Supplementary Figure 3

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Supplementary Figure Supplementary Figure 3 Involvement of the oxidative stress in the response of iPrEC cells to Doxo and DTX treatments. Flow cytometric evaluation of (a) ROS generation and (b) apoptosis, carried out in the drug-exposed iPrEC cells, previously loaded with NAC agent. (c) Analysis of ROS production performed in Doxo- and DTX-treated iPrEC transfectants. % ROS a iPrEC - + - + - + NAC Doxo (0,75 µM) DTX (10 nM) Untreated % Apoptosis (Ann V+/PI- cells) - + - + - + NAC Doxo (0,75 µM) DTX (10 nM) Untreated b iPrEC c USP2a WT USP2a MUT EV ROS generation Untreated Doxo (0,75 µM) Untreated DTX (10 nM)

description

Supplementary Figure 3. a. iPrEC. Untreated. Untreated. Doxo (0,75 µM). DTX (10 nM). % ROS. % Apoptosis (Ann V+/PI- cells). b. iPrEC. -. -. -. -. -. -. +. +. +. +. +. +. c. NAC. NAC. EV. USP2a MUT. USP2a WT. Untreated. Untreated. Doxo (0,75 µM). Doxo - PowerPoint PPT Presentation

Transcript of Supplementary Figure 3

Page 1: Supplementary Figure 3

Supplementary Figure 3

Supplementary Figure 3 Involvement of the oxidative stress in the response of iPrEC cells to Doxo and DTX treatments.

Flow cytometric evaluation of (a) ROS generation and (b) apoptosis, carried out in the drug-exposed iPrEC cells, previously loaded with NAC agent. (c) Analysis of ROS production performed in Doxo- and DTX-treated iPrEC transfectants.

% R

OS

aiPrEC

- + - + - + NAC

Doxo(0,75 µM)

DTX(10 nM)

Untreated

% A

popt

osis

(A

nn V

+/P

I- c

ells

)

- + - + - + NAC

Doxo(0,75 µM)

DTX(10 nM)

Untreated

biPrEC

c USP2aWTUSP2aMUTEV

ROS generation

Untreated Doxo (0,75 µM)

Untreated DTX (10 nM)

Supplementary figure S1.

Supplementary figure S1.

Supplementary Figure 2A

Supplementary Figure 2A

Supplementary figure 2.

Supplementary figure 2.

Supplementary Figure 1. Supplementary Figure 2 Supplementary Figure 3 A B.

Supplementary Figure 1. Supplementary Figure 2 Supplementary Figure 3 A B.

Supplementary Information SUPPLEMENTARY … · SUPPLEMENTARY INFORMATION 2 | RESEARCH Supplementary Information 3 Fig. S2 Figure S2: Carbon Rearrangement Networks Used in Various

Supplementary Information SUPPLEMENTARY … · SUPPLEMENTARY INFORMATION 2 | RESEARCH Supplementary Information 3 Fig. S2 Figure S2: Carbon Rearrangement Networks Used in Various

Supplementary figure 1A

Supplementary figure 1A

Supplementary Figure 1A HNF4AWNT5AVANGL1PCLB1 Supplementary Figure 1B Supplementary Figure 1. (A) HNF4A TFBSs of the three genes: VANGL1, WNT5A and PLCB1.

Supplementary Figure 1A HNF4AWNT5AVANGL1PCLB1 Supplementary Figure 1B Supplementary Figure 1. (A) HNF4A TFBSs of the three genes: VANGL1, WNT5A and PLCB1.

Supplementary Figure 1

Supplementary Figure 1

1 Supplementary Figures 2 3 Supplementary Figure 1: TRPV1 expression … · 2017-05-10 · 1 1 Supplementary Figures 2 3 Supplementary Figure 1: TRPV1 expression in the ACC of both

1 Supplementary Figures 2 3 Supplementary Figure 1: TRPV1 expression … · 2017-05-10 · 1 1 Supplementary Figures 2 3 Supplementary Figure 1: TRPV1 expression in the ACC of both

Supplementary Figure S1

Supplementary Figure S1

Supplementary Figures: Supplementary Figure 1 – PANCAN ...df6sxcketz7bb.cloudfront.net/manuscripts/88000/88755/jci.insight.88755.sd.pdfSupplementary Figures: Supplementary Figure

Supplementary Figures: Supplementary Figure 1 – PANCAN ...df6sxcketz7bb.cloudfront.net/manuscripts/88000/88755/jci.insight.88755.sd.pdfSupplementary Figures: Supplementary Figure

media.nature.com · 1 Supplementary Materials Supplementary Table S1 Supplementary Table S2 Supplementary Table S3 Supplementary Figure S1 Supplementary Figure S2 Supplementary Figure

media.nature.com · 1 Supplementary Materials Supplementary Table S1 Supplementary Table S2 Supplementary Table S3 Supplementary Figure S1 Supplementary Figure S2 Supplementary Figure

Supplementary FIGURE 3

Supplementary FIGURE 3

Supplementary Figure 1 Reported (nonredundant) protein … · 2017-07-26 · Supplementary Figure 3 Reported (nonredundant) protein interactions in PSD scaffold interactors complexes

Supplementary Figure 1 Reported (nonredundant) protein … · 2017-07-26 · Supplementary Figure 3 Reported (nonredundant) protein interactions in PSD scaffold interactors complexes

LEGENDS TO SUPPLEMENTARY FIGURES Supplementary Figure 1 ...

LEGENDS TO SUPPLEMENTARY FIGURES Supplementary Figure 1 ...

Supplementary figure 5

Supplementary figure 5

Supplementary Materials Supplementary Figure Legends ...

Supplementary Materials Supplementary Figure Legends ...

Supplementary Figure 4

Supplementary Figure 4

Supplementary Figure 2

Supplementary Figure 2

Supplementary Figure 1 - Nature · Supplementary Figure 3 ... NM_001077203 SENP7 1.921 0.980 ... Microsoft Word - Nguyen_Tavazoie_nc_supp_020616.docx

Supplementary Figure 1 - Nature · Supplementary Figure 3 ... NM_001077203 SENP7 1.921 0.980 ... Microsoft Word - Nguyen_Tavazoie_nc_supp_020616.docx