Standard Microbiological Practices

UT Biosafety Officehttp://biosafety.utk.edu

Standard Microbiological Practices (SMP)

What are they?• Basic “hygiene” practices that apply to all labs

and clinics that manipulate microorganisms or biological materials that contain microorganisms.

What’s their purpose?• To minimize the spread of contamination

generated through the lab processes to protect both personnel and the environment.

SMP’s apply to a broad spectrum of activities including:• Manipulation of any microbes including bacteria, viruses, fungi,

and protozoa.• Manipulation of materials that may contain microbes including

animal and plant tissues, soil samples, and water samples.• Receiving, processing, and testing of diagnostic samples.• Research involving recombinant DNA molecules, transgenic

animals, or genetically modified plants.• Manipulation of animals or plants that are experimentally

infected with microbes.• Work with biological toxins and other bioactive molecules.

Standard Microbiological Practices (SMP)

BiohazardsThis symbol indicates that there is a biological material that can cause harm to yourself, others in the lab, or the environment. Use caution whenever you see this symbol. Speak to your advisor for additional information about the biohazards present in the lab.

Lab Hygiene: The Basics• Do not store food or drink in lab.• Do not consume food or drink in the lab.• Do not apply facial cosmetics (including

lipstick, lip balm, etc.) in lab.• Keep personal items

out of areas wherebiological or otherhazardous materialsare manipulated orstored.

Lab Hygiene: Hand Washing• Extremely important SMP!

– Reduces accidental infection risk

– Minimizes spread of contamination

• Use soap and running water.

• Lather for 20-30 seconds.• Waterless hand cleaners

may be used if soap/water aren’t available, but hands should be properly washed as soon as possible.

Proper response to an exposurecan reduce your risk of an infection…

If potentially infectious materials enter your body through:– A cut or puncture with a contaminated sharp– Entry through broken skin– A splash to the eyes, nose or mouth– Accidental ingestion

You must:– Flush the exposed area (15 minutes)– Immediately report to your supervisor

Personal Protective Equipment (PPE)

Use personal protective equipment (PPE) when handling or manipulating biological materials (including wastes) to prevent accidental exposures.

Some types of PPE are gloves, lab coat, safety goggles, and safety glasses.

Sharps Safety Some examples of sharps are needles, scalpels, and contaminated broken glassware. These materials must be handled in a safe manner. • Do not handle sharps with 2 hands.• Don’t pass a sharp device from one hand to

another.• Don’t recap needles or scalpels!

Waste Segregation

There are different types of biological waste. Sometimes biological waste is mixed with chemical waste. Speak with your advisor on how to dispose of your research waste properly. Research waste does not go into a regular trash can.

Disinfect all contaminated surfaces after procedures.

• Clean surfaces of visible debris before you disinfect. • Know your disinfectant before you use it. • Refer to the manufacturer’s instructions. • Questions to ask before selecting a disinfectant:

– Is the product effective for organisms of concern?– Is the product designed for your

application?– What are the hazards to the user

and what PPE is required?– What is the dilution ratio?– Contact time?– Shelf-life?

Transporting Biological MaterialsBiological materials should be stored in primary containers that are designed to contain the material inside. Use sample containers that are manufactured for that purpose.Do not hand-carry primary containers outside the lab without a secondary container. A secondary container is intended to capture leakage or spillage from the primary container. Therefore a suitable secondary container for moving materials within a building should be: • Have a lid if used for transporting primary containers that are open.• Leak-proof on the sides and bottom• Non-breakable• Have raised sides• Easy to grasp (ideally, have a handle)

Containment devices

Below is a chemical fume hood. This is only to used for handling chemicals.

Above is a biosafety cabinet. This is to be used for handling biological materials that are infectious.

Get training from your supervisor PRIOR to using either containment device.

If you have any questions, contact the Biosafety

OfficeBrian Ranger, MS, SM(NRCM), CBSP

Biosafety Officer, UTK/UTIA/GSM974-1938 or branger@utk.edu

ORSarah DiFurio, MS, RBPBiosafety Specialist, UTK/UTIA/GSM974-9836 or sbottomL@utk.edu

http://biosafety.utk.edu

Quiz TimeTo complete the Staying Safe in the Laboratory Training Module, please click here for the quiz.