Research Report 2010 - University of Kentucky

Research Report 2010

University of KentuckyMaxwell H. Gluck Equine Research Center

Improving the Health and Well-being of the Horse

Research Report 2010Improving the Health and Well-being of the Horse

University of KentuckyMaxwell H. Gluck Equine Research Center

Department of Veterinary Science108 Gluck Equine Research Center

Lexington, KY 40546-0099

Phone: (859) 257-4757Fax: (859) 257-8542

Website: http://www.ca.uky.edu/gluck

The University of Kentucky is committed to a policy of providing employment opportunities to all qualified people regardless of economic or social status and will not discriminate on the basis of race, color, ethnic origin, national origin, creed, religion, political belief, sex, sexual orientation, marital status, age, veteran status, or physical or mental disability.

Published in 2011 by the Gluck Equine Research Foundation, University of Kentucky

Dean, College of Agriculture: M. Scott Smith, PhD

Associate Dean for Research and Director, Kentucky Agricultural Experiment Station: Nancy M. Cox, PhD

Gluck Equine Research Center Director and Chair of the Department of Veterinary Science: Mats H.T. Troedsson, DVM, PhD, Dipl. ACT

Gluck Equine Research Foundation Executive Director and Director of Development and Industry Relations: Edward L. Squires, PhD, Hon. Dipl. ACT

Design and layout: Jenny BlandfordPrinted by: Thoroughbred Printing

Cover photo: Matt Barton

UK Gluck Equine Research Foundation 2010 Board of Directors

OFFICERS

Dr. Walter Zent, ChairMr. Tom Goncharoff, Vice Chair

Dr. Lee T. Todd Jr., PresidentDr. Kumble R. Subbaswamy, Vice President

Dr. M. Scott Smith, SecretaryMrs. Angela S. Martin, Treasurer

MEMBERS

Mr. Bruce Addington Mr. Alan Balch Mr. Stacy Bearse Mrs. Jane Beshear Mr. Bill Casner Mr. Case Clay Dr. Nancy Cox Mr. Tom Goncharoff Mrs. Ginny Grulke Mrs. Lisa Lourie Dr. Jamie MacLeod Dr. Everett McCorvey

Mr. Nick Nicholson Mrs. Debby Oxley Dr. Stephen Reed Mr. Dan Rosenberg Dr. Robert Stout Mr. David Switzer Mr. Oliver Tait Dr. Mats Troedsson Mr. F.E. “Butch” Wise Dr. Naoya Yoshida Mr. Chris Young Mr. Art Zubrod

A Letter from the Director 5

About the Gluck Equine Research Center 6-7

Research and Accomplishments Genetics and Genomics 10-11 Infectious Diseases and Immunology 12-19

Musculoskeletal Sciences 20Parasitology 21-22Pharmacology/Toxicology 23Reproductive Health 24-27

Gluck Equine Research Center Grants 30-32

Gluck Equine Research Center Awards 32

Scientific Publications

Books/Chapters in Books 34 Refereed Journal Articles 34-37

Non-Refereed Journal Articles 38-40 Seminars and Papers Presented 41-45

Gluck Equine Research Center Donors 47-48

Table of Contents

Research Report 2010 •

The downturn in the global economy has affected the equine industry, resulting in challenging times for horse owners, breeders, equine veteri-narians and everybody else in the equine community. The Gluck Equine Research Center is not an exception as we have adjusted our operation to manage budget cuts and devalued endowments without affecting our commitment to improving the health and well-being of horses through research. We have focused our efforts to minimize the effect of a tight bud-get on the research productivity from scientists at the Gluck Center and to further position the Gluck Center as a premier equine research center even during this difficult time.

Thanks to our committed faculty and staff, the Gluck Center had one of its more productive years ever. A few of the many accomplishments of scientists at the Gluck Center in 2010 include: identification of a potential stem cell for articular cartilage within joint tissue; development of new diagnostic tests for equine protozoal myeloencephalitis (EPM), equine in-

fluenza, equine infectious anemia, Streptococcus equi and Leptospira interrogans; the identification of a de-terminant for susceptibility for equine arteritis virus; studies on the immunology and efficacy of treatment of Lawsonia intracellularis and Rhodococcus infections in foals; improved diagnostics and treatment of mares with placentitis; and the epidemiology and disease process by which Nocardioform bacteria causes abortion.

The University of Kentucky Animal Genetic Testing and Research Laboratory (AGTRL), which is in the Gluck Center building, has gone through a couple of years of restructuring and is now operating at a much leaner and efficient level. The laboratory received the highest rating possible on the Horse Comparison Test by the International Society for Animal Genetics in 2010. This rating reflects excellent work of highly moti-vated and skilled staff. Without their hard work and loyalty to this unique service laboratory, we would not have been able to continue this important service to the horse industry.

Recognizing the emerging problem that is anticipated regarding parasite resistance to current dewormers, we have recently recruited Dr. Martin Nielsen from the University of Copenhagen in Denmark to join our exceptional group of parasitologists at the Gluck Center. Dr. Nielsen will join us later this summer.

Faculty and staff at the Gluck Center share your passion for horses and we are committed to improving the health and well-being of horses around the world. In doing so, we really need your help and support. Any contribution, small or large, during these financially challenging times will make a great difference.

Thank you for your support!

Dr. Mats Troedsson, DVM, PhD, Dipl. ACTGluck Equine Research Center Director

and Department of Veterinary Science [email protected]

Dr. Mats Troedsson

Challenging financial times, but no rest for researchers at the Gluck Equine Research Center...

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HistoryThe Maxwell H. Gluck Equine Research Center is

the only scientific institute in the United States with nearly all faculty conducting full-time research in equine health and diseases.

Construction began on the 81,000 square foot facil-ity in 1986 and was completed in 1987. The center is named after the late Maxwell H. Gluck, owner of Elmendorf Farm in Lexington. Maxwell Gluck and his wife, Muriel, generously donated $3 million to the University of Kentucky in 1983 for construction of the research facility on the condition the gift be matched by $3 million from the state and $3 million from members of the horse industry.

Research The mission of the Gluck Center is scientific

discovery, education and dissemination of knowl-edge for the benefit of the health and well-being of horses.

Today, the Gluck Center faculty conduct equine re-search in six targeted areas: genetics and genomics, infectious diseases and immunology, musculoskel-etal science, parasitology, pharmacology/toxicology and reproductive health.

Major research accomplishments of researchers at the Gluck Center has had an international impact on equine research. Some of the major re-search accomplishments include:

• World Organisation for Animal Health (O.I.E.)-designated world reference laboratory for equine rhinopneumonitis, equine influenza and equine viral arteritis

• Developed six major vaccines to protect against strangles, equine influenza, equine rhinopneu-monitis, equine viral arteritis, the shaker foal syndrome (toxoinfectious botulism) and vali-dated field safety and efficacy of equine rotavirus vaccine

• Developed diagnostic serological tests for con-tagious equine metritis (CEM), Tyzzer’s disease, equine protozoal myeloencephalitis (EPM), equine herpesvirus myeloencephalopathy, stran-gles and equine viral arteritis

• Developed enzyme-linked immunosorbent assay (ELISA) test for drug detection

• Demonstrated the usefulness of artificial lights and progesterone/estradiol treatments for hasten-ing the onset of the breeding season

• Determined the genetic basis for and developed tests for inheritance of certain color coat traits

• Provided leadership in the sequencing of the complete genome of the horse and structural characterization of horse genes

• Performed the definitive experiments that identi-fied the cause of Mare Reproductive Loss Syn-drome

The Maxwell H. Gluck Equine Research Center on the University of Kentucky campus

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knowledge of equine science through their contri-butions to basic or applied research.

The Equine Research Hall of Fame provides a lasting tribute to the most renowned equine re-searchers in a variety of disciplines and serves as an international forum for honoring outstanding achievements in equine research.Gluck Equine Research FoundationThe Gluck Equine Research Foundation was

formed as a non-profit organization to provide the exchange of information between the Gluck Center and the horse industry and to secure research funds.

Since the Foundation’s inception, it has been highly supportive in raising funds for equine re-search, endowed faculty positions, and facilities. Funding for graduate student support has allowed Gluck Center faculty to educate the next generation of scientists.

Industry OutreachProviding research information to the equine com-

munity is an important facet of the Gluck Center. Information is shared through the Gluck Center’s website, www.ca.uky.edu/gluck, and three newslet-ters:

• Bluegrass Equine Digest (monthly)• Lloyd’s Equine Disease Quarterly• Research & Service Report (biennially)Department of Veterinary ScienceThe Gluck Center is part of the Department of

Veterinary Science along with the Animal Genetics Testing and Research Laboratory (AGTRL) and the Veterinary Diagnostic Laboratory (VDL).

The mission of the Department of Veterinary Sci-ence is to assure the health and viability of animal agriculture through teaching, discovery, research and service.

Faculty in the Department of Veterinary Science frequently collaborate on research projects with faculty in UK’s College of Agriculture and College of Medicine, with veterinarians in central Kentucky and scientists at other institutions.

The Gluck Center is also part of the UK Equine Initiative, an overarching concept for all equine activities in the College of Agriculture. Created in 2005 as a front door to equine programs at UK, the Equine Initiative’s mission is to discover, share and apply new knowledge that will enhance the health, performance and management of horses commensu-rate with the signature status of Kentucky’s equine industry.

Equine Research Hall of Fame The Equine Research Hall of Fame, established by

the University of Kentucky Equine Research Foun-dation (now the UK Gluck Equine Research Foun-dation), honors those distinguished researchers who have dedicated their careers to equine science. The Hall of Fame is at the Gluck Center.

On Dec. 1, 1990, 12 scientists became the first inductees into this prestigious hall. Inductees are selected for the honor by an international scien-tific committee that evaluates the achievements and contributions of eminent researchers who were nominated by their peers and colleagues. The induct-ees, active, retired or deceased, are individuals from throughout the world who have expanded the body of

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The late Maxwell and Muriel Gluck (top) do-nated $3 million to the University of Kentucky for construction of the research facility on the condition the gift be matched by the state and members of the horse industry. (Below) James Bassett III, former president of Keeneland, Muriel Gluck and former UK President Otis A. Singletary broke ground for the center in 1985.

Twenty-two faculty at the Gluck Equine Research Center are assisted by students,

post docs and visiting scientists in conducting research in the areas of:

Genetics and Genomics

Infectious Diseases and Immunology

Musculoskeletal Science

Parasitology

Pharmacology/Toxicology

Reproductive Health

Some of the world’s top scientists are drawn to the Gluck Center to provide solutions to equine health

problems. Gluck Center faculty also respond to some of the equine industries toughest problems.


RESEARCH SNAPSHOT...•Contracted foal syndrome•Parrot mouth•Cytogenetics and infertility•Swayback

•Dwarfism •Coat color genetics •Junctional epidermolysis bullosa (JEB)

Faculty, Students & Research Assistants...Ernie Bailey, Professor DebbieCook,PhDCandidate JohnEberth,MSCandidate

Kathryn Graves, Assistant Professor

Teri Lear, Associate Professor RoseMcGee,MSCandidate JudyLundquist,ResearchTechnician

James MacLeod, Professor (Seepage20)

Ernie Bailey, PhDProfessor

Education: PhD – University of California-Davis (Genetics), 1980MS – University of California-Davis (Comparative Pathology), 1975BS – University of California-Davis (Genetics), 1973 Interest: Immunogenetics and genomics—We are interested in the genetic influences on the in-nate and adaptive immune systems which protect the horse from infectious diseases. Development of the genetic map for horses and investigation of genes involved in the health of the horse such as contracted tendons, extreme lordosis and dwarfism. Color

coat variation is also being investigated. Projects: •Genomics and gene mapping in horses.•Investigation of the hereditary aspects of EIPH, swayback, dwarfism, cataracts and equine arteritis virus susceptibility.

Graduate students focus:Debbie Cook – swaybackJohn Eberth – dwarfism

A “G gnome” stands in front of Twilight, a Thoroughbred mare at Cornell University, who was the first horse genetically sequenced in 2007. (Photo: Ernie Bailey)

Kathryn Graves, PhDAssistant Professor

Director, Animal Genetics Testing andResearch Laboratory

Education: PhD – Cornell University, 1985BS – Cook College, Rutgers University, 1980

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Horse metaphase chromosomes (the blue sticks, nuclei are round) from a male horse. Green and red spots are horse genes mapping to the X chromosome. Note: Males have one X and one Y chromosome, thus spots only show up on one X and not two as you would see in females with two X chromosomes. (Photo: Teri Lear)

Interest: Overseeing a high quality Animal Genetics Testing and Research Laboratory and providing genotyping services to 50 equine registries. In addition, the lab offers specific tests for color genes and heritable disease mutations. Projects: •Develop new DNA-based color tests.•Candidate gene sequencing to identify causative mutations for heritable diseases.

Teri Lear, PhDAssociate Professor

Education: PhD – University of Kentucky (Genetics), 1997MS – University of Louisville (Cytogenetics/Zoology), 1986BA – Indiana University Southeast (Zoology/Field Biology), 1975

Interest: Providing clinical cytogenetics services to the horse industry. Identi-fying genes causing inherited diseases such as contracted foal syn-drome, lordosis in Saddlebreds, dwarfism, parrot mouth, etc. I also

provide clinical cytogenetic services to veterinarians in the United States and abroad. This has resulted in the detection of several chromosomal abnormalities.

Clinical Cytogenetics Service: In keeping with the service and outreach mission of the University, my lab offers clinical cytogenetics services to equine veterinarians. Between 1.5% and 3% of the general horse popu-lation carries a chromosome abnormality including abnormalities of sex chromosomes, trisomy, and chromo-some translocations. Chromosome abnormalities can profoundly affect fertility and survival. Cytogenetic

techniques used in my lab can identify chromosome abnormalities causing infertility, ambiguous sex, and congenital abnormalities. We have identified chromo-some abnormalities affecting mare fertility, causing severe congenital abnormalities in foals, and causing sexual ambiguity (Lear et al. 1999; Lear and Layton 2002; Lear et al. 2008; Lear and Bailey 2008; Villago-mez et al. 2010).

Projects: •Equine chromosome disorders causing repeated early embryonic loss and congenital abnormalities.•Identifying the cause of Contracted Foal Syndrome. •Causes of XY sex-reversal syndrome in horses.•Behavioral genomics of the White-Throated Sparrow.•Genomic gains and losses that cause congenital abnormalities in foals.

Graduate student focus:Rose McGee – Disorders of sexual development in horses

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RESEARCH SNAPSHOT...•Equine rhinopneumonitis•Equine influenza•Equine viral arteritis •Equine infectious anemia•Equine rotaviral enteritis •Strangles and other equine streptococcal diseases•Equine leptospirosis•Equine clostridial enteritis•Lawsonia intracellularis enteropathy•Rhodococcal pneumonia•Diagnostic test development

•Vaccine development•Disease surveillance and reporting •Biosecurity/Disaster preparedness•The development of immunity in the foal•The immune response in aged horses•Identification of vaccine-induced protective immune responses•Characterization of inflammatory responses in the horse•Cytokine regulation of immunity in the horse

Faculty, Students & Research Assistants...Sergey Artiushin, Assistant Professor

Udeni Balasuriya, Associate Professor JulianaCampos,MSCandidate PamelaHenney,ResearchSpecialist YanqiuLi,PostdoctoralScholar ZhengchunLu,PhDCandidate KristinPfahl,MSCandidate KathrynSmith,PhDCandidate JianqiangZhang,PostdoctoralScholar Undergraduates: BeverlyBalasuriya BoraNam GongSeoul JenaWhite

Thomas Chambers, Associate Professor StephanieReedy,ResearchSpecialist SanjaySarkar,PhDCandidate AshishTiwari,PhDCandidate LiangZhang,PhDCandidate

Frank Cook, Assistant Professor DebbieEven,PhDCandidate

Roberta Dwyer, Professor

David Horohov, Professor AmandaAdams,PostdoctoralScholar AlexBetancourt,ResearchTechnician ChongLiu,PhDCandidate AllenPage,PhDCandidate LingshuangSun,PhDCandidate Undergraduates: DanielMichler

CraigStewart EricOberst SeanSiaens

Charles Issel, Professor SheilaJ.Cook,ResearchScientist

John Timoney, Professor RafaelaDeNegri,PhDCandidate MichaelFettinger,ResearchTechnician Marie-LucieStyza,ExchangeScholarfrom France SridharVelineni,PostdoctoralScholar

Peter Timoney, Professor YunYoungGo,PhDCandidate JessicaHennig,MSCandidate BarryMeade,PhDCandidate KathleenShuck,ResearchAnalystPrincipal

Visiting scientists in 2010: JulianaFulgencio,VisitingScholar(Brazil)KatherineHughes,DVMstudent(USA)MuhammadIjaz,DVM,M.Phil,PhD(Pakistan)JuliaKrause,PhDstudent(USA)Soo-JeongKye,DVM,MVSc(Korea)NettieLamprecht,PhDstudent(USA)MelanieLean,MSstudent(UnitedKingdom)FabienMiszczak,PhDstudent(France)KalimuthusamyNatarajaseenivasan,PhD,BSc,MSc(India)DanShen,veterinarian,(China)LisaTadros,PhDstudent(USA)IvanFranciscoVazquez,MSstudent(Spain)


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Sergey Artiushin, PhDAssistant Professor

Education: PhD – Moscow State University (Microbiology), 1981MS – Moscow Veterinary Academy (Biophysics), 1973 Interest: Research interests are focused on molecular studies of Streptococcus equi, Streptococcus zooepidemicus, and Leptospira interrogans. S. equi causes stran-gles and S. zooepidemicus is responsible for endometritis in mares. Leptospiro-sis can cause abortion and stillbirth as well as recurrent uveitis (a major cause of equine blindness). Projects:

•Development of rapid diagnostic assays for identification of bacterial pathogens.•Study of surface and secreted proteins of Streptococcus as virulence factors and potential protective antigens.•Analysis of genetic variations in Streptococcus.•Identification of virulence factors of S. zooepidemicus responsible for developing acute infection in dogs and horses.

Udeni Balasuriya, PhD, MS, BVScAssociate Professor

Education: PhD – University of California-Davis (Comparative Pathology with special emphasis in molecular virology), 1996MS – University of California-Davis (Comparative Pathology with special emphasis in diagnostic pathology), 1991BVSc – Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka, 1985

Interest: The major research focus of my laboratory is to characterize the molecular epidemiology and pathogenesis of equine arteritis virus (EAV) and equine herpesvirus-1 (EHV-1) infections of horses and develop improved recombinant vaccines to prevent infection of horses with these viruses, as well as to develop

improved tests to diagnose the infection. In addition, I have established national and international collabora-tions to facilitate exchange of scientists, reagents and information focused on EAV, EHV-1 and other equine viral diseases. My laboratory also provides a dynamic, first-rate research training environment to develop the next generation of research scientists.

Projects:•Molecular characterization of equine arteritis virus (EAV) and equine herpesvirus-1 (EHV-1).•Definitively characterize the molecular epidemiology and pathogenesis of EAV and EHV-1 infections of horses and develop improved recombinant vaccines to prevent infection of horses with these viruses as well as improved tests to diagnose the infection. •Molecular mechanisms of viral pathogenesis and characterization of host immune response to EAV infection. •Host-virus interactions (e.g. analysis of individual viral genes/gene products and their interaction with host).•Development of new diagnostic and vaccine technologies, define the epidemiology and pathogenesis of other important viral diseases of the horse, and the recognition of novel and emerging viral diseases of the horse.•Establish national and international collaborations to facilitate exchange of scientists, students, reagents and


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information, all focused on EAV, EHV-1 and other important viral diseases of the horse.

Services:•Provide assistance with molecular diagnostics (RT-PCR and real-time RT-PCR).•Testing of clinical specimens submitted to the OIE designated reference laboratory for equine viral arteritis (EVA) at the Gluck Equine Research Center.•Testing of clinical specimens submitted to the OIE designated reference laboratory for equine influenza at the Gluck Equine Research Center.•Provide molecular diagnostic reagents to diagnostic laboratories.•Provide advice on equine viral arteritis to veterinarians and equine industry associates over the phone.

Postdoctoral/Graduate students focus:Juliana Campos – Genetic susceptability of stallions to EAVYanqiu Li – Molecular characterization of neurovirulent EHV-1Zhengchun Lu – EAV attachment and entry and molecular diagnosticsKristin Pfahl – Development and validation of improved serological assays for EVAKathryn Smith – Virulence determinants of EHV-1Jianqiang Zhang – Molecular characterization of EAV

Thomas Chambers, PhDAssociate Professor

Education: PhD – University of Notre Dame (Microbiology), 1982BS – University of Notre Dame (Pre-Professional Studies), 1975

Interest:Equine influenza is the leading cause of respiratory disease in Kentucky and the world. My major interest is to study the innate immune responses to the influ-enza virus and herpes virus. I am also interested in the development of vaccines for influenza and herpes virus. I am involved in infectious disease control and surveillance both nationally and internationally.

Projects: •Infectious diseases and immunology.•Testing in equines of a second-generation modified-live virus equine influenza vaccine.•Testing/validation of second-generation rapid diagnostic tests for equine influenza.•Testing in equines of novel DNA-based vaccines for equine influenza.•Testing in equines of new vaccination protocols for equine influenza using an existing commercial vaccine.•Collaborator on development of the most up-to-date phylogenic description of the strains of equine influenza virus in circulation since 1990.•Collaborator on development of a new real-time RT-PCR assay for equine influenza.•OIE International Reference Laboratory for equine influenza.

Graduate students focus:Sanjay Sarkar – innate immunity to equine viral respiratory diseasesAshish Tiwari – innate immunity to equine viral respiratory diseasesLiang Zhang – comparative pathogenesis of equine- and equine-2 influenza viruses


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R. Frank Cook, PhDAssistant Professor

Education: PhD – University of Warwick (Virology), 1980BSc – University of Sussex (Biochemistry), 1976 Interest: My research focuses on Equine Infectious Anemia Virus (EIAV), a virus closely related to HIV in humans. My most recent interests are in the field of vaccine design.

Projects: •Design of vaccines against all lentiviruses including HIV-1.•Provide purified antigens for inclusion in commercial USDA-approved test kits.•Strategies to enhance efficacy of DNA vaccination in the horse (i.e., the use of cytokines to enhance the ef-ficacy of vaccines).•Molecular epidemiology of equine herpesviruses.•Genetic basis of differing susceptibility to disease and immune responses to vaccinations.

Graduate student focus:Debbie Even – Manipulating immune responses to DNA vaccines in the horse

Roberta Dwyer, DVM, MS, DACVPMProfessor

Education: DVM – Iowa State University, 1985MS – University of Kentucky, 1990Diplomate, American College of Veterinary Preventive Medicine, 1993Board Certified in Epidemiology, ACVPM, 2003 Interests: Equine preventive medicine and infectious diseases, disease outbreak investi-gation and epidemiology, biosecurity, disaster preparedness and response, risk reduction to agroterrorism and pre-veterinary advising and undergraduate teaching.

Projects: •Consultations for veterinarians, farm managers and horse owners.•Biosecurity plan development for veterinary and farm facilities.•AAEP on-call media veterinarian for infectious disease issues.•Instructor for a national extension program, “Strengthening Community Agrosecurity Plans.”•Co-editor of Lloyd’s Equine Disease Quarterly.•Planning section chief for a national Incident Management Type II team (disaster response team).


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David W. Horohov, PhD William Robert Mills Chair & Professor

Education:Professor of Veterinary Immunology, Louisiana State University, 1988-2003Fellowship – FDA, Bethesda, MD, 1986-1988PhD – University of Tennessee, 1985 MS – Purdue University, 1981BS – Pennsylvania State University, 1978

Interest: My group continues to investigate the immune responses of horses to various infectious diseases. We are also interested in

infections that occur later in the life of the foal, such as Lawsonia intracellularis, and age-related changes in the immune function in older horses. We also collaborate with other groups to study the characterization of cell-mediated immune responses in EIA-infected and vaccinated horses, vaccination of foals against equine influenza virus, further investigations into parasite immunology and the characterization of inflammatory responses to exercise.

Projects: •Underlying immunological basis for the susceptibility of foals to infection with Rhodococcus equi, a cause of pneumonia in foals less than 3 months old. •Investigating the underlying molecular mechanism for low levels of interferon-gamma, a cytokine that plays a central role in resistance to R. equi and other pathogenic organisms. (Linshuang Sun)•Infections that occur later in the life of the foal, such as Lawsonia intracellularis, the causative agent for equine proliferative enteropathy (EPE). (Allen Page and Amanda Adams)•Role of nutritional supplements in restoring immune function in aged horses. (Amanda Adams) •Older horse immune function. Collaborating with Kristine Urshel, UK Department of Animal Science, who is interested in the effect of aging on protein metabolism in horses, and Nicholas Frank, University of Tennessee, who is interested in metabolic syndrome in horses. Frank’s group is also interested in obesity-related inflam-matory changes and their role in the induction of metabolic disease in the horse. •Interactions between adipocytes and the immune system. (Amanda Adams) •Cell-mediated immune responses in EIA-infected and vaccinated horses. The goal will be to identify immu-nological responses that may be important in controlling viral replication and disease. (Chong Liu, in collabo-ration with Charles Issel at the Gluck Center and Ron Montelaro at Pittsburgh) •Other collaboration projects include: vaccination of foals against equine influenza virus with Thomas Cham-bers at the Gluck Center; parasite immunology with Gene Lyons at the Gluck Center; and characterization of inflammatory responses to exercise with Ken McKeever, at Rutgers University. Our goal is to identify immu-nological markers for exercise-induced inflammation in the horse and to determine if these correlate with the risk of injury in the performance horse.

Postdoctoral/Graduate students focus:Amanda Adams – Aged horses and obesityChong Liu – Cell mediated immunity to EIAVAllen Page – Lawsonia intracellularis infection and immunityLingshuang Sun – Regulation of interferon in foals


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Charles Issel, DVM, PhDWright-Markey Chair in Equine Infectious Diseases & Professor

Education: Diplomate – American College of Veterinary Microbiologists, 1976PhD – University of Wisconsin (Veterinary Science), 1973MS – University of Wisconsin (Veterinary Science), 1971 DVM – University of California–Davis, 1969AB – University of California–Berkeley (Zoology), 1965

Interest: Our research, continuous since 1974, involves equine infectious anemia from A to Z. We are working with the national and international veterinary community to develop and implement sensitive, specific and practical diagnostic tests for EIA to complement the “Coggins” test in effective control programs. Our work

indicates that we should adopt a three-tiered testing approach which would more accurately detect positive horses with difficult to read AGID test reactions (see center photo below with three such positive samples).

At the same time we are studying the intricacies of the EIA virus (EIAV), a lentivirus, in an attempt to define the genetic and antigenic variations in this highly mutable agent and how it impacts protective immunity, i.e., vaccine design and efficacy. This work is valuable in its own right as well as being of comparative value as a model for AIDS.

Our projects with the USDA have resulted in a DVD package that includes our video from 1996 and a new video from 2010, “Equine Infectious Anemia Testing – Refining our approach.” Both videos are available online at http://www.aphis.usda.gov/animal_health/animal_diseases/eia/. To obtain the DVD package, visit http://www.aphis.usda.gov/vs/nahss/equine/eia/index.htm.

Projects: •Improve diagnosis and control of EIA.•Develop effective vaccines against EIA.•Provide high quality reagents for use in testing for diseases.•For more information on EIA please see our website at: http://dept.ca.uky.edu/eia/.

Horseflies are vectors of EIA (Photo by Alex Wild)

Coggins test plate Humans are still major vectors of EIA

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John Timoney, MVB, MRCVS, MS, PhDProfessor

Education: PhD – National University of Ireland, 1969MS – University of Wisconsin, 1967MVB, MRCVS – University College, Dublin, 1965BSc – University College, Dublin (Biology), 1961

Interest: Focus is on equine infectious disease caused by streptococci, leptospira, salmo-nella and clostridia. Ultimate goals include development of improved vaccines, diagnostics and design of strategies effective in management of outbreaks and detection of infected horses.

Projects: •Use of the binding sites of Toxins A and B of Clostridium difficile as immunogens to generate toxin neutral-izing antibody in pregnant mares. •Identification and regulation of virulence factors of Streptococcus zooepidemicus from acute equine and ca-nine pneumonias.•Wildlife source(s) of the specific genotype of Leptospira interrogans serovar Pomona responsible for equine abortions in Kentucky.•Development of novel modalities for intranasal vaccination of horses against strangles.•Rapid diagnostic assays for Streptococcus equi and Leptospira interrogans in equine clinical specimens. •Development of protocols for prepartum immunization of mares for prevention of neonatal enterocolitis caused by clostridia and salmonella.•Role of bacteriophage in virulence and evolution of Streptococcus equi.•Identification of proteins of Leptospira interrogans induced following ocular and placental infection of the horse.•Interaction of Streptococcus equi with the equine tonsillar complex.

Postdoctoral/Graduate students focus:Rafaela De Negri – Streptokinases of Streptococcus equi and zooepidemicusMarie-Lucie Styza – Detection of serum antibodies specific for Clostridium difficile Sridhar Velineni – Regulation of virulence in Streptococcus zooepidemicus

A scanning electron micrograph (left) shows adherence of Streptococcus equi, the cause of equine strangles, to stratified squamous epithelium of the equine lingual tonsil. The photo (right) shows S equi within the epithelium of the nasopharyngeal tonsil of a horse three hours after infection. (Photos: John Timoney)


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Peter Timoney, MVB, PhD, FRCVSFrederick Van Lennep Chair in Equine Veterinary Science,

OIE Designated Specialist on Equine Viral Arteritis & Equine Rhinopneumonitis

Education: FRCVS – Royal College of Veterinary Surgeons, 1978PhD – University of Dublin, 1974MS – University of Illinois, 1966MVB (Hons) – National University of Ireland, 1964 Interests: Among the diseases of major interest are equine viral arteritis (EVA), conta-gious equine metritis (CEM) and equine rhinopneumonitis. Also, development of strategies for reducing the risk of global spread of infectious diseases through

international trade.

Projects: •Studies on the mechanism of establishment and persistence of equine arteritis virus in the reproductive tract of the stallion.•Characterizing the site(s) of localization of equine arteritis virus in the carrier stallion.•Investigating the variation in virulence among naturally occurring strains of equine arteritis virus.•Developing a less costly, more rapid diagnostic test for EVA.•Developing a second generation marker vaccine for EVA/equine arteritis virus infection.•Developing a more rapid and reliable diagnostic test for detection of Taylorella equigenitalis.•Improving control and prevention strategies for contagious equine metritis (CEM).•Study of the epidemiology of Taylorella asinigenitalis in horses and non-horse equids.

Services:•Responsible for operation of the OIE Reference laboratory for equine viral arteritis at the Gluck Equine Re-search Center.•Provision of EVA diagnostic reagents to diagnostic laboratories, nationally and internationally.•Provide consultation for veterinarians and members of the horse industry on various equine infectious dis-eases, including EVA, equine rhinopneumonitis, contagious equine metritis and equine piroplasmosis.•Co-editor of the Lloyd’s Equine Disease Quarterly.•Provide quarterly reports to the International Collating Centre, Animal Health Trust, Newmarket, UK, on equine infectious disease occurrences in the United States.

Graduate students focus:Yun Young Go – Molecular characterization of EAV humoral antibody response of horse to non-structural virus proteins and host virus interactionsJessica Hennig – To Be Determined Barry Meade – Comparisons of the transmission dynamics of disease outbreaks attributable to neuropatho-genic and non-neuropathogenic strains of EHV-1 in closed populations


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•Osteoarthritis•Articular cartilage maturation and repair

James MacLeod, Professor StephenColeman,PhDCandidate RebekahCosden,PhDCandidate LaurenDetlefsen,MS(graduated,2010)

JenniferJanes,PhDCandidate KadieVanderman,MS(graduated,2010) WenyingZhu,PhDCandidate

James MacLeod, VMD, PhDProfessor

Education: Fellowship – University of Pennsylvania (Endocrinology & Genetics), 1992PhD – University of Pennsylvania, 1990VMD – University of Pennsylvania, 1984BS – University of Delaware, 1980

RESEARCH SNAPSHOT...

Faculty, Students & Research Assistants...

Interest: The laboratory studies biological and biomedical aspects of the musculoskeletal system, with an emphasis on the growth and maturation of articular cartilage, the development of osteoarthritis, repair of articular lesions and the effects of anti-inflammatory medications. Experiments are conducted primarily on a cellular and mo-lecular level. In addition to articular cartilage, recent projects have been initiated on tendons, cervical stenotic myelopathy (Wobbler Syndrome), and broad analyses of the equine transcriptome.

Projects: •Articular cartilage maturation.•Articular cartilage repair.•Intra-articular glucocorticoid therapy.•Tendon maturation.•Wobbler syndrome (cervical stenotic myelopathy).•Horse genomics: gene expression.

Graduate students focus:Stephen Coleman – equine transcriptomeRebekah Cosden – articular cartilage maturation and repair Jennifer Janes – pathogenesis of cervical vertebral stenotic myelopathy Wenying Zhu – osteoarthritis and intra-articular glucocorticoid therapy

Graduate degrees complete:Lauren Detlefsen (MS) – tendon maturation and response to biomechanical stressKadie Vanderman (MS) – chondrocyte cell biology

•Wobbler Syndrome•Tendons and ligaments


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yRESEARCH SNAPSHOT...•Helminths, including life cycles, prevalence and control

•Equine protozoal myeloencephalitis •Parasite resistance

Faculty, Students & Research Assistants...Daniel Howe, Associate Professor SrivenyDangoudoubiyam,Postdoctoral Scholar BreannaGaubatz,MSstudent AbleshGautam,PhDCandidate MichelleYeargan,ResearchSpecialist

Eugene Lyons, Professor

SandraCollins,SeniorLaboratory Technichian SharonTolliver,ResearchSpecialist

Visiting Scientists: UllaAndersen,DVM(Denmark) TetianaKuzmina,PhD(Ukraine) IvanFranciscoVazquez,BVM(Spain) MaríaSolAriasVázquez,PhD(Spain)

Daniel Howe, PhDAssociate Professor

Education: PhD – Purdue University (Molecular Parasitology), 1992MS – Western Illinois University (Biology/Parasitology), 1990BS – Western Illinois University (Biology), 1988 Interest: Molecular studies of protozoan parasites – The primary research goal is to obtain a better understanding of the parasite Sarcocystis neurona, the primary cause of equine protozoal myeloencephalitis (EPM). Studies are ongoing to determine the

genome sequence for S. neurona. Other interests include the development of improved serum assays for EPM diagnosis. Additionally, we are investigating approaches to develop an effective vaccine against EPM.

Projects: •Characterization of novel genes and antigens from the parasite Sarcocystis neurona.•Development of a serologic assay for diagnosis of EPM and to develop a vaccine for EPM.•Sequencing and annotation of the S. neurona genome.

Graduate students focus:Breanna Gaubatz – Genetic analysis of EPM horsesAblesh Gautam – Characterization of the SnSAG family of surface antigens in Sarcocystis neurona

Eugene Lyons, PhDProfessor

Education: PhD – Colorado State University (Parasitology), 1963MS – Kansas State University (Parasitology), 1958BS – South Dakota State University (Wildlife), 1956 Interest: Parasitology: Control and transmission of internal parasites of horses. Nearly all dewormers currently on the market were tested for efficacy on internal


Fluorescently-labeled Sarcocystis neurona parasites (green) adjacent to the infected cell’s nucleus (blue). (Photo: Dan Howe)

Bot (Gasterophilis intestinalis) insect larvae of horses. First, second and third instars found in the mouth and/or stomach (on left) and first instar in an egg attached to a horse hair (on right). (Photos: Gene Lyons)

parasites here in the Department of Veterinary Science. Studies are ongoing on resistance of small strongyles and ascarids to commercial dewormers. Other research is concentrated on profiling the passage of small stron-gyle eggs in feces (EPGs) of older horses. This is to try and determine which animals are low egg shedders, and thus don’t need deworming, and which ones are “high” egg shedders, which are needing antiparasitic treatment. Other research is on internal parasites of wildlife, especially hookworms in pinnipeds. Projects: •Control, transmission and prevalence of natural infections of internal parasites of horses.•Drug resistant nematodes in field and critical/controlled tests.•Seasonal and yearly transmission.•Molecular identification of parasite species and basis of drug resistance.


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Thomas Tobin, MVB, MSc, PhD, MRCVS, DABTProfessor of Veterinary Science and the Graduate Center for Toxicology

Education: DABT – Diplomate, American Board of Toxicology, 1980PhD – University of Toronto (Pharmacology), 1970MSc – University of Guelph (Pharmacology), 1966 MVB – University College, Dublin, 1964

Contributions: 1983: Regulatory threshold for furosemide. 1985-Present: About 100 ELISA tests for equine medications, licensed to Neogen Corp. 1997: US Patent for the first FDA approved treatment for Equine Proto-zoal Myelitis, licensed to Bayer.2003: US Copyright on unique biological mechanism of MRLS. 2006: US Patent for novel veterinary tranquilizer. 2011: Certified reference and internal standards for therapeutic medica-tion regulation.

Projects:•Ongoing research on certified reference standards and internal standards licensed to Neogen Corp. •Creation of ELISA tests for drug detection and certified reference stan-dards for therapeutic medication regulation.•Developing animal models of ocular, fetal and central nervous system parasitic disease and demonstrating the therapeutic efficacy of specific chemotherapeutics. •Develop improved assays for ergot alkaloid analysis, the group of toxins involved in fescue toxicosis.

Postdoctoral/Graduate students focus:Relationships between medication or medication residue concentrations and pharmacological effects in the contexts of resulting therapeutic responses and/or the regulatory significance of medication residues with respect to competitive events.

Rompun®, the widely used short acting equine tranquilizer is detect-ed in equine urine as 4–hydroxyx-ylazine, a Rompun® metabolite fragment. We have synthesized 4–hydroxyxylazine (above) for use as a regulatory standard. This X-ray crystallograph definitively estab-lishes the identity and structure of our reference standard. (Photo: Thomas Tobin)

RESEARCH SNAPSHOT...•Therapeutic medication regulation•Establishing world wide reference standards for therapeutic medications, dietary and environmental substances. •Developing novel ELISA-based forensic tests

•Development of specific and sensi-tive tests to detect ergot alkaloids (associated with fescue toxicity and other diseases)•Development of novel therapies for infectious and other diseases

Faculty, Students & Research Assistants...Thomas Tobin, Professor CharlieHughes,ResearchAssociate JulioGutierrez,PostdoctoralFellow

GabrielleHerrensmith,Studentassistant EmilySchwartz,Studentassistant


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Graduate students focus:Lauren Keith – Suppression of estrous in race maresJuliana Almeida (PhD, UC Davis) – Anti-Mullerian hormone in the horseLiz Scholtz (PhD, UC Davis) – Role of 5-alpha reduced progestins in equine pregnancy

Barry Ball, DVM, PhD, Dipl. ACTProfessor

Education: Dipl. ACT – 1987PhD – Cornell University, 1987DVM – University of Georgia, 1981

Projects:•Studies on anti-Müllerian hormone in the mare and stallion.•Down regulation of oxytocin receptors and luteal maintenance in mares.•Bioactivity of 5 α-dihydroprogesterone in mares.•Diagnostic methods related to placentitis and late abortion in mares.•Nonsurgical control of reproductive behavior in the stallion.


RESEARCH SNAPSHOT...•Causes, diagnosis and treatment of embryonic and fetal loss in mares •Early embryonic development •Uterine infection•Nutritional affects on reproduction•Stallion behavior•Diagnosis and treatment of fertility problems in stallions•Fescue toxicosis

Faculty, Students & Research Assistants...Barry Ball, Professor LaurenKeith,MSCandidate

Karen McDowell, Associate Professor DrewHestad,MSCandidate

Ed Squires, Professor KatherynCerny,MSCandidate SydneyHughes,MSCandidateTom Swerczek, Professor

Mats Troedsson, Professor KirstenScoggin,PhD,SeniorScientist ClaudiaKlein,PhD(graduated,2010) LyndaMiller,PhD(graduated,2010) AnaGabriellaToroMayorga,MSCandidate ElizabethWoodward,PhDCandidate

Visiting Scientists: MohammedAbabneh,DVM,PhD(Jordan) MetteChristoffersen,DVM(Denmark) MortenMøllerPetersen,DVM,PhD,Dipl. ACT(Denmark) JasmineWalters,DVM(Germany)

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Karen McDowell (left) and Elizabeth Moore, an Agricultural Biotechnology student, use a doppler ultrasound to look at blood flow in horses in an endophite-infected fescue study.

Karen McDowell, PhD, EMBAssociate Professor

Education: EMB – Certified Embryologist, American College of Em-bryology, 2010NIH Postdoctoral Fellow – Colorado State University (Physiology), 1987PhD – University of Florida (Animal Science), 1986MS – University of Tennessee (Animal Science), 1980BS – University of Tennessee (Animal Science), 1976 Interest: Determining causes of reproductive losses in mares, includ-ing maternal-embryo or maternal-fetal interactions, mare reproductive loss syndrome, and most currently, effects of endophyte-infected fescue on pregnant mares. Overall goal

is to reduce pregnancy losses and enhance pregnancy maintenance in mares.

Projects: •Vascular changes associated with consumption of endophyte-infected fescue.•In vitro assessment of ergot alkaloids, receptor agonists and receptor antagonists on equine peripheral and central arteries.

Graduate students focus:Drew Hestad – Equine fescue toxicosis

Edward Squires, PhD, Dipl. ACT (hon.)Professor, Executive Director of the Gluck Equine Research Foundation and

Director of Advancement and Industry RelationsEducation: Dipl. ACT (hon.) – 2003PhD – University of Wisconsin, 1974MS – West Virginia University, 1971BS – West Virginia University, 1969 Interest: Improving the reproductive efficiency of both mares and stallions, hormonal control of the cycle and development of reproductive techniques. Sydney Hughes (MS student) is conducting two projects: 1) investigating the viability, economic value and performance of offspring from mares that were treated for

placentitis during late gestation; and 2) the effect of age, season and status of the mare (barren, maiden, foal-ing) on dystocia (difficult births). Katheryn Cerny is studying the transmission of bacteria from stallions to mares. Projects: •Examination of farm records: performance of surviving foals out of mares treated for placentitis and inci-dence and outcomes of dystocia (Hughes)•Potential transfer of bacteria from infected stallions to mares–does this occur and then affect pregnancy rates? (Cerny)

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Tom Swerczek, DVM, PhDProfessor

Education: PhD – University of Connecticut (Comparative Pathology), 1969 MS – University of Connecticut (Nutritional Pathology), 1966 DVM – Kansas State University, 1964BS – Kansas State University, 1962 Interest: Nutritional Pathology. Factors that cause abortion in mares, includ-ing climatic and environmental changes that induce stress to pasture forages. Drought, excessive rainfall, frosts and freezes can induce nutrient imbalances.

Projects: •Evaluation of bacterial endophytes of grass-and-legume forages as emerging causes of reproductive loss in horses.•Develop diagnostic tests for tissues and blood to aid in diagnosis of fetal loss.

•Density gradient centrifugation of frozen semen (Hughes and Cerny)•Induce twin pregnancy to double eCG levels (Hughes)•Uterine rupture in mares (Toro Mayorga)•Establishment of sustained EAV infection (Campos)

Graduate students focus:Katheryn Cerny – Transmission of bacteria from the stallion to the mare and its effect on pregnancy rate and embryonic lossesSydney Hughes – Reproductive problems in late pregnancy mares


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Mats Troedsson, DVM, PhD, Dipl. ACT, Dipl. ECARProfessor, Director of the Gluck Equine Research Center, and

Chair of the Department of Veterinary Science

Education: Dipl. ECAR – 2002Dipl. ACT – 1993 PhD – University of California – Davis, 1991DVM – Royal Veterinary College (Stockholm, Sweden), 1975

Interest:Equine reproductive health and biology. 1) The interaction between spermatoza and the uterine immune system with a particular note on its role in breeding-induced endometritis. 2) The role of seminal proteins in fertility. 3) Claudia Klein, PhD, is using a genomic approach to study the interaction between the mares’ uterus and the early conceptus during the critical time of pregnancy recognition. 4) Diagnostics and treatment of high-risk pregnancies.

Projects:•Interaction between semen and the uterus in horses.•Identification of seminal plasma proteins that affect fertility.•Sperm surface proteins. •Early pregnancy recognition and losses.•Inflammation of the uterus – role of nitric oxide and inflammatory cytokines. •Causes, diagnostics and control of high-risk pregnancies.

Graduate students focus:Ana Gabriella Toro Mayorga – uterine pathology/biopsiesElizabeth Woodward – endometritis

PhD Dissertations:Claudia Klein. 2010. Early pregnancy recognition/genomicsLynda Miller. 2010. Sperm surface proteins


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Gluck Equine Research Center

Competitive Grant Funding

Non-Competitive Grant Funding

Awards

The mission of the Gluck Center is scientific discovery, education and dissemination of knowledge for the benefit of the health and

well-being of horses.

Artiushin, S.C. 2008-2010. Kentucky Science and Engineering Foundation. Rapid test for S. equi and L. interrogans in clinical specimens from horses. (Co-PI: Timoney, J.F.) $42,393.

Artiushin, S.C. 2010-2011. Grayson-Jockey Club Re-search Foundation. Toxins TcD A and B of Clostridium difficile for horse immunization. (Co-PI: Timoney, J.F.) $45,885.

Balasuriya, U.B.R. 2010-2012. Grayson-Jockey Club Research Foundation, Inc. Molecular characterization of neurovirulent EHV-1 strains. (Co-PI: Cook, R.F. and Timoney, P.J.) $95,361.

Balasuriya, U.B.R. 2010-2011. Kentucky Horse Racing Commission. Comparison of in vitro antiviral activity of herpesvirus DNA polymerase inhibitors against neuro-pathogenic and non-neuropathogenic strains of equine herpesvirus-1. (Co-PI: Cook, R.F.) $40,499.

Balasuriya, U.B.R. 2010-2011. Kentucky Equine Drug Research Council. Testing the susceptibility of neuro-pathogenic and non-neuro-pathogenic equine herpesvi-ruses-1 strains to antivirals. (Co-PI: Cook, R.F., Tobin, T., Timoney, P.J.). $39,745.

Balasuriya, U.B.R. 2008-2010. Fort Dodge Animal Health Research Agreement. Development and Charac-terization of Equine Arteritis Virus Vectored Chimeric Viruses as Vaccines against Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). (Co-PI: Tim-oney, P.J.) $211,000, total costs for two years.

Chambers, T.M. and Horohov, D.W. 2009-2010. Inter-vet/Scherring Plough. Does vaccination of very young foals with Fluavert in the presence of maternal antibod-ies protect them from infection? $35,543.

Chambers, T.M. and Horohov, D.W. 2010-2011. Fur-ther characterization of the immunological response of horses to vaccination with Fluvac Innovator. Contract. $88,802.

Cook, R.F., Horohov, D.W. and Issel, C. 2009-2014. NIH. EIAV envelope variation and vaccine efficacy. Co-

Competitive Grant FundingPIs (PI: Montelaro, R.), $1,737,500 ($3,668,813 total).

Dwyer, R.M. 2009-2010. CSREES/EDEN. Strengthening Community Agrosecurity Planning (S-CAP) Train-the-Trainer Project: Phase 2. Co-PI (PI: Husband, A. Co-PIs: Yeargan, R. and Newman M.), $101,000.

Dwyer, R.M. 2009-2011. Extension Disaster Education Network with CSREES funding. The EDEN Strengthen-ing Agrosecurity Planning Train-the-Trainer Program. Co-PI (PI: Husband, A. Co-PIs: Yeargan, R. and Newman M.), $75,294.

Horohov, D. W. 2009-2011. USDA-AFRI. The effect of age on equine dendritic cell interactions with Rhodococ-cus equi. $200,000.

Horohov, D.W. 2010-2011. Equine Drug Research Coun-cil. Seroprevalence of Lawsonia intracellularis in central Kentucky Thoroughbred weanlings. $45,841.

Horohov, D.W. and Lyons, E.L. 2010-2011. Pfizer Ani-mal Health. Characterization of the inflammatory response to anthelmintics. Contract. $38,473.

Horohov, D.W. 2010-2011. Pfizer Animal Health. Further characterization of the immunological response of horses to Metastim. Contract. $48,193.

Howe, D. 2009-2012. USDA/CSREES. Genome Se-quence for the apicomplexan Sarcocystis neurona. $500,000.

Lear, T. 2009-2011. National Institutes of General Medi-cal Sciences, National Institutes of Health. Behavioral ge-nomics of the White-Throated sparrow. Co-PI. (E. Tuttle, PI, Indiana State University). $84,000.

Lear, T. 2009-2010. Gluck Equine Research Center. Ge-netic determinants associated with contracted tendons in foals. (Co-PIs: Bailey, E. and Carter, C.) $7,500 (internal funding).

MacLeod, J.N. 2009-2011. Morris Animal Foundation. Orthopaedic pathology and genetic associations in cervi-cal stenotic myelopathy. Sponsor for Jennifer Janes, Total

Funding is important to equine research. The faculty at the Gluck Equine Research Center were successful in obtaining several competitive and non-competitive grants, some of which were multi-year grants.


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Competitive Grant Fundingdirect support: $100,000.

MacLeod, J.N. 2009-2012. National Science Foundation. Exon splice pattern characterization of the whole mRNA transcriptome. Co-PI (PI: Liu, J.). Total direct support: $1,027,384.

MacLeod, J.N. 2010-2012. Kentucky Horse Racing Commission. Safety and anti-inflammatory efficacy of glucocorticoids for intra-articular therapy in racehorses. PI. $99,969.

MacLeod, J.N. 2010-2012. Grayson-Jockey Club Foun-dation. Orthopaedic Pathology and Genetic Associations with Cervical Stenotic Myelopathy. PI. Total direct sup-port: $102,193.

McDowell, K.J., Lawrence, L., Aiken, G., Bush L. and Horohov, D. 2007-2013. USDA-ARS-FAPRU. “Vascular Changes Associated with Consumption of E+ Tall Fescue by Broodmares. $187,044.

Squires, E.L. 2009-2010. Preservation of Equine Genet-ics Fund, Colorado State University. Pregnancy rates of equine embryos biopsied for genetic testing. Co-PI (Se-idel, Jr., G.E. (PI), Colorado State University) $36,443.

Squires, E.L. 2009-2010. Preservation of Equine Genet-ics Fund, Colorado State University. Capacitating stallion sperm to enhance in vitro fertilization. Co-PI (Graham, G.E. (PI), Colorado State University) $30,000.

Squires, E.L. 2010-2011. Kentucky Equine Drug and Racing Commission. Estrus suppression in the mare. Co-PI: Troedsson, M.H.T.) $30,000.

Squires, E.L. 2010. Bioniche Animal Health. Use of eFSH to increase eCG production in mares. (Co-PI: Troedsson, M.H.T.) $8,000.

Squires, E.L. 2010. Preservation of Equine Genetics Program. Colorado State University. Fertility of mares inseminated with frozen/thawed semen centrifuged through a single layer density gradient. (Co-PI: Troeds-son, M.H.T.) $36,225.

Timoney, J. 2009-2011. USDA (pending). Identification of surface and secreted proteins of Streptococcus equi with potential in vaccine development. (Co-PI: Arti-ushin, S.C.), $354,192.

Timoney, J.F. 2009-2012. Hatch Project. #04-01. Iden-tification of surface and secreted proteins of Streptococ-cus equi with potential in vaccine development. (Co-PI: Artiushin, S.C.).

Tobin, T. 1995-Present. National Horsemen’s Benevo-lent and Protective Association. Synthesis of Certified Reference Standards. Current support: $100,000.

Tobin, T. 2007-2010. KY Commercialization Fund Proposal. Novel therapeutic approaches to treatment of parasitic diseases and related clinical conditions. PI, $150,000.

Tobin, T. 2008-2011. KY Commercialization Fund Pro-posal. Early diagnosis of fescue toxicity. PI, $149,000.

Troedsson, M.H.T. University of Florida competitive research grants. Effects of treatment with a high dose of oral dexamethasone solution on testicular function in miniature horse stallions. Co-PI. $7,500.

Non-Competitive Grant FundingBalasuriya, U.B.R. 2009-2011. Paul Mellon Post Doc-toral Scholarship Award to Dr. Yanqiu Li. Comparative Genomics of Gamma-Herpesviruses of Equids. PI and author. $72,000.

Balasuriya, U.B.R. 2010. Gluck Equine Research Center Intramural Grant Support. Cloning of the full-length neu-ropathogenic equine herpesvirus-1 Findlay strain (T953) genome as an infectious bacterial artificial chromosome (BAC) in E.coli. PI. $6,000.

Balasuriya, U.B.R. 2010. Gluck Equine Research Center

Intramural Grant Support. Discovery of equine genes associated with the susceptibility to equine arteritis virus (EAV) infection of equine CD3+ T lymphocytes and identification of possible genetic marker(s) for the prediction of the establishment of EAV persistent infec-tion in stallions. Co-PI. $7,000.

Balasuriya, U.B.R. 2010. Research Activity Award, College of Agriculture, University of Kentucky. Es-tablishment of the importance of the role of heparin/heparan sulfate as an attachment factor for infection of equine endothelial cells (EECs) with equine arteritis


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Awards Betancourt, Alex. December 2010. “The effect of anthelmintics on proinflammatory cytokine responses in treated horses” by Betancourt, E.T. Lyons, and D.W. Horohov. First place oral presentation at the Conference for Research Workers in Animal Disease in Chicago.

virus (EAV). PI. $1,250.

Chambers, T. 2008-2010. SAIC (NIAID). Testing methods for influenza infection in equine models. Co-PI (PI: Rao, S. NIAID). $156,441.

Chambers, T. 2009-2010. Intervet/Schering-Plough Inc. Does vaccination of very young foals with FluAvert in the presence of maternal antibodies protect them from infection? $47,697.

Cook, R.F. 2009-2010. University of Kentucky, Re-search Activity Awards. Development of an adenoviral vector expressing equine. $1,648.

Cook, R.F. 2009-2010. Gluck Equine Research Center. Molecular Epidemiology and Improved Diagnostics for Equine Infectious Anemia Virus (EIAV). $5,000.

Cook, R.F. 2009-2010. Gluck Equine Research Center. Improved DNA Vaccines: Evaluating Cell Mediated Im-mune Responses. $7,500 (Total: $8,530).

Dwyer, R.M., Williams, N. and Timoney, P.J. 2010. Lloyd’s of London. Continued funding for the Lloyd’s Equine Disease Quarterly. $45,000.

Horohov, D.W., Timoney J.F., Page A., Loynachan A., and Bryant, U. Kohler Funds for newly emerging equine diseases. Development of an infectious model for equine proliferative enteropathy caused by Lawsonia intracellularis. $21,168.

Horohov, D.W. and Adams, A.A. Gluck Equine Re-search Center. The determination of telomerase activ-ity and its relationship with telomere length, oxidative stress and inflammation. $9,630.

Squires, E.L. 2010. Shapiro Endowment. Establish correlation between in vitro susceptibility of CD3+ lymphocytes to EAV infection and the establishment of persistent infection in stallions. Co-PI. $20,000.

Squires, E.L. 2010-2011. Bacteria found in semen and

Non-Competitive Grant Fundingreproductive tracts of stallions and its relation to mare fer-tility using natural service. (Co-PI: Troedsson, M.H.T.)

Squires, E.L. 2010-2011. Performance of foals from mares treated for placentitis during late gestation. (Co-PI: Troedsson, M.H.T.)

Squires, E.L. 2010-2011. Effect of season, age and reproductive status at time of breeding on occurrence of dystocia. (Co-PI: Troedsson, M.H.T.)

Squires, E.L. 2010-2011. The role of nitric oxide in equine post breeding endometritis. Co-PI.

Squires, E.L. 2010-2011. Relationship of endometrial angiopathies to uterine artery rupture. Co-PI.

Timoney, P.J. 2008-2010. Fort Dodge Animal Health Research Agreement. Development and Characterization of Equine Arteritis Virus Vectored Chimeric Viruses as Vaccines against Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). (Co-PI). $211,000, total costs for two years.

Timoney, P.J. 2009-2010. Research Activity Award (RAA). Identification of putative equine arteritis virus (EAV) receptor(s) in equine peripheral blood mononuclear cells. $1,180.50.

Tobin, T. 2009-2011. National and Local Horsemen’s Be-nevolent and Protective Associations. Synthesis of certi-fied reference standards. $91,673.

Tobin, T. 2009-2010. Racing Medication and Testing Consortium. Synthesis of stable isotope reference stan-dards. $23,000.

Troedsson, M.H.T. 2009-2010. Koller Emerging Dis-eases Research Fund, University of Kentucky. Study on the transmission of Taylorella equine genitalis through artificial insemination with semen from infected stallions. (Co-PI: Squires, E.L.); $37,000.


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Scientific Publications

Books/Chapters in Books

Refereed Journal Articles

Non-Refereed Articles

Presentations/Meetings Attended

Timoney, J.F. 2010. Streptococcus. pp. 124-128. IN: C.L. Gyles, J.F. Prescott, J.G. Songer and C.O. Thoen, ed. Patho-genesis of Bacterial Infections in Animals, 4th Ed., Blackwell Publishing, Ames, Iowa.

Timoney, J.F. 2010. Glanders. pp. 638-639. IN: C.M. Kahn and S. Line, ed. The Merck Veterinary Manual, 10th Ed., Merck and Co., Inc., Whitehouse Station, New Jersey.

Timoney, P.J. 2010. Equine viral arteritis. pp. 633-638. IN: C.M. Kahn and S. Line, ed. The Merck Veterinary Manual, 10th Ed., Merck and Co., Inc., Whitehouse Station, NJ.

Troedsson, M.H.T. and P.J. Timoney. 2010. Contagious equine metritis. Section 1. IN: D. Wilson, ed. Clinical Veteri-nary Advisor: The Horse, W.B. Saunders Co.

Research results conducted by the faculty at the Gluck Center was pub-lished in various forms throughout 2010, including books or chapters in books, refereed journal articles and non-refereed articles.

Books/Chapters in Books

Refereed Journal ArticlesAssis A.C., B.A. Ball, P. Browne, A.J. Conley. Cellular Localization of Androgen Synthesis in Equine Granulosa-theca Cell Tumors: Immunohistochemical Expression of 17α-hydroxylase/17,20-lyase Cytochrome P450. Theriogenology 74:393-401, 2010.

Almeida J., A.J. Conley, L. Mathewson L, B.A. Ball. Expression of steroidogenic enzymes during equine testicular development. Reproduction. (In press).

Bailey, C.S., L. Fallon, W. Wang, L. Borst, J.F. Timoney. 2010. Use of a Salmonella-bacterin in pregnant mares to enhance protective antibodies in neonatal foals. Anim Reprod Science 121: 367-368 (Supplement)

Bailey, C.S., M.L. Macpherson, M.A. Pozor, M.H.T. Troedsson, S. Benson, S. Giguere, L.C. Sanchez, M.M. LeBlanc, and T.W. Vicroy. 2010. Treatment efficacy of trimethoprim sulfamethoxazole, pentoxifylline and altrenogest in experi-mentally-induced equine placentitis. Theriogenology 74:402-412.

Bellone R.B., G. Forsyth, T. Leeb , S. Archer , S. Sigurdsson, E. Mauceli, M. Enquensteiner, E. Bailey, L. Sandmeyer, B. Grahn. 2010. Fine mapping and mutation analysis of TRPM1, a candidate gene for Leopard Complex (LP) spotting and congential stationary Night Blindness (CNSB) in horses and fine mapping the Leopard Complex (LP) spotting gene and congenital stationary night blindness (CSNB) in horses. Briefings in Functional Genomics and Proteomics 9:193-207.

Binns M., Boehler D., Bailey E., Lear T., Cardwell J., Lambert D. 2010. Inbreeding in the Thoroughbred horse. Animal Genetics (In press).

Boliar, S., and T.M. Chambers. 2010. A new strategy of immune evasion by influenza A virus: inhibition of monocyte differentiation into dendritic cells. Veterinary Immunology and Immunopathology 136:201-210.

Brault, S.A., B.H. Bird, U.B.R. Balasuriya, and N.J. MacLachlan. 2010. Genetic heterogeneity and variation in viral load during equid herpesvirus-2 infection of foals. Veterinary Microbiology 147(3-4):253-261.

Brooks, S.A., and E. Bailey. 2010. RT-qPCR comparison of mast cell populations in whole blood from healthy horses and those with laminitis. Animal Genetics 41 (supplement 2):16-22.

Cappelli K., S. Capomaccio, R. F. Cook, M. Felicetti, M. L. Marenzoni, G. Coppola, A. Verini-Supplizi, M. Coletti and F. Passamonti. 2010. Molecular diagnosis, epidemiology and genetic characterization of novel European field isolates of


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equine infectious anemia virus. J. Clin. Micro. (In press).

Causey, R.C., S.C. Artiushin, I.F. Crowley, J.A. Weber, A.D. Homola, A. Kelley, L.A. Stephenson, H.M. Opitz, S. Guil-main, and J.F. Timoney. 2010. Immunization of the equine uterus against Streptococcus equi subspecies zooepidemicus using an intranasal attenuated Salmonella vector. Veterinary Journal 184:156-161.

Coleman, S.J., Z. Zeng, K. Wang, S. Luo, I. Khrebtukova, M.J. Mienaltowski, G.P. Schroth, J. Liu, and J.N. MacLeod. 2010. Structural annotation of equine protein-coding genes determined by mRNA sequencing. Animal Genetics 41 (Suppl. 2):121–130.

Cook D., P.C. Gallagher and E. Bailey. 2010 Genetics of swayback in American Saddlebred Horses. Animal Genetics 41 (supplement 2):64-71.

Craigo J.K., S. Barnes, S.J. Cook, C.J. Issel, R.C. Montelaro. 2010. Divergence not diversity of an attenuated equine lentivirus vaccine strain correlates with protection from disease. Vaccine 28:8095-8104.

Cullingford, E.L., E.L. Squires, P.M. McCue, and G.E. Seidel, Jr. 2010. Attempts at superovulation of mares with porcine follicle stimulating hormone and recombinant equine follicle stimulating hormone. Journal of Equine Veterinary Science 30(6):305-309.

Dawson, T.R.M.Y., D.W. Horohov, W.G. Meijer, and G. Muscatello. 2010. Current understanding of the equine immune response to Rhodococcus equi. An immunological review of R. equi pneumonia. Veterinary Immunology & Immunopa-thology 135:1-11.

Diaz-Mendez, A, S. Carman, A. Tiwari, T. Chambers, J. Hewson, C. Dewey, and L. Viel. 2010. Surveillance of equine influenza virus in Ontario horses. Canadian Journal of Veterinary Research 74:271-278.

Go Y.Y., E.J. Snijder, P.J. Timoney and U.B.R. Balasuriya. Characterization of Equine Humoral Antibody Response to the Nonstructural Proteins of Equine Arteritis Virus. Clin Vaccine Immunol. (In press).

Go, Y.Y., J. Zhang, P.J. Timoney, R.F. Cook, D.W. Horohov and U.B.R.Balasuriya. 2010. Complex interactions be-tween the major and minor envelope proteins of equine arteritis virus determine its tropism for equine CD3+ T lympho-cytes and CD14+ monocytes. Journal of Virology 84(10):4898-4911.

Gutierrez, J., R. Eisenberg, G. Herrensmith, T. Tobin, T. Li and S. Long. 2010. Conformational polymorphism in 2-((4-hydroxy-2,6-dimethylphenyl) amino)-5,6-dihydro-4H-1,3-thiazin-3-ium chloride. Acta Crystallographica Section C: Crystal Structure Communications C66:593-595.

Gutierrez J, R.L. Eisenberg, N.J. Koval, E.R. Armstrong, J. Tharappel, C.G. Hughes, T. Tobin. 2010. Pemoline and Tet-ramisole Positives In English Racehorses Following Levamisole Administration. Irish Vet J. 63 (8) 498-500.

Holl, H., S. Brooks, and E. Bailey. 2010. De novo mutation of KIT discovered as a result of non-hereditary white coat color pattern. Animal Genetics 41(supplement 2):196-198.

Horohov, D.W., Adams, A.A., and Chambers, T.M. 2010. Immunosenescence of the equine immune system. Journal of Comparative Pathology 142: S78-S84.

Ijaz, M., S. Velineni, and J.F. Timoney. 2010. Selective pressure for allelic diversity in SeM of Streptococcus equi does not affect immunoreactive proteins SzPSe or Se18.9. Infection, Genetics and Evolution.

Ionita, M., D.K. Howe, E.T. Lyons, S.C. Tolliver, R.M. Kaplan, I. L. Mitrea, M. Yeargan. 2010. Use of a Reverse Line Blot assay to survey small strongyle (Strongylida: Cyathostominae) populations in horses before and after treatment with ivermectin. Veterinary Parasitology 168:332-337.

Klein C., Donahue J.M., Sells S.F., Squires E..L, Timoney P.J., Troedsson M.H.T. 2010. Antibiotic-containing semen extender reduces the risk of dissemination of CEM. JAVMA (In press).

Klein, C., K. Scoggin, A.D. Ealy, and M.H.T. Troedsson. 2010. Transcriptional profiling of equine endometrium during the time of maternal recognition of pregnancy. Biology of Reproduction 83:102-113.

Klein, C., K. Scoggin, and M.H.T. Troedsson. 2010. The expression of Inteferon stimulated gene 15 in equine endome-Research Report 2010 •

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Refereed Journal Articlestrium. Reprod. Domest. Anim. (In press).

Kuzmina, T.A., S.C. Tolliver, and E.T. Lyons. Three recently recognized species of cyathostomes (Nematoda: Strongyl-idae) in equids in Kentucky. Parasitology Research (Published On Line on November 24, 2010; DOI 10.1007/s00436-010-2160-z).

Lin, C.B., R.E. Holland, J.C. Donofrio, M.H. McCoy, and T.M. Chambers. 2010. Infectivity of equine influenza virus in bovine turbinate cells and cattle. Influenza and Other Respiratory Viruses 4: 357-361.

Lu, Z., E.J. Dubovi, N. Zylich, P.C. Crawford, S. Sells, Y.Y. Go, A.T. Loynachan, P.J. Timoney, T.M. Chambers, T. M., and U.B.R. Balasuriya. 2010. Diagnostic application of H3N8 specific equine influenza real-time RT-PCR as-says for the detection of canine influenza virus in clinical specimens. Journal of Veterinary Diagnostic Investigation 22(6):942-945.

Lyons, E.T., S.C. Tolliver, T.A. Kuzmina, and S.S. Collins. 2010. Critical tests evaluating efficacy of moxidectin against small strongyles in horses from a herd for which reduced activity had been found in field tests in Central Kentucky. Parasitology Research 107:1495–1498.

Lyons, E.T., S.C. Tolliver, S.S. Collins, M. Ionita, T.A.Kuzmina, and M. Rossano. Field tests demonstrating reduced activity of ivermectin and moxidectin against small strongyles in horses on 14 farms in Central Kentucky in 2007–2009. Parasitology Research (Published On Line September 23, 2010; DOI 10.1007/s00436-010-2068-7).

McDowell, K.J., B.A. Webb, N.M. Williams, J.M. Donahue, K.E. Newman, M.D. Lindemann, and D.W. Horohov. 2010. Editorial Board Invited Review: The role of caterpillars in mare reproductive loss syndrome – a model for envi-ronmental causes of abortion. Journal of Animal Science 88:1379-1387.

Meade, B.J., P.J. Timoney, J.M. Donahue, A.J. Branscum, R. Ford and R. Rowe. 2010. Initial occurrence of Taylorella asinigenitalis and its detection in nurse mares, a stallion and donkeys in Kentucky. Preventive Veterinary Medicine 95:292-296.

Meyers-Brown, G.A., P.M. McCue, K.D. Niswender, E.L. Squires, C.A. DeLuca, L.A. Bidstrup, M. Colgin, T.R. Famula, and J.F. Roser. 2010. Superovulation in mares using recombinant equine follicle stimulating hormone: ovula-tion rates, embryo retrieval and hormone profiles. Journal of Equine Veterinary Science 30(10):560-568.

Mienaltowski, M.J., L. Huang, A. Bathke, A.J. Stromberg, and J.N. MacLeod. 2010. Transcriptional comparisons between articular repair tissue, neonatal cartilage, cultured chondrocytes, and mesenchymal stromal cells. Briefings in Functional Genomics & Proteomics 9:238-250.

Moller-Nielsen, J., M.H.T. Troedsson, and W.W. Zent. 2010. Results of bacteriological and cytological examinations of the endometrium of mares in a practice in Denmark and in Central Kentucky, USA. J of Equine Veterinary Science 30(1):27-30.

Mozzaquatro, F.D., J.P. Verstegen, R.H. Douglas, M.H.T. Troedsson, C.A.M. Silva, and M.I.B. Rubin. 2010. Luteal function induced by transvaginal ultrasonic-guided follicular aspiration in mares. Animal Reproduction Science 119(1-2):56-62.

Natarajaseenivasan, K., K. Vedhagiri, V. Sivablan, S.G. Prabagaran, S. Sukumar, S.C. Artiushin and J.F. Timoney. 2010. Seroprevalence of Leptospira borgpetersenii serovar Javanica infection among dairy cattle, rats and humans in the Cauvery river valley of southern India. South East Asian J. Trop. Med. and Public Health. (In press).

Nielsen, M.K., K.E. Baptiste, S.C. Tolliver, S.C. Collins, and E.T. Lyons. 2010. Analysis of multiyear studies in horses in Kentucky to ascertain whether counts of eggs and larvae per gram of feces are reliable indicators of numbers of stron-gyles and ascarids present. Veterinary Parasitology 174:77–84.

Pronost, S., P.H. Pitel, F. Miszczak, L. Legrant, C. Marcillaud-Pitel, M. Hamon, J. Tapprest, U.B.R. Balasuriya, F. Freymuth, and G. Fortier. 2010. Description of the first recorded major occurrence of equine viral arteritis in France. Equine Veterinary Journal 42(8):713-720.


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Refereed Journal ArticlesPronost, S., R.F. Cook, G. Fortier, P.J. Timoney, and U.B.R. Balasuriya. 2010. Relationship between equine herpesvi-rus-1 myeloencephalopathy and viral genotypes. Equine Veterinary Journal 42(8):672-674.

Raudsepp, T., K. Durkin, T.L. Lear, P.J. Das, F. Avila, P. Kachroo, B.P. Chowdhary. 2010. Molecular heterogeneity of XY sex reversal in horses. Animal Genetics 41 (suppl 2):41-52.

Rossano M.G, A.R. Smith, and E.T. Lyons. 2010. Shortened strongyle-type egg reappearance periods in naturally in-fected horses treated with moxidectin and failure of a larvicidal dose of fenbendazole to reduce fecal egg counts. Veteri-nary Parasitology 173:349-352.

Saulez, M.N., J. Godfroid, A. Bosman, J.L. Stiltner, C.C. Breathnach, and D.W. Horohov. 2010. Cytokine mRNA ex-pressions after racing at a high altitude and at sea level in horses with exercise-induced pulmonary hemorrhage. Ameri-can Journal of Veterinary Research 71:447-453.

Smith, K.L., G.P. Allen, A.J. Branscum, R. F. Cook, M. L. Vickers, P. J. Timoney, and U.B.R. Balasuriya. 2010. In-creased prevalence of neuropathogenic strains of EHV-1 in equine abortions. Veterinary Microbiology 141:5-11.

Spizziri, B.E., M.H. Fox, J.E. Bruemmer, E.L. Squires, and J.K. Graham. 2010. Cholesterol-loaded cyclodextrins and fertility potential of stallions spermatozoa. Animal Reproduction Science 118(2-4):255-264.

Sturgill, T.L., and D.W. Horohov. 2010. Vaccination response of young foals to Keyhole Limpet Hemocyanin: Evidence of effective priming in the presence of maternal antibodies. Journal of Equine Veterinary Science 30:359-364.

Timoney P.J. 2010. Contagious Equine Metritis: An Insidious Threat to the US Horse Breeding Industry. J Anim Sci. (In press).

Tompkins, D., E. Hudgens, D. Horohov, and C.L. Baldwin. 2010. Expressed gene sequences of the equine cytokines interleukin-17 and interleukin-23. Veterinary Immunology & Immunopathology 133:309-313.

Troedsson, M.H.T. 2010. Sperm transport and elimination from the mares’ reproductive tract. Pferdeheilkunde 26:25-28.

Tseng, C.T., D. Miller, J. Cassano, E. Bailey and D.F. Antczak. 2010. Molecular identification of equine major histo-compatibility complex haplotypes using polymorphic microsatellites. Animal Genetics 41 (supplement 2):150-153.

Verma, A., P. Kumar, K. Babb, J.F. Timoney and B. Stevenson. 2010. Cross-reactivity of antibodies against leptospiral recurrent uveitis-associated proteins A and B (Lru A and Lru B) with eye proteins. PLoS Neglected Tropical Diseases 4(8):e778.

Villagómez D.A.F., T.L. Lear, T. Chenier, S. Lee, R.B. McGee, J. Cahill, R.A. Foster, E. Reyes, E. St John, W.A. King. 2010. Equine disorders of sexual development in 17 mares including XX SRY-negative, XY SRY-negative and XY SRY-positive genotypes. Sexual Development, published online, Dec. 22.

Wang K, D. Singh, Z. Zeng, S.J. Coleman, Y. Huang, G.L. Savich, H. Xiaping, P. Mieczkowski, S.A. Grimm, C.M. Perou, J.N. MacLeod, D.Y. Chiang, J.F. Prins, and J. Liu. 2010 MapSplice: accurate mapping of RNA-seq reads for splice junction discovery. Nucleic Acids Research 38(18):e178.

Zhang, J., D.A. Stein, P.J. Timoney, and U.B.R. Balasuriya. 2010. Cure of persistent equine arteritis virus infection in HeLa cells by treatment with a peptide-conjugated morpholino oligomer. Virus Research 150(1-2):138-142.

Zhang, J., P.J.Timoney, K.M. Shuck, G. Seoul, Y.Y. Go, Z. Lu, D.G. Powell, B.J. Meade, and U.B.R. Balasuriya. 2010. Molecular epidemiology and genetic characterization of equine arteritis virus isolates associated with the 2006-2007 multi-state disease occurrence in the USA. Journal of General Virology 91:2286-2301.


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Non-Refereed ArticlesAlmeida J.A., A.J. Conley, L. Mathewson, B.A. Ball. 2010. Role of anti-Müllerian hormone, CDKN1B and the andro-gen receptor in regulation of pubertal development of the stallion testis. An Reprod Sci 121:139-140.

Bailey, E. 2010. Horse genomics and the Dorothy Russell Havemeyer Foundation. Animal Genetics 41 (supplement 2):1.

Bailey, E and J.N. MacLeod. 2010. White Paper to USDA: Equine Genome Reference Sequence Refinement. November 1.

Blanes M.S., Y. Nambo, K. Taya, L. Mathewson, B.A. Ball. 2010. Expression of anti-Müllerian hormone (AMH) and its receptor in equine endometrium and placenta. An Reprod Sci 121:318-319.

Bruemmer, J.E., G.J. Bouma, A. Hess, T.R. Hansen, and E.L. Squires. 2010. Gene expression in the equine endome-trium during maternal recognition of pregnancy. Ani Reprod Sci. 121S (2010) S286-S287.

Camargo, F. and R.M. Dwyer. 2010. Evaluating the Health of your Horse ID 179. UK Extension Publication.

Coleman, S.J., Z. Zeng, D. Miller, C. Klein, M.H.T. Troedsson, D.F. Antczak, J. Liu, and J.N. MacLeod. 2010. Gener-ation of a consensus protein-coding equine gene set. P619. Proceedings, Plant and Animal Genome XVIII Conference, San Diego, CA, January 9-13.

Cook, D.G. and E. Bailey. 2010. Genetics of Extreme Lordosis in American Saddlebred Horses. Plant and Animal Ge-nome Conference XVIII (P621), p 222.

Diaw, M., C.S. Bailey, D. Schlafer, M. Pozor, M.H.T. Troedsson, S. Benson, and M.L. Macpherson. 2010. Evaluation of endometrial culture and biopsy immediately after post partum from normal mares and those with induced placentitis. Ani Reprod Sci. 121S:369-370.

Dobrinsky, J.R., P. Griffin, A.M. Paprochi, R.W. Koppang, C.M. Syverson, C. Klein, and M.H.T. Troedsson. 2010. Transfer success of biopsied and vitrified equine embryos. Ani Reprod Sci. 121S:295-296.

Dwyer, R.M. 2010. Adverse effects. Lloyd’s Equine Disease Quarterly 19:3.

Dwyer, R.M. 2010. Biosecurity for horse farms: Stall disinfection and other management techniques. Bluegrass Equine Digest. www.thehorse.com article 16350.

Dwyer, R.M. 2010. Commentary. Lloyd’s Equine Disease Quarterly 19: 1.

Dwyer, R.M. 2010. Rabies in the US and Puerto Rico. Lloyd’s Equine Disease Quarterly 19:3.

Dwyer, R.M., et. al. 2010. Horse Transmitted Diseases brochure (12 pages). UK HealthCare/Saddle Up Safely Consortium.

Dwyer, R.M. 2010. State specific equine vaccination recommendations? Kentucky Horse Council online newsletter. http://www.kentuckyhorse.org/en/art/642/

Eberth J. and E. Bailey. 2010. Genetics of dwarfism in Miniature Horses. ISAG, Edinburgh, Scotland. July 25-30.

Faaberg, K. S., U.B.R. Balasuriya, A.E. Gorbalenya, and E.J. Snijder, Chapter 03.004.0.01.001. Equine arteritis virus. In A.J. Davison, et al. (chair), Vetebrate Virus Subcommittee. International Committee on Taxonomy of Viruses (ICTV) Online Database. http://www.ictvdb.org/.

Gee, E.K., J.E. Bruemmer, P.D. Siciliano, P.C.H. Morel, T.E. Engle, P.M. McCue, and E.L. Squires. 2010. Effects on spermatozoa of dietary supplementation of vitamin E and omega-3 fatty acids in stallions with poor post-thaw motility. Ani Reprod Sci.121S:S206-S207.


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Go, Y.Y., E. Bailey, D. Cook, P.J. Timoney, R.F. Cook, and U.B.R. Balasuriya. 2010. Genome-wide scan to identify genetic factors responsible for the susceptibility of horses to equine arteritis virus infection. Proceedings, 9th Interna-tional Symposium on Positive Stranded RNA Viruses, Atlanta, GA, May 17th-21st.

Go, Y.Y., P.J. Timoney, E.J. Snijder and U.B.R. Balasuriya. 2010. Characterization of equine humoral antibody re-sponse to the nonstructural proteins of equine arteritis virus. Proceedings, Conference of Research Workers in Animal Disease, Chicago, IL, Dec. 5th-7th.

Graves, KT, 2010. Neonatal Isoerythrolysis. Equine Disease Quarterly 19:1.

Gutierrez, J., R. Eisenberg, G. Herrensmith, T. Tobin, T. Li and S. Long. 2010. Conformational Polymorphism in 2-([4-hydroxy-2,6-dimethylphenyl] amino)-5,6-dihydro-4H-1,3-thiazin-3-ium Chloride, C66, p 593-595, Acta Crystal-lographica Section C: Crystal Structure Communications. Ky Ag Expt Station Numberr 10-14-123 UK #392.

Gutierrez J., R. Eisenberg, G. Herrensmith, T.Tobin, T. Li, S. Long. Solvatomorphism in (E)-2-(2,6-dichloro-4-hy-droxybenzylidene) hydrazinecarboximidamideActa Crystallographica Reference: sq3285. UK# 394. (In press).

Higgins S. and R.M. Dwyer. October 2010. Water Quality Testing. Lloyd’s Equine Disease Quarterly.

Husband, R. R. Yeargan, R.M. Dwyer, and M. Newman (2010) EDEN Strengthening Community Agrosecurity Plan-ning Workshop Instructor Guide (national teaching guide), 430 pages.

Klein, C., J.M. Donahue, S.F. Sells, E.L. Squires, P.J. Timoney, and M.H.T. Troedsson. 2010. Antibiotic-containing semen extender reduces the risk of transmission of CEM. Ani Reprod Sci. 121S:S222-S223.

Klein, C., A. Ealy, K. Scoggins, and M.H.T. Troedsson. 2010. Transcriptive profiling of equine endometrium during the time of maternal recognition of pregnancy. p. 229. Proceedings, Animal Conference of the International Embryo Transfer Society, Cordoba, Argentina, Jan. 9-12.

Klotz, J.L., and K.J. McDowell. 2010. Tall fescue alkaloids cause vasoconstriction in equine medial palmar artery and vein. Journal of Animal Science 88 (E-Suppl 2):55.

Lehnera, A.F., J.M. Duringer, C.T. Estill, T. Tobin, A. Morrie Craig. 2010. ESI-Mass spectrometric and HPLC elucida-tion of a new ergot alkaloid from perennial ryegrass hay silage associated with bovine reproductive problems, Toxicol-ogy Mechanisms and Methods, KAES Article # 11-14-031 UK # 396. (In press).

Lu, K., W. Zent, S. Hughes, J. Roser, and E.L. Squires. 2010. Clinical use of recombinant FSH in mares. Clinical The-riogenology 2(3):398.

Lu, Z., P.J. Timoney, H.W. Heidner, J.C. Williams, J. Zhang, and U.B.R. Balasuriya. 2010. Heparin-dependent attach-ment of equine arteritis virus to equine endothelial cells. Proceedings, 9th International Symposium on Positive Strand-ed RNA Viruses, Atlanta, GA, May 17th-21st.

McDowell, K.J., T.V. Little, and C.B. Baker. 2010. Models to predict reproductive success in horses. Ani Reprod Sci.121S:126-127.

McGee, R.B., T.L. Lear, K.T. Graves, E. Bailey. 2010. XY sex reversal in mares. Plant and Animal Genome Confer-ence XVIII (P629), p 223.

McGee, R.B. and T.L. Lear. 2010. Diagnosing disorders of sexual development in horses. Bluegrass Equine Digest. October.

Meade, B. and P.J. Timoney. 2010. Monitoring and surveillance of equine infectious diseases. Equine Disease Quar-terly 19(1):4.


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Non-Refereed ArticlesMiller, L.M.J., E. Woodward, J.R. Campos, and M.H.T. Troedsson. 2010. Expression of sperm protein at 22 kDa (SP22) in equine spermatozoa prior to and following heat induced testicular degeneration. Ani Reprod Sci. 121S:148.

Miszczak, F., Z. Lu, K.M. Shuck, P.J. Timoney, Y.Y. Go, J. Zhang, S. Sells, A. Vabret, S. Pronost, A.J. Branscum, and U.B.R. Balasuriya. 2010. Comparison and optimization of two previously described real-time RT-PCR assays for the detection of equine arteritis virus in equine semen samples. Proceedings, 53rd Annual AAVLD/USAHA Meeting, Minne-apolis, MN, Nov. 11th-17th.

Miszczak, F., Z. Lu, K.M. Shuck, P.J. Timoney, Y.Y. Go, J. Zhang, S. Sells, A.J. Branscum, L. Legrand, A. Vabret, S. Pronost, and U.B.R. Balasuriya. 2010. Comparison of commercial nucleic acid purification and real-time RT-PCR ampli-fication kits for the detection of equine arteritis virus in equine semen suing two previously described one-step real-time RT-PCR assays. Proceedings, 1st Congress of the European Association of Laboratory Diagnosticians (EAVLD), Lelystad, The Netherlands, September 15th-17th.

Morganti M., A.J. Conley, A.E. Vico, C.J. Munro, B.A. Ball. 2010. Stallion-like behavior in mares: What is the role of the adrenal glands? Proc. Am. Assoc. Equine Pract.

Mozzaquatro F.D, J.P. Verstegen, R.H. Douglas, M.H.T. Troedsson, F.D. De la Corte, M.I.B. Rubin, C.A.M. Silva. 2010. Progesterone profile secretion by Corpus luteum formed after follicular aspiration. 2nd Symposium on Animal Reproduc-tion.

Pozor, M., M.H.T. Troedsson, M.L. Macpherson, and R. Sams. 2010. Effects of treatment with oral dexamethasone solu-tion on testicular function in miniature horse stallions. Animal Reproduction Science 121S:141-142.

Scholtz E.L., B.A. Ball, S.D. Stanley, B.C. Moeller, A.J. Wirz, A.J. Conley. 2010. 5α-dihydroprogesterone is bioactive in the horse. An Reprod Sci 121:229-230.

Spizziri, B.E., N. Kaula, E.L. Squires, and J.K. Graham. 2010. In vitro capacitation of stallion spermatozoa. Ani Reprod Sci.121S:S181-S183.

Stoll A., C.C. Love, B.A. Ball. 2010. Use of a single layer density centrifugation to reduce the number of DNA-damaged sperm and enhance sperm quality in cryopreserved-thawed stallion sperm. An Reprod Sci 121:163-164.

Timoney, P.J. 2010. Globalisation of trade and the increased risk of spread of equine diseases. Irish Veterinary Journal 63:210-212.

Troedsson, M.H.T. 2010. Sperm transport, elimination and endometritis. Clinical Theriogenology 2(3):320-325.

Troedsson, M.H.T. 2010. Management of the problem mare: Uterine issues. Pp. 10-15. Proceedings, Equine Reproduc-tion, Conference for Veterinarians: Current topics on broodmare management, Columbus, OH, Feb. 6-7, 2010.

Troedsson, M.H.T., A. Doty, M.L. Macpherson, M.C. Connor, J.P. Verstegen, M.A. Pozor, and W.C. Buhi. 2010. CRISP-3 in equine seminal plasma is involved in selective uterine sperm transport. Ani Reprod Sci. 121S:192-193.

Troedsson, M.H.T., A. Doty, K. Scoggin, and W.C. Buhi. 2010. Sperm transport and elimination from the mares’ repro-ductive tract. Pferdeheilkunde 26(1):25-28.

Vazquez J.J., A. Garcia, P.H. Kass, I.K.M. Liu, B.A. Ball. 2010. Influence of environmental temperature, exercise, fro-zen semen and ovulation type on reproductive performance in a commercial embryo transfer program. An Reprod Sci 121:301-302.

Zhang, J., Y.Y. Go, Z. Lu, B.J. Meade, P.J. Timoney, and U.B.R. Balasuriya. 2010. Development and characterization of an infectious cDNA clone of the modified live virus vaccine strain of equine arteritis virus and its potential as a vaccine vector. Proceedings, 9th International Symposium on Positive Stranded RNA Viruses, Atlanta, GA, May 17th-21st.


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Faculty at the Gluck Equine Research Center are frequent travelers. They are guest speakers at veterinary conferences and meetings locally, nationally and internationally in their respected fields throughout the year.

Presentations/Meetings Attended

INTERNATIONALErnie Bailey. Poster presentation on Dwarfism in miniature horses, International Society of Animal Genetics. Edinburgh, Scotland, July 24-30

Ernie Bailey. Chair Equine Genetics Section, World Congress on Genetics Applied to Livestock Produc-tion. Leipzig, Germany. Aug.1-6

Barry Ball. Anti-Mullerian Hormone: A new en-docrine marker for the mare. Tokyo University of Agriculture and Technology, Japan.

Barry Ball. Diagnostic methods for examination of the stallion. Japanese Racing Association, Hidaka, Hokkaido, Japan.

Barry Ball. “Ultrasonographic and endoscopic ex-aminations of stallions’ reproductive organs,” “En-docrinological evaluation of prospective and active breeding stallions,” “Diseases of the scrotum and testis,” “Testicular biopsy in the stallion,” “Sperm transit and storage in the mare reproductive tract,” “Oxidative stress in normal and abnormal function of equine spermatozoa,” “Sperm motility, morphology and viability: research with aims to improve.” Ross-dales Stallion Subfertility Course, Newmarket, UK.

Kathy Graves. International Society for Animal Ge-netics. Edinburgh, Scotland. July 26-30

Charles Issel. “Equine infectious anemia and equine infectious diseases.” Presented to the faculty and students at the University of Agronomic Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, Bucharest, Romania, Dec. 14

Charles Issel. “Equine infectious anemia: the chal-lenges remaining.” Presented to the faculty and stu-dents at the University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, Cluj-Napoca, Romania, Dec. 15

Charles Issel. “Equine infectious anemia: control or

eradication?” Presented to the Romanian Veterinary As-sociation Meeting, Cluj-Napoca, Romania, Dec. 17

John Timoney. “Getting to Grips with Strangles.” Invited speaker and chair of workshop on Strangles Vaccine Development. The two-day conference and workshop was organized by the Animal Health Trust. Stockholm, Sweden, May 27-28

John Timoney and S. Velineni. “Now you see it, now you don’t. Regulation of capsule and protein expression in Streptococcus zooepidemicus.” The Prato Confer-ence on the Pathogenesis of Bacterial Diseases of Animals. Prato, Italy. Oct. 6-9

Peter Timoney. Participated in Second International Meeting of PrEquID, Prevention of Equine Infectious Diseases Group, Marrakech, Morocco. March 23-27

Peter Timoney. Invited visitor to meet with members of the National Animal Health Authority (SENASA), equine practitioners, horse owners and breeders, labora-tory workers and to give presentations and discuss how best to control the outbreak of equine viral arteritis that had occurred, Buenos Aires, Argentina, May 25-28

Peter Timoney. Attended 2nd International Conference of OIE Reference Specialists and Heads of OIE Col-laborating Centres, Paris, France. June 19-24

Peter Timoney. Special Meeting on Equine Viral Arte-ritis, SENASA, Buenos Aires.

Peter Timoney. Advisory Commission to SENASA on Animal Health Issues, Seminar on Equine Viral Arteri-tis. Buenos Aires.

Peter Timoney. Special Meeting on Equine Viral Ar-teritis, Argentinean Equine Practitioners, Owners and Breeders, San Isidro Racetrack. Buenos Aires.

Peter Timoney. Meeting on Current Equine Viral Situ-ation in Argentina, Board of the Argentinean Equine


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Veterinary Association. Buenos Aires.

Peter Timoney. Roundtable on Equine Infectious Diseases organized by Italian Equine Sports Medicine Veterinarian. Rome, Italy.

Peter Timoney. Annual Meeting of Societa Italiana Veterinari per Equini, Milan, Italy.

Peter Timoney. Guandong Entry and Exit Inspection and Quarantine Bureau, Guangzhou, Guandong, PR China.

Peter Timoney. Guangzhou Animal Health Inspection Institute. Guangzhou, Guandong, PR China.

Thomas Tobin. “Certified Reference Standards and Stable Isotope Internal Standard “The Role Of Expert Testimony In Adversarial Proceedings.” Oral presenta-tion at International Conference of Racing Analysts and Veterinarians, Queenstown, New Zealand, March 7-13

Mats Troedsson. “Is sperm transport and elimination a selective process or random event?” Leipzig Workshop on Equine Reproduction, Leipzig, Germany. Jan. 19-20

Mats Troedsson. Leipziger Tierarztekongress, Leipzig, Germany, Jan. 21-23

Mats Troedsson. Wet lab for monitoring of high-risk mares, Helsingborg, Sweden, Jan. 25-27

Mats Troedsson. Keynote speaker, “Breeding-induced endometritis,” “Bacterial endometritis,” “Sperm transport and elimination,” “Embryo transfer and cryopreservation of embryos,” “Pre-implantation genetic testing,” “High-risk pregnancies,” and “Les-sons learned from a recent outbreak of CEM in the US.” Italian Association of Equine Practitioners, SKIVE Equine Veterinary Resort Symposium. De-cember

Miszczak, F., Lu, Z., Shuck, K. M., Timoney, P. J., Go, Y. Y., Zhang, J., Sells, S., Branscum, A. J., Legrand, L., Vabret, A., Pronost, S. and Balasuriya, U. B. R. “Comparison of commercial nucleic acid purification and real-time RT-PCR amplification kits for the detection of equine arteritis virus in equine semen suing two previously described one-step real-time RT-PCR assays.” 1st Congress of the European Association of Laboratory Diagnosticians (EAVLD), Lelystad, The Netherlands. Sept. 15-17

Ernie Bailey. Poster presentation and participate in horse technical committee and executive committee meetings of NRSP8. Plant & Animal Genome XVIII Conference, San Diego, January

Ernie Bailey. “Future of Research on Animal Genet-ics, Genomics and Bioinformatics.” Participant in discussion at the USDA-NIFA, Washington, DC. Sept. 9-10

Ernie Bailey. “Messages to Breeders written in DNA,” National Breeders Conference of the Pyramid Arabian Horse Society. Atlanta. Sept.16-19

Ernie Bailey. Large Animal Scientific Advisory Board, Morris Animal Foundation. Dallas. Oct. 14-16

Ernie Bailey. External Review Committee, National Science Foundation Peromyscus Stock Center. Colum-bia, SC. Nov. 12

Udeni Balasuriya. “Rapid Detection of Foreign, Emerging and Zoonotic Pathogens of Equines.” Center

of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD), Kansas State University, Man-hattan, KS. July 28-29

Udeni Balasuriya. “Equine Viral Arteritis: A disease of evolving significance in an area of expanding trade in horses and semen.” Department of Veteri-nary Science, South Dakota State University, Brook-ings, SD. Oct. 8

Barry Ball. Society for Theriogenology. Seattle, WA. Aug. 31- Sept. 5

Thomas Chambers. Research Advisory Commit-tee, Grayson Jockey Club Research Foundation, Fort Worth, TX. Jan. 8-10

Roberta Dwyer. “Strengthening Agrosecurity Plan-ning for Communities.” Co-presenter and one of the lead trainers at the two-day workshop in Honolulu and Hilo, HI February

Go, Y. Y., Bailey, E., Cook, D., Timoney, P. J.,

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Cook, R. F., and Balasuriya, U. B. R. “Genome-Wide Scan to Identify Genetic Factors Responsible for the Susceptibility of Horses to Equine Arteritis Virus Infection.” 9th International Symposium on Positive Stranded RNA Viruses, Atlanta. May 17-21

Go, Y. Y., Timoney, P.J., Snijder, E.J., and Balasuriya,U.B.R. “Characterization of equine hu-moral antibody response to the nonstructural proteins of equine arteritis virus.” Conference of Research Workers in Animal Disease, Chicago. Dec. 5-7

David Horohov. “Divergent immune development in foals and its implication for susceptibility of dis-ease,” Danisco Animal Nutrition Technical Sympo-sium, Baltimore, MD, March 24

David Horohov. “Parasite Immunology,” “Foal im-mune response to FluAvert” and “Adverse reactions to vaccines.” Intevet/Schering Plough Equine Meet-ing, Kansas City, MO. June 28

David Horohov. “Immunosenescence in the horse,” Havemeyer Foundation Geriatric Equine Workshop. Boston, MA. Oct. 22

Howe, D.K., C.L. Schardl, and J.C. Kissinger. “A genome sequence for the apicomplexan Sarcocystis neurona.” Microbial Genome Sequencing and Mi-crobial Observatories Programs Workshop, Plant and Animal Genomes Conference, San Diego, CA, January

Lu, Z., Timoney. P. J., Heidner, H. W., Williams, J. C., Zhang, J., and Balasuriya, U. B. R. “Heparin-dependent attachment of equine arteritis virus to equine endothelial cells.” 9th International Sym-posium on Positive Stranded RNA Viruses, Atlanta. May 17-21

James N. MacLeod. “Analyses of the equine mRNA transcriptome using RNA-seq data.” Plant & Animal Genome international conference. San Diego, Jan. 10

James N. MacLeod. Equine articular cartilage matu-ration and repair: new technologies and models to address longstanding research questions. University of Minnesota. St. Paul, MN.

Karen McDowell. “Of Caterpillars and Horses: What Happened in Mare Reproductive Loss Syn-

drome and What We Learned.” Invited speaker at the 2nd Annual Woods Lane Farm Equine Reproduction Management Short Course, Mt. Airy, MD, Sept. 16

Miszczak, F., Lu, Z., Shuck, K. M., Timoney, P. J., Go, Y. Y., Zhang, J., Sells, S., Vabret, A., Pronost, S., Branscum, A. J., and Balasuriya, U. B. R. “Com-parison and optimization of two previously described real-time RT-PCR assays for the detection of equine arteritis virus in equine semen samples.” 53rd Annual AAVLD/USAHA Meeting, Minneapolis, MN. Nov. 11-17

Reed, S.M., D.K. Howe, M.R. Yeargan, J.K. Morrow, A.J. Graves, and W.J.A. Saville. “New quantitative assays for the differential diagnosis of equine protozoal myeloencephalitis (EPM).” Late Breaking Research Reports in Neurology, American College of Veterinary Internal Medicine (ACVIM), Anaheim, CA. June

Reed, S.M., D.K. Howe, M.R. Yeargan, J.K. Mor-row, A.J. Graves, and W.J.A. Saville. “Measurement of antibodies against Sarcocystis neurona antigens for the differential diagnosis of equine protozoal myeloen-cephalitis (EPM).” Special Interest Group session on EPM, American College of Veterinary Internal Medi-cine (ACVIM), Anaheim, Calif., June

Edward Squires. Presentation at the Preservation of Equine Genetics Program, Colorado State University, Ft. Collins, CO. Feb. 18

Edward Squires. Stud book and registration commit-tee, American Quarter Horse Convention, Orlando, FL, March 5-8

Edward Squires. Consultant visit, Aspen Bio Science, Castle Rock, CO. Feb. 9

Edward Squires. “Emerging diseases: a challenge to the horse industry.” American Horse Council, Wash-ington, DC. May 21

Edward Squires. Bioniche Animal Health, Animal Business meeting. Bogart, GA. Aug. 16-17

Edward Squires. World Federation of Sport Horses. Devon, PA. Sept. 29-30

Edward Squires. New Hampshire Veterinary Associa-tion. Manchester, NH. Nov. 3

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Edward Squires. Preservation of Equine Genetics Program, Colorado State University. Ft. Collins, CO. Nov. 30

Edward Squires. Bioniche Business Meeting. Balti-more, MD. Dec 4

Edward Squires. “Table Topics on Frozen Semen.” AAEP. Baltimore, MD. Dec 4-8

Peter Timoney. Invited presentation on contagious equine metritis, College of Veterinary Medicine, Okla-homa State University, Stillwater, OK. March 11-12

Peter Timoney. Attended the board of directors meet-ing of the National Institute for Animal Agriculture and gave a presentation at the equine committee meeting, Kansas City, MO. March 15-17

Peter Timoney. Horse Species Symposium “Pathogens and Reproductive Dysfunction in Horses,” The 2010 ADSA-PSA-AMPA-CSAS-WSASAS-ASAS Joint An-nual Meeting. Denver, CO. July 11-15

Peter Timoney. 114th Annual Meeting of the United States Animal Health Association. Minneapolis, MN.

Peter Timoney. 53rd Annual Conference of the Ameri-can Association of Veterinary Laboratory Diagnosti-cians. Minneapolis, MN.

Thomas Tobin. Oral presentation “Fatal Musculoskel-etal Injury Rates, Thresholds for Phenylbutazone and Pre-Race Examinations: A Preliminary Report,” Na-tional Horseman’s Benevolence and Protective Asso-ciation summer meeting. Minneapolis, MN. July 23

Mats Troedsson. “Current Topics on Broodmare Man-agement.” Conference for Veterinarians, Ohio State University, Columbus, Ohio.

Mats Troedsson. “Mechanisms of Sperm Transport and Elimination.” Ohio State University Short Course

speaker, Columbus, Ohio, Feb. 6-7

Mats Troedsson. Presented research on endometritis and sperm transport/elimination, panel discussion on CEM, Society for Theriogenology/American College of Theriogenologists. Seattle. Aug. 31- Sept. 5

Mats Troedsson. Presented a paper on benefits and potential consequences of the use antibiotics in se-men extenders, Minitube International Center for Biotechnology. Mt Horeb, WI. Nov. 2

Mats Troedsson. Table topic coordinator for CEM, American Association of Equine Practitioners. Balti-more, MD. Dec. 4-8

Velineni, S. and Timoney, J.F. “Expressive and se-creted proteins associated with capsule expression in Streptococcus zooepidenic from outbreaks of equine pneumonia. 110th annual meeting American Society of Microbiology. San Diego, CA, May

Willand, Z.A., Cook, S.J., Issel, C.J. and Cook, R.F. “Gag gene diversity in equine infectious anemia virus (EIAV) field isolates from the United States.” Presented at the 24th National Conference of Under-graduate Research (NCUR), Missoula, MT. April 16

Yeargan, M. and D.K. Howe. “Modification of enzyme-linked immunosorbent assays based on Sarcocystis neurona surface antigens (SnSAGs) for improved equine antibody detection.” Special Inter-est Group session on EPM, American College of Veterinary Internal Medicine (ACVIM), Anaheim, CA, June

Zhang, J., Go, Y. Y., Lu, Z., Meade, B. J., Timoney, P. J., and Balasuriya, U. B. R. “Development and characterization of an infectious cDNA clone of the modified live virus vaccine strain of equine arteri-tis virus and its potential as a vaccine vector.” 9th International Symposium on Positive Stranded RNA Viruses, Atlanta. May 17-21

STATEWIDENote: Many of the meetings held in Kentucky were international or national conferences or symposiums.

Ernie Bailey. “Equine Genetics and Applications to Veterinary Medicine.” Department of Veterinary Sci-ence Equine Diagnostic and Research Seminar Series. Lexington. March 25

Barry Ball. Session Chair for “Conception and Early Development.” 10th International Symposium on Equine Reproduction. Lexington. July 26-30

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DeNegri, R., Artiushin, S., Muthupalani, S., Fet-tinger, M., and Timoney, J.F. “Evaluation of re-combinant proteins of Streptococcus equi as vaccine components.” Kentucky Science and Engineering Foundation, Louisville.

Roberta Dwyer. “Farm Biosecurity,” Midwest Biosafety Network Biosafety Conference, Lexington. August

Roberta Dwyer. “Agrosecurity.” Department of Vet-erinary Science Seminar Series. Lexington. November

Roberta Dwyer. General equine anatomy; front limb anatomy; hind leg anatomy; specialized anatomy; dis-infecting equipment; disinfecting leather; and equine weight estimation. Presentations filmed for http://www.thehorse.com.

David Horohov. “Art and Science of Equine Vacci-nation,” Mid-America Veterinary Conference. Louis-ville. Oct. 11

Teri Lear. “Chromosome abnormalities in Horses” Kentucky Equine Management Internships (KEMI), Lexington. June

Teri Lear. “Repeated Early Embryonic Loss in Mares.” International Society of Equine Reproduc-tion. Workshop speaker, Lexington. July

James MacLeod. Cellular and molecular analyses of emergent tendon therapies. Kentucky Equine Veteri-nary Spring Seminar. Lexington.

James MacLeod. Equine Genome Project. The 2010 Thoroughbred Pedigree and Genetics Symposium, Lexington.

James MacLeod. Equine Genomics. Kentucky Quar-ter Horse Association Annual Convention, Lexington.

Karen McDowell. “The Role of Caterpillars in Mare Reproductive Loss Syndrome – An Environmen-tal Cause of Abortion.” Invited speaker at the 29th Annual Symposium for Reproductive Science and Women’s Health, Lexington. May 20-21

Karen McDowell. Participant at the International Symposium on Fungal Endophytes of Grasses, Lex-ington. June 28-July 1

Karen McDowell. Participant at the Symposium on Nutritional and Epigenetic Interactions. Lexington. July 1

Karen McDowell. Participant at the 10th International Symposium on Equine Reproduction, Lexington. July 26-30

Edward Squires. Presentation to the Pfizer Animal Health Technician Service Group, Gluck Equine Re-search Center, Lexington. Feb. 23

Edward Squires. International Chairman and Local Organizing Committee, 10th International Symposium on Equine Reproduction, Lexington. July 26-30

Edward Squires. Select Breeders Annual Meeting. Lexington. Oct. 11-12

Peter Timoney. Invited presentation at the 76th Annual Meeting of the American Mosquito Control Associa-tion, Lexington. March 29.

Peter Timoney. 10th International Symposium on Equine Reproduction, Lexington. July 26-30

Peter Timoney. 35th Annual Symposium of the Asso-ciation of Veterinary Microbiologists, Lexington. Aug. 4-7

Peter Timoney. 11th Annual Meeting of Select Breed-ing Services Network of Affiliated Laboratories, Lex-ington.

Thomas Tobin. “The Problem Of Dietary And Envi-ronmental Substances In Equine Forensic Science.” Oral presentation at the Kentucky Derby Conference. Louisville.

Thomas Tobin. Invited Speaker “Chemical Warfare,” The Sound Horse Conference. Louisville. Nov. 5-6

Mats Troedsson. Local Organizing Committee, 10th International Symposium on Equine Reproduction, Lexington. July 26-30

Mats Troedsson. “Pregnancy losses: Diagnosis and Management” and “Controlling CEM with antibiotic treated semen.” Kentucky Breeders’ Short Course, Fasig-Tipton, Lexington. January

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Gluck Equine Research Center

2010 Donors

Thank you to our 2010 donors who generously supported the Gluck Center’s

mission in improving the health and well-being of the horse.

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PLATINUMAlbert and Lorraine Clay

American Endowment FoundationBlood Horse PublicationsDarley at Jonabell Farm

Estate of Hilde R. ShapiroFlorida Horsemen’s Charitable FoundationGeoffrey C. Hughes Foundation Fellowship

Horseman’s Financial Group, Inc.Intervet

The Irwin Foundation Lloyd’s Underwriter Funds

The Lourie Foundation Mt. Brilliant Family Foundation

The R. D. & Joan Dale Hubbard FoundationRoemer Foundation

GOLDAshford, Inc.

Boehringer Ingelheim Vetmedica, Inc. Dr. Stuart E. Brown II

Centaur, Inc. Mr. John E. Craine

Denali StudMs. Adele B. Dilschneider

Fasig-Tipton Company, Inc.Dr. William D. Fishback Jr.

Hagyard-Davidson-McGee AssociatesThe Helen T. Andrews Foundation

Mrs. Louise Ireland HumphreyKentucky Thoroughbred Farm Managers’ ClubKentucky Thoroughbred Owners and Breeders

Milkhaus Veterinary Products, Inc. Dr. Janice Spencer MurrayMary K. Oxley Foundation

Dr. W. Thomas RiddleMr. Daniel M. Rosenberg

Misdee Wrigley and James Mather Miller Charitable Foundation


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BRONZE

Mr. Samuel A. BooneDr. Ruel Cowles

Edgewater Equine ClinicEquine Medical Associates

Dr. Luke H. FallonKentucky Trust Company

The Klein Family Foundation, Inc. Ms. Kristina G. Lu and Mr. Peter Morresey

Mr. and Mrs. Fred MitchellDr. Edward L. SquiresMr. Jack R. Swain IIIDr. Mats H. Troedsson

Miss Jane AtkinsonDr. and Mrs. William V. Bernard

Mrs. Melissa CantacuzeneDr. Thomas M. Chambers

Mr. Kyle W. CooperDr. Claire Latimer Embertson

Dr. Rolf M. EmbertsonMr. Mac Fehsenfeld

Hillside Stock Farm, LLC.Mrs. Martha K. Hoff

Mr. and Mrs. Steven A. HollandMr. Neil A. Howard

Mrs. Virginia HowardMr. Brent A. Jacobs

Dr. Robert A. LeonardMr. Tim McMurry

Mrs. Carol M. MillerMr. John P. MillerMrs. Judy P. Miller

Mrs. Betty W. MullenDr. Johnny M. SmithMr. Lloyd Schwartz

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Research Report 2010 - University of Kentucky

Documents

Transcript of Research Report 2010 - University of Kentucky