True or false? Blood is red because of the iron in hemoglobin.
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True or false? Blood is red because of the iron in hemoglobin. “Red blood cells” A close-up of red blood cells within a capillary. Over 99 percent of the blood vessels in the body are capillaries, even though they only hold 5 percent of the body’s blood.
description
True or false? Blood is red because of the iron in hemoglobin. “ Red blood cells ” A close-up of red blood cells within a capillary. Over 99 percent of the blood vessels in the body are capillaries, even though they only hold 5 percent of the body ’ s blood. False por·phy·rin - PowerPoint PPT Presentation
Transcript of True or false? Blood is red because of the iron in hemoglobin.
True or false?
Blood is red because of the iron in hemoglobin.
“Red blood cells”A close-up of red bloodcells within a capillary.
Over 99 percentof the blood vessels in the
body are capillaries,even though they only hold 5
percent of the body’s blood.
False
por·phy·rinFunction: nounEtymology: from Greek porphyra purple
-any of various compounds with a macrocyclic structure that consists essentially of four pyrrole rings joined by four =CH- groups; especially : one (as chlorophyll or hemoglobin) containing a central metal atom and usually exhibiting biological activity
There are many different hemes used in biochemistry as:
•Electron transfer cofactors as Fe(3+) / Fe(2+)•Catalytic Cofactors as cytochrome P450•Besides O2 transport as hemoglobin
One example:
Cytochrome c Oxidase
Where dioxygen is reduced to water during
respiration
Note all the hemes!
How to draw a porphyrin:
The Adler synthesis of simple, symmetrically substituted porphyrins:
Meso position
R = phenyl (TPP) toluyl (TTP) p-chlorophenyl (TClPP)
Porphyrin substituents on heme in hemoglobin
Metallation of porphyrin
How do you know when the reaction is done?
Anything change at Fe?
Figure 1. Typical UV-Vis absorption spectrum of a porphyrin. The highly conjugated porphyrin macrocycle shows intense absorption (extinction coefficient > 200,000 M-1cm-1) at around 400 nm (the "Soret" band), followed by several weaker absorption bands (Q Bands) at higher wavelengths (450 to 700 nm).
Absorption Spectrum of a Porphyrin
1H NMR Characterization:
Challenges….
and how to meet them
L- keto
N
Ru
NN
N
N
N
N
N
O
NH2
2+
Ru(
L-am
ino)
Ru(
L-ke
to)
Ru(
L-al
lox)
ppm
Complicated spectrum?
Try 2-D COSY NMR
(correlation spectroscopy)
NN
N
N
RuN
N N
N
NH2
a
bc
a'
b'c'
O
NH2
(2)
(1)
from Ben Williams
from Ben Williams
NN
N
N
RuN
N N
N
NH2
a
bc
a'
b'c'
O
NH2
(2)
(1)
from Ben Williams
cʹc1
2
,aʹ
a,bʹ
b
ppm
NN
N
N
RuN
N N
N
NH2
a
bc
a'
b'c'
O
NH2
(2)
(1)
from Ben Williams
from Alex Gaudette. BMC’12
Ru(
L-am
ino)
Ru(
L-ke
to)
Ru(
L-al
lox)
c cʹ 3
d
aʹ
a,b,bʹ
c cʹ
da
,aʹb,bʹ
c cʹ a
,aʹ
b,bʹ
ppm
For Thursday, Sept. 27:•Do heme synthesis using your prophyrin, TPP, TTP or TClPP, determining reaction completion by fluorescence •Filter Ru complex (if necessary); obtain MS of product•Observe 2D COSY NMR process•Obtain UV/vis spectrum of your Fe(porphyrin)Cl complex
Writing Assignments•Goal = writing a full synthesis and characterization manuscript on the Ru complex you made
• Structure like those we discussed on first day• Includes, synthesis, 1D NMR and 2D COSY, and CV
•Due dates
• Draft 1 on Wednesday Oct.3 of the Experimental Section with outline form of introduction
• Draft 2 on Wednesday Oct. 10: Introduction, revised Expermental and Results section with NMR Results
Ru(
L-aa
p)R
u(L-
amin
o)R
u(L-
keto
)
NN
N
N
RuN
N N
N
N
N
a
bc
a'
b'c'
d
cʹc1
2
,aʹ
a,bʹ
b
c cʹ 3
d
aʹ
a,b,bʹ
c cʹ
da
,aʹb,bʹ
Ru(
L-aa
p)R
u(L-
amin
o)R
u(L-
keto
)R
u(L-
allo
x)
NN
N
N
RuN
N N
N
N
N
a
bc
a'
b'c'
d cʹc1
2
,aʹ
a,bʹ
b
c cʹ 3
d
aʹ
a,b,bʹ
c cʹ
da
,aʹb,bʹ
c cʹ a
,aʹ
b,bʹ
![Definition Iron deficiency anemia is caused from a shortness of iron in the body. “[Iron] is a component of hemoglobin (which transports oxygen within.](https://static.fdocuments.us/doc/165x107/56649c9e5503460f9495e8f4/definition-iron-deficiency-anemia-is-caused-from-a-shortness-of-iron-in.jpg)
