The Citric Acid Cycle Mary Gearing. Problems with this pathway Where does the oxaloacetate come...
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Transcript of The Citric Acid Cycle Mary Gearing. Problems with this pathway Where does the oxaloacetate come...
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The Citric Acid Cycle
Mary Gearing
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Problems with this pathway
• Where does the oxaloacetate come from?
• How do we convert oxaloacetate to malate?
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Where in the world is 1.1.1.37?
• BLAST our genome against 1.1.1.37 (H. salinarium)
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Mistaken identity???
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Different BLAST result
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Can our organism convert pyruvate to oxaloacetate? (Enzyme 6.4.1.1)
PyruvatePyruvate
OxaloacetateOxaloacetate
6.4.1.16.4.1.1
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BLASTing our genome
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Both correspond to EC number 2.3.3.13 (2-isopropylmalate synthase)
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So where does the oxaloacetate come from anyway?
• Aspartate metabolism
• Glycoxylate metabolism
L-aspartateL-aspartate OxaloacetateOxaloacetate2.6.1.12.6.1.1
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Unanswered Questions
Oxaloacetate/malate conversion
Pyruvate/oxaloacetate conversion
We still don’t know if these processes occur in H. utahensis.