Review of Microscopes

Plant and animal cell structure

3 types of microscopes

Light Electron Scanning/tunnelling

Types of microscopes

Light microscopes

Always carry with 2 hands Only use lens paper for cleaning Do not force knobs Always store covered Keep objects clear of desk and cords

Microscope Care

EyepieceBody Tube

Revolving NosepieceArm

Objective Lens

StageStage Clips

Coarse FocusFine Focus

Base

DiaphragmLight

Microscope parts

Place the Slide on the Microscope

Use Stage Clips Click Nosepiece to the

lowest (shortest) setting

Look into the Eyepiece Use the Coarse Focus

Using the microscope

Follow steps to focus using low power Click the nosepiece to the longest objective Do NOT use the Coarse Focusing Knob Use the Fine Focus Knob to bring the slide

What can you find on your slide?

Using high power

1. Set up the microscope to view the prepared slides

2. View at least 4 different slides, make a labelled diagram of what you see

3. Classify your cells as being either plant or animal cells

4. Write a simple experimental write up for this experiment

Task 1

Needs the following headingAim: The purpose of the experiment Materials: A list of what was usedProcedure: A step by step description of the processResults: Your labelled diagrams and any other observationsDiscussion: Analysis of results, include similarities and differences between animal and plant cells from your observations, include any errors of difficulties.Conclusion: Write a concluding paragraph about the results and the aim

Write up

Electron Microscopy (TEM) beams of electrons

are used to produce images

wavelength of electron beam is much shorter than light, resulting in much higher resolution with this type of microscope

TEM

The Scanning Electron Microscope (SEM)

uses electrons reflected from the surface of a specimen to create an image

produces a 3-dimensional image of specimen’s surface features

SEM

Resolution