www.lcsciences.com 1-888-528-8818

VariantPro™ Targeted Sequencing LC Sciences offers a customized solution to high-

throughput genome-wide variant analysis.

The VariantPro™ system is powered by a

fundamental improvement in multiplex PCR

technology that solves several issues inherent to

traditional multiplex PCR and delivers high

performance results and cost savings.

Recent advances in next-generation sequencing

(NGS) technologies are providing new

opportunities to characterize individual

genomes and identify causal mutations relevant

for diagnosis and therapy. Though NGS is

capable of very high levels of coverage even on

complex genomes, it is still advantageous to

reduce the complexity of samples and sequence

smaller targeted regions – in particular, when

sample numbers are very high and the goal is

detection of less prevalent or extremely rare

mutations. Targeted sequencing has become

the method of choice for variant analysis due to

more moderate costs, manageable data

amounts and straightforward interpretation of

analysis results.

LC Sciences offers a customized solution to high

-throughput genome-wide variant analysis. The

VariantPro™ system is powered by Relay-

PCR™, a fundamental improvement in multiplex

PCR technology which, when combined with

both the Omega Primer™ and OligoMix®

technologies, effectively overcomes all issues

inherent to traditional multiplex PCR and

delivers high performance results and cost

savings.

INNOVATIVE TECHNOLOGIES Relay-PCR™ - A single PCR run seamlessly

combines two functionally separated reaction

phases, namely target replication and library

amplification in a single tube. This results in a

significantly simplified work flow.

OligoMix® - Custom designed, highly multiplexed

PCR targeted sequencing projects require large

numbers, up to tens of thousands, of custom oligo

primers. OligoMix® massive parallel oligo synthesis

will be available soon for the VariantPro™ system.

Omega Primers™ - A multi-segment design

including two separate binding sections

provides primer design freedoms that balance

priming specificity and binding strength.

LC Sciences has developed new approaches to

perform quantitative prediction of PCR primer

performance and have incorporated the new

approaches into software that performs multi-

plexing PCR primer design.

Single tube, one step workflow

Only 5 minutes of hands-on time required

Online, intuitive design software is coming soon SIMPLE

FLEXIBLE

SCALABLE

RELIABLE

Compatible with Illumina or Ion Torrent technologies

Sample types - fresh frozen, FFPE, genomic DNA

Inputs as low as 10 ng genomic DNA

> 20,000 targets

> 3,000 error correcting barcodes available

Molecular indexing available soon

On-target specificity > 97%

Coverage uniformity > 97%

Reproducible, accurate results

VariantPro™ Targeted Sequencing

Technology

In a single tube, a pair of common primers are added into a mixture of multiple pairs of specific

primers and genomic DNA to form a single step, two stage multiplex PCR reaction.

A single PCR run accomplishes two functionally separated reaction phases, namely target replication

and library amplification, without primer removal step. This results in a significantly simplified work flow.

The addition of the common primers induces a profound change to multiplexing PCR by limiting the role of specific primers to only

the first two thermo cycles for target replication and enabling an automatic switch to common primers in the remaining thermo

cycles for library amplification. This arrangement eliminates a significant cause for amplicon to amplicon variations in end product

due to priming efficiency variations among specific primers which would be exponentially amplified if used as amplification primers.

An Omega shaped primer is composed of three functional sections: a 5’ arm designed

to ensure stable binding to a corresponding template, a 3’ arm designed to double

check sequence specificity and initiate polymerase extension, and a separation

segment between the two arms. This segmented primer design provides desirable features and/or properties for targeted

sequencing applications. The use of two separate binding segments enables:

flexible primer design freedoms such as the balance of priming specificity and binding strength,

reduction in probability of forming amplifiable primer-primer dimmers in highly multiplexed reactions,

primer sections of captured probe sequences remain as short as possible so as to maximize reading length of native sequences.

Genomic DNA Sample

Relay PCR™ Sequencing

RELAY-PCR™

OMEGA PRIMERS™

Phase

1

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specific primer 2

specific primer 1

template DNA

common primer 2

common primer 1

product

cycle 1

cycle 3-n

cycle 2

target specific segments

common primer

common segment

5’ arm 3’ arm

omega

specific primer

tail segment

Phase

2

Lib

rary

Am

plifica

tion

5’ 3’

3’

3’

3’

3’

3’

3’

5’

5’

5’

5’

60-02A-04

www.lcsciences.com 1-888-528-8818

Varia

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rget

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quen

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2575 West Bellfort Street

Suite 270 Houston, TX 77054 USA

Local: (713) 664-7087 Toll Free: 1-888-528-8818

FAX: (713) 664-8181

info@lcsciences.com

Contact Us

HIGH PERFORMANCE

Innovative Relay-PCR™, Omega Primers™ & new approach design software deliver the specificity of PCR and solve the issues with

traditional multiplexing reactions such as primer induced bias.

COST SAVINGS

Higher performance (coverage uniformity & on-target specificity)

translates into sequencing cost savings. Less sequencing depth is required to achieve adequate coverage of all bases.

FFPE SAMPLE OPTIMIZED Our services specifically address customer needs in specimen

types. We have optimized this technology to achieve high performance on difficult specimens such as FFPE samples.

SIMPLE PROCESS / RELIABLE RESULTS

Target selection and library preparation are combined in a single step Relay-PCR™ reaction. A single step workflow means we

deliver fast, accurate results time after time.

COMPLETE FLEXIBILITY

The flexibility of Omega Primers™ enables you to target any

region of any genome. We offer disease specific panels, whole exome capture and custom designed targeted capture services.