Triploid Fecundity: Is it Dad’s Fault?...PowerPoint Presentation Author: Kate M Ritter Created...
Transcript of Triploid Fecundity: Is it Dad’s Fault?...PowerPoint Presentation Author: Kate M Ritter Created...
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Triploid Fecundity: Is it Dad’s Fault?
Kate M. Ritter
Dr. Stan K. Allen
Aquaculture Genetics and Breeding Technology Center
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Recent ABC Research Found that Dying Oysters were Developing Females
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Aren’t Triploids Supposed to be Sterile?
MOST COMMON: No eggs, or very
few eggs
RARE: Prolific eggs
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What Good is a Ripe Triploid?
Essential for tetraploid production
2N
X
= 4N
3N
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4N 4N
4N
Tetraploid Breeding
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Propagating Tetraploids Generation 1
Generation 2
1 million eggs
2 million/ ea.
h2
h2
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Tetraploid Fecundity is Increasing… or is it not?
• Fecundity is variable
• 100,000 – 5,000,000
• Intra-annual variability • Temperature, stress, disease
• Conditioning • System capacity • Algae quality
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Triploid Production
4N 2N
3N
Most of the triploid’s chromosomes come from dad.
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Timeline for Concerns Over Triploid Fecundity
1997 2003 2014 2015 2016
ABC Initiative
First 3Ns in VA
First Fecundity Reports
TMRT
Mortality Project
2018: Fecundity Study
(Hudson and Murray, 2017)
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Triploid Fecundity is Increasing… or is it not?
• Triploids can get ripe
• Is incidence increasing? – Or just the number of farms?
– What role does site, the environment play?
• We can reconstruct the historical record through the pedigree
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Reconstructing the Historical Record
Diploid Dam Group
Number of Families
“HANA” 2
“HANA” 4
“HANA” 4
“HANA” 2
• Is increased fecundity in the tetraploid sire transferring to the triploid offspring? • Used old and new tetraploid lines to create triploids
Theoretical Origination
Number of Generations Bred
Tetraploid Sire Group
2016 1 “LGT”
2014 2 “VBOY”
2006 6 “GNL”
2002 8 “GEN”
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Field Deployment
• Two Sites – Western Shore,
Rappahannock River
– Eastern Shore,
Nandua Creek
• Two reps per family,
per site – Bottom cages
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2018 Sampling: Survival, Sex Ratio, Egg Counts
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Expected Results
0
1000
2000
3000
4000
5000
6000
7000
8000
9000
1 2 3 4 5 6 7 8
Egg
Co
un
t (p
er g
ram
wet
wei
ght)
Generation
?
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Thank you
Kate M. Ritter Flow Cytometry and Lab Specialist, Master’s Student Aquaculture Genetics and Breeding Technology Center [email protected] 804-684-7712 Thank you to Nandua Oyster Company for the use of their farm. Thank you: Beazley Fellowship AAUW IWFA Dr. Eric Guévélou