Science and Probiotics and IBS · PDF file•Lead for clinical research at Protexin ......
Transcript of Science and Probiotics and IBS · PDF file•Lead for clinical research at Protexin ......
Science
and nature
in balance
My background
• Dr Ashton Harper MBBS, iBSc
(Phys/Pharm), MRCS
• MD from University College London
School of Medicine
• 5 years clinical experience in medical
and surgical disciplines
• Special interest in gastrointestinal
diseases
• Head of Medical Affairs at the UK’s
leading probiotic manufacturer
• Lead for clinical research at Protexin
• Facilitate the understanding & use of
probiotics in clinical medicine
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Clinical outcomes - IBS
• 1793 patients were included from 24 clinical trials
• Abdominal pain score RR of responders probiotics vs placebo
= 1.96 (P = 0.01)
• Global symptom score RR of responders probiotics vs placebo
= 2.43 (P = 0.02)
• Adequate improvement of general symptoms in IBS RR
probiotics vs placebo was 2.14 (P = 0.03)
• ‘Probiotics reduce pain and symptom severity scores. The
results demonstrate the beneficial effects of probiotics in IBS
patients in comparison with placebo.’
• 3406 patients – 9 SRs and 35 RCTs
• 29 dose specific probiotic formulations
• 16 single strain RCTs
• 19 multi-strain RCTs
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McKenzie et al 2016 - Results
• 83% of RCTs improved at least 1 outcome measured
• Global symptoms –14 of 29 trials found a ‘statistically significant improvement’
• 65% multi-strain
• 35% single strain
• ‘Clinically meaningful improvement in abdominal pain’ – 8 of 35 trials
• 63% multi-strain trials
• 37% single strain trials
• QoL - 16/35 measured
• Only 2 of 16 found ‘clinically meaningful improvement
• 100% multi-strain
• No reported ‘serious adverse events’
• GI AE in 1 RCT
Supplementation with a multistrain probiotic formulation (Bio-Kult®) in the management of Irritable Bowel Syndrome - a randomized, double-blind, placebo-controlled clinical trial (Completed 2017 - awaiting publication)
• Male and female patients 18-55yrs
• Rome III diagnosis of moderate-severe IBS-D
• Exclusion of non-functional GI disease
• 4 months of intervention
• Monthly follow-up – final at 5 months
• 2 capsules BD = 8 billion CFU/day of multi-strain formula (or cellulose placebo capsules with identical appearance)
2 billion microorganisms per capsule
(1x1010 CFU/gram)
Guaranteed 2 year shelf-life
14 strains of live bacteria
No refrigeration required
Bio-Kult® Advanced
•Bifidobacterium bifidum PXN 23
•Bifidobacterium breve PXN 25
•Bifidobacterium infantis PXN 27
•Bifidobacterium longum PXN 30
•Lactobacillus acidophilus PXN 35
•Lactobacillus delbrueckii ssp. bulgaricus PXN 39
•Lactobacillus casei PXN 37
•Lactobacillus plantarum PXN 47
•Lactobacillus rhamnosus PXN 54
•Lactobacillus helveticus PXN 45
•Lactobacillus salivarius PXN 57
•Bacillus subtilis PXN 21
•Lactococcus lactis ssp. lactis PXN 63
•Streptococcus thermophilus PXN 66
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Outcome measures and sample size
• Primary outcome:
• Abdominal pain (IBS-SSS)
• Secondary outcomes:
• Global symptom severity (IBS-SSS)
• QoL (IBS-QoL)
• 400 patients randomised:
• Minimum clinically important difference = 30% (based on SD from G. Sisson et al 2013) and accounting for attrition the adjusted sample size = 400
NB: mainly small and underpowered studies:
• Only 10/35 had total sample size >100
• Only 3/35 >100 per arm
• Therefore ~75% were pilot studies
Science
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Assessing IBS symptoms
IBS-Severity Scoring System (IBS-SSS):
• 5 questions
• Covers: pain (x2), distension/bloating, bowel habit satisfaction and interference with life.
IBS-Quality of Life (IBS-QoL) questionnaire:
• 34 items, each with a five-point response scale (1. Not at all 2. Slightly 3. Moderately 4. Quite a bit 5. Extremely)
• Cover 8 domains: Dysphoria, Interference with Activity, Body Image, Health Worry, Food Avoidance, Social Reaction, Sexual, Relationships
IBS pain results • IBS pain severity decreased by 69% in the
probiotic arm
NB: IBS pain frequency decreased by >70% (7.7 per 10 days to 2.2 at month 5 vs 3.9 from 8.1 in placebo)
• Internationally agreed symptom outcome measures are lacking!
Establishing clinically meaningful outcomes in IBS
NB: placebo effect is 37.5% in pharmaceutical RCTs in IBS
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Further results
IBS-SSS:
• Significant benefit of probiotic over placebo at all time points
• Reduced by 145 points within 30 days (vs. 117 points in the placebo group)
• Reduced by 223 points by month 5 (vs. 157 points in the placebo group).
Severity (moderate to severe) at end of follow-up:
• <14% probiotic
• 48% placebo
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Conclusions
• Multi-strain probiotics have the most frequent success in IBS management
• Protexin have conducted the largest IBS-D probiotic trial to date
• If you have moderate to severe IBS-D taking Bio-Kult could:
• Improve the severity and frequency of your pain by ~70%
• Decrease distension by 2/3rds
• Cut trips to the loo by >50%
• Significantly boost your QoL
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References
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2. Sender, R., Fuchs, S. & Milo, R. Are we really vastly outnumbered? Revisiting the ratio of bacterial to host cells in humans. Cell 164, 337–340 (2016).
3. Lozupone CA, Stombaugh JI, Gordon JI, Jansson JK, Knight R. Diversity, stability and resilience of the human gut mi- crobiota. Nature 2012; 489: 220-230 [PMID: 22972295 DOI: 10.1038/nature11550]
4. Qin, J. et al. A human gut microbial gene catalogue established by metagenomic sequencing. Nature 464, 59–65 (2010).
5. Clemente JC, Pehrsson EC, Blaser MJ, Sandhu K, Gao Z, Wang B, Magris M, Hidalgo G, Contreras M, Noya-Alarco´ n O´ et al.: The microbiome of uncontacted Amerindians. Sci. Adv. 2015, 1: e1500183.
6. Lacy BE et al. Bowel Disorders. Gastroenterology 2016; 150:1393-1407
7. Matricon J. Review article: associations between immune activation, intestinal permeability and the irritable bowel syndrome. AP&T 2012. 36: 1009–1031
8. Kennedy PJ. Et al. Irritable bowel syndrome: A microbiome-gut-brain axis disorder? World J Gastroenterol 2014 October 21; 20(39): 14105-14125
9. Parkes GC, Rayment NB, Hudspith BN, et al. Distinct microbial populations exist in the mucosa-associated microbiota of sub-groups of irritable bowel syndrome. Neurogastroenterol Motil 2012; 24:31–9.
10. Rigsbee L, Agans R, Shankar V, et al. Quantitative profiling of gut microbiota of children with diarrhea-predominant irritable bowel syndrome. Am J Gastroenterol 2012; 107: 1740–51.
11. Jalanka-Tuovinen J, Salojarvi J, Salonen A, et al. Faecal microbiota composition and host-microbe cross-talk following gastroenteritis and in postinfectious irritable bowel syndrome. Gut 2013; doi: 10.1136/gutjnl-2013-305994 [Epub ahead of print].
12. Straudacher HM. Mechanisms and efficacy of dietary FODMAP restriction in IBS. Nature Reviews Gastroenterology & Hepatology 11, 256–266 (2014)
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References continued
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5. Crouzet L, Gaultier E, Del’Homme C, Cartier C, Delmas E, Dapoigny M, Fioramonti J, Bernalier-Donadille A. The hypersensitivity to colonic distension of IBS patients can be transferred to rats through their fecal microbiota. Neurogas- troenterol Motil 2013; 25: e272-e282 [PMID: 23433203 DOI: 10.1111/nmo.12103]
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