Southern Nevada Public Health Laboratory...
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Southern Nevada Public Health Laboratory (SNPHL)
Laboratory Update 2006Patricia Armour
Laboratory Manager
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SNPHL Update
State and Bioterrorism laboratory testing, facility and communication capability listed in this update were not available locally in Southern Nevada prior to 2004.
SNPHL is a branch of the State laboratory affiliated with the Southern Nevada Health District, through an interlocal agreement
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SNPHL - located in SNHD multi-use building
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SNPHL Three Laboratories in One
Bioterrorism testing
State laboratory testing
Clinical testing to support SNHD
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Bioterrorism testing at SNPHL
Only Southern Nevada laboratory registered with CDC to perform Laboratory Response Network (LRN) testingOnly Biological Safety Level 3 (BSL-3) containment laboratory in Southern NevadaAbility to test for at least 10 biological agents and toxins
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Biological Safety Level 3 (BSL 3) laboratory
Pass through decontamination autoclave BSL 3 lab interior
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SNPHL Organization and Staff
SNHD Community Health Service Division Dr. Larry Sands, DirectorDr. L D Brown – Director of SNPHL and Nevada State Health Laboratory-Reno1 Laboratory Manager, 6 Clinical Laboratory Scientists, 2 Laboratory Assistants, 2 Couriers
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Collaboration with local First Responders
Las Vegas Metropolitan Police DeptClark County and Las Vegas HazMatFederal Bureau of InvestigationUS Postal ServiceStandardize unknown sample collection protocols for submission of samples for biological testing
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State Laboratory Testing
Initiated and validated confirmatory testing for over 20 reportable disease isolates including Salmonella/Shigella serotyping and E. coli O157 confirmationReport confirmatory results to CDC for inclusion in national surveillance databases such as National Antimicrobial Resistance Monitoring System (NARMS)
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SNPHLMolecular testing (RNA/DNA)
Only local laboratory with capability to perform molecular testing for Norovirus and Avian Influenza
Current validation of molecular respiratory panel to be used for year round surveillance for Adenovirus, Influenza A/B, Parainfluenza
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Communication
Serve as resource for local laboratorians
Arrange testing and shipment of samples to CDC, FDA or other government lab
Provide courier service to local laboratories to ensure quick delivery of samples for confirmation testing
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Training
Provide local laboratories with training in identification of bioterrorism agents
Through CDC National Laboratory Training Network (NLTN), provide access to continuing education opportunities such as teleconferences
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Current Clinical Laboratory Testing
Perform clinical laboratory testing for Office of Epidemiology including stool culture and parasitology.Provide testing, sample collection instructions and courier pickup to the SNHD Outbreak Investigation TeamLead testing currently in validation
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Laboratory Information Management System (LIMS)
Provides electronictracking of specimens and result reportingconnectivity with Office of Epidemiologystorage of recordsinterface with automated analyzersfuture connectivity with CDC and SNHD Nursing Division
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Emergency Power
New emergency generator installed 9-16-06
Provides 100% backup power
for entire building
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Challenges
Continue validation of clinical laboratory testing for SNHD Nursing Division
LRN Recertification and CLIA Re-inspection
Staff must continue to be competent and able to perform testing in multiple departments: Microbiology, Molecular, and Immunology
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Challenges continuedSpace and Staff
Facility limitationCurrent laboratory space has reached maximum utilization for equipment and personnel. Space constraints will not allow for installation of new, unplanned for, equipmentWorkforce developmentDifficult to find licensed staff with high complexity research testing experience
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View of BSL-2 laboratory
Gas Chromatography analyzer for Microbial identification
Pulsed Field Gel Electrophoresis (PFGE)
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Challenges continuedProject Neon – MLK flyover
Desert Lane will become Martin Luther King Boulevard100% air intake at front of buildingConstruction may interfere with operation of BSL 3 laboratory and testing proceduresHigh traffic area may impact SNPHL security
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Front access to building on Desert Lane
Desert Lane