CellAssist

Better Cell Imaging

Setting new standards for reproducible cell culture

CellAssist Imaging • Analytics • Documentation Reproducible Cell Culture

The CellAssist from Thrive Bioscience replaces your time at the inverted microscope to acquire, organize, and analyze images of all your cells in SBS format plates.

As a benchtop instrument, CellAssist automatically builds a database of images, metrics, and methods and materials to ensure centralized capture and retention of data. The unique CellAssist sets new standards for reproducible adherent and suspension cell culture and organoid culture, across numerous laboratory applications.

CellAssist Bench Top Imager in Lab

0.51m x 0.51m x 0.49m (lwh)

➋Insert plate into

CellAssist for imaging

OPTIMALOPTIMAL

➎Analyze, compare, and share images

➊Capture methods

and materials

➌Capture, view and

transmit to database

➍Remove plate for

continued workflow

EvalCore Analysis Workstation in Lab or Office

CellAssist Key FeaturesVessels and Environmental Control• Single plate imaging: 6- through 96-wells• Internal temperature: 37°C with CO2 option• Microscopy: 4x and 10x; phase contrast and bright-field

Imaging and Analytics

Data Input, Output and Networking

• Barcode reader documents plates, reagents, andprocesses via Bluetooth

• Tablet (Wi-Fi) in lab; workstation in office or lab; web browsers for multiple users

• Scans: full, center or defined regions of all or selectedwells

• Acquires hundreds of 5 megapixel images in minutes• Central database of images and metrics, including

growth rates, confluence, colony sizes, monoclonality• Captures images at up to 60 focal planes, each 5 μm

to 50 μm apart (user adjustable)

Centralized database provides consistent and shareable images to improve your cell culture experiments and results

• Organizes images across time, cell lines, and labs

• Images and displays cells at the same location on the cellculture plate within 15 µm across scans

• Generates extensive data sets of over 3 GB of images per scan

Objective metrics and quantitative reports enhance cell culture decision- making and productivity

• Characterizes cell dynamics, including growth curves, confluence,and the number and area of colonies

• Analyzes and compares cells and colonies to support decision- making to reduce variability and increase quality

• Allows comparison and optimization of methods and materials

Key workflow details and status of cells are captured and archived to provide confidence that data is not lost or compromised

• Accurately records and time-stamps your cell culture processeswith a barcode scanner

• Captures and archives the key workflow details and the status ofcells to provide confidence that data is not lost or compromised

• Enables comparison with previous experiments to optimize andensure reproducibility of your cell culture

CellAssist benefits for your laboratory

Imaging • Analytics • Documentation Reproducible Cell Culture

Analytics. Automation. Better Biology.

Cell culture withconfidence

Thrive Bioscience11 Audubon RoadWakefield, MA 01880 USA

Tel: 1-978-720-8044Email: info@thrivebio.comWebsite: www.thrivebio.com TM

CellAssist Customer Applications

The CellAssist Solution

• The CellAssist provides tools to efficientlyview and “grade” each colony based onuser-defined criteria

• Colony size measurement and confluencecurves characterize growth rates andcolony health

• Time-lapse images and videos assessthe monoclonality probability ofcandidate colonies

The CellAssist Solution

• The CellAssist objectively measuresconfluence of both the inner and outerregions of wells

• Confluence curves and time seriesimages are used to:

> compare and optimize processes

> identify outliers that are evidence ofprocess problems

> forecast optimal passaging times

Improves quality and increases yields by using data to grade and pick colonies

Objective metrics and images for consistent decision-making

Thrive Bioscience, , and CellAssist, are registered trademarks of Thrive Bioscience, Inc. EvalCore, Setting New Standards for Reproducible Cell Culture, and Imaging. Analytics. Documentation. Reproducible Cell Culture. are trademarks of Thrive Bioscience, Inc. The CellAssist is for in vitro and laboratory use only. Specifications are subject to change without notice. © Copyright 2021 Thrive Bioscience, Inc. All rights reserved.

TM

Control

Drug 1

Drug 2

Drug 3

Doubling Rate - Day

Wood (Breast) iPSCs (Patient A) BPEC (Breast) MSCs (Patient D) Stew (Pancreatic) Vero E6 HEK293T

Wood (Breast) iPSCs (Patient A) BPEC (Breast) MSCs (Patient D) Stew (Pancreatic)

200624 6well inspection B2Date scanned: 6/30/2020 1:08 PM Cell line: BPEC (Breast Cancer) Conditions: N/A Passage Number: 1 Plate Type: C-C 6Plate size: 6 wellsMagnifications: 4xIllumination Type: Phase ContrastConfluence: 23.20% Confluence Inner Ring: 18.70% Confluence Outer Ring: 26.50% Colony Count: 2102 Average Colony Area: 0.090 mm2 Average Grey Mean: 119.855 Average Grey Sigma: 6.772

Cell Lines

BPEC (Breast)

iPSCs (Patient A)

MSCs (Patient D)

St (Pancreatic)

Conditions

Control

Drug 1

Drug 2

Drug 3

Project: Drug Response

Confluence: 23.20%Confluence Inner Ring: 18.70%Confluence Outer Ring: 26.50%

Min. Area 0.1 +Min. Area 0.1 +

Max. Area 3000Max. Area 3000

Colony Count 131Colony Count 131

ID: 1 Area: 3.846ID: 1 Area: 3.846

ID: 2 Area: 3.846ID: 2 Area: 3.846

ID: 3 Area: 3.846ID: 3 Area: 3.846

ID: 4 Area: 3.846ID: 4 Area: 3.846

ID: 5 Area: 3.846ID: 5 Area: 3.846

ID: 6 Area: 3.846ID: 6 Area: 3.846

ID: 7 Area: 3.846ID: 7 Area: 3.846

ID: 8 Area: 3.846ID: 8 Area: 3.846

ID: 9 Area: 3.846ID: 9 Area: 3.846

ID: 10 Area: 3.846ID: 10 Area: 3.846

5-Good 5-Good

5 5

4 4

3 3

2 2

1-Bad 1-Bad

Scan Date: 2/8/2019 9:59 AMPlate size: 6 wellsMagnification: 4x

Illumination Type: Phase ContrastConfluence: 8.20%

Colony List

Colony Growth

Day 2 Day 6Day 4

Cell culture with confidence. Learn even more at: www.thrivebio.com

Thrive Bioscience, located in the Boston area, offers customers a family of instruments and software that provide imaging, analytics, and automation for reproducible cell culture.Our products empower biologists by combining advanced software, microscopy, androbotics, to acquire, organize, and analyze images of all their cells.

CA-BR_102020