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Jibin Abraham Punnoose

Department of Chemistry and Biochemistry, Kent State University, Kent, OH 44242 Phone: (330)-687-9251; Email id: jabraha4@kent.edu

https://www.linkedin.com/in/jibin-abraham-punnoose-77b761a6

Professional Summary

I am a doctoral student in the final semester of my research with expertise in molecular biology,

bioanalytical methods and single-molecule techniques.

Skills include DOE, bioanalytical method development and validation, data analysis and handling

common analytical chemistry instrumentation.

Looking forward to a challenging R&D career in a leading biotech company where I can contribute

to interdisciplinary discovery teams working on challenging problems to advance human health

and contribute towards development of techniques for the advancement of science.

Research/Work Experience

Doctoral Research- Department of Chemistry & Biochemistry, Kent State University, Kent, OH

44242, USA [Aug 2011- Present]

o Characterization of DNA secondary structures in the telomere overhang

The Structural information obtained would help in designing new class of drugs with

potential oncological targeted application – (J. Am. Chem. Soc. 2014, 136, 18062)

o Designed new small molecule drugs to target telomere G-quadruplexes based on unique

properties of telomere G-quadruplexes (target) & Small-molecule ligand binding interaction

with DNA structures in the telomere overhang

Using the specific structural information obtained from part 1, two distinct class of

molecules with potential targeted therapy capability were synthesized – (Manuscript

Under Revision)

o Effect of surface modification on human endothelial cell differentiation

Skills Acquired

o Extensive experience in molecular biology / Biomolecule Conjugation

o Single-Molecule biophysics & Microfluidic Chamber designing

o Developing & improvising binding assays to characterize Ligand-DNA interaction

o Developed programming codes to automated data analysis – Igor Pro & MATLAB

o Cell culture, Viability Assays, Immunostaining & Microscopy

o Surface modification and Characterization

o Coordinated collaborative experiments involving multiple labs of diverse expertize

o Experience in Grant/proposal Writing

List of publications & presentations

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Teaching Assistant- Department of Chemistry & Biochemistry, Kent State University, Kent, OH

44242, USA [Aug 2011- Present]

o Analytical Chemistry I & II – Focus on Analytical instrumentation & Scientific Writing

o HPLC, GC, IC, UV-Vis, fluorescence, FTIR & AAS spectroscopy & Potentiometry

o General Chemistry I & II – Fundamental Principles & Experiments

o Measurements & errors, Chemical Hygiene, pH, Stoichiometry, Titrations,

Calorimetry, Qualitative analysis, Metal complex synthesis, Redox reactions &

Electrochemistry.

Master’s Research- School of Chemical Sciences, University of Madras, Chennai-600025, India

[Sept 2009- May 2010]

Metal Ion Binding On an 8-Hydroxyquinoline Immobilized GNP/MWCNT Graphite Electrode

o Electrode modification

o Cyclic Voltammetry

o Nanoparticle synthesis and characterization

o Amperometric Quantification of metal ions

Summer Internship - Orchid Chemicals & Pharmaceuticals LTD, Chennai, India [May 2009 - June

2009]

o Hands on experience in Waters and Agilent HPLC systems

Education

*Ph.D. Candidate in Analytical Chemistry, Department of Chemistry and Biochemistry, Kent State

University, OH, USA. [2011-Present]

Current GPA: 3.8 * Graduation Date: May 13, 2017

M.Sc., Analytical Chemistry, School of Chemical Sciences, University of Madras, Chennai, India.

[2008-2010]

GPA: 4.77 / 6, Overall Grade A+

B.Sc., Chemistry, Baselius College, Mahatma Gandhi University, India. [2005-2008]

Overall Grade: First Class with Distinction (86%)

Skills

Experience in collaborative scientific experiment designing, involving multiple labs of diverse

expertise.

DNA construct synthesis for single-molecule analysis – Using molecular biology methods –

o DNA Purification protocols

o Ligation, digestion, amplification (PCR, qPCR), enzymatic site directed modification

o Molecular cloning & Bacterial culture

o Gel-electrophoresis methods (PAGE, Agarose) etc

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o Biomolecule Conjugation/ Immobilization.

o DNA footprinting assay

Single-molecule methods especially force based analysis – For the characterization of DNA

Secondary Structures

Bioanalytical analysis including

o ELISA

o Binding assays involving antibody-antigen binding interaction & Drug-DNA Binding assays

Data analysis using graphing software like Igor Pro and Origin. Can write programming codes in

Igor Pro/Matlab to automate data analysis.

Well versed in common analytical instrumentation like UV-Vis spectroscopy, fluorescence, CD,

FTIR, HPLC, IC and GC - routinely used during my doctoral research and have taught these

instrumentations in undergraduate laboratory course which was a part of my teaching

assistantship.

Experience in designing microfluidic chambers, especially for single-molecule analysis.

Surface modification of glass sides and electrode materials like carbon electrode and their

characterization with contact angle and electrochemical measurement.

Cell and bacterial culture - routinely done during my doctoral research

o Bacterial Culture for plasmid amplification

o Cell Culture on modified surfaces to study the effect of surface modification on

angiogenesis.

Can organize data and well versed in documentation using MS office applications like word, excel.

Diagram/picture drawing and organizing software like CorelDRAW, and Adobe Photoshop.

Skilled in protocol & report preparation.

Traits Quick learner/Self Starter

Attention to details

Can design new hypothesis from past knowledge and can design experiment with relevant

controls to test it with minimal supervision.

Team player & Good educator

Technology enthusiast

Research Publications & Conference Presentations

A. In refereed Journals

1. Interaction of G-quadruplexes in the full-length 3′ human telomeric overhang.

Abraham Punnoose, J.; Cui, Y.; Koirala, D.; Yangyuoru, P. M.; Ghimire, C.; Shrestha, P.; Mao, H.

J. Am. Chem. Soc. 2014, 136, 18062.

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2. Yoctoliter Thermometry for Single‐Molecule Investigations: A Generic Bead‐on‐a‐Tip

Temperature‐Control Module.

Koirala, D.; Abraham Punnoose, J.; Shrestha, P.; Mao, H. Angew Chem Int Ed Engl. 2014, 53(13),

3470.

3. Divalent cations and molecular crowding buffers stabilize G-triplex at physiologically relevant

temperatures.

Jiang, HX.; Cui, Y.; Zhao, T.; Fu, HW.; Koirala, D.; Abraham Punnoose J, Kong, DM.; Mao, H. Sci Rep.

2015, 5, 9255.

B. Manuscripts under review

1. De-Aggregation aided binding ligands specific for G-quadruplexes in telomeric overhang.

Abraham Punnoose, J.; Ma, Y.; Li, Y.; Sakuma, M.; Nagasawa, K.; Mao, H.

(Under review)

C. Manuscripts under preparation

1. Utilization of negative cooperativity of GQ formation in the full length telomere overhang in

designing DNA-Ligand conjugate ligand specific for telomere G-quadruplex.

D. Conference Presentations

1. DNA Secondary Structures in the Full-length 3’ Telomeric Overhang i.Gordon Research Conferences - Nucleosides, Nucleotides & Oligonucleotides-2015

ii.Cleveland State Interdisciplinary Research Conferenece-2015

iii.Microscopic Society of Northeast Ohio Conference-2015

2. Structural Aspects and Effect of Ligand on Full-length Telomeric Overhang

i. Zing 5th Nucleic Acid Conference-2016

Awards & Accomplishments

• Bush Prize (Honorable Mention) for Graduate teaching from Department of Chemistry & Biochemistry, Kent State University-2016

• Travel Award from Graduate Student Senate, Kent State University-2015 & 2016. • Richard Schroer Travel Award from Kent State Foundation, Kent State University-2015. • Best poster award for the presentation at Kent State Graduate Students Symposium-2015. • 2nd place for the best poster award for the presentation at the Poster competition conducted by

Kent-SAACS-2015 • 2nd place for the best poster presented at UGC sponsored National Seminar “Chemistry-Current

Focus” held at Madras Christian College, Chennai, India-2010 • NET 2011 (National Eligibility Test for college teaching conducted by University Grants

Commission and Council for Scientific and Industrial Research, India): Qualified-All India Rank: 89

• GATE 2011 (Graduate Aptitude test in Engineering conducted by IIT-India for the Ph.D. program):

Qualified- Percentile: 91.4

• Secured first rank in B.Sc., (College Level)

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• Awarded Kochett Ambattuthara Memorial Award established for the Best Out Going Student

(2008) from Department of Chemistry, Baselius college, Kottayam, India.

• Secured 3rd Place in Prof. P.J Joseph Memorial All Kerala (State Level Competition) Inter

Collegiate Science Quiz (2005), Kerala, India