Spatial Distribution of Copper and Iron in Cardiac Tissue
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Spatial Distribution of Copper and Iron in Cardiac Tissue Grant Allen
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Transcript of Spatial Distribution of Copper and Iron in Cardiac Tissue
Spatial Distribution
of Copper and Iron in
Cardiac Tissue
Grant Allen
Seminar Outline
Electron Probe Microanalysis
Nuclear Microscopy
Acknowledgements
Secondary Ion Mass Spectrometry
Conclusion
Introduction
Introduction
Investigate the spatial distribution of Cu and Fe in cardiac tissue
Analytical techniques:
Cardiac tissue that exhibits marked histological damage may
possess elevated levels of Cu and Fe
Electron probe x-ray microanalysis (EPMA)
Secondary ion mass spectrometry (SIMS)
Nuclear microscopy (NM)
Correlate topographical features with chemical composition
UHV techniques influence method of sample preparation
Electron Probe Microanalysis
Image courtesy of the Microscopy and Microanalysis Facility at the Department of Materials Engineering – Monash University
Detection limit in the region of 100 ppm
Primary ion beam: 5-20 kV electrons
Lateral resolution of 1 µm
Quantitative
Cryochamber
Specimen maintained at 80 K
Electron Probe Microanalysis
Energy /keV
Nuclear Microscopy
Rutherford Backscattering Spectroscopy (RBS) - normalisation
Scanning Transmission Ion Microscopy (STIM) - structural information
Particle Induced X-ray Emission (PIXE) - elemental analysis
Secondary electrons – complementary topographical information
Incident beam: 1.0-3.0 MeV H+ or He+
Lateral resolution of between 0.1 and 10 µm
Detection limit: ppb to ppm
Quantitative
UHV chamber
Specimen section freeze dried
Secondary Ion Mass Spectrometry
Image courtesy of the Bristol University CVD Diamond Group
Detection limit: ppb to ppm
Primary ion beam: 1-30 KeV 133Cs+
Non-quantitative analysis of biological specimens
Lateral resolution of 1 µm is possible
UHV chamber
Specimen section freeze dried
Secondary Ion Mass Spectrometry
Conclusion
Nuclear microscopy: provided the specimen is prepared in an
appropriate manner, determination of the spatial distribution of
metals in biological tissue is possible
Secondary ion mass spectrometry: non-quantitative
Electron probe x-ray microanalysis: insufficient sensitivity
Prof. Garth Cooper (Protemix)
Acknowledgements
Dr Anthony Phillips (Protemix)
Catherine Hobbis (School of Engineering - EPMA)
Dr Marcus Gustafsson (Department of Chemistry - SIMS)
Dr V. John Kennedy (Institute of Geological and Nuclear
Sciences - NM)
Dr Ritchie Sims (Department of Geology - EPMA)
