Using Iron-rich Fertilizer to increase Hemoglobin in Rhizobium Nodules
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Transcript of Using Iron-rich Fertilizer to increase Hemoglobin in Rhizobium Nodules
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Using Iron-rich Fertilizer to increase Hemoglobin in
Rhizobium Nodules Danny Muslin
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Need
• Approximately 90% of nitrogen that is fixed through natural processes is carried out by free-living bacteria such as Rhizobium
(AG West Biotech)
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Model Organisms
• Organisms used will be:
• Soy Bean Plant
• Rhizobium leguminosarum
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Nitrogen Cycle
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Rhizobium
• Rod shaped Gram -ve bacteria
• Fixes nitrogen after infecting host plants
• Has a mutually beneficial relationship with the host
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Rhizobium Nodules
• Inside the nodules is where nitrogen fixation occurs
• Nodules have a finite lifespan.
(in the case of soybean, estimated at 50-60 days )
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Hemoglobin
• An iron-containing oxygen-transport metalloprotein
Depiction of hemoglobin in a human adult
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Iron Increases hemoglobin Production
• (2008, Ginzburg et al.,) Exogenous iron increases hemoglobin production in beta-thalassemic mice
• (1960, Hammond) Extra iron supplements can be utilized by infants to increase hemoglobin concentration
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Chelated Iron
• Iron that has undergone chelation
• Easier for plants to absorb
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Method of Testing Hemoglobin in Rhizobium Nodules
• Method used will be:
Cyanmethemoglobin Method for Determining Hemoglobin Concentration
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Do-ability
• Can easily obtain soy beans with the leguminosarum rhizobium
• Can easily buy iron chelated fertilizer
• Have a spectrophotometer in the lab