Team Pegasus MSFSatElysiumCritical Design ReviewJordan Burns, Brenden Hogan, Miranda Link, Cody Spiker, Chris Dehoyos, Hemal SemwalOctober 4th, 2011

Mission Overview• Mission Statement:

• “To test the effects of high altitude flight upon a closed contatiner containing bacterium and recording their response to discover if harsh high altitude effects bacterial reproduction and survival.”

• Objective: • Test and record the effects of a near space environment on several cultures

of Streptococcus mutans. 

• Purpose: • Determine how bacteria cultures respond to the lack of pressure, extreme

temperatures, and radiation present in near-space.

• Why: • Test the validity the of the recorded results of the 1967 flight of Surveyor III.

According to their results, we think that we will find that the bacteria not only survive, but actually thrive in the harsh environment of space. 

Requirements Flow-Down Level 0Level Requirement Description Origin

      

0A Construct a BalloonSat that shall survive an ascent to 30 km

above the surface of Earth and the following decent while maintaining complete functionality. Mission

Statement

B The weight of SatElysium shall not exceed 850 grams, nor a budget of $370.

C SatElysium shall safely transport 6 samples of streptococcus mutans during its flight, studying the effects of temperature and radiation in the stratosphere on the bacteria.

D The streptococcus mutans samples shall be recovered and analyzed post-flight.

E SatElysium shall carry a camera payload to document footage of the flight of the exterior of the satellite.

Level Requirement Description Origin

            

1

A.1 Build a cubical structure that measures 21 cm in height, width, and length out of foam core, hot glue, and aluminum tape. The structure shall contain a rod that shall attach the satellite to the flight string for the duration of the flight.

           

Level 0B.1 The weights of all components shall be monitored during build and the entire satellite

shall be weighed prior to launch.

B.2 Miranda Link shall maintain an updated budget and keep all team members informed of its status.

C.1 Three separate environments shall be created on board the satellite: one that is insulated and heated, one that has no heater, and one that has no heater and is expose to radiation.

C.2 Samples shall be secured to the structure of the satellite with velcro.

C.3 Samples shall contain the bacterium streptococcus mutan.

C.4 Temperature and radiation data shall be collected at regular intervals by a system on board

D.1 Obtain access to a microscope that is of sufficient power to analyze our microbes

D.2 Analyze microbes before and after flight as well as conducting a variety of ground control test.

E.1 Appropriate space for the system in design phases as well as a way for the camera to see out of the satellite.

E.2 Install on board the satellite and program with proper instructions.

Requirements Flow-Down Level 1

Design• Design

• 21 cm cube• 2 levels• Bacteria - 6 different samples, 3 locations with varying

conditions• Radiation• Temperature• Heated and isolated• Ground control (4) samples

• Experiment structure• Petri-dish orientation – tilted for space • Motorized door to expose one sample to the outside environment• Petri-dish support structure

• Analysis of Bacteria (before and after flight)• Colony Count (avg.)• Color• Spores

Design • Microcontroller

• Pressure• Temperature• Light • Hobo Sensors

• Heater (2)• For technical components and (1) bacteria sample

Functional Block Diagrams

Design

Parts:SolderFoam CoreHOBO Arduino ProDigital CameraHeating SystemDivided PetriDishes

9 volt BatteriesAluminum TapeHumidity & Temp. SensorStyrofoamLive Strand of Streptococcus MutansAGARMotorLight Sensor- NOT ORDEREDPressure Sensor – NOT ORDERED

Schedule• 9/12/11: Divide tasks and submit

individual sections by 9/13/11• 9/14/11: Team meeting/Take ITLL Tour

to get access• 9/15/11: Finalize Proposal• 9/16/11: Submit Proposal• 9/19/11: Team meeting for Design

Presentation• 9/20/11: Conceptual Design Review

Presentation• 9/22/11: Team meeting to decide parts

order forms. • 9/27/11: Order satellite hardware• 9/28/11-10/7/11: Build and test

prototypes. Grow first set of bacteria for ground control.

• 10/8/11-10/13/11: Complete testing and design modifications.

• 10/16/11-10/23/11: Construct BalloonSat.

• 10/23/11: Satellite completion• 10/25/11: Pre-Launch Inspection• 10/27/11: In class mission simulation• 11/01/11: Launch readiness review• 11/04/11: Final BalloonSat Weigh in

and turn in• 11/05/11: Launch and Recovery• 11/06/11: Meet to review data• 11/14/11: Review final report• 11/21/11: Complete final report• 11/29/11: Final Team Presentations and

Report• 12/03/11: Design Expo

Test Plan• Our testing will occur in two separate phases.

• The first of these phases will take place during the weekend of October 7-9. • Structural integrity tests

• Whip test• Drop test• Roll test

• The second set of tests will occur after we have all of our materials gathered and ready to perform the necessary experiments.

• The Incubation and Vacuum Chamber • The Cooler test will be the last thing

• need the entire completed BalloonSat ready to run a full-time data collection trial with all components of the working spacecraft in order.

• The software/hardware testing will occur repeatedly throughout our entire building process as things will need to be adjusted every so often.

Testing• Phase I: Structural Testing

• Drop/ Roll Test• Roll down flight of stairs• Drop from a height of 15 meters

• Whip Test• Spinning the Balloonsat by the tether running through it.• Making sure that the balloonsat can withstand the forces acting upon it

during the flight environment.

Different starting orientations to ensure proper testing

Testing cont’d• Phase II: Scientific Materials Testing

• Incubation Test• Temperature, light, and pressure controlled environment to have a sample.• Grown bacteria cultures throughout the semester to analyze a “natural” growth pattern.

• Vacuum Chamber Test• Hand in Hand with the incubation test, merely used as a ground test to show the ground

effects of the bacteria with a “zero pressure” environment

• Software / Hardware Testing• Recursive tests involving the wiring and technical setup of the camera and temperature

sensor arrangement to allow for different capturing intervals of the camera and data collections by the individual sensors that we have.

• Cooler Test• Final test of our satellite. Will ensure that all the technical components will last for the

duration of an entire flight sequence at temperatures exceeding -70 °F.

Expected Results• We expect to observe bacteria response,

• bacterium count, health, reproduction, death and other anomalies

• Compare resulting bacterium tests of both the baseline as well as the space bacteria.• use a microscope• Specifically look at spore count

• We expect to find that the bacteria will be resilient enough to survive in the harsh environment.

• Since our satellite will have three separate environments for testing, there is a real chance of seeing a change between each environment.

BudgetITEM SUPPLIER PRICE Weight

(g) Quantity Total

US flag Gateway Class Provided to us <1 g 1 $0Solder Gateway Class Provided to us <1 g 1 $0

Foam Core Gateway Class Provided to us <100 g 3 $0Hobo Data Logger Gateway Class Provided to us 30 g 1 $0

Digital Camera Gateway Class Provided to us 130 g 1 $0Heating system Gateway Class Provided to us 100 g 1 $0Aluminum Tape Gateway Class Provided to us <3 g 1 $0

9 volt battery Gateway Class Provided to us 150 g 7 $0Arduino Uno SparkFun Electronics $29.95 <5 g 1 $29.95

Arduino Ambient Light Density Cds Photoresistor Sensor

Biozoner $4.50 + $15 S&H 40 g 1 $19.50

Pressure Sensor SparkFun Electronics $15.96 +$2 S&H 1 g 1 $17.96

Humidity & Temp. Sensor SparkFun Electronics $9.95 + $2 S&H <20 g 1 $11.95

Styrofoam McGuckin’s Hardware $3.24 <30g 1 $3.24Live Strand of Streptococcus

mutansWard Science $9.95 + $7.80S&H 1.5 g 1 $17.75

AGAR Scientific Strategies

$40.80 + $22.17S&H

1 $62.97

Stepper Motor Anaheim Automation $14.38 22.7 g 3 $43.14Petri Dish (20) Carolina Biological Supply

Company$8.50 +$17.95 S&H <20 g 1 $26.45

Dry Ice Safeway <$15.00 1 $15.00Total Weight = 699.6 g Total Price = $249.92 incl. S&H

Team OrganizationJordan Burns

Project Manager-responsible for all management

and scheduling-thermal engineer

9118 Andrews Hall, Boulder, CO 80130

(719) 337-5357Jordan.Burns@Colorado.edu

Brenden Hogan-Lead Electrical Engineer

-responsible for circuits and mechanisms

9023 Crosman Hall, Boulder, CO 80310

(303) 483-1161 brenden.hogan@colorado.edu

Miranda Nicole Link-Design and Budget Management

-records of all team expenses, within budget and out of pocket.590 Merlin St., Lafayette, CO

80026(970) 372-8873

Miranda.link@Colorado.eduHemal Sewal-Lead Structural Engineer

-responsible for overseeing the construction of the satellite

9038 AdenHall, Boulder, CO, 80309

(719)-339-7570Hemal.Sewal@Colorado.edu

Cody Spiker-Science Manager

-responsible for all control tests on the bacteria and the growth of bacteria cultures pre-launch

9023 Crosman Hall, Boulder, CO 80310

(970) 589-5689Cody.Spiker@colorado.edu

Christopher Dehoyos-Video Director and Testing Manager

-responsible for designing all tests on the BalloonSat

-oversee extra credit video9130 Darley North Hall, Boulder,

CO 80310(210) 573-8448

Christopher.dehoyos@Colorado.edu

Worries

1. That our petri-dishes may not properly fit in SatElysium.

2. That our insulation system will not properly separate the separate sections into their individual environments that need to be attained.

3. Attaining the proper structural integrity that will allow us to successfully have two separate levels in our SatElysium.