Application Cost Platform/Protocol Note

3’ mRNA-SeqCell capture/RT/library prep $1,990/Sample 10x Genomics

Chromium 500-10,000 cells/sample

5’ V(D)J + mRNACell capture/RT/library prep

(B and T cell enrichment)$2,460/Sample 10x Genomics

Chromium 500-10,000 cells/sample

Sequencing for 10x Genomics3’ single-cell library $1,990 for 350M reads* Illumina NovaSeq Custom 91bp sequencing

Sequencing for 10x Genomics3’ single-cell library $3,780 for 400M reads* Illumina NextSeq Custom 98bp sequencing

Sequencing for 10x Genomics5’ V(D)J + 5’ mRNA single-cell library $3,060 for 350M reads* Illumina NovaSeq 2x150bp sequencing

Full transcript single cell mRNA-SeqCell lysis/RT/QC

Library prep and QCSequencing (2M reads/cell)Sequencing (1M reads/cell)

$23/cell$10/cell$10/cell

$5.25/cell

SMART-Seq A minimum of2x96-well plates

* Price includes bioinformatics QC, alignment, quantification and visualization. For libraries generated by Genomics Core.

Single Cell Genomics

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Application Cost* Sample Minimum Sample Requirements Platform Read

TypeAverage Output

mRNA-Seq$349/Sample

$298/sample

6

> 40

RIN > 8500 ng – 1 µg NextSeq 1x75bp 25M reads

Ultra low-input mRNA-Seq $375/Sample 6 RIN > 8

50 ng – 400 ng NextSeq 1x75bp 25M reads

Total RNA-Seq $599/Sample 6 No mimimum RIN750 – 2 µg NextSeq 1x75bp 50M reads

RNA-Seq for FFPE $349/Sample 6DV200 > 30%

Total RNA: 50 – 400 ngFFPE: 250 – 500 ng

NextSeq 1x75bp 10M reads

Small RNA-Seq(UMI quantification) $375/Sample 6 RIN > 8 preferable

300 ng – 1.5 µg NextSeq 1x75bp 10M reads

*Price includes sample QC, library preparation, library QC, sequencing and basic bioinformatics quantification.

Next-Gen Sequencing-RNA

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Application Cost* Sample Minimum

SampleRequirements Platform Read

TypeAverage Output

Human Whole Genome

Sequencing (FFPE compatible)

$1,200/sample 61 -2 µg non-degraded gDNA

1 - 3 µg FFPE DNA; fragments > 1500 bp

NovaSeq 2x150bp 30X coverage

Human Whole Exome Sequencing(FFPE compatible)

$499/Sample

$449/sample

8

> 40

0.5 – 1 µg non-degradedgDNA

1 - 2 µg FFPE DNA; fragments > 1500 bp

NovaSeq 2x150bp 120X coverage

ATAC-Seq$489/sample

$829/sample8

5,000 – 50,000 cells in culture media; > 90%

viability

NextSeq

NovaSeq

2x75bp

2x50bp

2x40M reads

2x120M reads

16S Microbiome Amplicon-Seq $60/Sample** 50

10 µl/sample in an Eppendorf twin.tec plate

in columnsMiSeq 2x300bp 10K reads

ITS Mycobiome Amplicon-Seq $60/Sample** 50

10 µl/sample in an Eppendorf twin.tec plate

in columnsMiSeq 2x300bp 10K reads

*Price includes sample QC, library preparation, library QC, sequencing and basic bioinformatics quantification.** Price includes library preparation, pooled library QC and sequencing.

Next-Gen Sequencing-DNA

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Sequencing Platform Read Output Read type Cost*

NextSeq 400M 1x75bp $1,870/Sequencing run

NextSeq 2x130M 2x75bp $1,280/Sequencing run

NextSeq 2x400M 2x75bp $3,540/Sequencing run

NovaSeq 2x1300M 2x50bp $7,290/Sequencing run

NovaSeq 2x1300M 2x150bp $11,490/Sequencing run

NovaSeq 2x3300M 2x50bp $13,590/Sequencing run

NovaSeq 2x3300M 2x150bp $21,290/Sequencing run

MiSeq 2x15M 2x25bp $1,080/Sequencing run

MiSeq 2x25M 2x300bp $2,180/Sequencing run

*Only pooled libraries accepted. Full runs only (no shared runs). Price includes fragment analysis, fluorometric quantification, and sequencing of pooled library. One-time demultiplexing/FASTQ generation is included.

Sequencing Only

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Application Reaction Cost Platform

Gene expression 48 (targets*) x 48 (samples) $500/Plate Fluidigm BioMark HD

Gene expression 96 (targets*) x 96 (samples) $1,400/Plate Fluidigm BioMark HD

Genotyping 48 (targets*) x 48 (samples) $550/Plate Fluidigm BioMark HD

Genotyping 96 (targets*) x 96 (samples) $1,100/Plate Fluidigm BioMark HD

Human/rodent miRNA gene expression panel 745 (genes) x 3 (samples) $980/Plate QuantStudio 12K

*Primer pairs must be provided.

High-Throughout qPCR

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DNA Type Assay Concentration Range Size Range Cost Platform

dsDNA High Sensitivity 50 pg/µl – 5 ng/µl 25 – 5000 bp$20 for row + $5/sample

up to 12 samplesFragment Analyzer

dsDNA High Sensitivity 100 pg/µl – 10 ng/µl 50 – 7000 bp$60 for chip +

$5/sample up to 11 samples

Agilent Bioanalyzer

dsDNA Standard Sensitivity 5 ng/µl – 100 ng/µl25 – 5000 bp $20 for row + $5/sample

up to 12 samplesFragment Analyzer

dsDNA DNA 1000 0.5 ng/µl – 50 ng/µl 25– 1000 bp$20 for chip +

$5/sample up to 12 samples

Agilent Bioanalyzer

gDNA High Sensitivity 50 pg/µl – 5 ng/ml 50 – 40,000 bp$20 for row + $8/sample

up to 12 samplesFragment Analyzer

gDNA Standard Sensitivity 25 ng/µl – 250 ng/µl 75 – 48,500 bp$20 for row + $8/sample

up to 12 samplesFragment Analyzer

DNA Fragment Analysis &RNA Integrity

RNA Type Assay Concentration Range Cost Platform

Total RNA High Sensitivity 50 pg/µl – 5 ng/µl$20 for row + $5/sample up to

12 samplesFragment Analyzer

Total RNA RNA Pico 50 pg/µl – 5 ng/µl$60 for chip + $5/sample up to

11 samplesAgilent Bioanalyzer

Total RNA RNA Nano 5 – 500 ng/µl$20 for chip + $5/sample up to

12 samplesAgilent Bioanalyzer6

Assay Range Cost Platform

dsDNA High Sensitivity10 pg/µl – 100 ng/µl;

1-20 µl$6/sample Qubit

dsDNA Broad Range100 pg/µl – 1 µg/µl;

1-20 µl$6/sample Qubit

ssDNA*50 pg/µl – 200 ng/µl;

1-20 µl$6/sample Qubit

RNA High Sensitivity250 pg/µl – 100 ng/µl;

1-20 µl$6/sample Qubit

Fluorometric Quantification

*Also detects dsDNA and RNA. For accurate ssDNA concentration, the dsDNA and RNA assay kits must be used to detect dsDNA and RNA contamination.

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Application Type* Cost

Human cell line authentication** Genomic DNA $70/sample

Human cell line authentication** Dried cell pellet $90/sample

Human cell line authentication Analysis $25/sample

Mycoplasma contamination detection Genomic DNA $70/sample

Mycoplasma contamination detection Dried cell pellet $90/sample

* Inquire about fresh or frozen cells, or if you wish to do both Cell Line Authentication and Mycoplasma Contamination Detection on the same sample.** Human cell line authentication includes a mouse marker for cross-contamination detection.

Cell Line Authentication &Mycoplasma Contamination Detection

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Service Cost

RNA-Seq bioinformatics package$1,000 for first pairwise comparison for internal sequencing ($1,350 for external sequencing); $600 each for additional

comparisons

Custom bioinformatics service $125/hour; 8 hour minimum

Extra demultiplexing/FASTQ generation $60

Custom laboratory service $60/hour

Bioinformatics Analysis &Self-Use Equipment

Instrument Cost

ABI QuantStudio 12K Real-Time PCR System $20/hour

Eppendorf Vacuum Centrifuge $5/hour

Covaris M220 Focused Ultrasonicator $4/hour

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