Precipitation · Tube method precipitation Precipitation Reactions: • Involve soluble antigens...
Transcript of Precipitation · Tube method precipitation Precipitation Reactions: • Involve soluble antigens...
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Precipitation
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*Soluble antigen combines with its specific antibody. *Antigen-antibody complex is too large to stay in solution and precipitates.
Done on gel agar media
Precipitation
Single radial immunodiffusion Double Radial Immunodiffusion
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Tube method precipitation
Precipitation
Reactions:
• Involve soluble
antigens with
antibodies
• Ring test (Ascoli
test)
(B. anthracis )
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Single radial
immunodiffusion
Ag
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Single radial
immunodiffusion
][Agr r r α Ag conc.
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single Radial Immunodiffusion
• Interpretation
– Diameter of ring is proportional to the concentration
• Quantitative
– Ig levels
• Method
– Ab in gel
– Ag in a well
Ag Concentration
Dia
met
er2
Ag Ag Ag Ag
Ab in gel
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Double Immunodiffusion (Ouchterlony ) Diffusion of antibody and antigen towards each other in an agarose gel. A line of precipitate will form if the antibody binds to antigen.
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Double Radial Immunodiffusion (Ouchterlony)
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Similar
precipitin lines
Precipitin lines
completely
cross
Precipitin lines
do not form
a complete cross
Ouchterlony test
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Ouchterlony immunodiffusion