Types of Microscopes
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Transcript of Types of Microscopes
Types of Microscopes
Unit 3 – Powerpoint #1Honors Biology
2012-2013
Use a beam of LightThe specimen can be dead or alive
Light Microscopes
Have only 1 lensGive two examples of a simple microscope:
Simple Microscopes
•Magnifying Glass• Reading Glasses
Simple Microscope
Compound Microscopes
Have more than one ______
lens
Dissecting Microscopes Magnify by 10x or ______ or ________
Shine light on or ____________ the specimen
Specimen can be thick or _______.
20x 30xthrough
thin
Dissecting Microscope
Light Microscope Magnify by ______________
Shines light ______________ the specimen
Specimen must be _________.
40- 400x
thin
through
Light Microscope Image
Electron MicroscopeUse a beam of _______________-The specimen must be _________ because an electron microscope is used in a vacuum with no _____ and no ________
electronsdead
airwater
Scanning E.M.Makes 3D pictures of surfaces
Magnify by _________60,000x
Scanning E.M. Image (3D)
Transmission E.M.
Makes _____ pictures of thin slices
Magnify by __________100,000x
2D
Transmission E.M. Image (2D)
Butterfly Antenna
Probe MicroscopesIon Microscopes◦_____________ can be seen with these 2 types of microscopes.
Other Microscopes
Atoms
Probe Microscope
Probe Microscope Image Horse Hair
Ion Microscopes
Ion Microscope Images
Microscope Parts and Functions
1. Eyepiece
Magnifies by 10x
2. Revolving Nose Piece
Changes Objective
Lens
3. Low power Objective Lens
Magnifies by 4x(Red)
4. Medium Power Objective LensMagnifies by 10x (yellow)
5. High Power Objective LensMagnifies by 40x (Blue)
6. Stage Clips
To hold slide in place
7. Diaphragm
Adjusts the amount of
light
8. Light
Light to see specimen
9. On/Off SwitchTurns light on and off
10. Arm
To hold while
carrying
11. Stage
To set slide on
To focus only when in Low
Power
12. Course Adjustment
13. Fine Adjustment
To focus when using medium or high power
14. Base•To hold while carrying
A. Carry a microscope with ___________ hands – one hand holding the _______
And the other hand under the ___________
Always hold the microscope upright so the _____________ cannot fall out
When you place a microscope at your table, make sure it is _______ from the edge
If there is anything wrong with the microscope, or it is damaged in any way, let the
_______________ know immediately.
B. To clean the objective lenses and eyepiece, use _______________________
Both ArmBase
Eyepiece
Away
Teacher
Lens Tissue
1. Prepare your slide
2. Plug the microscope in. Turn on the ______________
3. Be sure the ___________ Power Objective Lens (_______) is clicked into place.
NOTE: Be sure to look at the microscope from the side when changing objective
lenses to avoid damaging the ________________ and _____________
4. Use coarse focus to raise the stage to its ________________ position
5. Place the _______________ on the stage.
6. Look through the eyepiece and turn the __________________ (lowering the stage)
until the specimen is visible.
7. Use the ____________ adjustment to bring your specimen into sharp focus.
You may wish to move the ________________ to adjust the amount of light.
LightLow 4X
Slide LensHighest
SlideCoarse Focus
FineDiaphragm
8. Make sure the specimen is ______________
9. Switch to the ___________ Power Objective Lens ( ______)
NOTE: Look at the microscope from the ____________ when you change lenses.
10. To avoid losing your focus and/or crushing the slide with the lens, never use the
__________________ focus on medium or high power.
Use the _____________ focus only to make the specimen visible.
11. Make sure the specimen is ______________. Look from the _________ as you
change objective lenses and switch to the __________ Power Lens (________)
12. Use only the _____________ focus to make the specimen visible.
13. When finished, make sure the ___________ power objective lens is in place,
wrap the __________ around the _________ of the microscope, cover, and place
in the appropriate location
FocusedMid 10X
Side
CoarseFine
Focused SideHigh 40X
FineLow
Cord Arm