Syllabus 3 and 4 Semester

M. S. RAMAIAH INSTITUTE OF TECHNOLOGY

BANGALORE

(Autonomous Institute, Affiliated to VTU)

SYLLABUS

(For the Academic year 2013 – 2014)

III & IV Semester B.E.

BIOTECHNOLOGY ENGINEERING

Dr. S.Y. Kulkarni

Principal

Dr. NVR Naidu

Vice-Principal

Dr. T.V. Suresh Kumar

Registrar (Academics)

Sri. Ramesh Naik

Registrar (Administration)

FACULTY

Faculty Name Qualification Designation Specialization

Dr. Channarayappa MSc (Agri),

Ph.D.(India),

Ph.D. (USA), PDF

(USA), PGDBA

Professor &

Head of the

Department

Biotechnology,

Molecular biology

interdisciplinary

approach,

Agriculture

Dr. Bindu S MSc, Ph.D., PDF

(USA)

Associate

Professor

Food Biotechnology

Toxicology

Dr. Chandraprabha

MN

MSc (Engg.),

Ph.D. (Engg.)

Associate

Professor

Biochemical Engg.

Environmental BT

Dr. Dhamodhar P M.Sc, M.Phil.,

Ph.D.

Assistant

Professor

Immunotechnology

Biochemistry

Mr. Lokesh KN M. Pharm

(Ph.D.)

Assistant

Professor

Pharmaceutical

Biotechnology

Dr. Ahalya N MSc, M.Phil,

Ph.D., PDF

Assistant

Professor

Microbiology

Environmental

Biotechnology

Dr. Sharath R MSc, Ph.D., Assistant

Professor

Plant & Animal BT

Phytochemistry &

Pharmacology

Dr. Ravi Kumar YS MSc, Ph.D., PDF Assistant

Professor

Cancer Biology

Virology

Dr. Harish BG MCA, MSc,

Ph.D.,

Assistant

Professor

Bioinformatics

Plant Biotechnology

Dr. Prabha M MSc, Ph.D., PDF Assistant

Professor

Medical biotechnology

and rDNA Technology

Dr. Sravanti V M.Sc., Ph.D. Assistant

Professor

Genomics & Proteomics,

Structural Biology

Mrs. Samrat K M. Tech Assistant

Professor

Nano-Biotechnology,

Microbial Biotechnology

Mr. Gokulakrishna M M. Tech Assistant

Professor

Bio process Engineering

Ms. Bhavya SG M. Tech Assistant Enzyme Technology

Professor Downstream processing

PROGRAM OUTCOMES

By the time of graduation a Biotechnology Engineering graduate should be able to:

Technical Outcomes

a. Imbibe the essential concepts of both engineering and life sciences & apply it

to a wide range of interdisciplinary work.

b. Understand the engineering design, conduct experiments in biotechnology

and apply in the field by generating innovative, economical and feasible

solutions.

c. Design and automate the processes and programs to accelerate the output

for wide applications

d. Perform and formulate both concept and empirical based equations and

formulas in biotechnology to solve the problems and to draw meaningful

conclusions.

e. Update the modern techniques, skills and advanced engineering tools

essential for applications in biotechnology.

Professional Outcomes

f. Mould the students’ behavior, attitude and interpersonal skills to function in

multi-disciplinary teams and setups.

g. Become a responsible citizen by being aware of his/her roles, duties,

professional and ethical responsibilities and rights.

h. Develop soft-skills through classroom seminars, institutional & industry

interactions, use of modern research and teaching aids

i. Encourage students to take courses from other branches of engineering to

have broad-based education and multidisciplinary approach in a global and

societal context

j. Create enthusiasm in the candidate for life-long learning and urge to

contribute to technology and society by working in a need-based and

problem solving projects.

k. Possess knowledge of contemporary issues for sharpening managerial and

entrepreneurial skills to commercialize the technology & capture the markets

for innovations.

PROGRAM EDUCATIONAL OUTCOME (PEO) ASSESSMENT

The matrix given below describes assessment of PEO defined above against the

outcome a�k as described by ABET (Appendix I)

No

BIOTECHNOLOGY

ENGINEERING PEOs

Program Outcomes (A �� K)

a b c d e f g h i j k

1 Ready to serve a wide

variety of fields that

encompass both

engineering and life

sciences

M M H H H L L L M M M

2 Motivated to pursuing

advanced research in

Biotechnology, Engineering

and related interdisciplinary

fields.

M H H H H M L M

3 Will be capable of

developing highly innovative

research projects that can

help to solve the problems

related to health, food,

environment and

community based.

M H H M M

4 Continue to learn, discover,

develop product and

address the challenges of

national and international.

M M H M

5 Become a very productive

entrepreneur to create job-

opportunities for him-/her-

self and for others.

H M M L M H

CURRICULUM MODEL

This model is developed to encompass the curriculum development processes as given

in Figure 1. It includes periodic curriculum revision. Major steps included in curriculum

revision:

� Students will be assessed periodically for their progress both in academic and

research.

� Research will be oriented towards both Academic and industrial

requirements

� Design of curriculum according to outcome based and end users requirement

� Validation of curriculum effectiveness with feedback from all stakeholders

and observation

� Faculty training and up gradation

Review regularentry students

Review lateralentry students

Introduction ofAudit courses

based on students’background

Foundation coursesIndustry, academia,

research drivenprofessional

electives

Interdisciplinaryopen electives

Developcourses tomatch the

set objectives

Students’performanceindicates are

also set

As the needsof the industrychanges andas the fielddevelopsunique

solutions needto be providedand curriculum

developedaccordingly

STUDENTASSESSMENT

DETERMINECOURSE AREAS

SET GOALS ANDOBJECTIVES OF

COURSES

EVOLVECURRICULUM

VALIDATE ANDIMPLEMENT

Feedback fromindustry experts,

alumni

Periodic reviewby BOS

Adhering toguidelines andregulations by

statutory bodies

Flowchart of core competencies those are pertinent in various fields:

-Unit Operations-Bioprocess principles & Calculations-Cell Biology & Genetics-Molecular Biology-Human Physiology-Immunology-Bio reaction Engineering-Structural Biology-Genetic Engineering-Enzyme Technology-Upstream Process Technology-Bioinformatics-Bioprocess control and Automation-Downstream process technology and bioseparation techniques-Genomics and Proteomics

-Project work

-Maintenence of biotechnology laboratory equipments

-Bio-Separation equipments

-Genetic engineering: specimens & equipments

-Bioinformatics

-Food Biotechnology-Programming in JAVA &C++-Environmental Biotechnology-Process Equipment Design-Fundamentals of OS & SQL-Animal Biotechnology-Microbial Biotechnology-Transport Phenomena-BioPerl & HTML-Fermentation Technology & Design-Agricultural Biotechnology-Biomaterials-Advance Programming using CAD & MAT Lab-Tissue Engineering-Medical Biotechnology-Insilico Drug Design-Nano Biotechnology-Operation Research & Management-Other Institutional Open Elective

-Basic knowldege

-Core competence

-Interdisciplinary learning

-Practical experience

-Research opportunities

-Management

PROFESSIONAL COREPROFESSIONAL

ELECTIVESTECHNOLOGY

CURRICULUMOBJECTIVES JOB PROFILE

-Academics / Teaching

-Research

-Healthcare

-Ennvironmental information systems engineer

-Biomedical engineer

-Product development

-Enterpreneure

-Consultant

-Engineering Physics-Engineering Chemistry-Engineering Mathematics-Biochemistry-Microbiology

-Heat & Mass Transfer-Fundamentals of Computing-Basics of Civil Engineering-Elements of Electronics-Elements of Mechanical Engg.-Basic Electrical Engg.-Engineering Design and Drawing

-Professional communication-English/Kannada-Constitution of India-IPR-Economics & Enterpreneurship-Environmental Studies-Biosafety and Bioethicks

BASIC SCIENCES ENGINEERING SCIENCES HUMANITIES & SOCIAL SCIENCES

SALIENT FEATURES

� Lower semesters will mainly focus more on basic and fundamentals of basic,

engineering courses, to understand the basics necessary for studying applied

sciences.

� Compulsory seminar component has been introduced to prepare students for

scientific data acquisition, interpretation and presentation to develop

professional skills.

� Students will be assessed periodically based on the internal tests,

assignments, practicals, and final examination and project work

presentations.

� Students will be allowed to select their research project with the approval of

their guide(s) and hosting institution.

� Compulsory for UG students to write standard research project report,

publication of paper and presentation of research papers in the national and

international conferences will be highly encouraged.

IMPLEMENTATION STRATEGY

Curriculum Development

Expert Opinion

Approved by Board of Studies

Semester start and end Review with Faculty & Students

Periodic review by BOS and Subject Experts

Feedback from all the stakeholders

CONTINUAL QUALITY IMPROVEMENT

Program outcomes and course learning outcomes will be continuously assessed and

evaluated

• by faculty, subject experts, and students

• giving pertinent information to faculty (lecturers) and Board of

studies/examiners

• on the effectiveness of the design, delivery, and direction of an educational

program.

Improvements base d on periodic re view meeting outcomes and deliberations during

BOS/BOE will close the system loop and the process will continue towards quality

improvement

FOCUSEDEDUCATION

Learningoutcomes

GoodGovernance

Different stakeholders and their interactions contributed for establishment of

outcome based education.

M S RAMAIAH INSTITUTE OF TECHNOLOGY, BANGALORE

(Autonomous Institute, Affiliated to VTU)

SCHEME OF TEACHING FOR THE ACADEMIC YEAR 2013 – 2014

III SEMESTER BE BIOTECHNOLOGY

Sl.

No

Subject

Code Subject Title

Teaching

Dept.

Credits* Contact

hours

Classifi

-

cation L T P Total

1 BTMAT

301

Numerical &

Mathematical

Biology

Mathematics 4 0 0 4

4 BS

2 BT302 Unit

Operations BT 3 0 0 3

3 PSC

3 BT303

Bioprocess

Principles &

Calculations

BT 3 1 0 4

5 PSC

4 BT304 Biochemistry BT 4 0 0 4 4 BS

5 BT305 Microbiology BT 4 0 0 4 4 BS

6 BT306 Cell Biology &

Genetics BT 3 1 0 4

5 PSC

7 BT307L

Unit

Operations

Lab

BT 0 0 1 1

2 PSC

8 BT308L Biochemistry

Lab BT 0 0 1 1

2 PSC

9 BT309L Microbiology

Lab BT 0 0 1 1

2 PSC

Total 21 2 3 26 31

*L – Lecture, T – Tutorial, P- Practicals

IV SEMESTER BE BIOTECHNOLOGY

Sl.

No

Subject

Code Subject Title

Teaching

Dept.

Credits* Contac

t hours

Classifi-

cation L T P Total

1 BTMAT

401

Biostatistics &

Biomodeling

Mathemat

ics 4 0 0 4 4 BS

2 BT402 Heat & Mass

Transfer BT 3 1 0 4 5 PSC

3 BT403 Bioanalytical

Techniques BT 4 0 0 4 4 ES

4 BT404

Biochemical

Thermodynam

ics

BT 3 1 0 4 5 ES

5 BT405 Molecular

Biology BT 3 1 0 4 5 PSC

6 BT406 Human

Physiology BT 3 0 0 3 3 PSC

7 BT407L Heat & Mass

Transfer Lab BT 0 0 1 1 2 PSC

8 BT408L

Bioanalytical

Techniques

Lab

BT 0 0 1 1 2 PSC

9 BT409L Molecular

Biology Lab BT 0 0 1 1 2 PSC

Total 20 3 3 26 32

*L – Lecture, T – Tutorial, P- Practicals

Numerical and Mathematical Biology

Sub Code

Credit

: BT MAT 301

: 4:0:0

CIE

SEE

: 50 Marks

: 50 Marks

Prerequisite: Engineering Mathematics I and II

Course coordinators: Dr. G. Neeraja and Dr. Monika Anand

Objectives:

1. Learn to solve algebraic and transcendental equations numerically.

2. Understand the concept of partial differential equations and it applications to

engineering.

3. Learn the concept of finite differences and its applications.

4. Learn the concepts of fluid dynamics.

Unit I

Numerical solution of Algebraic and Transcendental equations: Method of false

position, Newton - Raphson method.

Partial Differential Equations I: Formation of PDE’S by elimination of arbitrary constants

and arbitrary functions, Solution of PDE - Lagrange’s linear form, Method of separation

of variables.

Unit-II

Partial Differential Equations-II: Derivation of one dimensional heat and wave

equations, Numerical solution of one dimensional heat and wave equations, Two

dimensional Laplace equation, Poisson equation.

Unit III

Finite Differences and Interpolation: Forward and backward differences, Interpolation,

Newton-Gregory forward and backward Interpolation formulae, Lagrange’s

interpolation formula, Newton’s divided difference interpolation formula (no proof).

Numerical Differentiation and Numerical Integration: Derivatives using Newton-

Gregory forward and backward interpolation formulae, Newton-Cote’s quadrature

formula, Trapezoidal Rule, Simpson’s (1/3)rd

rule, Simpson’s(3/8)th

rule.

Unit IV

Models for Blood Flows: Basic concepts of fluid dynamics, Hagen – Poiseuille flow,

Basic concepts about blood, Cardiovascular system and blood flows, Blood flow through

artery with mild stenosis.

Unit V

Models of flows for other Bio-fluids: Peristaltic flows in Bio mechanics, Peristaltic

motion in channels, Characteristic dimensionless parameters. Models for Gas exchange

and air flow in lungs, Alveolar sacs, pulmonary capillaries, Weibel’s model for flows in

lung air ways. Two dimensional flow in renal tubule Function of Renal tube- Basic

equations and boundary conditions.

MATLAB or Mathematica: Tool kits – 2D & 3D Graph Plotting, Data Analysis.

Text Books:

1. B.S. Grewal – Higher Engineering Mathematics – Khanna Publishers – 40th

edition – 2007.

2. J.N. Kapur – Mathematical Models in Biology and Medicine – East-west press

private ltd. New Delhi – 2000.

Reference Books

1. Dennis G. Zill, Michael R. Cullen – Advanced Engineering mathematic –

Jones and Barlett Publishers Inc. – 3rd

edition – 2009.

2. S.S. Sastry – Introductory methods of Numerical Analysis – Prentice Hall of

India – 4th

edition – 2007.

3. B.V. Ramana-Engineering Mathematics-Tata McGrawHill publishing co ltd, New

Delhi – 2008.

Course Delivery: Regular black Board teaching and interaction through tutorial

class

Assessment and Evaluation Vis-à-vis Course outcome

What To

whom

When/

Where

(Frequency in

the course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

ess

me

nt

Me

tho

ds

CIE

Internal

assessme

nt tests Student

s

Thrice(Averag

e of the best

two will be

computed)

30 Blue

books 1,2,3&4

Class- Twice( 10 Assignmen 1,2,3&4

room

open

book

assignme

nt

Average of

the two will

be computed)

t reports

Surprise

Test Once 10

Quiz

answers 1,2,3&4

SE

E Standard

examinati

on

End of course

(Answering 5

of 10

questions)

100 Answer

scripts 1,2,3&4

Ind

ire

ct

Ass

ess

me

nt

Me

tho

ds

End of course

survey

Student

s End of course -

Question-

naire

1,2,3&4,

assessment

methods

Questions for CIE and SEE will be designed to evaluate the various educational

components

SL NO. Bloom’s Category CIE/SEE

1 Remember 20

2 Understand 20

3 Apply 30

4 Analyze 25

5 Evaluate 5

6 Create

Outcome: On completion of this course students are expected to do the following:

1. To solve problems of algebraic transcendental equations numerically.

2. Solve linear partial differential equations.

3. Will be able to use a given data for equal and unequal intervals to find

polynomial function for estimation.

4. Computing maxima, minima, curvature, the radius of curvature using numerical

differentiation.

Mapping of course outcome with program outcomes

Course Outcomes Program Outcome


To solve problems

of algebraic

transcendental

equations

numerically.

X X X

Solve linear partial

differential

equations.

X X X X X X

Will be able to use

a given data for

equal and unequal

intervals to find

polynomial

function for

estimation.

X X X X X X

Computing

maxima, minima,

curvature, the

radius of

curvature using

numerical

differentiation.

X X X X X X

UNIT OPERATIONS

Sub Code

Credit

: BT 302

: 3:0:0

CIE

SEE

: 50 Marks

: 50 Marks


Course coordinators: Mr. M.Gokulakrishnan and Dr.Chandraprabha M N

Objectives: The course will provide:

1. To study dimensional analysis and empirical methods governing the transport

of momentum (fluid flow) in chemical engineering systems.

2. To analyze the application of momentum and energy balances to simple steady

state fluid flow systems and flow through packed beds.

3. To predict the flow measurements and analyze transportation of fluids.

4. To become familiar with techniques to apply these subjects to areas such as

sedimentation, filtration, mixing and size reduction

UNIT-1

Introduction to Fluid Mechanics: Units, Dimensions, Basic and Derived units,

Dimensional homogeneity, Dimensionless numbers, Rayleigh method, Buckingham’s pi

theorem, Similitude. Fluid definition and classification (Types of fluids – Newtonian and

Non Newtonian); Pressure measurement - Manometers.

UNIT- 2

Flow Past Immersed Bodies: Types of flow - laminar and Turbulent; Reynolds number;

Basic equations of fluid flow - Continuity equation and Bernoulli equation; Flow through

circular and non circular conduits – Hagen Poiseuille equation; Flow past immersed

bodies – Kozney-Carmen equation.

UNIT-3

Flow measurements: Flow measurements – Orifice meter, Venturimeter, Rotameter.

Pumps, Centrifugal & Reciprocating pumps, Characteristics of centrifugal pumps.

UNIT-4

Filtration & Agitation and Mixing: Filtration, Types of filtration, Calculation of

resistances and rate of filtration, equipments- plate and frame, Rotary Drum filter

Theory of mixing, Power number calculations, Mixing equipments.

UNIT-5

Flow of Solids through Stagnant Fluids: Settling, Free and Hindered, Stoke’s law,

Newton’s law, Terminal settling velocity, Batch sedimentation theory (Kynch), Thickner

design.

Size Separation & Reduction: Particle shape, size, screen analysis, Size reduction

equipment (Qualitative treatment), Ball mill

Material Handling Equipment: Material handling equipments, Transportation of

materials and storage

Textbooks:

1. McCabe WL, Smith JC and Harriott (2005) Unit operations in Chemical Engineering,

7th

Edn., McGraw-Hill Publications, USA.

2. Gavhane KA (2012) Unit Operations I & II, 22nd

Edn., Nirali Prakashan, India.

Reference Books

1. Badger, Banchero and Walter (1955) Introduction to Chemical Engineering, 3rd

Edn., McGraw-Hill Publications, USA

2. Alan S Foust, Wenzel LA, Clump CW, Maus L, and Anderson LB (2008) Principles of

Unit Operations. 2nd Edn., John Wiley & Sons, USA.

3. Coulson and Richardson’s (2006); Chemical Engineering, Vols I & II., 5th

Edn., Reed

Educational and Professional Publishing Ltd., USA

4. Perry R.H, Green D.W. (2008); Perry’s Chemical Engineering Hand Book, 8th

Edn.,

McGraw-Hill Publications, USA

Course Delivery: Regular black Board teaching and interaction through tutorial class


What To

whom

When/

Where

(Frequency in

the course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

ess

me

nt

Me

tho

ds

CIE

Internal

assessment

tests

Student

s

Thrice(Averag

e of the best

two will be

computed)

30 Blue books 1,2,3&4

Assignmen

t Twice 10

Assignmen

t reports 2&3

Surprise

Test/Quiz Once 10

Blue

books/

Quiz

answer

sheets

1,2&3

SE

E Standard

examinatio

n

End of course

(Answering 5

of 10

questions)

100 Answer

scripts 1,2,3&4

Ind

ire

ct

Ass

ess

m

en

t

Me

tho

ds

End of course

survey Student

s

End of course - Question-

naire ---


components

SL

NO.

Bloom’s

Category

Test 1 Test 2 Test 3 Semester-End Exam

1 Remember 20 20 20 10

2 Understand 25 20 15 20

3 Apply 25 20 15 20

4 Analyze 20 25 30 30

5 Evaluate 10 15 20 20

6 Create 0 0 0 0

Outcome: On completion of this course students will

1. Predict the dimensional analysis and solution for fluid flow problems.

2. Predict the pressure drop in fluid flow and flow through packed beds.

3. Estimate the flow rate of fluids and design the pumps for transportation of

fluids.

4. Analyze and solve the problems on filtration, sedimentation, size reduction and

mixing.


Course

Outcomes

Program Outcome

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

P

O

10

PO

11

PO

12

Predict the

dimensional

analysis and

solution for

fluid flow

X X X X X X X

problems.

Predict the

pressure

drop in fluid

flow and

flow

through

packed

beds.

X X X X

Estimate

the flow

rate of

fluids and

design the

pumps for

transportati

on of fluids.

X X X X X X X X

Analyze and

solve the

problems

on

filtration,

sedimentati

on, size

reduction

and mixing.

X X X X X X X

BIOPROCESS PRINCIPLES AND CALCULATIONS

Sub Code

Credit

: BT 303

: 3:1:0

CIE

SEE

: 50 Marks

: 50 Marks


Course coordinator: Dr.Chandraprabha MN and Mr. M. Gokulakrishnan

Objectives of the course: The course will provide:

1. To correctly implement unit conversions (an ability to apply knowledge of

mathematics, science, and engineering),

2. To analyze and solve elementary material balances on single and multi-unit

process, for both nonreactive and reactive processes

3. To analyze and solve elementary energy balances.

4. To analyze and solve elementary balances for biochemical reactions.

UNIT-1

Introduction & Basic Chemical Calculations: Historical development and overview of

traditional and modern applications of biotechnological processes. Process flow sheet

and unit operations in chemical and bioprocess industries. Fundamental and derived

quantities, Inter-conversion of units from one system to another (FPS, CGS, MKS, SI).

Concept of mole and molecule, Composition of mixtures and solutions- Percentage by

weight, mole and volume; Normality, Molarity, Molality; average molecular weight; ppm,

pH and pK Buffer calculations.

UNIT-2

Material balance without chemical reactions: General material balance equation for

steady and unsteady states. Material balances in Distillation, Absorption, Extraction,

Crystallization, Psychrometry, Drying, Mixing, Evaporation Operations.

UNIT-3

Material balance involving chemical reactions: Principles of Stoichiometry. Definitions

of limiting and excess reactants, fractions and percentage conversion, yield and

percentage yield, selectivity and related problems. Material balances involving bypass,

recycle & purge; Fuels and Combustion: calculations involving Excess air and Air-fuel

ratio.

UNIT-4

Energy Balance: General energy balance equation for steady state. Thermo physics and

Thermo chemistry: Heat capacity, estimation of heat capacity for solids, liquids, gases

and their mixtures. Enthalpy, Standard Heat of formation, standard heat of reaction,

Standard heat of combustion and calorific value, Calculation of ∆(HR) at elevated

temperature. Biochemical equilibrium constants and conversions.

UNIT-5

Stoichiometry of Microbial Growth and Product Formation: Introduction, Definitions of

specific growth rate and yield. Elemental balances and degrees of reduction. Problems

on specific rate and yield.

Textbooks:

1. Hougen OA, Watson KM and Ragatz RA (1996) Chemical Process Principles: Part I,

2nd

Edn., John Wiley, USA.

2. Gavhane KA (2009) Process Calculations Stoichiometry, 22nd

Edn., Nirali Prakashan,

India.

3. Shuler ML and Kargi F (2001) Bioprocess Engineering, 2nd

Edn., Prentice Hall

International, NJ, USA.

Reference Books:

1. David Himmelblau (2003) Basic Principles and Calculations in Chemical Engineering,

7th

Edn., Prentice Hall Inc., USA.

2. Segel IH (1995) Biochemical Calculations 1st

Edn., John Wiley & Sons, NewYork.

3. Bailey JE and Ollis DF (1993) Biochemical Engg. Fundamentals, McGraw Hill,

Newyork, USA.



What To

whom

When/

Where

(Frequency

in the

course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

ess

me

nt

Me

tho

ds

C

I

E

Internal

assessme

nt tests

Student

s

Thrice

(Average of

the best

two will be

computed)


Assignme

nt Once 10

Assignmen

t reports 2,3&4

Surprise

Test/

Tutorial

Once 10 Blue books 3&4

Test

S

EE

Standard

examinat

ion

End of

course

(Answering

5 of 10

questions)

100 Answer

scripts 1,2,3&4

Ass

e

ssm

e

nt End of course

survey

Student

s

End of

course -

Question-

naire ----

CIE and SEE evaluation

S.No Bloom’s Category Test 1 Test 2 Test 3 Semester-End

Examination

1 Remember 10 0 10 10


3 Apply 60 10 20 20

4 Analyze 0 0 0 0

5 Evaluate 0 90 70 60

6 Create 0 0 0 0

Course Outcome: On completion of this course student will have improved ability to:-

1. Acquire knowledge on basic chemical calculations and conduct material

balances around steady- state multi-unit processes without chemical reactions.

2. Conduct material balances around steady- state multi-unit processes with

chemical reactions.

3. Conduct energy balances around multi-unit processes with and without

chemical reactions.

4. Predict stoichiometric requirements of reactants and products in biochemical

reactions.

Mapping of course outcomes with program outcomes

Program Outcome

Course outcome a b c d e f g h i j K

Conduct material

balances around

steady- state multi-

unit processes

without chemical

reactions.

X X X X

X

Conduct material

balances around

steady- state multi-

unit processes with

chemical reactions.

X X X X X

Conduct energy

balances around

multi-unit

processes with and

without chemical

reactions.

X X X X X X

Predict

stoichiometric

requirements of

reactants and

products in

biochemical

reactions.

X X X X X

BIOCHEMISTRY

Sub Code

Credit

: BT 304

: 4:0:0

CIE

SEE

: 50 Marks

: 50 Marks

Prerequisite: Engineering Chemistry

Course coordinator: Dr. P.Dhamodhar & Dr.Ahalya N

Objectives of the course: The course will provide

1. To learn the basic chemical and structural features of important classes of

biomolecules, including peptides, nucleic acids, carbohydrates, and lipids.

2. To develop hands-on laboratory skills in modern biochemical techniques.

3. To develop a sufficient background for those students who wish to study more

advanced biotechnology topics

4. To develop a general foundation for understanding the molecular basis of

human growth, metabolism and disease.

UNIT-1

Introduction to Biomolecules: Carbohydrates, Fats and lipids, Amino acids and Proteins.

Structure, Properties and Classification. Biologically important peptides, Purines,

pyrimidines, nucleotides, Nucleic Acids- DNA and RNA.

UNIT-2

Bioenergetics & Transport Mechanism: Energy, energy flow cycle, Structure and

properties of ATP. High energy compounds, Coupling reactions of ATP and NAD.

Biological membranes: structure, permeability, properties, passive transport and active

transport, facilitated transport, energy requirement, mechanism of Na+ / K+, glucose

and amino acid transport. Organization of transport activity in cell.

UNIT-3

Carbohydrate Metabolism: Glycolysis – Aerobic and anaerobic pathway and energetics,

TCA cycle and its regulation, Calvin Cycle, Glyoxylate cycle, Pentose Phosphate Pathway.

Electron transport chain and oxidative phosphorylation, Gluconeogenesis –regulation of

gluconeogenesis. Biosynthesis of polysaccharides.

UNIT-4

Lipid Metabolism: Biosynthesis of fatty acids, Fatty acid synthase, Biosynthesis of

cholesterol, phospholipids, glycolipids. Biodegradation of fatty acids-beta oxidation

pathway and its energetics.

UNIT-5

Amino Acid & Nucleotide Metabolism: Biosynthesis of amino acids starting from acetyl

CoA (with reference to oxaloacetate family). Biodegradation of amino acids,

deamination, transamination and urea cycle. Biosynthesis, and biodegradation of Purine

& pyrimidine nucleotides: Denova pathway, salvage pathway. Regulation of nucleotide

metabolism.

Textbooks:

1. David L. Nelson, Michel M. Cox (2008) Lehninger Principles of Biochemistry,4th

Edn., Palgrave Macmilan, W H Freeman Publisher, Newyork, USA

2. Jereny M. Berg, John L. Tymoczko, Lubert Stryer (2006) Biochemistry, 6th

Edn. W H

Freeman Publisher, New York, USA.

Reference Books

1. Donal J. Voet, Judith G. Voet, Charlotte W. Pratt (2005) Fundamentals of

Biochemistry, Upgrade Edn. Wiley Publishers, New York, USA.

2. Robert K Murray, Daryl K Granner, Peter A Mayes (2006) Harper’s Illustrated

Biochemistry 27th

Edn. McGraw Hill Book Company, USA.



What To

whom

When/

Where

(Frequency in

the course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

ess

me

nt

Me

tho

ds

CIE

Internal

assessme

nt tests

Student

s

Thrice(Averag

e of the best

two will be

computed)

30 Blue

books 1,2,3&4

Assignme

nt Twice 10

Assignme

nt reports 1&3

Surprise

quiz Once 10

Quiz

answers 1,2&3

SE

E Standard

examinat

ion

End of course

(Answering 5

of 10

questions)

100 Answer

scripts 1,2,3&4

Ind

ire

ct

Ass

ess

me

nt

Me

tho

ds

End of course

survey

Student

s End of course -

Question-

naire -----


components


Examination

1 Remember 30 20 10 20


3 Apply 30 30 30 30

4 Analyze 0 20 20 10

5 Evaluate 0 0 10 10

6 Create 0 0 0 0


1. Classify the macromolecules and analyze their biological and chemical

properties.

2. Differentiate between the biological transport processes and the bioenergetics

accompanied.

3. Analyze and interpret the importance of carbohydrate metabolism in human

body.

4. Understand the importance of Lipid, aminoacid and nucleotide metabolism in

human body.


Course Outcomes

Program Outcome

PO

1

PO

2

PO

3

P

O

4

PO

5

PO

6

PO

7

P

O

8

PO

9

PO

10

PO1

1

PO1

2

Classify the

macromolecules

and analyze their

biological and

chemical

properties.

X X X X X

Differentiate

between the

biological

X X X

transport

processes and

the bioenergetics

accompanied.

Analyze and

interpret the

importance of

carbohydrate

metabolism in

human body.

X X X X X

Understand the

importance of

Lipid, aminoacid

and nucleotide

metabolism in

human body

X X

MICROBIOLOGY

Sub Code

Credits

: BT 305

: 4:0:0

CIE

SEE

: 50 Marks

: 50 Marks

Prerequisite: Nil

Course coordinator: Dr. Ahalya N and Dr. Dhamodhar P

Objectives of the course: This will give knowledge on

1. To understand the different types of microorganisms, their classification,

modes of reproduction and significance.

2. To learn the role of microorganisms in diseases, biofertilisers, food, etc.

3. To evaluate and apply the proper methods of microbial control.

4. To develop hands on laboratory skills in microbiological techniques.

UNIT-1

Introduction: The Scope of Microbiology, History of Microbiology, Microbial diversity

and Taxonomy, Prokaryotes and Eukaryotes, Types of Microorganisms - Bacteria,

Viruses, Fungi, Protozoa, Algae - their Structure, Classification, Modes of Reproduction &

Significance.

UNIT-2

Methods in Microbiology: Basic principles of Microscopy, Bright-Field Microscopy, Dark-

Field Microscopy, Phase-Contrast Microscopy, Fluorescence Microscopy, Electron

Microscopy – SEM, TEM. Pure culture Techniques (streak-plate, spread plate, pour

plate), Cultural characteristics, staining of Microorganisms (Simple staining, differential

staining), types of stains, theories of staining, Enumeration of Microorganisms (Direct

Microscopic Count, by Pour plate technique, Streak plate, membrane filtration,

Electronic Methods, etc), Characterization: Biochemical tests and 16-s rRNA homology

studies.

UNIT-3

Microorganisms- Bacteria: The morphology and fine structure of Bacteria, Cultivation of

Bacteria, Media and types of media Nutritional requirements, Reproduction and

Growth, Factors affecting growth, Measurement of growth. Microbial Metabolism:

Aerobic and anaerobic growth, Metabolic pathways.

UNIT-4

Control of Microorganisms: Control of Microorganisms by physical methods (heat,

filtration, radiation), Control by chemical methods (phenol & phenolic compounds,

Alcohols, Halogens, Dyes, Detergents, Aldehydes, Heavy metals, etc), Antibiotics and

other chemotherapeutic agents.

UNIT-5

Applications of Microbiology: Microbiology of soil, Biofertilizers, Microbes in

Bioremediation, Traditional microbial processes using yeasts and bacteria, Microbes as

source of protein, Microbial Insecticides, Enzymes from microbes, primary and

secondary metabolites. Clinical Microbiology. Pathogenesis of microorganisms, Human

diseases caused by microbes viruses (HIV), bacteria (TB, Cholera and E.coli), Protozoans

(Malaria and amebiasis).

Textbooks:

1. Prescott LM, Harley JP, Klein DA (2002) Microbiology, 5th

Edn. McGraw- Hill

Publications, USA

2. Pelczar MJ, Chan ECS, Kreig NR (1993) Microbiology, 5th

Edn. Tata Mc-Graw Hill,

India.

3. Channarayappa (2006) Molecular Biotechnology: Principles and Practices,

Universities Press (India) Pvt. Ltd., CRC Press Worldwide.

Reference Books

1. Channarayappa (2010) Cell Biology: Universities Press (India) Pvt Ltd.

2. Black J (2008) Microbiology: Principles and Explorations, 6th

Edn. John Wiley and

Sons,USA,

3. Tortora GJ, Funke BR, and Case CL (2006) Microbiology: An Introduction, 4th

Edn.,

Benjamin-Cummings Pub Co, USA.

4. Ingraham JA, Ingraham CA (2004) Introduction to Microbiology, 3rd

Edn. Int

Thomson Computer Press, USA

5. Cappuccino J.G, Sherman N (1999) Microbiology: A Laboratory Manual, 4th

Edn.,

Addison-Wesley International Student.

6. Prescott, Harley and Klein (2008) Laboratory Exercises in Microbiology, 7th Ed

Harley, McGraw-Hill, USA

7. Pollack RA, Walter F, Mondschein W, Modesto R (2004) Laboratory Exercises in

Microbiology, 2nd

Edn.John Wiley Publication.

Course Delivery: Regular black Board teaching and power point presentations

What To

whom

When/

Where

(Frequency in

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

the course)

Dir

ect

Ass

ess

me

nt

Me

tho

ds

CIE

Internal

assessm

ent tests

Student

s

Thrice(Averag

e of the best

two will be

computed)

30 Blue

books 1,2,3&4

Class-

room

open

book

assignm

ent

Twice(

Average of

the two will

be computed)

10 Assignmen

t reports 1,2,3&4

Surprise

quiz Once 10

Quiz

answers 1,2,3&4

SE

E

Standar

d

examina

tion

End of course

(Answering 5

of 10

questions)

100 Answer

scripts 1,2,3&4

Ind

ire

ct

Ass

ess

me

nt

Me

tho

ds

End of course

survey

Student

s End of course -

Question-

naire

1,2,3&4,

Assessment

methods


components


Examination

1 Remember 40 40 30 30


3 Apply 25 15 30 25

4 Analyze 05 05 05 10


6 Create 0 0 0 0

Course Outcome: On completion of this course student will have improved ability to-

1. Explain the core principles of microbiology.

2. Differentiate among different categories of microbes

3. Perform aseptic technique and demonstrate appropriate laboratory safety

skills for working with microbes and mastery of basic microscopic staining

techniques required for visualization of microbes.

4. Understand the relationship between microbes and biotechnology, including

environmental, industrial, and medical applications


Course

Outcomes

Program Outcome

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

P

O

10

P

O

11

P

O

12

Explain the

core

principles of

microbiology.

X X X X X

Differentiate

among

different

categories of

microbes

X X X

Perform

aseptic

technique

and

demonstrate

appropriate

laboratory

safety skills

for working

with

microbes and

mastery of

basic

microscopic

X X X X X

staining

techniques

required for

visualization

of microbes.

Understand

the

relationship

between

microbes and

biotechnolog

y, including

environment

al, industrial,

and medical

applications

X X X X X

CELL BIOLOGY AND GENETICS

Sub Code

Credit

: BT 306

: 3:1:0

CIE

SEE

: 50 Marks

: 50 Marks

Prerequisite: Nil

Course coordinator: DR. Ravi kumar Y. S. and DR. Prabha M.

Objectives of the course: This will give the Knowledge on:

1) The structural and functional unit of life: The Cell-evolution, organization of

plasma membrane, cytoplasm and other organelles.

2) The study of cell cycle phases and cell division, cell signaling with different

types of receptors and secondary messengers.

3) The basic background about the genetics: Mendelian’s principles, genes

behavior, alleles and types of chromosomes and disorders.

4) The subject to understand the random changes in genetic material: Mutation,

the causes and its types with population genetics and benefits of Hardy-

Weinberg principle.

UNIT-1

Cell Structure & Function: Introduction and scope of cell biology; Evolution of

prokaryotic & eukaryotic cells; Structural details. Plasma membrane: structural

composition, organization and functions. Cytoskeleton – eukaryotic & prokaryotic;

eukaryotic cytoskeletal proteins – types, structural organization and functions. Cell-Cell

junctions.

UNIT-2

Cell Organelles: Structural organization and functions of cell organelles: Nucleus,

Mitochondria, Chloroplast, Endoplasmic reticulum, Golgi bodies, Ribosomes, Lysosomes,

Peroxisomes, Vacuoles.

UNIT-3

Cell Cycle & Cell Signaling: Cell cycle; Mitosis and Meiosis. Introduction to cell signaling-

para, endo and autocrine; different Receptors: cell surface, ion channel linked;

neurotransmitter, G protein linked; beta adrenergic receptor, cAMP and enzyme linked;

Ras-DAG, calmodulin.

UNIT-4

Mendelian Principles and Heredity: Introduction & scope of Genetics; Mendel’s

principles. Gene interaction and linkage; Multiple alleles– blood groups and Rh factor.

Chromosome: Fine structure of chromosome-solenoid model; Euchromatin and

heterochromatin; Sex determination in plants & animals; Sex chromosomes in human;

Sex linked disease- hemophilia; Autosomal linked disease-color blindness; Sex limited

and sex influenced traits. Problems-- on Mendelian genetics, gene interactions, multiple

alleles; sex linked inheritance.

UNIT-5

Mutation and Population Genetics: Mutation- types of mutations; chemical, physical

and transposon as mutagens; significance of mutation. Chromosomal aberrations-

Structural & Numerical. Point mutations & frame shift mutations. Population genetics -

Hardy-Weinberg principle closed and open populations; allele, genotype and genotype

frequencies; MN blood group. Problems-Hardy-Weinberg principle.

Textbooks:


2. Eldon John Gardner et al (2003), Principles of Genetics, VIII Edn. John Wiley & Sons,

Singapore.

3. Susan Elord & William Stansfield (2005) Genetics, V Edition, Tata-McGraw-Hill

Publishing Co.Ltd., West Patel Nagar, New Delhi.

Reference Books

1. Bruce Alberts et al (2008) Molecular Biology of the Cell, V Edition, Taylor & Francis

Group, New York

2. William Stanfield et al (2005) Molecular Biology & Cell Biology, Tata-McGraw-Hill

Publishing Co.Ltd., West Patel Nagar, New Delhi

3. Freifelder D (2002) Molecular Biology, ,III Edition, Narosa Publishing House, Madras.

4. Gupta PK (2005) Cell & Molecular Biology, III Edn. Rastogi Publications, Meerut.

5. Lodish et. al. (2003) Molecular Cell biology, V Edition, W.H. Freeman & company,

New York.

6. Benjamin Lewin (2006) Gene IX by, Jones & Bartlett Publishers, Singapore.



What To

whom

When/

Where

(Frequency in

the course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

ess

me

nt

Me

tho

ds

CIE

Internal

assessme

nt tests

Student

s

Thrice(Averag

e of the best

two will be

computed)

30 Blue

books 1,2,3, & 4

Class-

room

open

book

assignme

nt

Twice(

Average of

the two will

be computed)

10 Assignmen

t reports 1,2,3, & 4

Case

analysis Once 6

Case

solutions 1,2,3, & 4

Surprise

quiz Once 4

Quiz

answers 1,2,3, & 4

SE

E Standard

examinat

ion

End of course

(Answering 5

of 10

questions)

100 Answer

scripts 1,2,3, & 4

Ind

ire

ct

Ass

ess

me

n

t M

eth

od

s

End of course

survey

Student

s End of course -

Question-

naire

1,2,3, & 4

Assement

methods


components

SL NO. Bloom’s Category Semester-End Exam

1 Remember 20

2 Understand 20

3 Apply 35

4 Analyze 20

5 Evaluate 5

6 Create 0

Course Outcome: On completion of this course student will have improved ability:-

1) To learn the importance of the cell, its origin, the organelle structure and

function.

2) To get the basic information to understand the steps behind the cell division,

signal transduction with messengers and receptors.

3) To gain the knowledge of phenotypic and genotypic characters of genes and

reasons behind chromosomal disorders.

4) To know the information in the significance and functions of mutations and

Hardy-Weinberg principle for population genetics.


Course Outcomes

Program Outcome


To learn the importance of

the cell, its origin, the

organelle structure and

X X X X

To get the basic

information to understand

the steps behind the cell

division, signal

transduction with

X X X X X

To gain the knowledge of

phenotypic and genotypic

characters of genes and

reasons behind

chromosomal disorders.

X X X X X X

To know the information in

the significance and

functions of mutations and

Hardy-Weinberg principle

for population genetics.

X X X X X X

UNIT OPERATIONS LAB

Sub Code

Credit

: BT307L

: 0:0:1

CIE

SEE

: 50 Marks

: 50 Marks

Prerequisite: Unit operations

Course coordinator: Mr. Samrat K and Mr. Gokulakrishnan M

Objectives of the lab: This will give the Knowledge on:

1. To measure the flow rate of the fluids using variable head meters.

2. To calculate the frictional losses in pipes.

3. To separate the particles based on size.

4. To calculate the area of filtration and sedimentation equipments.

LABORATORY

LIST OF EXPERIMENTS

1. Friction in circular pipes.

2. Flow rate measurement using orificemeter

3. Flow rate measurement using Venturimeter

4. Flow through annulus.

5. Study of pump Characteristics.

6. Flow through helical coil.

7. Losses across joints & fittings

8. Sieve analysis.

9. Determination of screen effectiveness.

10. Determination of minimum thickener area using Batch sedimentation tests.

11. Constant pressure / constant rate filtration using leaf filter.

12. Verification of Stoke’s law in Free/Hindered Settling.

13. Size reduction using Ball mill

14. Size reduction using crusher

Note: Any 12 experiments must be performed

Reference Books



2. Alan S Foust, Wenzel LA, Clump CW, Maus L, and Anderson LB (2008) Principles of

Unit Operations. 2nd Edn., John Wiley & Sons, USA.

3. Coulson and Richardson’s (2006); Chemical Engineering, Vols I & II., 5th

Edn., Reed

Educational and Professional Publishing Ltd., USA

Course Delivery: Regular black board teaching and interaction through Laboratory

sessions


What To

whom

When/

Where

(Frequency

in the

course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

ess

me

nt

Me

tho

ds CIE

Internal

assessment

tests

Studen

ts

Once 30 Blue books 1,2,3&4

Lab

Assesment Continuous 10

Attendanc

e 1,2,3&4

Record Continuous 10 Record 1,2,3&4

SE

E Standard

examinatio

n

End of

course 50

Answer

scripts 1,2,3&4

Ind

ire

ct

Ass

ess

me

n

t M

eth

od

s

End of course

survey

Studen

ts

End of

course -

Question-

naire ---


components

SL NO. Bloom’s Category Lab Exam SEE

1 Remember 20 20

2 Understand 20 20

3 Apply 20 20

4 Analyze 30 30

5 Evaluate 10 10

6 Create 0 0


1. Calculate the pressure drop through circular conduits, pipes and fittings and

annulus.

2. Estimate the flow rate using variable head meters and study the pump

characteristics.

3. Analyze the particle size and predict the surface area of the particles.

4. Predict the settling regime and calculate the thickener area.


BIOCHEMISTRY LAB

Sub Code

Credit

: BT308L

: 0:0:1

CIE

SEE

: 50 Marks

: 50 Marks

Prerequisite: Engineering chemistry

Course coordinator: Dr. Sharath, R. and Dr. P.Dhamodhar


1. To learn fundamental approaches for experimentally investigating biochemical

problems

2. Understand the applicability of the biochemical methods to realistic situations.

3. To enhance the practical approaches in estimation of carbohydrates, proteins

and lipids

LABORATORY

Course

Outcomes

Program Outcome

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

P

O

10

P

O

11

PO

12

Calculate the

pressure drop

through

circular

conduits,

pipes and

fittings and

annulus.

X X X X

Estimate the

flow rate

using variable

head meters

and study the

pump

characteristics

.

X X X X X X X

Analyze the

particle size

and predict

the surface

area of the

particles.

X X

X X X

Predict the

settling

regime and

calculate the

thickener

area.

X X

LIST OF EXPERIMENTS:

1. Qualitative tests for Carbohydrates

2. Qualitative tests for Amino acids and Proteins.

3. Titration of Amino acids- Sorenson’s formal titration.

4. Estimation of phenol by FC method.

5. Estimation of reducing Sugar by O-Toludine / DNS method.

6. Estimation of inorganic Phosphate by Fiske-Subbarao method.

7. Estimation of Amino acid by Ninhydrin method.

8. Estimation of Protein by Lowry’s method/Biuret method

9. Estimation of Urea by Diacetyl Monoxime method.

10. Estimation of Iron by Wong’s method.

11. Determination of Saponification value of Lipids.

12. Determination of Iodine value of Lipid.

13. Determination of Peroxide value.

14. Determination of total carbohydrate by Anthrone method


Reference Books

1. Sadasivam S, Manickam A (2005) Biochemical Methods. Revised Second

Edition, New Age International Pvt. (L.)Publisher.

2. Artinigam, ArchanaAyyagari(2008). Lab Manual in Biochemistry,

Immunology and Biotechnology, Tata Mac Graw Hill- Publishing

company Ltd.


sessions


What To

whom

When/

Where

(Frequency

in the

course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

ess

me

n

t M

eth

od

s

CIE

Internal

assessme

nt tests Student

s

Once 30 Blue

books 1,2,3&4

Lab Continuous 10 Attendanc 1,2,3&4

Assesmen

t

e


SE

E Standard

examinati

on

End of

course 50

Answer

scripts 1,2,3&4

Ind

ire

ct

Ass

ess

me

nt

Me

tho

ds

End of course

survey

Student

s

End of

course -

Question-

naire

1,2,3&4 and

assessment

methods


components


1 Remember 10

2 Understand 10

3 Apply 10

4 Analyze 30

5 Evaluate 40

6 Create


1. To understand the experimental methods used in the biochemistry research

laboratory

2. To acquaint with the properties of biomolecules such as amino acids, peptides,

proteins, lipids, and carbohydrates.

3. Understand the applicability of the biochemical methods to realistic situations.

4. To enhance the practical approaches in estimation of carbohydrates, proteins

and lipids


Course

objective

Program Outcome

PO

1

PO2

b

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

P

O

P

O

10 11

To understand

the

experimental

methods used

in the

biochemistry

research

laboratory

X X X

To acquaint

with the

properties of

biomolecules

such as amino

acids,

peptides,

proteins,

lipids, and

carbohydrates

.

X X X

Understand

the

applicability of

the

biochemical

methods to

realistic

situations.

X X X

To enhance

the practical

approaches in

estimation of

carbohydrates

, proteins and

lipid

X X

MICROBIOLOGY LAB

Sub Code

Credit

: BT309L

: 0:0:1

CIE

SEE

: 50 Marks

: 50 Marks

Prerequisite: Microbiology

Course coordinator: Dr. Ahalya N and Dr. Dhamodhar P


1. Provide students with an understanding of important facts, concepts, and the

investigative procedures of a microbiology laboratory.

2. Train students in aseptic technique, and the proper methods relating to the

safe maintenance of microorganism.

3. Train students in fundamental laboratory methodology to include the use of

differential media, and associated reagents.

4. Provide students with a hands-on familiarity with basic research procedure for

identification of unknown microorganismal specimens.

LIST OF EXPERIMENTS

1. Media preparation, plugging and sterilization.

2. Microscopic observation of Prokaryotic and Eukaryotic cells (bacteria, fungi,

yeasts and moulds).

3. Hanging drop experiment to study motility.

4. Enumeration of total count (haemocytometer) and viable count of bacteria.

5. Identification of bacteria by size, shape, Gram reaction.

6. Isolation of bacteria by serial dilution, pour plate and streak plate techniques.

7. Biochemical tests- IMViC tests, Starch hydrolysis, Catalase activity.

8. Isolation, culturing and identification of microorganisms from air.

9. Isolation, culturing and identification of microorganisms from water.

10. Isolation, culturing and identification of microorganisms from soil.

11. Antibiotic susceptibility testing of a bacterium.

12. Alcoholic and mixed acid fermentation.(Wine, Lactic acid).

13. Growth curve of bacteria (Demonstration).

14. Endospore staining.


Reference Books

1. Prescott LM, Harley JP, Klein DA (2002) Microbiology, 5th

Edn. McGraw- Hill

2. Cappuccino J.G, Sherman N (1999) Microbiology: A Laboratory Manual, 4th

Edn., Addison-Wesley International Student.

3. Prescott, Harley and Klein (2008) Laboratory Exercises in Microbiology, 7th Ed

Harley, McGraw-Hill, USA


sessions


What To

whom

When/

Where

(Frequency

in the

course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

ess

me

nt

Me

tho

ds

CIE

Internal

assessment

tests

Student

s

Once 30 Blue

books 1,2,3&4

Lab

Assessment Continuous 10

Attendanc

e 1,2,3&4


SE

E Standard

examination

End of

course 50

Answer

scripts 1,2,3&4

Ind

ire

ct

Ass

ess

me

nt

Me

tho

ds

End of course

survey

Student

s

End of

course -

Question-

naire

1,2,3&4 and

assessment

methods

Questions for CIE and SEE will be designed to evaluate the various

educational components

ASSESSMENT PATTERN:

SL NO. Bloom’s Category Lab Internal test Semester-End

Exam

1 Remember 10 10

2 Understand 10 10

3 Apply 20 10

4 Analyze 30 30

5 Evaluate 30 40

6 Create 0 0

Marks distribution:

CIE: 50 Laboratory internal tests will be conducted 30 marks. Lab

Assessment/Record will be conducted 20 marks.


1. Operate equipment used in microbiology laboratory.

2. Identify microorganisms using morphological and physiological tests.

3. Prepare growth media and isolate microorganisms from various sources

4. Perform aseptic transfers.


Course outcome

Program Outcome

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

Operate

equipment used

in microbiology

laboratory

X X X X X

Identify

microorganisms

using

morphological

and physiological

tests.

X X

Prepare growth

media and isolate

X X

microorganisms

from various

sources

Perform aseptic

transfers

X X X

BIOSTATISTICS AND BIO MODELING

Sub Code

Credit

: BTMAT401

: 4:0:0

CIE

SEE

: 50 Marks

: 50 Marks

Prerequisite: Numerical and mathematical biology

Course coordinator: Dr. Monika Anand & Dr. G. Neeraja


1. Study of Biostatistics, curve fitting and correlation and regression.

2. Learn the concept of basic probability and random variables.

3. Learn different probability distributions and stochastic process.

4. Study of genetic applications of probability.

Unit I

Statistics and Probability: Scope of Biostatistics, Correlation and regression analysis

(Simple and Linear) Curve fitting (Linear, Parabolic and Geometric curves)

Probability: Classical definition, Axioms, Addition rule, Multiplication rule, Conditional

probability, Baye’s theorem.

Unit II

Random variables and Probability distributions: Random variables: Discrete and

continuous random variables, Mean and variance, Binomial distribution, Poisson

distribution, Geometric distribution, Exponential distribution, Uniform distribution,

Normal distribution.Stochastic Process : Classification, Unique fixed probability vector,

Regular stochastic matrix, Transition probability matrix, Markov chain.

Unit III

Genetic application of probability and Statistical inference: Genetic Applications of

Probability, Hardy - Weinberg law, Estimation of probabilities for multi-locus/multi-

allele finger print systems. Sampling and Statistical inference : Sampling Distributions,

Standard error, Central limit theorem, Testing of Hypothesis, Level of significance,

Confidence limits, One tailed and two tailed tests, Test of significance for small samples,

t- distribution, F distribution and Chi-square distribution.

Unit IV

ANOVA and Optmization models: Analysis of variance (One way and Two-way

classifications) : Case studies of statistical designs of biological experiments (RCBD and

RBD), Single and double – blind experiments, Limitations of experiments. Optimization

models in Biology and Medicine – Medical diagnosis problem, Hospital diet problem.

Unit V

Biomodeling: Microbial growth in a chemostat, Growth equations of microbial

populations, Models of commensalisms, Mutualism, Predation and Mutation. Lotka -

Volterra’s model for n Interacting species. Basic models for inheritance, Selection and

Mutation models, Genetic inbreeding models – Selfing, Sipmating

Text Books:

1. Marcello Pagano and Kimberlee Gauvreu – Principles of Biostatistics –

Thompson Learning – 2nd

edition – 2004.

2. Ronald N. Forthofer, Eun Sui Lee – Introduction to Biostatistics – Academic

press – 2006.

3. J. N. Kapur – Mathematical Models in Biology and Medicine- East-West Press

Private Ltd. – New Delhi – 2000.

Reference Books:

1. Warren J. Ewens, Gregory R. Grant – Statistical methods in Bioinformatics –

Springer publications – 2nd

edition – 2006.

2. P. S. S. Sundar Rao and J. Richard – An Intrduction to Biostatistics- Prentice Hall

of India – 4th

edition – 2006.

3. Wayne W. Daneil – Biostatistics: A foundation for Analysis in the Health

sciences – John Wiley & Sons – 7th

edition – 2000.



What To

whom

When/

Where

(Frequency

in the

course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

ess

me

nt

Me

tho

ds

CIE

Internal

assessm

ent tests

Student

s

Thrice(Avera

ge of the

best two will

be

computed)

30 Blue

books 1,2,3&4

Class-

room

open

book

assignme

nt

Once 10 Assignme

nt reports 1,2,3&4

Surprise

Test Once 10

Blue

books 1,2,3&4

SE

E Standard

examinat

ion

End of course

(Answering 5

of 10

questions)

100 Answer

scripts 1,2,3&4

Ind

ire

ct

Ass

ess

me

nt

Me

tho

ds

End of course

survey

Student

s End of course -

Question-

naire

1,2,3&4,

Assesment

methods


components


1 Remember 20

2 Understand 20

3 Apply 30

4 Analyze 25

5 Evaluate 5

6 Create


1. Fit a suitable curve for the tabulated data by the method of least squares.

2. Predict the probable characteristics possessed by the off springs of nth

generations.

3. Apply different types of tests to test the hypothesis relating to small samples.

4. Modeling of growth of micro organisms in a chemostat.

Mapping of course outcomes with program outcome

Course Outcomes

Program Outcome


Fit a suitable curve

for the tabulated

data by the method

of least squares

X X X

Predict the

probable

characteristics

possessed by the off

springs of nth

generations

X X X X

Apply different

types of tests to

test the hypothesis

relating to small

samples.

X X X X X X X

Modeling of growth

of micro organisms

in a chemostat

X X X X X

HEAT & MASS TRANSFER

Sub code

Credit

: BT 402

: 3:1:0

CIE

SEE

: 50 Marks

: 50 Marks

Prerequisite: Unit operations

Course coordinator: Mr. Samrat K and Dr Chandraprabha MN


1. To study the modes of heat transfer and thermo-physical properties and

application of energy conservation equation for thermal problems and heat

flux in one and dimensional conduction.

2. Evaluate heat transfer rate and to calculate heat transfer area and

effectiveness of different heat exchangers.

3. To study the various modes of mass transfer, to determine mass transfer rates

and to estimate diffusion coefficients.

4. To study principles of distillation, absorption drying and extraction operations.

UNIT-1

Introduction to Heat Transfer: Modes of heat transfer; Conduction – steady state heat

conduction through uni-layer and multilayer plane wall sphere, cylinder; Insulation –

types, critical radius, Optimum thickness of insulation. Forced and Natural convection;

Significance of Dimensionless numbers (Nu, Gr, Pr, Re, Pe numbers only); Heat transfer

without phase change, heat transfer in laminar and turbulent flow inside closed

conducts, concepts of film heat transfer coefficients.

UNIT-2

Heat Transfer Equipments: Equations and numerical problem for calculations of film

heat transfer coefficients, Heat transfer with phase change - Condensation – film wise

and drop wise; Boiling – types of boiling. Co current and counter current flow. Individual

and overall Heat transfer coefficients, LMTD, Elementary design of double pipe heat

exchanger and shell and tube heat exchanger.

UNIT-3

Basics of Mass Transfer: Diffusion - Fick’s law of diffusion. Measurement of diffusivity,

Mass transfer coefficients and their correlations. Two film theory, Individual and Overall

Mass Transfer Co-efficients.

Distillation: Vapour liquid equilibrium, T-xy, P-xy, x-y equilibrium diagram, Raoults Law,

Azeotropic mixures, steam distillation. Numerical problems.

UNIT-4

Mass Transfer operations: Distillation –Methods of distillation –Simple, Flash distillation

of binary mixtures – relative volatility, fractionation of binary mixtures -McCabe Thiele

method, Extractive and Azeotropic distillation, numericals.

UNIT-5

Other Mass Transfer operations: Drying, Drying rate, Drying curve and calculations,

Principles of: Extraction, Adsorption, and Absorption, Typical equipments.

Textbooks:

1. McCabe WL, Smith JC and Harriott (2005) Unit operations in Chemical Engineering,

7th


2. Treybal RE (2012) Mass Transfer Operations, 3rd

Edition, McGraw-Hill Publications,

USA.

3. Gavhane KA (2011) Unit Operations I & II, 25th

Edn., Nirali Prakashan, India.

Reference Books


Edn.,

McGraw-Hill Publications, USA .

2. Alan S Foust, Wenzel LA, Clump CW, Maus L and Anderson LB (2008) Principles of

Unit Operations, 2nd Edn., John Wiley & Sons, USA.

3. Coulson and Richardson’s (2006) Chemical Engineering, Vols I & II, 5th

Edn., Reed

Educational and Professional Publishing Ltd., USA.

4. Kern (2001) Process Heat Transfer, 2nd

Edn., McGraw-Hill Publications, USA.

5. Perry RH and Green DW (2008) Perry’s Chemical Engineering Hand Book, 8th

Edn.,

McGraw-Hill Publications.



What To

whom

When/

Where

(Frequency

in the

course)

Max

mark

s

Evidence

collected

Contributin

g to Course

Outcomes

Dir

ect

Ass

ess

me

nt

Me

tho

ds

CIE

Internal

assessment

tests Studen

ts

Thrice(Avera

ge of the

best two will

be

computed)

30 Blue

books 1,2,3&4

Assignment Once 10 Assignme

nt reports 1,2,3&4

Surprise/Tut

orial Test Once 10

Blue

books 1,2,3&4

SE

E

Standard

examination

End of

course

(Answering 5

of 10

questions)

100 Answer

scripts 1,2,3&4

Ass

e

ssm

e

nt End of course

survey

Studen

ts

End of

course -

Question-

naire ----


components

SL

NO.

Bloom’s

Category

Test 1 Test 2 Test 3 Semester-End Exam

1 Remember 20 20 20 10


3 Apply 25 20 15 20

4 Analyze 20 25 30 30

5 Evaluate 10 15 20 20

6 Create 0 0 0 0


1. To determine the temperature and heat flux distribution using energy

conservation and/or Fourier heat law and to determine the heat flux and

temperature distribution in steady state one-dimensional problems using

thermal resistance concept.

2. To determine the heat flux in turbulent flows using empirical equations

and to estimate the heat transfer rate for different heat exchangers.

3. To determine mass transfer rates and mass transfer coefficients and

relation between individual and overall mass transfer coefficients.

4. To determine various parameters of mass transfer operations.

Mapping of course outcomes with program outcome

Course

Outcomes

Program Outcome

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO1

0

PO1

1

PO1

2

To

determine

the

X X X X X X X

temperatu

re and

heat flux

distributio

n using

energy

conservati

on and/or

Fourier

heat law

and to

determine

the heat

flux and

temperatu

re

distributio

n in

steady

state one-

dimension

al

problems

using

thermal

resistance

concept.

To

determine

the heat

flux in

turbulent

flows using

empirical

equations

and to

estimate

the heat

transfer

rate for

X X X X X X X

different

heat

exchangers.

To

determine

mass

transfer

rates and

mass

transfer

coefficient

s and

relation

between

individual

and

overall

mass

transfer

coefficient

s.

X X X X X X

To

determine

various

parameter

s of mass

transfer

operations

.

X X X X

BIOANALYTICAL TECHNIQUES

Sub code

Credits

: BT 403

: 4:0:0

CIE

SEE

: 50 Marks

: 50 Marks

Prerequisite: Engineering Physics & Engineering Chemistry

Course coordinator: Dr Ahalya N and Dr Sravanti G


1. A systematic and comprehensive understanding of the principles of modern

bioanalytical techniques

2. The ability to critically evaluate methodologies and experimental bioanalytical

data

3. Exposure to principles, instrumentation & application of various instruments &

techniques used in biological field.

4. Ability to choose an appropriate analytical approach for specific problem

solving.

UNIT-1

Introduction: Types & Basic concepts of analytical methods, instruments for analysis,

electromagnetic radiation - its properties and interaction with matter. Emission of

radiation. Preparation of standard solutions and buffers, pH and Oxygen electrodes. An

introduction to absorption spectroscopy, photometry, beer lamberts law.

UNIT-2

Spectroscopy: UV, visible, IR spectrophotometry theory and instrumentation,

Turbidometry & Nephelometry: Principles & Applications, Spectrofluorimetry: Principles

& Applications. Flame Emission and Atomic Absorption Spectroscopy: Principles &

Instrumentation. NMR Spectroscopy: Principles, Instrumentation, Applications Raman

Spectroscopy: Principles, Instrumentation, Applications

UNIT-3

Mass Determination:. Mass Spectrometry: Principles, Instrumentation, and

applications. Centrifugation – Principles of centrifugation, concepts of RCF, different

types of instruments and rotors, preparative, differential and density gradient

centrifugation, analytical ultra-centrifugation, determination of molecular weights and

other applications, subcellular fractionation.

UNIT-4

Chromatography: Distribution coefficient, modes of chromatography like Paper, Thin

Layer, Molecular Exclusion chromatography: Principles, Instrumentation, Applications,

Ion Exchange and Affinity chromatography – Principles & Applications Gas liquid

chromatography: Principles, Instrumentation, Applications. High Pressure Liquid

Chromatography (HPLC): Principles, Instrumentation, Applications.

UNIT-5

Electrophoresis: General principles, Support media, Electrophoresis of proteins – SDS

PAGE, Native PAGE, Gradient gel electrophoresis, 2D gel electrophoresis, Isoelectric

focusing, Electrophoresis of Nucleic acids- Agarose Gel Electrophoresis.

Textbooks:

1. Keith W and John W (1995) Practical Biochemistry – Principles & Techniques, 5th

Edn., Cambridge University Press, USA.

2. Avinash U, Kakoll U & Nirmalendu N (1996) Biophysical Chemistry – Principles &

Techniques, , Himalaya Pulbishing House., Delhi, India.

Reference Books

1. Campbell ID and Durek RA (1984) Biological Spectroscopy, 1st

Edn., Benjamin-

Cummings Publishing Company, USA.

2. John Kenkel (1994) Analytical chemistry for technicians, Lewis publishers, Boca.

3. Sharma BK Instrumental methods of chemical analysis, 3rd

Edn., Goel Publishing

Company, India

4. Sadasivam S and Manickam A (1991) Biochemical Methods, 3rd

Edn., New Age

International Publishers, India.

5. Plummer DT (2004) Practical Biochemistry, Tata McGraw Hill Publications, India

6. Channarayappa (2006) Molecular Biotechnology: principles and practices,




What To

whom

When/

Where

(Frequency in

the course)

Max

mark

s

Evidence

collected

Contributin

g to

Course

Outcomes

Dir

ect

Ass

ess

me

nt

Me

tho

ds

CIE

Internal

assessmen

t tests

Student

s

Thrice(Averag

e of the best

two will be

computed)

Blue

books 1,2,3&4

Assignmen

t

Twice(

Average of

the two will

be

computed)

Assignme

nt reports 1,2,3&4

Surprise Once Quiz 1,2,3&4

quiz answers

SE

E Standard

examinatio

n

End of course

(Answering 5

of 10

questions)

100 Answer

scripts 1,2,3&4

Ind

ire

ct

Ass

ess

me

nt End of course

survey

Student

s End of course -

Question-

naire

1,2,3&4,

Assessment

Methods


components


Examination

1 Remember 40 40 30 30


3 Apply 25 15 30 25

4 Analyze 05 05 05 10


6 Create 0 0 0 0

Course Outcome: On completion of this course student will have improved ability to-

1. understand the new emerging technologies in the field

2. Select the appropriate analytical technique for a particular biological, chemical

or environmental sample.

3. Outline the theoretical basis for selected analytical techniques and describe the

instrumentation required.

4. Ability to choose an appropriate analytical approach for specific problem

solving.


BIOCHEMICAL THERMODYNAMICS

Sub Code

Credit

: BT 404

: 3:1:0

CIE

SEE

: 50 Marks

: 50 Marks

Prerequisite: Bioprocess principles & Calculations

Course coordinator: Dr. Chandraprabha M N & Mr. Gokulakrishnan M


1. To provide an introduction to fundamental concepts of the laws of

thermodynamics,

2. To acquire the students with the knowledge for thermodynamic treatment of

pure fluids as well as fluid mixtures and solutions

3. To predict the thermodynamics of phase equilibria

4. To predict the chemical reaction equilibria.

UNIT-1

Introduction: System, surrounding & processes, closed and open systems, intensive &

extensive properties, state and path functions, equilibrium state, reversible and

irreversible processes. First Law of Thermodynamics: General statement of first law of

thermodynamics, first law for cyclic process, Non-flow process, flow process.

UNIT-2

Second law of thermodynamics & P-V-T behaviour: General statement of the second

law, concept of entropy, the Carnot principle, calculation of entropy changes, Clausius

inequality, entropy and irreversibility, third law of thermodynamics. P-V-T behaviour of

pure fluids, equations of state and ideal gas law, processes involving ideal gas law:

constant volume, constant pressure, constant temperature, adiabatic and polytropic

processes. Equations of real gases, principles of corresponding states, compressibility

charts.

UNIT-3

Thermodynamic Properties of Pure Fluids: Derived properties, work function, Gibbs

free energy, relationships among thermodynamic properties. Fundamental property

relations, Maxwell’s relations, Clapeyron equation, entropy-heat capacity relation,

Effect of temperature on U, H & Entropy (S), relationships between Cp & Cv, Gibbs-

Helmholtz equation. Fugacity, fugacity coefficient, Determination of fugacity of pure

gases, fugacities of solids and liquids. Activity and activity coefficient, Thermodynamic

diagrams. Properties of solutions.

UNIT-4

Course

Outcomes

Program Outcome

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

understand the

new emerging

technologies in

the field

X X X X X X X

Select the

appropriate

analytical

technique for a

particular

biological,

chemical or

environmental

sample.

X X

Outline the

theoretical

basis for

selected

analytical

techniques and

describe the

instrumentation

required.

X X X

Ability to

choose an

appropriate

analytical

approach for

specific

problem solving

X X X X X

Properties of Solutions & Phase Equilibria: Partial molar properties, Chemical potential,

Gibbs-Duhem equation & its applications, Henry’s law & Raoult’s law. Criteria of phase

Equilibria, criterion of stability, Duhem's theorem, Vapour- Liquid Equilibria: VLE in ideal

solutions, Consistency test for VLE data, calculation of activity coefficients using Gibbs -

Duhem equation, Liquid-Liquid Equilibrium diagrams.

UNIT-5

Biochemical Energetics: Coupled reactions, Reaction Stoichiometry, criteria of

biochemical reaction equilibrium, equilibrium constant and standard free energy

change, effect of temperature, pressure on equilibrium constants and other- factors

affecting equilibrium conversion.

Textbooks:

1. Smith JM and Van Ness HC (2004) Introduction to Chemical Engineering

thermodynamics, 6th

Edition, McGraw Hill Publications, USA.

2. Narayanan KV (2001) A Textbook of Chemical Engineering Thermodynamics,

Prentice Hall Publication, India.

Reference Books

1. Rao YVC (1992) Chemical Engineering Thermodynamics, New Age International,

India.

2. Segel IH (1993) Biochemical Calculations, 2nd

Edn., John Wiley & Sons, USA.

3. Shuler ML and Kargi F (2001) Bioprocess Engineering, 2nd

Edn., Prentice Hall

International, NJ, USA.

4. Bailey JE and Ollis DF (2010) Biochemical Engg. Fundamentals, 2nd

Edition, McGraw

Hill, New York, USA.

Delivery: Regular black Board teaching and interaction through tutorial class


What To

whom

When/

Where

(Frequency in

the course)

Max

mark

s

Evidence

collected

Contributin

g to

Course

Outcomes

Dir

ect

Ass

ess

me

nt

Me

tho

ds

CIE

Internal

assessmen

t tests Student

s

Thrice(Averag

e of the best

two will be

computed)

30 Blue

books 1,2,3&4

Assignmen Once 10 Assignme 2,3&4

t nt reports

Surprise

Test/

Tutorial

Test

Once 10 Blue

books 3&4

SE

E Standard

examinatio

n

End of course

(Answering 5

of 10

questions)

100 Answer

scripts 1,2,3&4

Ass

ess

me

nt

Me

tho

d

End of course

survey

Student

s End of course -

Question-

naire

CIE and SEE evaluation


Examination

1 Remember 10 10 10 10


3 Apply 60 50 40 40

4 Analyze 0 0 0 0

5 Evaluate 0 20 30 30

6 Create 0 0 0 0


1. Utilize the knowledge of the fundamental concepts of the laws of

thermodynamics and apply the first and second laws of thermodynamics to

identify, formulate and solve engineering problems.

2. To estimate the thermodynamic properties, such as enthalpies, entropies,

Gibbs energies, fugacity coefficients, and activity coefficients of pure fluids as

well as fluid mixtures.

3. To predict equilibrium compositions of mixtures under phase

4. To predict the feasibility and equilibrium constant of chemical reactions.


Course outcome

Program Outcome


Understand the

fundamental

concepts of the

laws of

thermodynamics

and apply the first

and second laws

of

thermodynamics

to identify,

formulate and

solve engineering

problems.

X X X X

Understand the

procedures for

estimating the

thermodynamic

properties, such

as enthalpies,

entropies, Gibbs

energies, fugacity

coefficients, and

activity

coefficients of

pure fluids as well

as fluid mixtures.

X X X X X

Predict

equilibrium

compositions of

mixtures under

phase

X X X X X

To predict the

feasibility and

equilibrium

constant of

chemical

reactions.

X X X

MOLECULAR BIOLOGY

Sub Code

Credit

: BT 405

: 3:1:0

CIE

SEE

: 50 Marks

: 50 Marks

Prerequisite: Cell Biology & genetics

Course coordinator: Dr. Channarayappa and Dr. P. Dhamodhar

Objectives of the course: The course will provide to:

1. Learn the fundaments of molecular biology

2. Understand the principle and mechanism of transcription

3. Understand the principle, mechanism and regulation of translation

4. Study the biological significance of gene expression regulation and genome

modifications

UNIT-1

Introduction to Molecular Biology: Scope of molecular biology. Genomes: genetic

material, gene structure and functions. Concept of central dogma of molecular biology.

DNA replication: Principle, General features of DNA replication; DNA replication in

prokaryotes: prokaryotic DNA polymerases, mechanism of E. coli DNA replication and

other DNA replication models (rolling circle model, unidirectional replication). DNA

replication in eukaryotes: eukaryotic: DNA polymerases, mechanism of DNA replication

and chromosome-end replications, replication fidelity, DNA damage & Repair: causes,

types of mutations, repair mechanisms (direct reversal, excision, and SOS).

UNIT-2

Transcription: What is transcription? Structure and sequences of prokaryotic and

eukaryotic genes. Components of transcription unit. Bacterial and eukaryotic RNA

polymerases, transcription factors, mechanism of transcription in prokaryotes and

eukaryotes, transcriptional apparatus. Posttranscriptional processing of RNA: 5’ and 3’

end modifications, splicing (type-I, type II and spliceosome-mediated), alternative

splicing and its biological significance, exon shuffling and RNA editing.

UNIT-3

Translation: Principle, genetic code and codons. Components of translation. Activation

of tRNA. Mechanism of translation: initiation, elongation and termination of protein

synthesis. Main differences between prokaryotic and eukaryotic translation. Post

translational modifications: cleavage, methylation, sulfur-sulfur bridges, protein splicing,

folding, glycosylation, myristilation. Protein targeting: signal hypothesis and

cotranslational processing, transportation. Inhibitors of transcription and protein

synthesis.

UNIT-4

Gene Expression Regulation: Regulation of gene expression in prokaryotes: Operon

model: significance of operons, lac and trp operons; positive versus negative regulation.

Role of sigma factors in gene expression regulation. Gene rearrangements in gene

expression regulation. Eukaryotic gene expression regulation: Role of upstream

activating sequences and regulatory proteins. Transcriptional and translational control

of gene expression. mRNA turnover kinetics, Gene silencing: anti-sense RNA, RNAi.

Posttranslational regulation, protein degradation and turnover.

UNIT-5

Genome Modifications: Genetic recombination in bacteria and viruses, site-specific

recombination as in meiosis, illegitimate recombination. Recombination repair

mechanisms. Role of recombination and transposition in evolution of new genes.

Transposons and mechanism of transposition, insertion sequences in bacteria, mobile

elements of maize (McClintock’s work). Biological significance of transposition.

Text Books:

1. Alberts B, Johnson A, Lewis J, Raff M, Roberts K, and Walters P (2002) Molecular

biology of the cell, 4th

edn. Garland Science. New York & London.

2. Lodish H, Berk A, Matsudaira P, Kaiser CA, Krieger M, Scott M.P, Zipursky S.L, and

Darnell J (2004) Molecular biology, 5th

edn. W.H. Freeman and Company, New York.


4. Channarayappa (2013) Molecular Biology: Universities Press (India) Pvt Ltd. (in

print)

Reference Books:

1. Friedberg EC, Walker GC and Siede W (1995) DNA repair and Mutagenesis.

Washington, DC: ASM Press.

2. Karp G (2005) Cell and Molecular Biology: concepts and experiments, 4th

edn., John

Wiley & Sons, Inc.

3. Cooper GM and Hausman RE (2006) The Cell: A Molecular Approach, 4th

edn. ASM

Press and Sinauer Associates

4. Freifelder D (2007) Molecular Biology. Narosa Publishing House

5. Channarayappa (2006) Molecular Biotechnology: Principles and Practices,




What To

whom

When/

Where

(Frequency

in the

course)

Max

mark

s

Evidence

collected

Contributi

ng to

Course

Outcomes

Dir

ect

Ass

ess

me

nt

Me

tho

ds

CIE

Internal

assessmen

t tests

Student

s

Thrice(Avera

ge of the

best two will

be

computed)


s-room

open book

assignmen

t

Once 10 Assignment

reports 1,2,3&4

Surprise

Test Once 10 Blue books 1,2,3&4

SE

E Standard

examinati

on

End of

course

(Answering 5

of 10

questions)

100 Answer

scripts 1,2,3&4

Ind

ire

ct

Ass

ess

me

nt End of course

survey

Student

s

End of

course -

Questionnai

re

1,2,3&4,

Assessmen

t methods


components

SL

NO.

Bloom’s

Category

Test 1 Test 2 Test 3 Tutorial CIE/SEE

1 Remember 25 25 20 30 20

2 Understand 25 25 30 20 20

3 Apply 30 30 20 10 35

4 Analyze 15 15 20 25 20

5 Evaluate 5 5 10 10 5

6 Create 0 0 0 5 0


1. Emphasize on the basic aspects of molecular biology; the key areas, the

conventions followed, and the scope of molecular biology.

2. Acquire working knowledge on the mechanism of transcription and translation

stepwise and their applications in the research.

3. Execute different techniques to genetically engineer the gene expression

regulation that is very essential for controlled protein production in the

industry.

4. Evaluate different causes and mechanisms responsible for genetic

modifications and their consequences on human health and environment.


Course Outcomes

Program Outcome


Understand the basic

aspects of molecular

biology; the key areas,

the conventions

followed, and the scope

of molecular biology.

X X X X X

Acquire working

knowledge on the

mechanism of

transcription and

translation stepwise and

their applications in the

research.

X X X X X

Execute different

techniques to

genetically engineer the

gene expression

regulation that is very

essential for controlled

protein production in

X X X X

the industry.

Evaluate different

causes and mechanisms

responsible for genetic

modifications and their

consequences on

human health and

environment.

X X X X X X

HUMAN PHYSIOLOGY

Sub code

Credit

: BT 406

: 3:0:0

CIE

SEE

: 50 Marks

: 50 Marks

Prerequisite: Cell biology and genetics

Course coordinator: Dr. Prabha M and Mr. Lokesh K. N.

Objectives of the course: The course will provide to:

1. The anatomy and physiology of tissue organization within the human body and

their relation with the importance of homeostasis to health.

2. The structure and function of muscle tissue and muscle cell contraction related

to function of the heart.

3. The study on major endocrine glands and tissues of the body, reproductive

system and their functions.

4. The maintenance of various organs and knowledge of tissues such as nervous

system coordination, digestion, respiration and excretion.

UNIT-1

Tissue Organization, Haemopoitic and Circulatory System: Basic types, location, types

and function, epithelial, connective tissues (Blood, Bones, cartilages), muscular tissues,

Specialized cells (Nerve cells), properties, mechanism of muscle contraction and

relaxation,. Lymph and Lymphatic system, anatomical features of heart and their

accessory blood vessels, Heart Beat. Blood Pressure, ECG.

UNIT-2

Digestive and excretory system: Overview of digestive system, functional anatomy of

digestive systems and their accessory organs, Physiology of Digestion and Absorption of

carbohydrates, lipids and proteins. Kidneys; Anatomy and physiology, Nephron -its

structure & Functions. Mechanism and regulation of urine formation.

UNIT-3

Respiratory system& Skeletal System: Introduction; structure of respiratory organs;

Mechanism of breathing; pulmonary air volumes, Gas exchange in the lungs. Kinds of

respiration; Transport of respiratory gases in the blood, skeletal system.

UNIT-4

Endocrine system: Introduction; Pituitary gland; Thyroid gland; Parathyroid gland;

Pancreas; Adrenal glands and its hormone regulation; Sex glands; Gastrointestinal

mucosa; Thymus gland; Pineal gland; Summary of different endocrine glands their

hormones and functions.

UNIT-5

Nervous System: Introduction; Role of nervous system; Types of neurons. Types of glial

cells and its function. Main properties of nervous tissue Mode of action of nerves;

Conduction of nerve impulses; Central nervous system; The brain; The spinal cord;

Peripheral nervous system.

Textbooks:

1. Waugh A & Grant A (2000) Ross & Wilson’s Anatomy and Physiology in Health and

Illness, 9th

Edn., Churchill Livingstone Publications, USA.

2. Sembulingam K & Prema S (2002) Essentials of Medical Physiology, 3rd

Ed., Jaypee

Publications, Delhi, India.

Reference Books

1. Sujit K Chaudhari, (2003) Concise Medical Physiology, 5th

Edn., New Central Book

Agency Pvt. Ltd., Delhi, India

2. Sembulingam and Prema SK (2002) Essentials of Medical Physiology, 3rd

Edn.,

Jaypee Publications, India.

3. Daniel DC (2006) Human Biology, 5th

Edn., Jones and Barlette Publishers Pvt. Ltd.,

USA.




What To

whom

When/

Where

(Frequency in

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

the course)

Dir

ect

Ass

ess

me

nt

Me

tho

ds

CIE

Internal

assessme

nt tests

Student

s

Thrice(Averag

e of the best

two will be

computed)

30 Blue

books 1,2,3&4

Class-

room

open

book

assignme

nt

Twice(

Average of

the two will

be computed)

10 Assignmen

t reports 1,2,3&4

Case

analysis Once 6

Case

solutions 1,2,3&4

Surprise

quiz Once 4

Quiz

answers 1,2,3&4

SE

E Standard

examinati

on

End of course

(Answering 5

of 10

questions)

100 Answer

scripts 1,2,3&4

Ind

ire

ct

Ass

ess

me

nt

Me

tho

ds

End of course

survey

Student

s End of course -

Question-

naire

1,2,3&4

assessment

methods


components


1 Remember 20

2 Understand 20

3 Apply 35

4 Analyze 20

5 Evaluate 5

6 Create 0


1. To focus on the anatomy and physiology relationship in the human body

function and vital processes of the various tissue structures in the

understanding of human body parts, systems and their homeostatic functions.

2. To identify fundamental movements of human body relating to muscles and

joints. The cardiovascular system transports oxygen and nutrients to the cells

and transports wastes away from the cells.

3. To understand the basic physiological effects of endocrine glands, which

enables the cells to function, grow, reproduce which are essential for the

survival of the species.

4. To know how the nervous system coordinates, integrates the functions of body

systems so that the regulation of function is maintained normally in digestion,

respiration and excretion that regulates metabolic processes to maintain a

relatively constant internal environment, yet meet the changing needs of the

body.


Course Outcomes

Program Outcome


To focus on the anatomy and physiology

relationship in the human body function and

vital processes of the various tissue

structures in the understanding of human

body parts, systems and their homeostatic

functions

X X X

To identify fundamental movements of

human body relating to muscles and joints.

The cardiovascular system transports oxygen

and nutrients to the cells and transports

wastes away from the cells.

X X X

To understand the basic physiological effects

of endocrine glands, which enables the cells

to function, grow, reproduce which are

essential for the survival of the species.

X X

To know how the nervous system

coordinates, integrates the functions of body

systems so that the regulation of function is

maintained normally in digestion, respiration

and excretion that regulates metabolic

processes to maintain a relatively constant

X X X X X X

HEAT & MASS TRANSFER LAB

Sub Code

Credit

: BT407L

: 0:0:1

CIE

SEE

: 50 Marks

: 50 Marks

Prerequisite: Heat & Mass transfer

Course coordinator: Mr. Samrat K & Dr.Chandraprabha M N

Objectives of the lab: The course will provide to

1. To analyze and interpret the results of their experimental work.

2. To study the individual and overall heat transfer coefficients of condenser and

heat exchangers.

3. To analyze the distillation, absorption and extraction operations.

LABORATORY

LIST OF EXPERIMENTS

1. Critical radius

2. Vertical condenser.

3. Horizontal condenser.

4. Heat transfer in packed bed.

5. Heat transfer in jacketed vessel

6. Heat transfer in Shell & Tube heat exchanger

7. Diffusion of organic vapors in air.

8. Simple Distillation.

9. Steam Distillation.

10. Drying Characteristics.

11. Adsorption studies.

12. Packed Column Distillation

13. Single & Multi stage extraction

14. Single & Multi stage leaching


Reference Books

1. Alan S Foust, Wenzel LA, Clump CW, Maus L and Anderson LB (2008) Principles of

Unit Operations, 2nd Edn., John Wiley & Sons, USA.

2. Coulson and Richardson’s (2006) Chemical Engineering, Vols I & II, 5th

Edn., Reed

Educational and Professional Publishing Ltd., USA.


sessions


What To

whom

When/

Where

(Frequency

in the

course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

ess

me

nt

Me

tho

ds

CIE

Internal

assessmen

t tests

Student

s

Once 30 Blue

books 1,2,3&4

Lab

Assesmen

t

Continuous 10 Attendanc

e 1,2,3&4


SE

E Standard

examinati

on

End of

course 50

Answer

scripts 1,2,3&4

Ass

e

ssm

e

nt End of course

survey

Student

s

End of

course -

Question-

naire ---


components

SL NO. Bloom’s Category Lab exam Semester-End Exam

1 Remember 20 20

2 Understand 20 20

3 Apply 35 35

4 Analyze 20 20

5 Evaluate 5 5

6 Create 0 0


1. Predict the individual and overall heat transfer coefficients of condenser and

heat exchangers.

2. Estimate the heat transfer rate in jacketed vessel and packed bed.

3. Determine mass transfer coefficient of mass transfer operations.

4. Estimate the efficiency of extraction, distillation units


Course

Outcome

s

Program Outcome

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO1

0

PO1

1

PO1

2

Predict

the

individual

and

overall

heat

transfer

coefficien

ts of

condense

r and

heat

exchange

rs.

X X X X

Estimate

the heat

transfer

rate in

jacketed

vessel

and

X X X X X X X

packed

bed.

Determin

e mass

transfer

coefficien

t of mass

transfer

operation

s.

X X X X X

Estimate

the

efficiency

of

extraction

,

distillatio

n units

X X

BIOANALYTICAL TECHNIQUES LAB

Sub Code

Credit

: BT408L

: 0:0:1

CIE

SEE

: 50 Marks

: 50 Marks

Prerequisite: Bioanalytical Techniques

Course coordinator: Dr. Ahalya N & Dr Sravanti V

Objectives of the course:

1. To Introduce students to the basic experimental techniques

2. To develop students’ abilities to obtain accurate and concordant quantitative

analytical results.

3. To introduce students to the procedures required to efficiently and effectively

utilize the quantitative instrumental analytical instrumentation commonly used

in industrial and research laboratories.

4. The tools and techniques by which biological molecules are isolated, separated,

identified, and analyzed

LABORATORY

LIST OF EXPERIMENTS

1. Calibration of pH meter and preparation of Buffers and standard solutions

2. Thin layer Chromatographic separation of sugars / amino acids

3. Column Chromatography

4. Two dimensional paper chromatography of amino acids

5. Electrophoresis separation of Proteins

6. Elution from chromatography column and estimation

7. DNA separation by Agarose Gel Electrophoresis

8. Native PAGE

9. Spectrophotometric estimation of biomolecules

10. Separation and estimation of chlorophyll

11. Fractionation of cellular components

12. Isolation of chloroplasts.

13. Separation of amino acids/ Nucleic acids by using HPLC

14. Separation of lipids using GLC


Reference Books

1. Sadasivam S and Manickam A (1991) Biochemical Methods, 3rd

Edn., New Age

International Publishers, India.

2. Plummer DT (2004) Practical Biochemistry, Tata McGraw Hill Publications, India

3. Channarayappa (2006) Molecular Biotechnology: principles and practices,



sessions


What To

whom

When/

Where

(Frequency

in the

course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

ess

me

nt

Me

tho

ds

CIE

Internal

assessme

nt tests

Student

s

Once 30 Blue

books 1,2,3&4

Lab

Assesme

nt


e 1,2,3&4


SE

E Standard

examinati

on

End of

course 50

Answer

scripts 1,2,3&4

Ind

ire

ct

Ass

ess

me

nt

Me

tho

ds

End of course

survey

Student

s

End of

course -

Question-

naire

1,2,3&4 and

assessment

methods


components

ASSESSMENT PATTERN:

SL NO. Bloom’s Category Lab Internal test Semester-End Exam

1 Remember 10 10

2 Understand 10 10

3 Apply 20 10

4 Analyze 20 30

5 Evaluate 20 20

6 Create 20 20

Marks distribution:

CIE: 50 Laboratory internal tests will be conducted 30 marks. Lab

Assessment/Record will be conducted 20 marks.

Course Outcome: On completion of this course student will have improved ability to

1. Identify the most useful technique for a given bioanalytical problem,

2. Interpret and use the results from a given bioanalytical technique,

3. Understand the physical, chemical and instrumental fundamentals underlying

these measurements

4. Critically assess advances within the field of bioanalytical chemistry.


Course

outcome

Program Outcome

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

P

O

10

P

O

11

P

O

12

Identify the

most useful

technique for

a given

bioanalytical

problem

X X X X X

Interpret and

use the

results from

a given

bioanalytical

technique

X X

Understand

the physical,

chemical and

instrumental

fundamental

s underlying

these

measuremen

ts

X X

Critically

assess

advances

X X X

within the

field of

bioanalytical

chemistry.

MOLECULAR BIOLOGY LAB

Sub Code

Credit

: BT409L

: 0:0:1

CIE

SEE

: 50 Marks

: 50 Marks

Prerequisite: cell biology and genetics

Course coordinator: Dr. Sharath, R. and Dr. Channarayappa

Course Objective

1. Describe the general structures of both prokaryotic and eukaryotic organisms.

2. Involve the use of recombinant DNA technology and other Common gene

analysis techniques.

3. Will develop basic knowledge and skills in cell and molecular biology and

become aware of the complexity and harmony of the cell.

LAB SESSIONS

LIST OF EXPERIMENTS

1. Study of cell structure (Prokaryotes & Eukaryotes).

2. Study of mutants of Drosophila and maintenance /special chromosome

3. Study of Mitotic stages by Squashing Technique (Onion root tips).

4. Study of Meiotic stages in Allium cepa

5. Isolation of genomic DNA (plant / animal/ microbial sources – any one).

6. Quantification of nucleic acids by spectrophotometric, Gel and by densitometry

methods.

7. Polymerase Chain Reaction (Programming and working).

8. Agarose gel electrophoresis of DNA.

9. Restriction mapping (Single or double digestion).

10. Isolation of plasmid DNA

11. Replica plating method

12. DNA Ligation

13. Bacterial conjugation

14. Extraction of DNA figments from Agarose gel (column or solution based

method)

Note: Any 12experiments must be performed

Reference Books

1. Channarayappa (2013) Molecular Biology: Universities Press (India) Pvt Ltd.

2. Alberts B, Johnson A, Lewis J, Raff M, Roberts K, and Walters P (2002)

Molecular biology of the cell, 4th

edn. Garland Science. New York & London

3. Karp G (2005) Cell and Molecular Biology: concepts and experiments, 4th

edn.,

John Wiley & Sons, Inc.

4. Cooper GM and Hausman RE (2006) The Cell: A Molecular Approach, 4th

edn.

ASM Press and Sinauer Associates


sessions


What To

whom

When/

Where

(Frequency

in the

course)

Max

marks

Evidence

collected

Contributing

to Course

Outcomes

Dir

ect

Ass

ess

me

nt

Me

tho

ds

CIE

Internal

assessme

nt tests

Student

s

Once 30 Blue

books 1,2,3&4

Lab

Assesmen

t


e 1,2,3&4


SE

E Standard

examinati

on

End of

course 50

Answer

scripts 1,2,3&4

Ind

ire

ct

Ass

ess

me

nt

Me

tho

ds

End of course

survey

Student

s

End of

course -

Question-

naire

1,2,3&4 and

assessment

methods


components


1 Remember 10

2 Understand 20

3 Apply 10

4 Analyze 30

5 Evaluate 30

6 Create

Course Outcome

1. Understand, and perform, the most important methods in molecular biology.

2. It prepares students for careers in fields that require advanced knowledge of

molecular biology, in particular those that relate to human health and welfare.

3. Understand the significance of molecular biology of human health, disease and

treatment.

4. Will gain insight into the most significant molecular and cell-based methods

used today to expand their understanding of biology.


Course

Outcomes

Program Outcome

PO

1

PO

2

PO3 PO

4

PO

5

PO6 PO

7

PO8 PO

9

PO

10

PO

11

Understand,

and

perform, the

most

important

methods in

molecular

biology.

X X 1

It prepares

students for

careers in

fields that

require

advanced

knowledge

of molecular

biology, in

particular

those that

relate to

human

health and

welfare.

X X X

Understand

the

significance

of molecular

biology of

human

health,

disease and

treatment.

X X X

Will gain

insight into

the most

significant

molecular

and cell-

based

methods

used today

to expand

their

understandi

ng of biology

X X

Syllabus 3 and 4 Semester

Documents

Transcript of Syllabus 3 and 4 Semester