Thit Aarøe Mørck, LUSH prize 2014

Exposure to environmental chemicals and early markers of adverse effects

- A study using human samples

Thit Aarøe Mørck, PhD student

University of CopenhagenFaculty of Health Science

Department of Public HealthSection of Environmental Health

Outline of today

• Human biomonitoring

• My project

• How I plan to use the LUSH prize money

• Acknowlegdements

Thit Aarøe Mørck, LUSH prize 2014

Human exposure to environmental chemicals

Thit Aarøe Mørck, LUSH prize 2014

Air

Soil

FoodLotions/cosmetics

Toys

Electronics/furnituresWater

For at ændre ”Enhedens navn” og ”Sted og dato”:

Klik i menulinjen, vælg ”Indsæt” > ”Sidehoved / Sidefod”.Indføj ”Sted og dato” i feltet for dato og ”Enhedens navn” i Sidefod

Human exposure with human measurements

Thit Aarøe Mørck, LUSH prize 2014

Chemical exposure

Effects of exposure:Traditional toxicological measurements:- Animal studies- Cell toxicology assays

Biomarkers of exposure-Internal dose

Biomarkers of effect- Early response

Human biomonitoring:

Measurement of total exposure

Thit Aarøe Mørck, LUSH prize 2014

Human biomonitoring

• EU project DEMOCOPHES (DEMOnstration of a study to COordinate and Perform Human biomonitoring on a European Scale)

• 145 mother-child pairs in Denmark

• Two areas (urban and rural)

• Sample collection (urine, hair and blood)

• Questionnaire (mothers)

My project

For at ændre ”Enhedens navn” og ”Sted og dato”:

Klik i menulinjen, vælg ”Indsæt” > ”Sidehoved / Sidefod”.Indføj ”Sted og dato” i feltet for dato og ”Enhedens navn” i Sidefod

64 biomarkers of exposure and effects

Thit Aarøe Mørck, LUSH prize 2014

Phthalates

Parabens PCBs

PFASs

PBDEs

Paracetamol

Phenols

Mercury

Cotinine

Cadmium

Dioxin-like activity

Micronuclei frequency

URINE BLOOD

HAIR

Micronucleus (MN)

• Biomarker of cytogenetic effects

• Association between high exposure to traffic and MN frequency

• Increased risk of cancer development with increased MN frequency

Thit Aarøe Mørck, LUSH prize 2014

Dioxin-like activity

Thit Aarøe Mørck, LUSH prize 2014

Cell assay measuring the hormone receptor (AhR) mediated activity in serum samples

AIM – use of LUSH prize money

• Associations with exposure measurements and early effects

• Patterns of exposure

Thit Aarøe Mørck, LUSH prize 2014

Phthalates

 Parabens

PCBs

PFASs PBDEsParacetamol

Phenols

Mercury

CotinineCadmium Dioxin-like activity

Micronuclei frequency

URINE BLOOD

HAIR

?

Acknowledgements:

Danish Health and Medicines Authority Danish Environmental Protection Agency Danish Veterinary and Food administration

EU projects COPHES and DEMOCOPHES

University of CopenhagenLisbeth E. KnudsenJeanette NielsenJanne Fangel JensenPernille WintonVolkert D. SiersmaLine Mathiesen

Thit Aarøe Mørck, LUSH prize 2014

University of AarhusEva Bonefeld-JørgensenSimon Erdmann

University of Southern DenmarkPhillippe GrandjeanFlemming Nielsen

Free University of BrusselsKim Vande LoockMicheline Kirsch-Volders

LUSH PRIZE ORGANIZATION

For at ændre ”Enhedens navn” og ”Sted og dato”:

Klik i menulinjen, vælg ”Indsæt” > ”Sidehoved / Sidefod”.Indføj ”Sted og dato” i feltet for dato og ”Enhedens navn” i Sidefod

Thit Aarøe Mørck, LUSH prize 2014

Thank you for your attention