Vanderbilt Research Shared Resources and Core Facilities Susan Meyn Director – Research Resources...

Vanderbilt Research Shared Resources and Core Facilities Susan Meyn Director Research Resources VUMC Office of Research

Agenda Role of the Office of Research VUMC Strategic Plan and Core Facilities VUMC Core Facilities Overview How VUMC Core Facilities can support your Research Efforts VUMC Core Facilities Highlights Resources

ROLE OF THE OFFICE OF RESEARCH

Office of Research: General resources and support for research Point of contact for the research community both on campus and outside the institution Funds and coordinates development of specialized infrastructure (equipment, facilities) to support multiple research efforts Provides grant-writing resources including grant-ready text Coordinates limited submission opportunities and institutional support for selected grant programs contact email: [email protected]@vanderbilt.edu website: http://vanderbilt.edu/oor/http://vanderbilt.edu/oor/

Associate Vice Chancellor for Research Office of Research Shared Resources Oversight Committee Core Advisory Committee Home Center or Department Core Facility Office of Research

Office of Research: Core Facilities and Shared Resources Supports scientific oversight and development of new shared resources, continued development of existing cores Works with research centers and programs to keep core facilities on the cutting edge by providing new equipment, recruiting technical staff. Coordinates with VICTR funding program to provide research support. Oversees and administers the Scholarship program in conjunction with NIH-funded centers to provide assistance with core service fees. contact email: [email protected]@vanderbilt.edu website: http://vanderbilt.edu/oor/http://vanderbilt.edu/oor/

VUMC STRATEGIC PLAN AND CORE FACILITIES

20052010 VANDERBILT UNIVERSITY MEDICAL CENTER RESEARCH ENTERPRISE STRATEGIC PLAN http://www.vanderbilt.edu/oor/about/strategic-plan12-2005 Vanderbilts Research Enterprise Strategic Plan and Core Facilities Personalized Health & Healthcare Therapeutic Discovery & Translation Public Health & Healthcare Foundations of Basic Science Leverages network of shared core facilities in Centers developed through expansion of space, faculty, and $100M investment.

20052010 VANDERBILT UNIVERSITY MEDICAL CENTER RESEARCH ENTERPRISE STRATEGIC PLAN http://www.vanderbilt.edu/oor/about/strategic-plan12-2005 Personalized Health & Healthcare Therapeutic Discovery & Translation Public Health & Healthcare Foundations of Basic Science Leverages network of shared core facilities in Centers developed through expansion of space, faculty, and $100m liquidation of quasi-endowment (2000- 2005). Vanderbilts Research Enterprise Strategic Plan and Core Facilities

Translation Discovery Implementation Personalized Medicine Disease susceptibility Early detection & prevention Variable drug actions Integrative science Disease pathways Drug targets Model systems Individual biomarkers Genomics Proteomics Metabolomics Imaging Research cores/shared resources

VUMC CORE FACILITIES OVERVIEW

Medical Research Building III (MRBIII) Mass Spectrometry Resource Center Neurochemistry Core Laboratory Biomolecular Crystallography Facility Biophysical Instrumentation Core Facility Cell Imaging Shared Resource Medical Center North (MCN) CRCClinical Research Center Division of Animal Care Comparative Pathology Biostatistics Collaboration Center Flow Cytometry and Immunology Cores Medical Center North (MCN) Sleep Research Core Murine Neurobehavioral Laboratory Rat Neurobehavioral Laboratory Vanderbilt University Institute of Imaging Science Relocating soon (VANTAGE): Genome Sciences Resource & BioVU DNA Resources Medical Research Building IV (MRBIV) Transgenic Mouse/ESC Shared Resource VICB Chemical Synthesis Core Cell Imaging Shared Resource Light Hall Cell Imaging Shared Resource Molecular Cell Biology Resource Cores Mouse Metabolic Phenotyping Center ZCORE - Zebrafish Aquatic Facility Robinson Research Building VICB High-Throughput Screening Facility Preston Research Building Cancer Biostatistics Center Epidemiology Biospecimen Core Hormone Assay and Analytical Services Core VICB Protein and Antibody Resource The Vanderbilt Clinic (TVC) Human Tissue Acquisition and Pathology Investigational Drug Services Not shown: Computational Genomics Genetic Studies Ascertainment Survey Research Shared Resource ACCRE - Advanced Computing Center Biomolecular NMR Facility Overview Vanderbilt Institutional Cores

Research core or shared resource: Is a research laboratory accessible to multiple investigators across disciplines. Provides specialized or technologically advanced laboratory services, equipment and expertise. Often based in an NIH funded center, e.g. the Cancer Center. Dedicated personnel include scientific manager/director and technical staff. Supported by chargebacks to investigator grants.

Typical Core Structure Research Center or Department Faculty Core Scientific Director Core Manager or Operations Director Core research technicians Senior Technical Specialists

HOW VUMC CORE FACILITIES CAN SUPPORT YOUR RESEARCH EFFORTS

More than 30 institutional cores and shared resources Whatever your research interest, there is a core facility that can offer high end equipment access, specialized technology or advanced scientific expertise. $1 of every $10 funded by NIH goes to cores at Vanderbilt. Vanderbilt is nationally recognized for the quality and efficiency of its core facilities. Vanderbilt is a leader in core technology development. Scientists at all levels come to Vanderbilt because of our cores make these valuable resources part of your research plan. VUMC Core Facilities support your Research Efforts

Grant applications collaborate with a core: Plan your project and define the scope of services Request core service fees in your budget Do not request personnel costs i.e. no % effort Reviewers are very positive about using core facilities to accomplish research aims. VUMC Core Facilities support your Research Efforts Grant Funding

Experimental design, planning, troubleshooting: Not sure how to start? Take a specific core training session Attend a core seminar Stop by the core to talk with core technical staff Not finding the technology that you need? Contact the Office of Research, or search the core listings. Core facilities help you achieve your research goals. VUMC Core Facilities support your Research Efforts Technical Support

Unfortunately, use of research core facilities is not free! BUT there are sources of funding that can help you pay the bill: Join a research center - for example: Cancer Center Diabetes Center Vision Research Center Digestive Disease Research Center Members of research centers are eligible for core scholarships credit vouchers that can be used to pay core service charges in supported core facilities. Apply for pilot project funding or micro-grant vouchers from VICTR. Investigators may receive up to $2000 every six months to pay for core services. Support to pay core service charges VUMC Core Facilities support your Research Efforts -

VUMC CORE FACILITIES HIGHLIGHTS

Imaging Center for Small Animal Imaging MRI, PET, SPECT, CT, Ultrasound Center for Human Studies MRI and full range of ancillary equipment Cell Imaging Shared Resource Confocal laser scanning microscopes, TEM, image processing workstations

Cell Imaging Shared Resource

Proteomics Mass Spectrometry Resource Center Mass Spec, Proteomics Tissue Imaging Center for Structural Biology NMR, X-ray crystallography, Biophysical instrumentation Institute for Chemical Biology High Throughput Screening and Chemical Synthesis

Olanzapine m/z 313 256 N-desmethyl- m/z 299 256 2-hydroxymethyl- m/z 329 272 Optical image Khatib-Shahidi, S. et al., Anal Chem, 2006, 78, 6448 http://pubs.acs.org/doi/pdf/10.1021/ac060788p in rat, 2 hr post-dose Mass Spectrometry Resource Center

Cell-based Assays, Animal Models Flow Cytometry & Immunology cores cell surface and organelle markers, cytokine assays Transgenic Mouse Embryonic Stem Cell Shared Resource Mouse and Rat Neurobehavior cores

Flow Cytometry Core Figure 2: Distribution of memory, homing, activation and exhaustion markers. Cells from CSF (A) and blood (B) of a HIV+ patient were directly stained with a 14-colour antibody panel. Flow cytometric plots of total CD8+ T cells (in blue) are shown with HIV-specific CD8+ tetramer+ (B7-TL10) T cells on the Y axis (in green) and memory maturation markers (CD45RO, CD62L and CD127), homing markers (CCR5, VLA-4), activation markers (CD38) or markers of T cell exhaustion (PD-1 and CD57) on the X-axis. Percentages on bottom and top of each plot represent frequencies of markers on total CD8+ T cells and HIV-specific T cells respectively. Enhanced PD-1 expression by T cells in cerebrospinal fluid does not reflect functional exhaustion during chronic human immunodeficiency virus type 1 infection. Sadagopal S, Lorey SL, Barnett L, Sutherland D, Basham R, Erdem H, Kalams SA, Haas DW. J Virol. 2010 Jan;84(1):131-40. Epub.

Genomics DNA Resources extract, bank and genotype DNA BioVU - DNA and de-identified clinical databank Genome Sciences Resource microarrays, next-gen sequencing (HiSeq, MiSeq)

RESOURCES

Vanderbilt University Postdoctoral Research and Shared Resources Symposium Wednesday, May 2 nd, 2012 Light Hall North Lobby and Langford Lobby Invited Presentations: 10:30 am - 1:00 pm, LH 208 * Lunch tickets provided for first 50 attendees prior to 10:30 am * 10:30 am Dr. David Piston Louis B. McGavock Chair, Professor of Molecular Physiology & Biophysics, Physics, & Chemical & Biomolecular Engineering 11:00 am Dr. Kevin Schey Director, VVRC Proteomics Core Facility 11:15 amDr. Daniel Foster Vanderbilt Center for Neuroscience Drug Discovery Elucidation of M5-muscarinic receptor neurophysiology in midbrain dopamine neurons using the novel allosteric modulator VU0238429 11:30 am Dr. Christopher Coldren Operations Director, Genomic Sciences Resource 11:45 amDr. Xiaojing Wang Department of Biomedical Informatics customProDB: an R package to generate customized protein database from RNA-Seq data for proteomics search 12:00 pm 15 minute break Lunch provided for first 50 attendees in LH 208 12:15 pm Dr. Sam Wells Director, Cell Imaging Shared Resource 12:30 pm Dr. Thomas Kehl-Fie Department of Pathology, Microbiology & Immunology Manganese sequestration by the host is an essential component of innate immunity 12:45 pm 15 minute break Poster Sessions: 1:00 pm - 3:00 pm, LH Lobby & Langford Lobby * Light refreshments will be provided in Langford Lobby* Session A, LH LobbySession B, Langford Lobby 1:00 - 2:00 pm2:00 - 3:00 pm Animal/Clinical ModelsCell Biology BiochemistryMicrobiology/Immunology Biological SciencesMolecular Physiology & Biophysics Cancer BiologyNeuroscience Pharmacology Keynote Address: 3:30 pm - 4:30 pm, LH 208 Dr. Gregory S. Kopf Associate Vice Chancellor of Research Administration, University of Kansas Medical Center Former Assistant Vice President at Wyeth Pharmaceuticals Weathering the Perfect Storm: Charting the Course for a Career in the Life Sciences Awards Ceremony: 4:30 pm, LH 208 Dr. Richard Peek, 2011 Mentor of the Year Perspectives & Philosophies on Mentoring Presentation of Mentor of the Year Award Presentation of Postdoc of the Year Award Poster awards: Best in Category Best Use of Shared Resources Many thanks to our generous Vanderbilt sponsors! BRET Office of Postdoctoral Affairs & Roger Chalkley Office of Research & Shared Resources Vanderbilt Medical Alumni Association Postdoctoral T32 Training Grant Directors

http://vanderbilt.edu/oor/ Core Listings

http://vanderbilt.edu/oor/ Grant ready text, etc

https://starbrite.vanderbilt.edu/

C.O.R.E.S. Core Ordering and Reporting Enterprise System www.cfuis.mc.vanderbilt.edu Web-interface Place orders directly with core facilities Reserve time on core equipment Review and act on charges made against your grants or VICTR awards

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