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SALMONELLA TYPHIMURIUM- GETTING TO THE BOTTOM OF IT G. Doran, N. DeLappe, C. O Hare, G. Corbett –...
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Transcript of SALMONELLA TYPHIMURIUM- GETTING TO THE BOTTOM OF IT G. Doran, N. DeLappe, C. O Hare, G. Corbett –...
SALMONELLA SALMONELLA TYPHIMURIUM- GETTING TYPHIMURIUM- GETTING TO THE BOTTOM OF ITTO THE BOTTOM OF IT
G. Doran , N. DeLappe,
C. O Hare, G. Corbett – Feeney,
M. Cormican
National Salmonella Reference Laboratory, Galway
INTRODUCTIONINTRODUCTIONS. TYPHIMURIUM COMMON IN
EUROPE, USA
POULTRY, CATTLE & PIGS MANY FOODS
MULTIDRUG RESISTANT ISOLATES
INTRODUCTIONINTRODUCTION -2 -2 MULTI DRUG RESISTANT
DT104
INCIDENCE INCREASING ACSSuT – CHROMOSOMALLY
CARRIED TRIMETHOPRIM, NALIDIXIC
ACID RESISTANCE SUSCEPTIBILITY TO
CIPROFLOXACIN
TYPING METHODSTYPING METHODSPHAGE TYPING
ANTIMICROBIAL SUSCEPTIBILITY
PLASMID PROFILING
PFGE
PRINCIPLES OF PFGEPRINCIPLES OF PFGE
CATTTTACGTAAAATG
PRINCIPLES OF PFGEPRINCIPLES OF PFGE
CATGTTACGTACAATG
NO CUT
PULSENETPULSENETSTANDARDISED PFGE
CDC USA
E. coli, Salmonella, Listeria, Shigella
CENTRAL DATABASE
PFGEPFGE
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
PLASMIDPLASMID PROFILE PROFILE1 2 3 4 5 C C C C 6 7 8 9 10
Size (MDa)
125
26
10
2
SS. TYPHIMURIUM 2001. TYPHIMURIUM 2001
303 ISOLATESANIMAL AND HUMAN ORIGIN
PHAGE TYPING (N = 303)ANTIMICROBIAL SUSCEPTIBILITY
(N = 303)PGFE TYPING (N = 163)PLASMID PROFILING (N = 36)
S. Typhimurium Phage Types
DT104
DT104b
DT193
U310
U302
DT132
Other
ANTIMICROBIAL ANTIMICROBIAL SUSCEPTIBILITYSUSCEPTIBILITY
0
20
40
60
80
100
1 2
Resistance Pattern
% R
esis
tanc
e
Non MDR
Other MDR
ASSuT
ACSSuTSp +
ACSSuTSp
PLASMID PROFILINGPLASMID PROFILING36 ISOLATESMODIFIED METHOD OF KADO
AND LIU
PLASMIDS 1.5 - 125 MDA60MDA PLASMID IN DT104, MDR
DT120 AND DT12
A
B
C
D
E
F
G
H
MDRPATTERN PHAGE TYPE
DT104, DT104b, MDR DT12 &12088% MDR
32 ISOLATESFULLY SENS
DT12
DT120
PHAGE TYPE PHAGE TYPE U310U310
EMERGED IN 2001 PIGS, HUMANS15 ISOLATES MINOR DIFFERENCES
ON PFGE
DISCUSSIONDISCUSSION S. TYPHIMURIUM –
HUMANS & ANIMALS MDR - INCREASED
DT104 & DT104b MAJORITY MDR, CLONAL
DISCUSSION - 2DISCUSSION - 2 DT104, DT104b
PFGE U302, RDNC
P11 MDR DT12, DT120
P3, P5,P7, P14 DT193P18, P20P23, P27
CONCLUSIONSCONCLUSIONSPHENOTYPIC & GENOTYPIC
METHODS NECESSARY
HIGH INCIDENCE OF MDR IN IRELAND
CONTINUOUS MONITORING OF S. TYPHIMURIUM NECESSARY
OTHER RESEARCHOTHER RESEARCH
FLUROQUINOLONE RESISTANCE IN S. ENTERITIDIS– DONNA KILMARTIN