research in Glycoscience.€¦ · Sonia Serna, María Ercibengoa, Jose María Marimón, and...

2 Glycotechnology | Annual Report

The Glycotechnology Group at CIC biomaGUNE is engaged in both basic and applied research in Glycoscience.

Glycoscience is still a relatively understudied area but has a strong potential to provide groundbreaking solutions to major societal challenges in personalized medicine, pharmaceuticals, food and biomaterials.

In our research we focus specifically on Biomedical Glycoscience, investigating and exploiting structure and function of carbohydrates in the context of human biology and disease.

We do this by developing specific tools for glycan analysis and glycan-based intervention and by closely collaborating with a growing number of internationally recognized researchers.

The following pages illustrate the active major research lines in 2016 and our efforts in translating our results from basic science into commercially viable products.

Our main areas of research are:

• Carbohydrate Synthesis

• Glycan Microarrays

• Lectin Analysis

• Glycan Analysis

• Chemical Immunology

3

Dr. Sonia Serna Postdoctoral Researcher

Raquel Pazos Research Technician

Elisabete Elosegui Research Technician

Begoña Echeverría Research Technician

Julie Pham IMMUNOSHAPE

PhD Student (until May 2018)

Dr. Niels-Christian Reichardt Principal Investigator

Dr. Manuel Martín Lomas CIC biomaGUNE Founding Director

Anna Cioce IMMUNOSHAPE

PhD Student (until Jun. 2018)

Bárbara Kuhn PhD Student

(until Nov. 2018)

Dr. Cristina Díez IMMUNOSHAPE Project Manager

Glycotechnology | Annual Report

Dr. Nerea Ruiz Postdoctoral Researcher

(until Nov. 2018)

María Alcalá Research Technician

Dr. Raik Archtswager Postdoctoral Researcher

María Gómez Industrial PhD Student with Asparia Glycomics

Charles Williams Joint PhD Student with CIC bioGUNE (since May 2018)

Anna Ballesteros Industrial PhD Student with Asparia Glycomics

(since Dec. 2018)


Mass spectrometry for glycan biomarker discovery

O. Aizpurua-Olaizola, J. Sastre Toraño, J.M. Falcon-Prez, C. Williams, N. Reichardt, G.-J. Boons

TrAC Trends in Analytical Chemistry 2018, 100, pp 7–14

DOI: 10.1016/j.trac.2017.12.015

The association between aberrant glycosylation of proteins and many cancers, and autoimmune and inflammatory diseases has been known for many years. Altered glycosylation can occur at the onset and during disease progression and identifying these changes at an early stage may greatly increase survival and improve quality of life. However, the identification of these biomarkers has not been easy, mainly due to the structural diversity and numerous possible glycan isomers. Fortunately, glycomics is becoming more feasible due to major improvements in mass spectrometry and separation science. The present review discusses recent methods for mass-spectrometry (MS) based glycomics for the identification of glycan biomarkers. Recent MS techniques with and without coupling to liquid chromatography, capillary electrophoresis or ion mobility spectrometry are described, and the most recent glycan biomarker studies are presented and future prospects discussed.


Review Article: Glycosylation of extracellular vesicles: current knowledge, tools and clinical perspectives

Charles Williams, Felix Royo, Oier Aizpurua-Olaizola, Raquel Pazos, Geert-Jan Boons, Niels-Christian Reichardt, Juan M. Falcón-Pérez

Journal of Extracellular Vesicles 2018, 7:1, 1442985

DOI: 10.1080/20013078.2018.1442985

It is now acknowledged that extracellular vesicles (EVs) are important effectors in a vast number of biological processes through intercellular transfer of biomolecules. Increasing research efforts in the EV field have yielded an appreciation for the potential role of glycans in EV function. Indeed, recent reports show that the presence of glycoconjugates is involved in EV biogenesis, in cellular recognition and in the efficient uptake of EVs by recipient cells. It is clear that a full understanding of EV biology will require researchers to focus also on EV glycosylation through glycomics approaches. This review outlines the major glycomics techniques that have been applied to Evs in the context of the recent findings. Beyond understanding the mechanisms by which EVs mediate their physiological functions, glycosylation also provides opportunities by which to engineer EVs for therapeutic and diagnostic purposes. Studies characterising the glycan composition of EVs have highlighted glycome changes in various disease states, thus indicating potential for EV glycans as diagnostic markers. Meanwhile, glycans have been targeted as molecular handles for affinity-based isolation in both research and clinical contexts. An overview of current strategies to exploit EV glycosylation and a discussion of the implications of recent findings for the burgeoning EV industry follows the below review of glycomics and its application to EV biology.


On-chip screening of a glycomimetic library with C-type lectins reveals structural features responsible for preferential binding of dectin-2 over DC-SIGN/R and langerin

Medve, L; Achilli, S; Serna, S; Zuccotto, F; Varga, N; Thépaut, M; Civera, M; Vivès, C; Fieschi, F; Reichardt, N; Bernardi, A.

Chemistry - A European Journal, 2018

DOI: 10.1002/chem.201802577

A library of mannose- and fucose-based glycomimetics was synthesized and screened in a microarray format against a set of C-type lectin receptors (CLRs) that included DC-SIGN, DCSIGNR, langerin and dectin-2. Glycomimetic ligands able to interact with dectin-2 were identified for the first time. Comparative analysis of binding profiles allowed to probe their selectivity against other CLRs.

Glycan Microarray-Assisted Identification of IgG Subclass Targets in Schistosomiasis

Y. Y. Michelle Yang, Angela van Diepen, Katarzyna Brzezicka, Niels-Christian Reichardt and Cornelis H. Hokke

Frontiers in Immunology, 09 October 2018, Volume 9, Article 2331

DOI: 10.3389/fimmu.2018.02331 Infection with schistosomes is accompanied by the induction of antibodies against the parasite. Despite having IgG against both protein and glycan antigens, infected individuals remain chronically infected until treated, and re-infection is common in endemic areas as immunity does not develop effectively. Parasite specific IgG subclasses may differ in functionality and effectivity with respect to effector functions that contribute to parasite killing and immunity. In this study, we investigated if specific IgG subclasses target specific antigenic schistosome glycan motifs during human infection. Sera from 41 S. mansoni infected individuals from an endemic area in Uganda were incubated on two glycan microarrays, one consisting of a large repertoire of schistosome glycoprotein- and glycolipid- derived glycans and the other consisting of chemically synthesized core xylosylated and fucosylated N-glycans also expressed by schistosomes. Our results show that highly antigenic glycan motifs, such as multi-fucosylated terminal GalNAc(b1-4)GlcNAc (LDN) can be recognized by all IgG subclasses of infection sera, however with highly variable intensities. Detailed examination of core-modified N-glycan targets revealed individual antibody responses specific for core-xylosylated and core a3-fucosylated glycan motifs that are life stage specifically expressed by schistosomes. IgG1 and IgG3 were detected against a range of N-glycan core structures, but IgG2 and IgG4, when present, were specific for the core a3-fucose and xylose motifs that were previously found to be IgE targets in schistosomiasis, and in allergies. This study is the first to address IgG subclass responses to defined helminth glycans.


Chemoenzymatic Synthesis of N-glycan Positional Isomers and Evidence for Branch Selective Binding by Monoclonal Antibodies and Human C-type Lectin Receptors

Begoña Echeverria, Sonia Serna, Silvia Achilli, Corinne Vivès, Julie Pham, Michel Thépaut, Cornelis H. Hokke, Franck Fieschi, Niels-Christian Reichardt

ACS Chemical Biology 2018

DOI: 10.1021/acschembio.8b00431

Here, we describe a strategy for the rapid preparation of pure positional isomers of complex N-glycans to complement an existing array comprising a larger number of N- glycans and smaller glycan structures. The expanded array was then employed to study context-dependent binding of structural glycan fragments by monoclonal antibodies and C-type lectins. A partial enzymatic elongation of semiprotected core structures was combined with the protecting-group-aided separation of positional isomers by preparative HPLC. This methodology, which avoids the laborious chemical differentiation of antennae, was employed for the preparation of eight biantennary N-glycans with Galβ1,4GlcNAc (LN), GalNAcβ1,4GlcNAc (LDN), and GalNAcβ1,4-[Fucα1,3]GlcNAc (LDNF) motifs presented on either one or both antennae. Screening of the binding specificities of three anti-LeX monoclonal IgM antibodies raised against S. Mansoni glycans and three C-type lectin receptors of the innate immune system, namely DC-SIGN, DC-SIGNR, and LSECtin, revealed a surprising context-dependent fine specificity for the recognition of the glycan motifs. Moreover, we observed a triking selection of one individual positional isomer over the other by the C-type lectins tested, underscoring the biological relevance of the structural context of glycan elements in molecular recognition.


Measuring Bacterial Glycosyl Hydrolase Activity with a Soluble Capture Probe by Mass Spectrometry

Sonia Serna, María Ercibengoa, Jose María Marimon, and Niels-Christian Reichardt

Analytical Chemistry, 2018, Article ASAP

DOI: 10.1021/acs.analchem.8b02434

A solution-phase enzymatic assay has been developed to track bacterial glycosyl hydrolase activity by surface-assisted MALDI-TOF mass spectrometry. Lactose was equipped with an azide-functionalized linker and was supplemented to bacterial cultures as an artificial substrate for bacterial β-galactosidase enzyme. The azide linked glycoside probe was then covalently captured on an alkynefunctionalized indium tin oxide sample plate via a bioorthogonal copper-catalyzed azide alkyne cycloaddition (CuAAC). The noncovalent immobilization of the alkyne capture tag via hydrophobic interactions on the ITO-sample plate allowed the analysis of the probe conjugate by surface-based mass spectrometry. The ratio of digested to nondigested lactose probe was then employed as a measure for bacterial hydrolase activity, which correlated well with bacterial growth measured by optical density. In addition, we established in a proof of concept experiment that the setup was well suited to identify antibiotic susceptibility of bacterial strains with a performance comparable to current state-of-the-art methods. While the proof of concept version is limited to the identification of a single enzyme activity, we envisage that the use of multiple substrate probes in a multiplexed version will allow the quantification of various glycosyl hydrolase activities with clinical relevance in a single experiment.


Fluorescent Neoglycoprotein Gold Nanoclusters: Synthesis and Applications in Plant Lectin Sensing and Cell Imaging Katarzyna Alicja Brzezicka, Sonia Serna, Niels-Christian Reichardt

Nanoscale Research Letters, 2018, 13:360

DOI: 10.1186/s11671-018-2772-2

Carbohydrate-protein interactions mediate fundamental biological processes, such as fertilization, cell signaling, or host-pathogen communication. However, because of the enormous complexity of glycan recognition events, new tools enabling their analysis or applications emerge in recent years. Here, we describe the first preparation of neoglycoprotein functionalized fluorescent gold nanoclusters, containing a biantennary N-glycan G0 as targeting molecule, ovalbumin as carrier/model antigen, and a fluorescent gold core as imaging probe (G0-OVA-AuNCs). Subsequently, we demonstrate the utility of generated G0-OVA-AuNCs for specific sensing of plant lectins and in vitro imaging of dendritic cells.

Letter to the editor: Identification of dominant anti-glycan IgE responses in school-children by glycan microarray

Abena S. Amoah, Elias K. Asuming-Brempong, Benedicta B. Obeng, Serge A. Versteeg, Irene A. Larbi, Yvonne Aryeetey, Thomas A.E. Platts-Mills, Adriano Mari, Katarzyna Brzezicka, Ben A. Gyan, Mohamed Mutocheluh, Daniel A. Boakye, Niels-Christian Reichardt, Ronald van Ree, Cornelis H. Hokke, Angela van Diepen, Maria Yazdanbakhsh

J. Allergy Clin. Immunol. 2018 | DOI: 10.1016/j.jaci.2017.09.040


Glycan recognition: Glycomics & Glycoarrays (Invited)

Niels Reichardt

GLYCOVAX 4th Training Event, CIC bioGUNE, Derio (Spain)

12 March 2018

Chemo-enzymatic On-chip Synthesis of N-glycan mimetics

Anna Cioce, Niels Reichardt

IMMUNOSHAPE Int. Symposium on Glycoimmunology, San Sebastian (Spain)

11-13 July 2018

Synthesis of S. mansoni inspired O-glycans for improved CLR targeting

Julie Pham, Anna Cioce, Alvaro Hernandez, Niels C. Reichardt


11-13 July 2018

Exploring the role of exosome glycosylation in cellular uptake

Charles Williams, Raquel Pazos, Felix Royo, Niels Reichardt, Juan M Falcon-Perez


11-13 July 2018

IgG Glycosylation as Potential Disease Marker for Thyroid Cancer. Development of a Serum Sample Preparation Protocol for Absolute Glycan Analysis and Proof of Concept Study Nerea Ruiz, Elisabete Elosegui, Eugènia Mato, Rosa Corcoy, Niels C. Reichardt IMMUNOSHAPE Int. Symposium on Glycoimmunology, San Sebastian (Spain) 11-13 July 2018

A C-type lectin receptor (CLR)-Fc fusion protein library as a toolbox to explore the intricate interplay of CLR-pathogen interactions

João T. Monteiro, Silvia Achilli, Sabine Mayer, Kathleen Schön, Sonia Serna, Niels Reichardt, Stefanie C. Becker, Corinne Vivès, Franck Fieschi, Bernd Lepenies


11-13 July 2018

Microarray-based screening of C-type lectins against a glycomimetics library

Laura Medve, Silvia Achilli, Sonia Serna, Fabio Zuccotto, Monica Civera, Corinne Vivès, Franck Fieschi, Niels C. Reichardt, Anna Bernardi


11-13 July 2018

Synthesis and application of dendritic carbohydrate multivalent systems to study recognition events by lectins

Antonio Di Maio, Blanka Didak, Anna Cioce, Silvia Achilli, Corinne Vivès, Michel Thépaut, Franck Fieschi, Niels C. Reichardt, Ludovic Landemarre, Javier Rojo


11-13 July 2018


Development of Glycomimetics for Dendritic cell Targeting (Invited)

Katarzyna Brezicka, Begoña Echevarria, Sonia Serna, Barbara Kuhn, Joao Monteiro, Silvia Achilli, Franck Fieschi, Bernd Lepenies, Niels-Christian Reichardt

29th International Carbohydrate Symposium – ICS’2018, Lisbon (Portugal)

14-19 July 2018

Insights and lessons from microarray experiments on the glycan binding specificity of immune receptors (Invited)

Niels Reichardt

29th Joint Glycobiology Conference, Ghent (Belgium)

21-23 October 2018

Mannitou antibody as a potential diagnostic and therapeutic tool

Stefania Robakiewicz, Sonia Serna, Begoña Echeverria, Diego Charro, Nicola Abrescia, Ana Arda, Jesús Jiménez-Barbero, Niels Reichardt, Julie Bouckaert


11-13 July 2018

DC-SIGN, DC-SIGNR and LSECtin: potential selective interaction with positional antennae isomers

Silvia Achilli, Begoña Echeverria, Sonia Serna, Corinne Vivès, Julie Pham, Michel Thépaut, Cornelis Hokke, Franck Fieschi, Niels-Christian Reichardt


14-19 July 2018

C-type lectin targeting with glycomimetics: a screening in microarray-format

Laura Medve, Silvia Achilli, Sonia Serna, Fabio Zuccotto, Monica Civera, Corinne Deniaud, Franck Fieschi, Niels-Christian Reichardt, Anna Bernardi


14-19 July 2018

Insights and lessons from microarray experiments on the glycan binding specificity of immune receptors (Invited)

Niels Reichardt

Jornada del Grupo de Hidratos de Carbono RSEQ, Sevilla (Spain)

26 November 2018

Microarrays for Functional Glycomics (Invited) Niels Reichardt BIFI, Zaragoza (Spain) 16 November 2018


On-chip synthesis of N-glycan mimetics


16th Iberian Peptide Meeting (16EPI) / 4th Chemical Biology Group Meeting (4GEQB), Barcelona (Spain)

5-7 February 2018

Chemo-enzymatic On-chip Synthesis of N-glycan mimetics



11-13 July 2018

Synthesis of S. mansoni inspired O-glycans for improved CLR targeting

Julie Pham, Anna Cioce, Alvaro Hernandez, Niels C. Reichardt


11-13 July 2018

Measuring bacterial glycosyl hydrolase activity with a soluble capture-probe by mass spectrometry

Sonia Serna, María Ercibengoa, Jose María Marimón, Niels-Christian Reichardt


11-13 July 2018




11-13 July 2018

Microarray-based screening of C-type lectins against a glycomimetics library

Laura Medve, Silvia Achilli, Sonia Serna, Fabio Zuccotto, Monica Civera, Corinne Vivès, Franck Fieschi, Niels C. Reichardt, Anna Bernardi


11-13 July 2018





14-19 July 2018

Measuring bacterial glycosyl hydrolase activity with a soluble capture-probe by mass spectrometry

Sonia Serna, María Ercibengoa, Jose María Marimón, Niels-Christian Reichardt


14-19 July 2018

Advanced tools for glycans absolute quantification

Pilar Castellnou, Juan Echevarria, Alvaro Hernández, Javier Calvo, Nerea Ruiz, Niels Reichardt


11-13 July 2018


Kick-off meeting Elkartek 2018

CIC biomaGUNE, San Sebastian (Spain)

18 April 2018

Niels Reichardt, as leader of the Elkartek 2018 project, organized a kick-off meeting for the Elkartek partners together with his group at CIC biomaGUNE. After a first introductory talk of Niels Reichartd about the Elkartek project (objectives, research plan, partners, expected results and technology transfer) and his group, a representative of each partner presented their group expertise and their contributions to the common project: Nerea Briz (Tecnalia), Marco Marradi (Cidetec), Luis Kabongo (Vicomtech), Joseba Garrido (Neiker), Felipe Goñi (Gaiker), José María Marimón (Biodonostia Microbiology) and Charles Lawrie (Biodonostia Oncology).


IMMUNOSHAPE International Symposium on Glycoimmunology

Understanding and Shaping glycan mediated immune responses

Auditorium Technological Park Miramón, San Sebastian (Spain)

11-13 July 2018

This 2.5 day symposium provided a forum for scientists from chemistry, immunology and glycobiology to discuss recent advances in our understanding and the opportunities for shaping glycan mediated immune responses.

Keynote lectures from world leaders addressing major topics in glycoimmunology provided the framework for a large number of short talks and flash presentations together with a poster session showcasing recent achievements from major European research groups active in the field


Kick-off meeting Red de Excelencia Temática GLYCOBIOCHEM

CIC bioGUNE Derio (Spain)

7 September 2018

Niels Reichardt, as coordinator of the GLYCOBIOTECH network funded by the Spanish government, organized a kick-off meeting for network partners at CIC bioGUNE. After a first introductory talk of Niels Reichartd about the network, a representative of each partner and some local scientists presented their group expertise and their contributions to the common project: Jesús Jiménez-Barbero (CIC bioGUNE), Marcelo Guerin (CIC bioGUNE), Alberto Fernández-Tejada (CIC bioGUNE), Francisco Corzana (University of La Rioja), Antoni Planas (IQS), Pedro Merino (University of Zaragoza), Ramón Hurtado (University of Zaragoza/BIFI), Juan Luis Asensio (CSIC-IQOG), Sergio Castillón (University Rovira i Virgili), Pedro Nieto (CSIC-IIQ), and Javier Cañada (CSIC/CIB).

Niels Reichardt

Basque Government (Vitoria/Gasteiz)

21 November 2018

The Basque Government recognizes the work carried out in research by Niels Reichardt, leader of the Glicotechnology laboratory of CIC biomaGUNE and co-founder of the spin-off Asparia Glycomics.


Julie Pham

Synthesis, screening and use of parasite O-glycans and mimetics for improved CLR targeting

Directors: Niels-Christian Reichardt, Jesús Jiménez-Barbero University of Basque Country (UPV), San Sebastian / CIC biomaGUNE

21 November 2018

N.C. Reichardt:

Agencia Nacional de Evaluación y Prospectiva (ANEP)

European Commission Horizon 2020

N.C. Reichardt:

Since 2017: Member of editorial board Carbohydrate Research

Peer reviewer for Nature, Angewandte Chemie, JACS, Anal Chem, Carb Res., Chemistry, Accounts of Chemical Research

Spokeperson of the Carbohydrate Group of the Spanish Royal Society of Chemistry (CH-RSEQ) since 26.11.2018


Ernesto Rodríguez Camejo

Synthesize the Core 2 O-glycans with the Lewis x antigen to study if these glycans can modulate Dendritic Cells / Macrophages

Home Institution: VU medisch centrum (VUmc), Amsterdam (The Netherlands)

January 2018

Valentin Cognet

Development a chip-based method to detect protein-carbohydrate interactions based on the energy transfer between a fluorophore and gold glyconanoparticles

Home Institution: Midatech Pharma, Bilbao (Spain)

April 2018

Antonio Di Maio

Experiments of stability of novel dendrimeric structures for the generation of interesting tools to investigate Lectin-Carbohydrate interactions

Home Institution: IIQ-CSIC Seville (Spain)

May 2018


Rafael Prados

Harnessing the Mycobacterium tuberculosis cell surface’s physiology to generate better anti‐infective tools

“Ramon y Cajal” Investigator – CIC bioGUNE, Derio (Spain)

17 January 2018

Rob Field

Sugars, Natural Products and Algal Blooms

John Innes Center (UK)

22 March 2018

Catherine Thornton

Differences in post-T cell receptor metabolic response of naive versus memory human CD4+ T cells

Swansea University (UK)

1 June 2018

Celso Reis

Altered glycosylation in Cancer – targeting tumor heterogeneity and therapeutic implications

IPATIMUP/I3S/University of Porto (Portugal)

12 December 2018

20

Our multidisciplinary research is only possible in collaboration with a growing

number of international recognized researchers. Many current collaborations are

managed within the IMMUNOSHAPE project that runs until 2018.

• Prof. Bernd Lepenies, Infection Immunology. University of Veterinary Medicine

Hannover. Research Center for Emerging Infections and Zoonoses (RIZ), Germany

• Anna Bernardi, Chemistry Department. Università degli Studi di Milano, Italy

• Sabine Flitsch, Manchester Institute of Biotechnology (MIB). The University of

Manchester, UK

Other current collaborations include:

• Dr. Ron Hokke, Parasite Glycobiology Group. Department of Parasitology. University

Medical Center Leiden, Netherlands

• Prof. Jesús Jiménez-Barbero, Chemical Glycobiology Lab. CIC bioGUNE, Bilbao, Spain

• Dr. Jesús Angulo, School of Pharmacy. University of East Anglia, Norwich, UK

• Prof. Juan Falcón, Exosomes Lab. CIC bioGUNE , Bilbao, Spain

• Dr. Alberto de Leiva, Departament of Medicine and Director of Department of

Endocrinology and Nutrition. Hospital San Pau, Barcelona, Spain

• Dr. Emilio Pérez-Trallero, University Hospital Donostia, Spain

• Dr. Dolores Gonzales, Departament of Biochemistry and Molecular Pharmacology.

Instituto de Parasitología y Biomedicina-Lopez Neyra, Granada, Spain

• Dr. Héctor Peinado, Microenvironment and Metastasis Group. CNIO, Madrid, Spain

• Dr. Katie Doores, Infectious Diseases. Faculty of Life Sciences and Medicine. Kings

College London, UK

• Prof. Manfred Wuhrer, Center for Proteomics and Metabolomics. Leiden University

Medical Center, Netherlands

• Prof. Maurizio Prato, Carbon Bionanotechnology Group at CIC biomaGUNE and

Università degli Studi di Trieste, Italy

Glycotechnology | Annual Report


GLYCOIMMUNOTECH (Retos)

Grant No. CTQ2014-58779-R

Tools to study and exploit the role of glycans in adaptive and innate immunity

2015-2018

Elkartek (Coordination)

KK-2018/00038

Herramientas y oportunidades para la glicociencia médica

2018-2019

GLYCOBIOMED (Retos)

Grant No. CTQ2017-90039-R

Novel tools and Approximations for Biomedical Glycoscience

2018-2020

GLYCOBIOCHEM

Grant No. CTQ2017-90719-REDT

Red Española Temática de Excelencia de Glicobiología Química

2018-2019

GlycoNat (Bikaintek - Industrial Doctorate) 20-AF-W2-2018-00005

Desarrollo de metodología para el aislamiento de N-glicanos de fuentes naturales

2018-2021

Industrial Doctorate (with Asparia Glycomics)

Grant No. DI-16-08914

Síntesis quimienzimática de glicanos y glicopéptidos marcados isotópicamente y su aplicación como estándares internos en el glicoanálisis por espectrometría

2018-2020

Retos Colaboración (with Asparia Glycomics)

Grant No. RTC‐2017‐6126‐1

Herramientas Avanzadas para la Glicómica Cuantitativa y Funcional

2018-2021


Glyco Thyroid Cancer

No. CIB16-NM012

Intramural project

European Commission

IMMUNOSHAPE (Coordination)

H2020-MSCA-ITN-2014-ETN-642870

Development of Selective Carbohydrate Immunomodulators Targeting C-type Lectin Receptors on Antigen Presenting Cells

2015-2018

www.immunoshape.eu

GlycoTB - Torres Quevedo (with Asparia Glycomics)

Grant No. PTQ-17-09070

Desarrollo de un método de glico-diagnóstico clínico basado en el análisis de anticuerpos de suero. Optimización del método de análisis y aplicación en el diagnóstico de tuberculosis latente

2019-2020

Juan de la Cierva Incorporación

Grant No. ICIJ-2017-33304

Blocking of CLR tumour immune suppresion

2019-2021

http://www.immunoshape.eu/

research in Glycoscience.€¦ · Sonia Serna, María Ercibengoa, Jose María Marimón, and...

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