Understanding lethality

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11/21/2012 1 Thermal Process Calculations Overview of the General Method, Ball Formula and NumeriCAL IFTPS SOUTH EAST ASIA TECHNICAL OUTREACH SEMINAR November 27, 2012 Terry L. Heyliger Thermal Processing Manager JBT FoodTech Madera, CA USA Thermal Processing Kill Bacteria in Food by exposing the product/container to HEAT (at a specific temperature) for an specific TIME HEAT HEAT Process Calculations Upon completion of the heat penetration test one must analyze the time / temperature data and use the results of the analysis to calculate a thermal process that will render the product commercially sterile. Process Calculations A critical step in the design of a safe thermal process is the process calculation. It is important that one understands the calculation method employed as well as any conditions that may restrict the use of a particular method. • Sterility (Fo value) The General Method Ball Formula Method Process Calculations • NumeriCALSterility Death of a microorganism is defined as when it has lost the ability to reproduce

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Lethality, Food Safety

Transcript of Understanding lethality

  • 11/21/2012

    1

    Thermal Process CalculationsOverview of the General Method, Ball Formula and NumeriCAL

    IFTPS SOUTH EAST ASIA TECHNICAL OUTREACH SEMINAR

    November 27, 2012

    Terry L. HeyligerThermal Processing Manager

    JBT FoodTechMadera, CA USA

    Thermal Processing

    Kill Bacteria in Food by exposing the product/container to HEAT (at a specific temperature) for an specific TIMETIME

    HEATHEAT

    Process Calculations Upon completion of the heat penetration test

    one must analyze the time / temperature data and use the results of the analysis to calculate a thermal process that will render the product commercially sterile.

    Process Calculations

    A critical step in the design of a safe thermal process is the process calculation. It is important that one understands the calculation method employed as well as any conditions that may restrict the use of a particular method.

    Sterility (Fo value) The General Method Ball Formula Method

    Process Calculations

    NumeriCAL

    Sterility

    Death of a microorganism is defined as when it has lost the ability to reproduce

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    Commercial Sterility

    1. Containers are free of viable microorganisms of public health significance

    MINIMUM PUBLIC HEALTH

    2. Containers are free of microorganisms capable of reproducing under normal conditions of storage and distribution

    COMMERCIALLY STERILE

    12D Concept Minimum Public Health

    Minimum processes are established to reduce any population of the most heat resistant C. botulinumspores by 10-12 or 12 log cycles

    or Reduce the probability of survival to:p y

    1 C. botulinum spore in 1x1012

    or

    1 in 1,000,000,000,000

    5 D Concept Commercially Sterile

    Reduce the population of a common mesophilic sporeformer, C. sporogenes, by 5 log cycles or 10-5

    D-value

    Time in minutes to reduce the population of bacteria by 90% at a specific lethal temperature

    Effect of Temperature on D-value

    As exposure temperature increases the rate of bacterial death also increases

    Effect of Temperature on D-value

    Temperature (F) D-Value 111.1 20.0

    115.5 7.3

    121.1 2.0

    126.1 .63

    131.1 .20

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    z-Value

    Reflects the resistance of bacteria to different lethal temperatures.

    Equal to the number of degrees (F or C) required to change the D-Value by 1 log cycle ( factor of 10)

    Unit of LethalityF-Value

    Sterilization value F expressed as the equivalent minutes at a specified temperature

    Based on the destruction of a population of bacteria having a known z-value

    Unit of LethalityFo-Value

    Represents the equivalent minutes at 121.1oC (250F) at the slowest heating point in the container

    Based on the destruction of a population of bacteria having a z-value of 10C (18oF)

    Lethal Rate Value

    The lethal-rate value assigned to each temperature is numerically equal to the reciprocal of the number of minutes required to destroy the same population of spores in one minute at the reference temperature, 121.1oC

    L = 10 (T-121.1)/10

    Lethal Rate Value

    Temperature, (oC)

    Minutes to Destroy Spores*

    Lethal Rate Value

    101.1 100 0.01

    111.1 10 .1

    121.1 1 1

    131.1 .1 10

    * Same Population of spores with know z-value of 10oC

    Lethal Rate Table