Specimen taken from feverish patient what is diagnosis.

95
Specimen taken from feverish patient what is diagnosis

Transcript of Specimen taken from feverish patient what is diagnosis.

Page 1: Specimen taken from feverish patient what is diagnosis.

Specimen taken from feverish patient what is diagnosis

Page 2: Specimen taken from feverish patient what is diagnosis.

Banana shape P.f gametocytes Vector: female Anopheles mosquito Mode of infection: injection of sporozoite during

Anopheles bite.

1.Falcipram gametocyte

Page 3: Specimen taken from feverish patient what is diagnosis.
Page 4: Specimen taken from feverish patient what is diagnosis.

Plasmodium ring stage

Page 5: Specimen taken from feverish patient what is diagnosis.

2.Plasmodium ring stage Diagnosis: blood film (thin and thick) Vector: female Anopheles Infective stage: Sporozoite

Page 6: Specimen taken from feverish patient what is diagnosis.
Page 7: Specimen taken from feverish patient what is diagnosis.

3.Trichomonas vaginalis Diagnosis: vaginal, urethral smear,

occasionally in urine analysis Trophozoite is the infective stage. transmitted sexually.

Page 8: Specimen taken from feverish patient what is diagnosis.
Page 9: Specimen taken from feverish patient what is diagnosis.

4 .Eggs of Taenia species

-T.saginata -T. solium -Echinoccus granulosus

Page 10: Specimen taken from feverish patient what is diagnosis.
Page 11: Specimen taken from feverish patient what is diagnosis.
Page 12: Specimen taken from feverish patient what is diagnosis.

5.Taenia saginata (adult tape)

Diagnosis: stool examination for - eggs - gravid segments. Infective stage : cysticercus bovis-in under cooked

meat (beef). Adult habitat small intestine

Page 13: Specimen taken from feverish patient what is diagnosis.
Page 14: Specimen taken from feverish patient what is diagnosis.

6.Soft tick

1. Relapsing fever (endemic).

2. Tick paralysis

Page 15: Specimen taken from feverish patient what is diagnosis.
Page 16: Specimen taken from feverish patient what is diagnosis.

7.Hard tick Rocky mountain spotted

fever Q fever Babesiosis

Page 17: Specimen taken from feverish patient what is diagnosis.

Immature egg

Page 18: Specimen taken from feverish patient what is diagnosis.

8.Adult Fasciola hepatica Diagnosis :egg in

-stools examination -duodenal aspirate. Infective stage : encysted metacercaria (on water plants). Habitat : biliary passage in liver.

Page 19: Specimen taken from feverish patient what is diagnosis.
Page 20: Specimen taken from feverish patient what is diagnosis.

9-FemaleTrichuris trichiura (whip worm)

Diagnosis : egg in stools Infective stage, mode of

infection: ingestion of embryonated egg in soil.

Page 21: Specimen taken from feverish patient what is diagnosis.

Scotch tape preparationClear adhensive tape slide

Page 22: Specimen taken from feverish patient what is diagnosis.

Used in detection of Enterobius vermicularis infection

Collect eggs from perianal area

Scotch tape preparationClear adhensive tape slide

Page 23: Specimen taken from feverish patient what is diagnosis.
Page 24: Specimen taken from feverish patient what is diagnosis.

10.Enterobius vermicularis egg (Pin worm,Oxyuris worm)Diagnosis: Scotch tape preparation to collect eggs from perianal area and microscopical examination.

Page 25: Specimen taken from feverish patient what is diagnosis.
Page 26: Specimen taken from feverish patient what is diagnosis.

11.Schistosoma haematobium egg (Urinary schistosomiasis)Diagnosis: - Urine analysis - Serology in chronic cases. Infective stage :cercaria in

water penetrate skin.

Pathology : periportal fibrosis. ppf

Page 27: Specimen taken from feverish patient what is diagnosis.
Page 28: Specimen taken from feverish patient what is diagnosis.

12 .Schistosoma mansoni egg

in stools specimen (intestinal schistosomiasis) Other methods of diagnosis :

- rectal swab . - rectal snip biopsy. - serology: to detect antibodies

in patient serum.

Page 29: Specimen taken from feverish patient what is diagnosis.
Page 30: Specimen taken from feverish patient what is diagnosis.

13.Leishmania donovani bodies in smear

(skin,B.M,spleen) (amastigotes)

Vector : sand fly . Infective stage: promastigote. for diagnosis of leishmaniasisCulture smear amastigotes from(skin ,BM, spleen) on NNN media

grow and multiply in promastigote form.

Page 31: Specimen taken from feverish patient what is diagnosis.
Page 32: Specimen taken from feverish patient what is diagnosis.

14 .FleasMedical importance: - Plague (Yerisinia pestis). - Murine (endemic )Typhus. -Tunga penetrance (chigger`s disease) Jigger`s

Page 33: Specimen taken from feverish patient what is diagnosis.
Page 34: Specimen taken from feverish patient what is diagnosis.

15.Echinococcus granulosus adultDog cestodeDog take infection after eating hydatid cyst (from infected animals).

Man get hydatid disease by ingestion of egg from infected dog.

Page 35: Specimen taken from feverish patient what is diagnosis.
Page 36: Specimen taken from feverish patient what is diagnosis.

16.Pediculus humanus (head and body lice)

Medical importance: - Epidemic typhus - Trench fever - Epidemic relapsing fever - Pediculosis

Page 37: Specimen taken from feverish patient what is diagnosis.

Diagnosis : - nits visible by naked eye, and fluoresce under UV light (wood's lamp for screening).

Page 38: Specimen taken from feverish patient what is diagnosis.
Page 39: Specimen taken from feverish patient what is diagnosis.

•Smaller than pediculus; about 2mm.

Infest:

•pubic hair mainly

•eye lashes.

• Transmitted by direct contact with infected person or clothes ,it is irritant.

•No disease transmission

8-Phthirus pubis

Page 40: Specimen taken from feverish patient what is diagnosis.
Page 41: Specimen taken from feverish patient what is diagnosis.

17 .Trichuris trichiura egg

Diagnosis : egg in stools .

Infective stage : embryonated egg.

Page 42: Specimen taken from feverish patient what is diagnosis.
Page 43: Specimen taken from feverish patient what is diagnosis.

18 .Musca demostica

Egg—larvae–- pupa--- adult. House fly developmental stage (Life cycle)Medical importance mechanical transmation of virus, bacterial and parasitic diseases.

Page 44: Specimen taken from feverish patient what is diagnosis.
Page 45: Specimen taken from feverish patient what is diagnosis.

19 .Hydatid cyst in liver

* diagnosis can confirmed by: 1 - examination of hydatid fluid

after surgical removal. 2 - serology.

* Infective stage: ingestion of Echinococcus egg (dog cestod).

Page 46: Specimen taken from feverish patient what is diagnosis.
Page 47: Specimen taken from feverish patient what is diagnosis.

20-Taenia (tape worm) T.saginata --cysticercus bovis in beef. T.solium--cysticercus

cellulose in pig.Diagnosis ----eggs or gravid

segment in stools.

Page 48: Specimen taken from feverish patient what is diagnosis.
Page 49: Specimen taken from feverish patient what is diagnosis.

21 .Ascaris lumbricoid adult

Diagnosis : eggs in stools (sometime adult) Infection : ingestion of embryonated egg. (soil transmitted disease) Habitat : small intestine.

Page 50: Specimen taken from feverish patient what is diagnosis.

22. Ascaris lumbricoid

Diagnosis :eggs in stools Infective stage: embryonated

egg.

Page 51: Specimen taken from feverish patient what is diagnosis.

23 . N N N media

used in culture of Leishmania bodies (amastigote)

to diagnose leishmaniasis (visceral & cutaneous).

Amastigotes in smear or aspirate

culture promastigotes.

Page 52: Specimen taken from feverish patient what is diagnosis.
Page 53: Specimen taken from feverish patient what is diagnosis.

24 .Fasciola hepatica eggdiagnosis: finding egg by:

-stools examination -duodenal aspirate infective stage: encysted metacercaria Adult habitat: biliary passage (liver)

Page 54: Specimen taken from feverish patient what is diagnosis.
Page 55: Specimen taken from feverish patient what is diagnosis.

25 . Mosquitoe life cycleEgg—larvae–- pupa--- adult.

Medical importance. Anopheles:

vector of malaria and filaria. Culex : vector of filaria and viruses. Aedes :(viruses) yellow fever, dengue

and rift valley fever.

Page 56: Specimen taken from feverish patient what is diagnosis.
Page 57: Specimen taken from feverish patient what is diagnosis.

26. Cyclops

Vector for transmission of Madina worm

Dracunculus medinesis

Page 58: Specimen taken from feverish patient what is diagnosis.
Page 59: Specimen taken from feverish patient what is diagnosis.

Diarrheal Stool smear what is organisms , how it can be diagnosed ?

Page 60: Specimen taken from feverish patient what is diagnosis.
Page 61: Specimen taken from feverish patient what is diagnosis.
Page 62: Specimen taken from feverish patient what is diagnosis.
Page 63: Specimen taken from feverish patient what is diagnosis.

27-

Page 64: Specimen taken from feverish patient what is diagnosis.

Diagnosis -Stool examination daily for three days for cyst or trophozoites .

-duodenal aspirate examination, or by string (enterotest)

Page 65: Specimen taken from feverish patient what is diagnosis.
Page 66: Specimen taken from feverish patient what is diagnosis.

Feature of human intestinal nematodes

Adult live in intestinal tract. Female are oviparous, i.e. lay

eggs. Humans are host of major

intestinal nematodes of medical importance. Most species are spread by

fecal pollution of soil. soil transmitted disease

Page 67: Specimen taken from feverish patient what is diagnosis.

larvae (free or in egg) develops to its infective stage in soil.

Infection by - swallowing of infective

eggs . (A.lumbricoides ,T.trichiura,

E.vermicularis ) - or penetration of skin by

infective larvae (Hook worms , S.stercoralis) Larvae of A.lumbricoid , S.stercoralis and Hookworms (undergo heart to lung migration).

Page 68: Specimen taken from feverish patient what is diagnosis.

filariform larvae is infective stage of S.stercoralis and Hookworm by penetration of skin.

Rabditiform larvae is used to describe larvae that hatch from egg in intestine (S.stercoralis ) or in

soil in (hook worms) .

Page 69: Specimen taken from feverish patient what is diagnosis.

Laboratory confirmation: A.lumbricoid, T.trichiura, and Hookworms is by finding eggs in feces and with S.stercoralis by finding larvae in stool.

E.vermicularis by scotch tape from skin around anus.

Some time worms of A.lumbricodis and E.vermicularis can be recovered in stool.

Page 70: Specimen taken from feverish patient what is diagnosis.

Feature of intestinal and tissue protozoa: Entamoeba histolytica, G.lamblia are motile

organisms that multiply and encyst in intestinal tract. they form cyst which excreted in faces. Invasive strains of

E histolytica multiply in intestinal wall. Cryptospordium multiply intracellular in cells.

It produces oocysts which are excreted in feces.

T.gondii muliply intracelluler in reticuloendothelial cell and cell of brain and other organs of body.

T.vaginalis is motile and multiplies in the urogenital tract cyst forms are unknown.

Page 71: Specimen taken from feverish patient what is diagnosis.

Infection is by ingesting cysts (E.histolytica, G.lamblia) or oocyst (Cryptosporidium,T.gondii) in food,water,or from hands

contaminated with infected feces. T.gondii can also be transmitted

congenitally and by ingesting the parasites in under-cooked meat of intermediate hosts.

T.vaginalis is transmitted sexually (no cyst).

Humans are important hosts of E. histolytica, G.lamblia and T.vaginalis.

Animal are natural definitive hosts of Cryptosporidium and T.gondii .

Page 72: Specimen taken from feverish patient what is diagnosis.

Laboratory confirmation of E.histolytica infection is by finding amoebae or cysts in feces or by detecting antibodies in serum (invasive amoebiasis)

Giardiasis diagnosed by finding motile flagellates or cysts in feces or flagellates in duodenal aspirates.

Infection with Cryptosporidium is diagnosed by finding oocyst in feces

Toxoplasmosis is usually diagnosed serologically.

T. vaginalis infection is usually confirmed by detecting flagellates in vaginal or uretheral discharge or urine

Page 73: Specimen taken from feverish patient what is diagnosis.
Page 74: Specimen taken from feverish patient what is diagnosis.

Pyrexia is not caused by:

1.Fascioliasis 2.Toxoplamosis3.Oxyuriasis 4.Kala-azar

Page 75: Specimen taken from feverish patient what is diagnosis.

The following parasites cause fever except:

1. Trichinella spiralis 2. Naegleria fowleri3. Hymenolepis nana4. Plasmodium vivax.

Page 76: Specimen taken from feverish patient what is diagnosis.

Infection with Giardia lamblia is through:

1. Ingestion of trophozoite stage

2. Ingestion of cyst stage3. Ingestion of oocyste4. Ingestion of eggs

Page 77: Specimen taken from feverish patient what is diagnosis.

Parasite causing duodenites is:

1. Giardia lamblia2. Entamoeba histolytica3. Toxoplasma4. Acanthamoeba

Page 78: Specimen taken from feverish patient what is diagnosis.

There is lymphadenopathy in following infection EXCEPT

1. African trypanosomiasis2. Toxoplasmosis 3. Schitosomiasis4. Kala-azar

Page 79: Specimen taken from feverish patient what is diagnosis.

Splenomegaly not caused by:

1. Schistosomaiasis2. Malaria3. Ascariasis4. Kala-azar

Page 80: Specimen taken from feverish patient what is diagnosis.

The following diseases occur as zoonoses:

1. Fasciolasis2. hydatid disease3. Toxoplasmosis4. African trypanosomiasis5. Cryptospordium6. Plasmodium vivax7. Leishmania major

Page 81: Specimen taken from feverish patient what is diagnosis.

For treatment of clinical attack of malaria we can use

1. 4-aminoquinolines (chloroquine)

2. Pentavalent antimonials3. 8-aminoquinoline (primaquine)4. All of above

Page 82: Specimen taken from feverish patient what is diagnosis.

For treatment of clinical relapse of P.vivax we can use

1. 4-aminoquinolines2. Pentavalent antimonials3. 8-aminoquinoline4. All of above

Page 83: Specimen taken from feverish patient what is diagnosis.

Duodenal aspirate is a good specimen for diagnosis of

1. Taeniasis2. Giardiasis 3. Amoebic dysentery4. Cysticercosis5. hydatidosis

Page 84: Specimen taken from feverish patient what is diagnosis.

Diagnosis by duodenal aspirateStrongyloides stercoralisFasciolasisCryptospordium pavarum.

Giardia lamblia ( enterotest)

Page 85: Specimen taken from feverish patient what is diagnosis.

Regarding Hymenolepis nana

1. Diagnosed by finding eggs in stools

2. Dwarf tapeworm3. Autoinfection can occur.4. Cattle act as intermediate

host

Page 86: Specimen taken from feverish patient what is diagnosis.

The following parasite is not transmitted by direct contact with infected person autoinfection :

1. Enterobius vermicularis.2. Trichella spiralis.3. Hymenolepis nana.4. Giardia lamblia.5. Entamoeba histolytica.6. Scabies (sarcoptes scabiei).

Page 87: Specimen taken from feverish patient what is diagnosis.

Splenomegaly is characteristic feature of one of following parasitic diseases:

1. Taeniasis 2. malaria3. Scabies 4. Myiasis5. Oriental sore.

Page 88: Specimen taken from feverish patient what is diagnosis.

Laboratory diagnosis of scabies is best done by

1.Serology2.Blood examination 3.Skin scraping4.Rectal biopsy5.Faecal examination.

Page 89: Specimen taken from feverish patient what is diagnosis.

Skin myiasis is due to invesion of skin by:

1. Sarcoptes scabiei2. Trematodes cercaria 3. Lice4. Fly larvae

Page 90: Specimen taken from feverish patient what is diagnosis.

The following correct regarding scabies

1. Transmitted by direct contact with infected person.

2. Caused by mites3. Affects skin4. Diagnosed by stools

examination

Page 91: Specimen taken from feverish patient what is diagnosis.

the following parasitic infection are water borne diseases except

1. taenia saginata2. madina worm3. Primary amoebic meningo-

encephalitis (Naegleria fowleri)4. Schistosoma5. Cryptospordium.

Page 92: Specimen taken from feverish patient what is diagnosis.

Acanthamoeba species cause1. Chronic primary

meningoencephalitis.2. Keratitis with blindness.

contact lense3. Granuloma of internal organs

and skin.4. All of above.5. Non of above.

Page 93: Specimen taken from feverish patient what is diagnosis.
Page 94: Specimen taken from feverish patient what is diagnosis.

Following diseases transmitted by an arthropod vector:

1. malaria2. Schistosomiasis3. cystocercosis4. Hydatid cyst.

Page 95: Specimen taken from feverish patient what is diagnosis.