Separation Science - Dongmyungdongmyung.co.kr › pds_home › broch › b489k.pdfAlltech...

Separation ScienceTODAY

a newsletter for today’s chromatographer

© Copyright 2004 Alltech Associates, Inc. UNP

Specialists in ChromatographySpecialists in Chromatography

SST VOLUME 4 brochure #489K

Page 12 & 15

I N S I D E

INDUSTRY FOCUS:PharmaceuticalPages ............................... 9

General InterestPages ............................. 13

PRODUCT INFO:HPLC/ICPages .................... 3-11, 16

GCPages ........................ 12-14

Sample HandlingPages ........................ 14-15

2

INDUSTRY FOCUSPharmaceuticalSeparation of a Dissimilar Complex Antibiotic Pair Using

a New Reversed-Phase HPLC Column and ELSD 9

General InterestOnsite Generation of Fuel Gases for Flame

Ionization GC Detectors 13

HPLC/ICNew! Alltima™ HP HPLC ColumnsHigh performance from high purity silica 3

Alltima HP HPLC Column Applications 4

ProSphere™ HPLC ColumnsHPLC columns for the life science industry 5

Astec CHIROBIOTIC™ HPLC ColumnsSimplify your most challenging chiral separations 6

Alltech's Method Development Assistant SoftwareMeet your new assistant 7

Alltech Degassing SystemsThe most advanced degassing technology 8

Model 2000 and Model 800 ELSDSensitive, universal HPLC detection 8

Ion Chromatography Starter KitsSave money with starter kits 10

New! Ion SuppressorsOutlast and outperform membrane suppressors 11

SofGrip™ HPLC FittingsThe toughest fitting with the softest grip 16

GCNew! Parker Balston® Model FID-1000 Gas StationHydrogen and zero air from one gas generator 12

Alltech Indicating Hydrocarbon TrapSee for yourself - the only indicating hydrocarbon traps 12

Alltech Digital Flow Check™ Mass FlowmetersA better way to measure gas flow 14

SAMPLE HANDLINGDrug-Clean™ SPE ColumnsGet more accurate drug analysis results 14

Autosampler VialsTake advantage of our special pricing 15

In This Issue:

3www.dongmyung.co.kr

New! High performance from high purity silica

Degassing Systems SpecificationsPhase Particle Size Carbon Loading Pore Size Surface Area End Capped Position

C18 3 and 5µm 12% 190Å 200m2/g Yes Most popular phase

C8 3 and 5µm 8% 190Å 200m2/g Yes Faster peak elution

C18 HiLoad 3 and 5µm 24% 100Å 450m2/g Yes More retentive for diffi cult separations

EPS C18 3 and 5µm 4% 190Å 200m2/g No Unique polar selectivity

C18 Amide 3 and 5µm 12% 190Å 200m2/g Yes Low bleed MS applications: 100% aqueous

compatibility

Cyano 3 and 5µm 4% 190Å 200m2/g Yes Unique retention and stability

Silica 3 and 5µm - 100Å 450m2/g No Normal phase

AlltimaTM HP HPLC ColumnsC18 C18 EPS

PARTICLE C18 C8 HiLoad Amide C18 Cyano SilicaSIZE LENGTH X ID PART NO. PART NO. PART NO. PART NO. PART NO. PART NO. PART NO.

Analytical 3µm 100 x 4.6mm 87667 87739 87685 87721 87703 87757 877853µm 100 x 2.1mm 87669 87741 87687 87723 87705 87759 87787

3µm 150 x 4.6mm 87668 87740 87686 87722 87704 87758 87786

3µm 150 x 2.1mm 87670 87742 87688 87724 87706 87760 87788

5µm 150 x 4.6mm 87679 87751 87697 87733 87715 87769 87797

5µm 150 x 2.1mm 87681 87753 87699 87735 87717 87781 87799

5µm 250 x 4.6mm 87680 87752 87698 87734 87716 87780 87798

5µm 250 x 2.1mm 87682 87754 87700 87736 87718 87782 87802

Rocket™ 3µm 33 x 7mm 87671 87743 87689 87725 87707 87761 877893µm 53 x 7mm 87672 87744 87690 87726 87708 87762 87790

Expedite™ 3µm 10 x 2.1mm 87673 87745 87691 87727 87709 87763 877913µm 10 x 4.6mm 87675 87747 87693 87729 87711 87765 87793

3µm 20 x 2.1mm 87674 87746 87692 87728 87710 87764 87792

3µm 20 x 4.6mm 87676 87748 87694 87730 87712 87766 87794

4

Alltima HP Application

Peak Identification1. Methionine Enkephalin2. Leucine Enkephalin3. Angiotensin II4. Neurotensin5. Angiotensin I6. Substance P

Peptide Mix

Column: Alltima HP C18, 5µ, 250 x 4.6mm Mobile Phase: A: 0.1% TFA in water / B: 0.08% TFA in

acetonitrile Gradient: (Time,%B),(0,20),(20,40) Flow Rate: 1.0mL/min Detector: UV at 220nm

10369

Peak Identification1. Desipramine (0.05 mg/mL)2. Nortriptyline (0.05 mg/mL)3. Diphenhydramine (0.25 mg/mL)4. Amitriptyline (0.05 mg/mL)

Tricyclic Antidepressants

Column: Alltima HP C8, 5µ, 150 x 4.6mm Mobile Phase: A: 25mM Phosphate Buffer pH7 / B: Methanol Gradient: (Time,%B),(0,60),(8,75),(20,75) Flow Rate: 1.0mL/min Detector: UV at 254nm

10373

Peak Identification1. Sulfanilamide2. Sulfamerazine3. Sulfamethazine4. Sulfamethizole

Sulfa Drugs

Column: Alltima HP C18 HiLoad, 5µ, 150 x 4.6mm Mobile Phase: (85:15) 0.1% phosphoric acid:acetonitrile Flow Rate: 1 mL/min Detector: UV at 254nm

10351

Peak Identification1. Ethotoin2. Phenytoin3. Mephenytoin4. Carbamazepine

Anticonvulsants

Column: Alltima HP C18 HiLoad, 5µ, 150 x 4.6mm Mobile Phase: (70:30) 25mM ammonium acetate:acetonitrile Flow Rate: 1 mL/min Detector: ELSD

10349

Peak Identification1. Cytidine2. Uridine3. Xanthosine4. Guanosine5. Adenosine

Nucleosides

Column: Alltima HP EPS C18, 5µ, 150 x 4.6mm Mobile Phase: A: 0.03M potassium phosphate, pH3.2 /

B: acetonitrile Gradient: (Time,%B),(0,5),(2,5),(20,30) Flow Rate: 1 mL/min Detector: UV at 260nm

10347

Peak Identification1. Nortriptyline2. Diphenhydramine3. Amitriptyline

Basic Drugs

Column: Alltima HP C18 HiLoad, 5µ, 150 x 4.6mm Mobile Phase: (85:15) methanol:25mM potassium phosphate pH 7 Flow Rate: 1mL/min Detector: UV at 254nm

10352


Alltech ProSphere™ HPLC Columns

Other column dimensions are available. Visit our web store at www.alltechWEB.com or call your local Alltech office.i

ProsphereTM HPLC ColumnsDESCRIPTION LENGTH X ID PART NO.

ProsphereTM C18 150 x 150µm 32904300Å 250 x 150µm 329055µm 150 x 300µm 32907

250 x 300µm 32908

150 x 2.1mm 35098

150 x 4.6mm 32969

250 x 4.6mm 32971


250 x 300µm 33908

150 x 2.1mm 35096

150 x 4.6mm 33989

250 x 4.6mm 33991


250 x 300µm 35508

150 x 2.1mm 35090

150 x 4.6mm 35091

250 x 4.6mm 35087

Request a FREE copy of our new ProSphereTM Life Science Catalog

Introducing a full line of Size Exclusion, Ion Exchange, Affinity, Hydrophobic Interaction,and Reversed-Phase Chromatography Columns. To receive a catalog, use the insert,request on-line at www.alltechWEB.com, or call 1-800-ALLTECH.

6

For additional information on chiral separations, visit our online technical library at www.alltechWEB.com.i

Separation of Metoprolol on CHIROBIOTICTM T

Separation of Bupivacaine on CHIROBIOTICTM V

Methanol with Ammonium Trifl uoroacetate at 0.1% by weight1.0mL/min

Methanol with Ammonium Trifl uoroacetate at 0.1% by weight1.0mL/min

Astec CHIROBIOTIC™ HPLC Columns

CHIROBIOTICTM V: amines; including cyclic and tertiary amines, esters, neutral molecules and profens

CHIROBIOTICTM T: -halogenated aliphatic acids, amino acids, beta-blockers, carboxylic acids, cyclic aromatics, neutral aromatics, and small peptides

CHIROBIOTICTM R: -hydroxy acids, anionic molecules, aromatic esters, alcohols, di- and tri-peptides, and substituted -halogenated acids

CHIROBIOTICTM TAG: amino acids, beta-amino acids, carnitine, courmarins, diazopines, hydantoins, oxazolidinones, phosphonates, small peptides, and sulfoxides

FREEChiral MethodDevelopment

Wall Chart with Purchase!

Use Promo Code SP035

Peak 1 - 15.4min.Peak 2 - 17.1min.

Peak 1 - 5.6min.Peak 2 - 6.2min.

Astec CHIROBIOTICTM HPLC ColumnsCHIROBIOTICTM V CHIROBIOTICTM T CHIROBIOTICTM R CHIROBIOTICTM TAG

LENGTH X ID PART NO. PART NO. PART NO. PART NO.

100 x 4.6mm 11022AC 12022AC 13022AC 14022AC

150 x 4.6mm 11023 12051 12516 14023AC

250 x 4.6mm 11024 12053 12518 14024AC

Guards Cartridge, 20 x 4.0mm 11019 12445 12522 14100AC

Holder 11014 11014 11014 11014


New! Alltech’s Method Development Assistant Software

Your Assistant Helps Develop New Methods

Column kit includes:

150 x 4.6mm, 5µm columns from our premium, and best selling product lines.- PrevailTM C18- PrevailTM Select C18- AlltimaTM HP EPS- AlltimaTM HP C18 HiLoad- AlltimaTM HP Amide

FREE Column Kit!

Your Assistant Gets Existing Methods to Work

Meet Your New Assistant . . .

Method Development ToolsDESCRIPTION PART NO. PRICE

Method Development ToolsAnalytical Column Kit 22345 2500

Software Alone 22348 3118.75

Software and FREE Column Kit 22347 3118.75

For more information on Alltech's MDATM, visit our online technicallibrary or call for a demo or quote.i

8

Degassing Systems and ELSDsLC Instruments to Address Specific Needs and Budgets

The most advanced degassing technology

Alltech On-line Degassing Systems offer:

Sensitive, universal HPLC detection

Model 2000 ELSD Model 800 ELSD

For information on the ELSDs, visit our online technical library, or call and request brochures 474 and 475.For information on the Degassing Systems, visit our online technical library, or call and request brochure 479.i

Separation of a Dissimilar Complex Antibiotic Pair Using a New Highly Versatile Reversed-Phase HPLC Column and ELSDRomulus Gaita and Dr. Cary Thrall, [email protected]

Industry Focus - HPLCpharmaceutical


See page 8 for ELSD information. For information on PrevailTM Select C18, visit our online technical library at www.alltechWEB.com, or call and request document M076.i

1. Tylosin2. Monensin

Figure 2

Column: PrevailTM Select C18, 5µm, 150 x 4.6mm (Part No. 99300)

Mobile Phase: 95:5 Acetonitrile:Wateradjusted to pH 9 w/NH4OH

Flowrate: 1.0mL/minDetector: ELSD 2000 N2 Flow: 2.5L/min Drift Tube: 75°C, Impactor Off

Figure 1a - Monensin

Figure 1b - Tylosin

Figure 3

10

Ion Chromatography Starter Kits

U.S. EPA Methods Anion Column Kit - for EPA Methods 300.0, 300.1, 317.0. and 326.0

Includes a NovosepTM A-2 Column (250 x 4.0mm), guard cartridges, a 3.6mM carbonate EZ-LUTETM Concentrate, and anion and oxyhalide standards.

Suppressor-Based Anion Column Kit - for analysis of anions by suppressor-based methods

Includes a NovosepTM A-1 Column (150 x 4.6mm), guard cartridges, a 1.7mM/1.8mM bicarbonate/carbonate EZ-LUTETM Concentrate, and anion standards.

Non-Suppressed Anion Column Kit - for analysis of anions by non-suppressed methodsIncludes an AllsepTM Anion Column (100 x 4.6mm), guard cartridges, a 4mM pHBA EZ-LUTETM Concentrate, and anion standards.

Mono- and Divalent Cation Column Kit - for simultaneous analysis of mono- and divalent cations

Includes a Universal Cation Column (100 x 4.6mm), guard cartridges, a 3mM methanesulfonic acid EZ-LUTETM Concentrate, and cation standards.

IC Accessory Kit - tools and accessories for every IC systemIncludes PEEK tubing, one-piece fi ttings, a PEEK union, two different size plastic syringes, a luer-hub needle, tubing cutter, wrench, and two different in-line solvent fi lters.

Request a FREE copy of our new Ion Chromatography brochure

Alltech’s new Ion Chromatography Brochure includes all the products you need toperform high-quality ion chromatography separations. Learn about our new UniversalCation HR Columns and our new low-cost, high-sensitivity suppressors. Browse ourcomplete line of IC standards, including new standards for amines and organic acids.Choose Alltech for your IC needs, and receive the friendly, helpful customer support youexpect and deserve. To receive a brochure, use the insert, request on-line atwww.alltechWEB.com, or call 1-800-ALLTECH .

IC Starter KitsDESCRIPTION PART NO.

U.S. EPA Methods Anion Column Kit 270107

Suppressor-Based Anion Column Kit 270106

Non-Suppressed Anion Column Kit 270102

Mono- and Divalent Cation Column Kit 269105

IC Accessory Kit 269101

Kit includes a collection of tubing, fi ttings, and other

hardware.

Kits include an analyticalcolumn, guard columns,buffer concentrate, and

standards.

Accessory Kit Column Kits


New! IC Suppressors

Alltech SuppressorsDESCRIPTION CAPACITY PART NO.

Model 640 Suppressor (Isocratic)120V 20mN Sodium 640100

240V 20mN Sodium 640200

Model 641 Suppressor (High Sensitivity and Gradient)120V 40mN Sodium 641100

240V 40mN Sodium 641200

For information on the Alltech Suppressors, visit ouronline technical library at www.alltechWEB.com, orcall and request brochure 484.i

1. Methionine Enkephalin2. Leucine Enkephalin

1. Methionine Enkephalin2. Leucine Enkephalin

Peptide Mix

Peptide Mix

Peptide Mix

Column: Alltima HP C18, 5µ, 250 x 4.6mm Mobile Phase: A: 0.1% TFA in water / B: 0.08% TFA in

acetonitrile Gradient: (Time,%B),(0,20),(20,40) Flow Rate: 1.0mL/min Detector: UV at 220nm

9290

9291

Only Alltech’s suppressors degas the effl uent for high sensitivity,stable baselines, and better resolving power.

* U.S. patent numbers 6,444,475; 6,468,804

12

AT™-Indicating Hydrocarbon Traps AT™-Indicating Hydrocarbon In-Line Trap

AT™-Indicating HydrocarbonCartridge Trap

AT™-Indicating Hydrocarbon TrapsDESCRIPTION PART NO.

In-Line Trap, 1/8" fittings 7210

In-Line Trap, 1/4" fittings 7212

Cartridge Trap (requires baseplate) 81017

Cartridge Baseplate 81025

New! Parker Balston Model FID-1000 Gas Station

Model FID-1000 Gas Station SpecificationsGAS PRESSURE PURITY FLOW

Hydrogen: 0-90psig 99.9995% 90cc/min

Zero Air: 0-60psig


Onsite Generation of Fuel Gases for Flame Ionization GC DetectorsReggie Bartram, [email protected]

Abstract

Introduction

Conclusion

References1. Compressed Gas Association, Inc. Handbook

of compressed gases, New York: Van Nostrand Reinhold Co., 1981

2. Robert Daly, The Shift to On-Site Gas Generation, American EnvironmentalLaboratory, March 1995

3. Air Products and Chemicals, Inc. Safety-gram-14. Don’t turn a cylinder into a rocket: 1988.

4. Albert A, et al. What happens to gas cylinder purity as content is consumed? IEEE/SEMI Advanced Semiconductor Manufacturing Conference, 1993, Boston, MA

Industry Focus - GC

Baseline Comparison

general interest

14

Alltech Digital Flow Check™ Mass Flowmeters

New! Mixed-Mode Drug-Clean™ SPE ColumnsGet more accurate drug analysisresults

Model DFC-HRTM

Digital Flow CheckTM Flowmeters Specifi cationsFlow range: DFCTM 0.1-500 SCCM (except CO2 to 300 SCCM)

DFC-HRTM 0.01-500 SCCM (except CO2 to 300 SCCM)

Accuracy: +/-2% of reading Display: 16-character alphanumeric LCD, autoranging

Display modes: mass fl ow, split ratio, linear velocity (std. fl ow range)

Power: 9V alkaline battery (included), auto-off function,

low battery warning

Dimensions: 190.5 x 57.4 x 101.6mm (7.5" x 2.26" x 4.0")

For a complete list of drug standards,visit our online technical library atwww.alltechWEB.com.i

Digital Flow CheckTM FlowmetersMODEL PART NO.

DFCTM 4700

DFC-HRTM 5700

Mixed Mode Drug-CleanTM ColumnsDESCRIPTION QTY PART NO.

200mg/4mL 50 207010

Cocaine and Benzoylecgonine Extract from Urine

1. Cocaine (2.5 mg/ml) 2. Benzoylecgonine-TMS,

Benzoylecgonine-d3-TMS (5.0mg/mL)

Column: Helifl ex® AT-5ms, 30m x 0.25mm x 0.25µm (Part No. 15807)

Temp: 225°C (0 min hold) to 315°C (11 min hold) at 10°C/min

Carrier: Helium at 0.8mL/min (28cm/sec)

Detector: MSD at 325°C

2656


Alltech Autosampler Vials

Convenience Kits Ordering InformationLINER DESCRIPTION TFE/BUTYL LINER TFE/SILICONE LINER TFE/SILICONE/TFE LINER

VIAL AND CLOSURE DESCRIPTION KIT PART NO. KIT PART NO. KIT PART NO.

12x32mm Crimp Style Vials, 11mm SealsStandard Mouth Crimp Vials, Aluminum Seals 95161 98318 98373

WIDE Mouth Crimp Vials, Aluminum Seals 98397 98419 98447

Snap Seal Vials, Clear Poly CrimpTM Seals 88487 88496 88494

12x32mm Clear Screw Thread VialsStandard Mouth Vials, 8/425 Black Cap 98380* 89074 89078

WIDE Mouth Vials, 10/425 Black Cap 97243 97239 97252

Robotic R.A.M.TM‚ Vials, 9mm Black Cap 89135 89145 89149

* Preassembled kits only

For more information on vials, visit our online technical library at www.alltechWEB.com, or call and request brochure 445.i

Screw Thread Vials and Caps

Kits contain: Unassembled, 100 each Clear Glass Vials, Caps with Liners

Crimp Vials with Aluminum Seals Snap Seal Vials with Poly CrimpTM Seals

Q:

A:

SofGrip™ HPLC Fittings

www.alltechWEB.com

01/0

4 #

489K

© Copyright 2003 Alltech Associates, Inc.Alltech reserves the right to change prices and/or specifications without prior notification.

Use of Our Products: Alltech Associates, Inc., supplies chromatographic equipment reagents and accessories for non-medical

laboratory use only. This means our products: ... may NOT be used as drugs, ...may NOT be used as cosmetics, ...may NOT be

used as medical devices or as in vitro diagnostic devices.

Printed in the USA.

SofGripTM One-Piece FittingsSTANDARD NO-SLIPTM

COLOR QTY PART NO. PART NO.

Black, Short 10 40501 40511

Black, Long 10 40506 40516

Red, Short 10 40502 40512

Blue, Short 10 40503 40513

Green, Short 10 40504 40514

Yellow, Short 10 40505 40515

Assortment, Short 10 40507 40517

OtherFittings

No-SlipTM

Fitting

The trademarks referred to herein are the property of their respective owners.

Separation Science - Dongmyungdongmyung.co.kr › pds_home › broch › b489k.pdfAlltech...

Documents

Transcript of Separation Science - Dongmyungdongmyung.co.kr › pds_home › broch › b489k.pdfAlltech...