineanu Rodica, Stefanescu Corina,Zaharia Agripina, Rosu Laura Duta Mihaela, Da-videscu Carolina, Mobile Prosthetic Departament,Faculty of Dentistry, University Ovidius Constanta.

Purpose: This study analyzes the acute or chronicdecrease of the salivary secretion – xerostomia- atold patients, and the impact on the prosthetic treat-ment.Material and method: A number of 37 patients

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were examined, ages between 58 and 86 years old.We followed the signs at patients with differenttypes of prosthetic treatment. The diagnostic chartwas completed with set of questions with combinedending: close-open.Results: From 37 patients, 29 presented xerostomia.Conclusions: Xerostomia is present both atpatients with general diseases and also at the others.The salivary secretion reduction is very importantat any old patient for the succes of any kind of pros-thetic treatment.Key words: saliva, xerostomia, prosthetic treatment

47. OVERDENTURE ON NATURAL ABUT-MENTS – CLINICAL CASEAuthors. Zaharia Agripina, Rosu Daniela-Laura,.Davidescu Carolina, Vargolici Mihai - DentalProsthetics Department, Faculty of DentalMedicine, “Ovidius” University of Constanta.POSTER

Purpose: Morphofunctional and esthetic restora-tion by improving the maintaining and the stabilityof overall mobile acrylic mandible prothesis.Matherial and methods: The clinic case presentsa male patient 51 years-old choosed from DentalProsthetics Department, Faculty of DentalMedicine, “Ovidius” University of Constanta. Hepresents a mandible under-complete edentationwith two resting teeth canines. After the clinicaland paraclinical exam we decide to preserve thecanine roots and to make an overdenture using spe-cial system attachments. Results: The overdenture on natural abutmentsusing special systems improves the maintaining,stability and the support of mandible prothesisincreasing the patient comfort, decreasing the resid-ual ridge resorbtion and allows the conversion ofprosthetic treatment.Conclusions: The overdenture on natural abut-ments as a treatment method is superior to totalyacrilycal prothesis.Key words: under-complete edentation, attache-ment, overdenture.

48. NEW POSSIBLE STANDARD PARAMETERS FOR PERIODONTAL DISEASE MANAGEMENT THE INTERCELLULAR ADHESION MOLECULE-1 (ICAM-1) LEVELS IN GINGIVAL CREVICULAR FLUID ANDDIRECT MICROSCOPYAuthors: Silviu Alecu, Carmen Defta, AncaDumitriu, Gabriela Bancescu, Silvia Dumitriu,

Faculty of Dental Medicine, University “CarolDavila” Bucharest.Introduction The intercellular adhesion molecule-1 (ICAM-1) is a membrane-bound moleculeinvolved in cell-cell adhesive interactions which isupregulated on epithelial cells in inflammation andcould be important for migration and retention ofinflammatory cells in the damaged tissue. TheICAM-1, also exist in a soluble form (sICAM-1).Purpose We investigated the presence and the rela-tion between the concentration of sICAM-1 in thegingival crevicular fluid and the levels of periodon-topathic bacteria in periodontal pocket frompatients with adult periodontitis.Methods 5 healthysubjects and 14 patients with clinical diagnosis ofadult periodontitis were included in this study.Sampling was performed using steril paper points.The levels of sICAM-1 were studied using anELISA technique (Parameter, R&D systems, USA)and the number and composition of the bacterialflora were observed using direct microscopy tech-nique, with respect to oral spirochetes associatedwith adult periodontitis. The levels of bacterialflora in the sample were labeled from 1 to 4 accord-ing to the number of spirochetes (rare, low, medi-um, high).Results Our method detected sICAM-1values only in 1 from 5 healthy subjects and 6 from14 patients diagnosticated with adult periodontitis.From 7 samples with high levels of sICAM, 6 wereassociated with an increased number of bacteria.sICAM values were correlated with different stagesof plaque accumulation, the higher concentrationthe greater number of bacteria present in the samplewe found. All healthy subjects revealed rare num-ber of spirochetes at direct microscopy. Conclusion These results suggest that elevatedGCF sICAM-1 levels may represent increasedshedding of this molecule in the interstitial fluid asa result of membrane-bound ICAM-1 up-regulationon ICAM-1 gingival-bearing cells in relation withplaque accumulation and inflammation, bothparameter being reflected in the bad clinical status. Undetectable sICAM is associated with decreasedsubgingival plaque. The sICAM-1 values could be use for diagnosisand therapy evaluation in periodontal disease.

49. POTENTIAL CAUSES OF ENDODONTICTREATMENT’S FAILURE OF THIRD MAXILAR MOLARSAuthors: Oana Diaconu1, Marilena Bãtãiosu1,Andreea Didilescu2, Mihaela Þuculinã1, VictoriaAndrei1, Iren Moraru1

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1 Faculty of Dental Medicine, University ofMedicine and Pharmacy, Craiova2 University of Medicine and Pharmacy „CarolDavilla”, Bucharest

Introduction: Third maxilar molars’ morphologyis very complex because of the variety of forms, thenumber of roots and their canals. That is why thirdmolar’s treatment is a veritable challenge for everydentist.Objectives: This study aims to analyze root canalmorphology of third molars using a canal stainingand tooth clearing technique.Material and method: An amount of 38 extractedtooth were studied after colected from dental clin-ics. After pulp tissue’s removal and staining of thecanal systems with China ink, teeth were decalci-f

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