Crayfish News Volume 35 Issue 3-4: Page 1

ISSN: 1023-8174 (print), 2150-9239 (online)

The Official Newsletter of the International Association of Astacology

December 2013

Volume 35, Issue 3-4

Cover Story 1

President’s Corner 2

IAA Related News 3

Short Articles 5

Crayfish Workshop:

Status Quo of the

Crayfish Astacus astacus

in Lower Saxony and

Bremen, Germany

5

Australian Endangered Species: Victorian

burrowing crayfish

6

Crayfish Plague Agent Detected in the ‘New’

Invasive North American Crayfish Orconectes

immunis in Germany

8

Literature of Interest to Astacologists

14

Inside this issue:

Photo of the Istrian Peninsula region, which was visited during the meeting excursion.

T he Regional European Crayfish Meeting (CrayCro) was held from 26th till 28th of

September 2013 in Rovinj, Croatia. This inter-national meeting, organised by the Croatian Biological Society, gathered 58 scientists from 18 European countries, including, for the first time, participants from Slovenia, Serbia and Turkey.

The meeting was opened by Ivana Maguire, in the name of the organising com-mittee, and Goran Klobučar, in the name of the Croatian Biological Society. After the opening ceremony, Leopold Füreder wel-comed participants in the name of the IAA with a short overview of the IAA annual activ-ities. His speech was followed by an overview of freshwater crayfish research in Croatia by Ivana Maguire. During the meeting, a total of 31 oral presentations were given and 30

poster presentations displayed. Oral presen-tations were organised through 6 sessions: 1) Biogeography, 2) Crayfish Genetics and Phy-logeny, 3) Behaviour, Ecology and Biology, 4) Diseases, Pathogens and Epibionts, 5) Aqua-culture and Fisheries, and 6) Conservation and Management, while the poster session was traditionally accompanied with a biodi-versity of tastes. The session on Behaviour, ecology and biology was dedicated to our late colleague, Francesa Gherardi, and prior to the session, Catherine Souty-Grosset gave a moving Francesa Gherardi memorial lec-ture. During the meeting, members of the Scientific Committee (Lennart Edsman, Leo-pold Füreder, Frederic Grandjean, Sandra Hudina, Japo Jussila, Goran Klobučar, Ivana Maguire and Catherine Souty-Grosset) had a difficult task to evaluate and rank the best

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Regional European Crayfish Meeting – CrayCro

Crayfish News Volume 35 Issue 3-4: Page 2

The International Association of Astacology (IAA), founded in Hintertal, Austria in 1972, is dedicated to the study, conservation, and wise utilization of freshwater crayfish. Any individual or institution interested in furthering the study of astacology is eligible for membership. Service to members includes a quarterly newsletter (Crayfish News), a membership directory, biennial international symposia and publication of the journal Freshwater Crayfish.

Secretariat:

The International Association of Astacology has a permanent secretariat managed by James Stoeckel. Address: IAA Secretariat, Room 203, Swingle Hall, Department of Fisheries and Allied Aquacultures, Auburn University, AL 36849-5419, USA.

Tel: +1(334) 844-9249 / Fax: +1(334) 844-9208 E-mail: jimstoeckel@auburn.edu

Web page: http://iz.carnegiemnh.org/crayfish/IAA/ Webmaster: James W. Fetzner Jr.

E-mail: FetznerJ@CarnegieMNH.Org

IAA Executive Board Members:

In addition to the IAA Officers and Past President, the board includes Jason Coughran (Australia), Antonio Garza de Yta (México), Tadashi Kawai (Japan), Ivana Maguire (Croatia), Steph Parkyn (Australia), Alastair Richardson (Tasmania) and Chris Taylor (USA).

Officers:

Leopold Füreder, President — Institute of Ecology, University of Innsbruck, Technikerstr. 25, A-6020 Innsbruck, Austria. E-mail: leopold.fuereder@uibk.ac.at Susan B. Adams, President-Elect — USDA Forest Service, 1000 Front Street, Oxford, MS, 38655, United States of America. E-mail: sbadams29@gmail.com Lennart Edsman, Secretary — Swedish University of Agricultural Sciences, SLU Aqua, Institute of Freshwater Research, Stangholmsvagen 2, Drottningholm, Sweden, SE-178 93. E-mail: lennart.edsman@slu.se James W. Fetzner Jr., Immediate Past President — Section of Invertebrate Zoology, Carnegie Museum of Natural History, 4400 Forbes Avenue, Pittsburgh, PA 15213-4080. United States of America. E-mail: FetznerJ@CarnegieMNH.Org

Statements and opinions expressed in Crayfish News are

not necessarily those of the International Association of Astacology.

This issue edited by James W. Fetzner Jr.

President’s Corner

Leopold Füreder, Ph.D.

IAA President (Austria)

Dear IAA Members:

Rather quickly, another year has almost passed, leaving us with a cocktail of good and sad ingredients. Several great activities included the IAA being actively involved in various parts of the world. However, major losses were also experienced along the way. We are still grieving for our colleague and friend Francesca Gherardi (1955-2013), who lost her fight against the tricky disease. She was a long-time member of the IAA, served in several board positions and was IAA-President from 2004 to 2006. Beyond her active time as a board member, she assisted in several thematic networks for crustacean research and conservation. Apart from being a great scientist and mentor to many colleagues and students, she was a lovely and charming friend to many colleagues in Europe and around the world. We miss her a lot – both as a highly acknowledged astacologist, and a gracious mentor and friend!

Just a few weeks ago, we had to hear about another loss of a great astacologist from the very beginning of the IAA: Per Brinck (1919-2013) was the first President of the IAA from 1972 – 1974. He took part in most of the early symposia and was one of the pioneers in ecology. Indeed, Francesca and Per are and will be greatly missed!

During the past year, several interesting scientific events were organised with the help of the IAA or where we successfully participated. A special session “Recent advances in crayfish biology, ecology, and conservation”, organized by Brian Helms at the annual meeting of the Society of Freshwater Science, held in Louisville, Kentucky, May 20 – 24, 2012, resulted in a special section within the Journal of Freshwater Science.

A selection of 12 papers are now available on the webpage of Freshwater Science. The IAA helped to support this special session.

In San José, Costa Rica, The Crustacean Society (TCS) and the Latin American Association of Carcinology held their summer meeting from July 7 – 22, 2013. Tadashi Kawai, as the IAA liaison officer, intensively discussed the upcoming IAA20 in Sapporo, Japan, and agreed upon a common session with TCS. One day will be dedicated to Francesca Gherardi. Tadashi Kawai also garnered a TCS student sponsorship for attending the joint IAA-TCS meeting next September in Sapporo, Japan.

Another key conference on crayfish research was the CRAYCRO meeting in Rovinj, back in September 2013. The IAA board member Ivana Maguire and Goran Klobučar (Croatian Biological Society) and their colleagues organised a great “European IAA-regional meeting”. The CRAYCRO meeting stands in a long line of outstanding events and activities of European meetings, usually held every other year in between the regular IAA meetings. We already had several of this kind in Poitiers, organised by Catherine Souty-Grosset and colleagues. She and her colleagues together with specific follow-up activities established a very sound basis for European crayfish research. These activities became a nucleus for several research directions. Today in Europe, we now know a lot about the biogeography and ecology of large freshwater invertebrates and the nature and functioning of a deadly crayfish disease, perhaps more so than any other region worldwide.

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Crayfish News Volume 35 Issue 3-4: Page 3

IN MEMORIAM

Per Brinck (1919-2013)

Per Brinck was born September 4th, 1919 in Helsingborg and passed away on October 6th, 2013 at the age of 94. Per was an ambitious student – at the age of 19 he had already produced his first scientific paper. He studied veterinary medicine and worked several years as a veterinarian (during WWII he served in the Swedish Defence Forces, working mainly with horses!).

Thereafter, he took up science and field-work at the University of Lund, and he completed his Ph.D. thesis on Plectopera (stoneflies). Later, he turned his main interest to the Gyrinidae (whirligig beetles), where he soon became a world authority.

From 1950 – 1951, Per was the leader of an expedition to South Africa which resulted in the compilation of the “South African Animal Life” series, a 15 volume set on the region’s biodiversity. This also resulted in his life-long love for Africa. Per returned to that continent as often as he could, but he also undertook expeditions to other parts of the world. He often found himself in war-zones, revolutions, and other hostilities, but Per had a fantastic talent for talking himself out of trouble. He met every person with respect and humor and made friends everywhere.

Per Brinck was appointed a Professor of Zoology at Lund in 1958. He saw a great need for ecological research in Scandinavia and he successfully started working to improve it. One of his first steps was to vitalize the hibernating magazine “Oikos”.

Per had very strong expectations of his students, but his

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IAA Related News When I started this period of being President, I envisioned

that there were potential new avenues for crayfish research, including their sustainable management and conservation in two parts of the world: Central and South America and Eastern European countries. While Central and South America is still on the list and might be intensified after the next IAA meeting in Japan, crayfish research in the Eastern European countries is more active than ever. Most of these countries have well trained and enthusiastic scientists, however, most of them lack access to national and international research networks and funding sources. The actual financial crisis in the EU has often been used as killing arguments for no support. At various locations, I met disappointed scientists from universities, academies and museums complaining about a disproportional growth of administration and controlling bodies diminishing even further the support of science. Internationally well acknowledged scientists and experts are caught in their non-supportive environments. Although we might only achieve minor change in the science politics and funding schemes, we may produce a successful environment for crayfish research and collaboration with the new European countries. With only a little investment we can continue to widen our network, by inviting scientists, initiating collaborative research and workshops. The costs are actually not very high and it would open new research areas and perspectives. The historical issues of the Danube countries and the Balkan still have their consequences for the modern biogeography of certain species, including freshwater crayfish. The study of the distribution of European crayfish, the bio-complexity of all the still existing and — compared to Central Europe — less degraded habitats could lead to new insights and understanding. Just recently, several papers on crayfish from the new European countries were published in leading freshwater journals. Actually, my students and I have fruitful collaborations with Croatian, Bulgarian and Romanian scientists. We use freshwater crayfish as a model for research, but also for a sustainable socio-scientific interaction.

Next year, we plan to move even further East (excuse me – only the European view – it may certainly be different for colleagues from the US, Canada and Australia). Tadashi Kawai and several IAA board members are already preparing for a large conference, breaking ground in East Asia. Well discussed already in San José, the joint conference with the TCS will certainly be a success.

In this respect we can look forward to a fruitful new year in 2014! But now we should lean back, relax and enjoy this issue of Crayfish News. Again we are very grateful to Jim Fetzner whose continuing effort makes this possible. With the assistance of Abby Stancliffe-Vaughan, he worked hard to follow up with the last two years of issues. Thanks to all of you!

We are now looking forward to the Christmas and New Year break and the opportunity to wind down and relax. I wish you all the very best for Christmas and a prosperous and happy New

Year in 2014! Keep in touch! H

Sincerely,

Leopold Füreder

IAA President

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Crayfish News Volume 35 Issue 3-4: Page 4

students oral presentation. In a close competition, the prizes for the best oral presentations went to Jiří Svoboda, Daniela Ghia and Christian Berger. The student posters were evalu-ated by Stephanie Peay, Eva Balcarová, Christoph Chucholl, Christian Berger and Sandra Hudina, and the prizes for the best posters went to Agata Mrugala, Buket Yazicioglu, Fran Rebrina and Katrin Kaldre.

Throughout the meeting, many lively scientific discus-sions were held and the latest crayfish news was exchanged, as well as new collaborations arranged. One of the conclu-sions of the meeting was the need to organise European crayfish meetings on a more regular basis, every two years (alternating with IAA symposia), and Anne Schrimpf pro-posed to host the next one in Landau (Germany) in 2015; this idea was widely accepted and supported.

The journal Knowledge and Management of Aquatic Ecosystems kindly offered the opportunity of a special issue about the most interesting results presented during CrayCro.

But the meeting wasn’t all about work; there was also enough time for enjoying the local Croatian gastronomy and beverages.

After the official Meeting closure, a one day excursion was organised around the interior of the Istrian Peninsula in order to visit some of the crayfish sites, as well as some of the old medieval towns. The original plan had to be changed due to weather conditions. For sure, this excursion will be remembered by all due to the rainy weather and the peculi-ar touristic guide, Maria, who unknowingly coined the term ‘we are orconected’.

More details about CrayCro can be found on the

meeting website at http://craycro.biol.pmf.hr/. H

Ivana Maguire (president of Organising committee)

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CRAYFISH WORKSHOP: STATUS QUO OF

THE CRAYFISH ASTACUS ASTACUS IN LOWER SAXONY AND BREMEN, GERMANY

Fourteen representatives from fisheries, research and public authorities met on the 9th of November 2012 at the Institute for Marine Resources (IMARE) in Bremerhaven for a workshop focused on crayfish. The aim of the workshop was the determination of the status quo of the threatened crayfish Astacus astacus in Lower Saxony and Bremen including the responsible stakeholders and the discussion of necessary actions and of intersections leading to project ideas for the protection of A. astacus.

Lectures: Cancer incidence in Lower Saxony - Dr. Arzbach,

State Office for Consumer Protection and Food Safety.

Presentation of the project "Astra" - Mr. Seemann / Mr. Lorkowski, IMARE.

Presentation of the crayfish farm in Poggenhagen - Mr. Göckemeyer, crayfish farmer.

Visit of the Center for Aquaculture Research (ZAF; U. Seemann).

Scope of the fish disease regulation in relation to crustacean aquaculture - Dr. Kleingeld, State Office for Consumer Protection and Food Safety.

Genetic characterization of strain breeding - Ms. Schrimpf, University of Koblenz-Landau.

Crayfish farm Wendland - Mr. Meyer.

Coexistence of Pacifastacus leniusculus and Astacus astacus - Mr. Everding, crayfish farmer.

Crayfish in the country of Osnabrück - Mr. Herpin, Public Environmental Service Osnabrück.

Crayfish project in the country of Osnabrück - Mr. Rötker, Planning Office.

Crayfish farming Krebszucht am Schloß - Mr. Harms, crayfish farmer.

Field Trip: Visit of the lobster station (Alfred Wegener Institute for

Polar and Marine Research) at Helgoland, Germany. Through the lively exchange on the crayfish workshop 2012 and the suggestion of Fam. Göckemyer, 14 participants met in Cuxhaven on 13 May 2013 to visit the lobster station on Helgoland. Besides visiting the station and very interesting tour including lectures by Dr. Schmalenbach and Prof. Dr. Buchholz about Homarus gammarus, an exchange of ideas was possible afterwards. The trip was completed with a walk around the island, taking the ferry back to Cuxhaven in the

late afternoon. H

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Short Articles

Figure 1. Group photo of the participants at the Helgoland

crayfish workshop.

Figure 2. Female lobster with eggs.

Figure 3. Male and female lobsters.

Crayfish News Volume 35 Issue 3-4: Page 6

These are a few photos (Photos 1 – 4) from a survey of 50

streams I did this summer. H

William R. Budnick Louisiana State University Ag-Center

Department of Renewable Natural Resources Office 128 Renewable Natural Resources Building

Email: wbudni1@lsu.edu

AUSTRALIAN ENDANGERED SPECIES: VICTORIAN BURROWING CRAYFISH

Burrowing crayfish are a particular challenge to survey and to conserve because they live underground, and their ability to disperse is extremely limited. Sometimes this means that impacts on their habitat go unnoticed and once affected, their populations are unlikely to recover quickly.

This series has discussed these challenges before when discussing burrowing crayfish in Western Australia and in Tasmania. The two Victorian species of burrowing crayfish that are critically endangered are the Warragul Burrowing Crayfish (Engaeus sternalis) and the Mallacoota Burrowing Crayfish (Engaeus mallacoota).

Both of these species are found in Gippsland, but one of them is a dark, glossy creature that prefers sandy soil in a remote bay near Mallacoota, while the other is a ghostly white or pale blue fuzzy specimen found in clay riverbanks in farmland near Warragul.

The site best known for the Warragul Burrowing Crayfish has been identified as Australia’s most diverse crayfish habitat because it supports four different burrowing crayfish species and at least two (possibly three) different species of spiny crayfish (Morey and Hollis 1997). I have visited this site on Labertouche Creek and it is one of the most unassuming biodiversity hotspots imaginable: a paddock that looks like any other in this part of Gippsland except for the fence that keeps the cows away from part of the river bank.

Part of the charm of burrowing crayfish is their cryptic

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Photo 2. Male, form I Painted Devil Crayfish, Cambarus (Lacuincambarus) ludovicianus Cambaridae. Taken by minnow trap baited with tilapia filet from Cypress Bayou, 5 miles west of Sieper, LA. Photo Credit: William Budnick 2013.

Photo 1. Burrow chimney of the Painted Devil Crayfish, Cambarus (Lacunicambarus) ludovicianus, in a road side ditch 5 miles east of Gillis, LA. Photo Credit: William Budnick 2013.

Photo 3. Juveniles of the rare and possibly endangered Calcasieu Painted Crayfish, Orconectes hathawayi blacki, captured from Hickory Branch, 10 miles East of Dequincy, Louisiana. Photo Credit: William Budnick 2013

Photo 4. Male, Form I Calcasieu Creek Crayfish, Procambarus (Pennides) pentastylus Cambaridae. Taken with electro-shocker from Ten Mile Creek, 1 mile south of Pitkin, LA. Photo Credit: William Budnick 2013.

Crayfish News Volume 35 Issue 3-4: Page 7

habit — because they spend most of their time underground, most people are unaware that they are even there. Usually, Engaeus crayfish leave muddy chimneys at the entrance to their burrows which allow the careful observer to register their presence. Unfortunately, the Warragul Burrowing Crayfish often build tunnels that do not connect to the surface. This means that finding them may mean damaging their habitat by digging up a section of the river bank.

Status

The Mallacoota Burrowing Crayfish (Engaeus mallacoota) is critically endangered and is officially known from a single location in the Croajingolong National Park. However, in the last two years surveys funded by the Bushfire Royal commission have expanded the range of the species slightly. Tarmo Raadik of Arthur Rylah Institute said the number of burrows indicate that the crayfish may be locally abundant, but their limited distribution still creates a risk for the long term.

Unfortunately, the sandy shifting soils they prefer and the deep burrows they dig make these animals very difficult to collect. Counting burrows is not sufficient evidence as other (non-endangered) burrowing species overlap with their distribution, so we need better ways of collecting these little diggers.

The Warragul Burrowing Crayfish (Engaeus sternalis) is also critically endangered and until recently was known from one location on Labertouche Creek. However, the Baw Baw Shire Council did a biodiversity assessment two years ago which expanded the range of the species. Warragul Burrowing Crays have been found in the townships of Warragul and Drouin by biologist Beverly Van Praagh. She also discovered that their burrows do come to the surface and have a small chimney, but only at certain times of year.

In general, for both species, we know virtually nothing about their ecology, population dynamics or habitat requirements.

Threats

Burrowing crayfish are particularly vulnerable to local environmental disturbance. Fire, drought or large sediment pulses can drastically affect populations, especially when their distribution is as limited as in these two cases.

Even though the Mallacoota Burrowing Crayfish is found in a national park, its range includes grazing land which means their burrows can be trampled by cattle. Timber harvesting in the adjacent state forest can impact vegetation and water quality in the streams that support these crayfish. Recreational fishing is still allowed in the national park, which can pose a risk if fishers mistake these crayfish as “yabbies” and use them as bait.

The Warragul Burrowing Crayfish has a different set of issues as its environment has been subject to 100 years of grazing which has caused streamside erosion and a loss of native vegetation. Gold mining may have had a large impact, and the introduction of trout creates the threat of predation if they venture into the creeks. Given its proximity to town, it is likely that its habitat has been also destroyed by the development of infrastructure such as roads.

Strategy

There are action plans in place for both the Mallacoota Burrowing Crayfish and the Warragul Burrowing Crayfish. Both plans call for more research and surveys due to the lack of information about these species.

One of the challenges is how to conduct surveys when traditional methods (digging up burrows) are destructive and time consuming. Pitfall traps have been used to collect Warragul Burrowing Crayfish in the past. More recently, the Arthur Rylah Institute had a 10% capture rate of Mallacoota Burrowing Crayfish using specially designed burrow tube traps. In future it may be possible to use eDNA (environmental DNA) to determine what species is in a burrow just by sampling the mud at the entrance.

Education is essential for the conservation of burrowing crayfish, because we can’t take appropriate actions unless we know that these gorgeous little creatures are digging in the soil under our feet.

The community education program for the Warragul Burrowing Crayfish began in 1995 with a brochure and some fencing to protect their habitat. It has recently been expanded significantly with information signs near a giant burrowing crayfish installed on the Two Towns Trail between Warragul and Drouin. According to Greg Hollis of the Baw

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A ghost of a crayfish: the Warragul burrowing crayfish. Photo Credit: Beverly Van Pragh

Mallacoota burrowing crayfish. Photo Credit: Jason Coughran.

Crayfish News Volume 35 Issue 3-4: Page 8

Baw Shire Council, further plans are underway for protecting crayfish habitat.

Conclusion

We have already come a long way in recognising and implementing conservation plans for burrowing crayfish. When I dug up my first terrestrial crayfish 20 years ago very few people knew about these engaging crustaceans. My experience in sharing information about burrowing crayfish is always positive: everyone who is lucky enough to meet one of these little guys is charmed by them.

With careful management of our rivers and wetlands we should be able to maintain these populations into the future. Their cryptic habits have a benefit – they can usually avoid predation and survive all but the most severe floods and fires without significant intervention. But we still need to manage their habitats, protect native vegetation and keep the creeks and rivers clean.

And if you get a chance, visit the Two Towns Walk in Warragul and Drouin and keep your eye out for small holes in the mud, knowing that they may lead to the elaborately branched underground world of the burrowing crayfish, a world about which we know virtually nothing.

Susan Lawler Department of Environmental

Management and Ecology La Trobe University

First published on The Conversation on 7 November 2013, as part of their series on Australian endangered species.

CRAYFISH PLAGUE AGENT DETECTED IN THE ‘NEW’ INVASIVE NORTH AMERICAN

CRAYFISH ORCONECTES IMMUNIS IN GERMANY

Although all North American crayfish species are suspected to be carriers of the crayfish plague pathogen Aphanomyces astaci (OIE 2009), only three of the North American crayfish species present in the wild in Europe have been shown to be a carrier of the pathogen so far: the signal crayfish (Pacifastacus leniusculus) (Unestam and Weiss 1970), the spiny-cheek crayfish (Orconectes limosus) (Vey et al. 1983), and the red swamp crayfish (Procambarus clarkii) (Diéguez-Uribeondo and Söderhäll 1993). These species belong to the “Old” non-indigenous crayfish species in Europe, i.e. have been introduced into European waters before 1975 (summarized by Holdich et al. 2009). One “New” non-indigenous crayfish species in Europe is the North American calico crayfish (Orconectes immunis). Calico crayfish have recently invaded the Upper Rhine plain, where they seem to displace their invasive predecessor spiny-cheek crayfish (Gelmar et al. 2006; Chucholl 2012).

In the present study, we examined whether calico crayfish also carries A. astaci. We collected 50 calico crayfish (Figure 1) and 10 spiny-cheek crayfish from the River Rhine close to the currently known downstream invasion front of calico crayfish (Germersheim site) and 32 calico crayfish from a channel near the site of original introduction (Bühl site). We evaluated the A. astaci carrier status by applying the TaqMan® minor groove binder (MGB) real-time PCR (qPCR) according to Vrålstad et al. (2009).

At the sampling site Germersheim, we detected A. astaci in 23 out of 50 calico crayfish (46%) and in 6 out of 10 spiny-cheek crayfish (60%). Furthermore, A. astaci was detected in 26 of the 32 tested calico crayfish from the Bühl site (81%).

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Figure 1. A male calico crayfish from site Germersheim. Note the dense hair tufts (arrows) on the chelae that distinguish O. immunis from other crayfish species currently present in the wild in Europe.

Some towns have giant bananas, others have giant burrowing

crayfish. Photo Credit: Greg Hollis.

Crayfish News Volume 35 Issue 3-4: Page 9

The positive verification of calico crayfish as a carrier of A. astaci adds another species to the list of highly dangerous non-indigenous species. Furthermore, we confirmed the infection in spiny-cheek crayfish co-existing with calico crayfish in the River Rhine. The positive carrier status of calico crayfish is worrying, given the fast and successful spread of this “New” non-indigenous crayfish species in the Upper Rhine plain. Preliminary field observations suggest that calico crayfish inhabit a wider spectrum of habitats than spiny-cheek crayfish (Chucholl 2012) and might be able to spread into areas unoccupied by spiny-cheek crayfish.

It is important to note that different North American crayfish species seem to carry different strains of A. astaci (Huang et al. 1994; Kozubíková et al. 2011) and that these strains can vary in their virulence (Jussila et al. 2011; Viljamaa-Dirks et al. 2011). Further research needs to answer whether calico crayfish are carriers of a different strain of A. astaci, possibly new to Europe. It is yet unclear whether calico crayfish were already carriers of A. astaci when they were introduced to the Rhine catchment or whether they were initially uninfected and got infected later, when they came into contact with A. astaci-carrying spiny-cheek crayfish. The positive verification of the crayfish plague pathogen in the single species population at the Bühl site supports the hypothesis that calico crayfish were already infected when they were introduced to Europe, yet does not rule out the possibility of a gradual upstream infection from A. astaci-carrying spiny-cheek crayfish in the Rhine River.

The identification of calico crayfish as the fourth invasive crayfish species to be a carrier of A. astaci in Europe supports the concern that all North American crayfish species in European waters are carriers of the crayfish plague pathogen. Such knowledge is crucial for conservation of native crayfish stocks and risk assessment of non-indigenous

species introductions. H

Anne Schrimpf*1, Christoph Chucholl2 Thomas Schmidt1 and Ralf Schulz1

1University Koblenz-Landau, Institute for Environmental Sciences, Fortstrasse 7, 76829 Landau, Germany

2 Fisheries Research StationBW, Argenweg 50/1, 88085 Langenargen, Germany

*E-mail: schrimpf@uni-landau.de

Literature Cited

Chucholl C (2012). Understanding invasion success: life-history traits and feeding habits of the alien crayfish Orconectes immunis (Decapoda, Astacida, Cambaridae). Knowledge and Management of Aquatic Ecosystems 404, 04.

Diéguez-Uribeondo J and Söderhäll K (1993). Procambarus clarkii Girard as a vector for the crayfish plague fungus, Aphanomyces astaci Schikora. Aquaculture and Fisheries Management 24:761–765.

Gelmar C, Pätzold F, Grabow K and Martens A (2006). Der Kalikokrebs Orconectes immunis am nördlichen Oberrhein: ein neuer amerikanischer Flusskrebs breitet sich rasch in Mitteleuropa aus (Crustacea: Cambaridae). Lauterbornia 56:15–25.

Holdich DM, Reynolds JD, Souty-Grosset C and Sibley PJ (2009). A review of the ever increasing threat to European crayfish from non-indigenous crayfish species. Knowledge and Management of Aquatic Ecosystems 11:394–395.

Huang TS, Cerenius L and Söderhäll K (1994). Analysis of genetic diversity in the crayfish plague fungus, Aphanomyces astaci, by random amplification of polymorphic DNA. Aquaculture 126:1–9.

Jussila, J, Makkonen J, Vainikka A, Kortet R, Kokko H (2011). Latent crayfish plague (Aphanomyces astaci) infection in a robust wild noble crayfish (Astacus astacus) population. Aquaculture 321:17–20.

Kozubíková E, Viljamaa-Dirks S, Heinikainen S, Petrusek A (2011). Spiny-cheek crayfish Orconectes limosus carry a novel genotype of the crayfish plague agent Aphanomyces astaci. Journal of Invertebrate Pathology 108:214–216.

OIE (2009). Crayfish plague (Aphanomyces astaci). Manual of Diagnostic Tests for Aquatic animals. Chapter 2.2.1. http://www.oie.int/fileadmin/Home/eng/Health_standards/aahm/2010/2.2.01_CRAYFISH.pdf (Accessed 15 October 2012)

Unestam T, Weiss DW (1970). Host-parasite relationship between freshwater crayfish and the crayfish disease fungus, Aphanomyces astaci. Responses to infection by a susceptible and a resistant species. Journal of General Microbiology 60:77–90.

Vey A, Söderhäll K, Ajaxon R (1983). Susceptibility of Orconectes limosis Raff. to the crayfish plague, Aphanomyces astaci Schikora. Freshwater Crayfish 5:284–291.

Viljamaa-Dirks S, Heinikainen S, Nieminen M, Vennerström P, Pelkonen S (2011). Persistent infection by crayfish plague Aphanomyces astaci in a noble crayfish population – a case report. Bulletin of the European Association of Fish Pathologists 31(5):182–188.

Vrålstad T, Knutsen AK, Tengs T, Holst-Jensen A (2009). A quantitative TaqMan® MGB real-time polymerase chain reaction based assay for detection of the causative agent of crayfish plague Aphanomyces astaci. Veterinary Microbiology 137:146–155.

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Crayfish News Volume 35 Issue 3-4: Page 10

demands on himself were equally strong. Not only should results be perfect, but the language of an article or presentation had to be absolutely correct. As a result, Per’s students ended up in high positions at major universities all over Scandinavia, with several being hired as Professors of Ecology.

One of Per’s students was Sture Abrahamsson, who conducted his thesis research on crayfish. Per followed and guided Sture’s work until Sture died tragically in 1973, but even thereafter, he oversaw a project attempting to restore Swedish crayfish waters. Per Brinck was the first President of the International Association of Astacology (IAA) from 1972 – 1974. He took part in most of the early symposia, and he was chairman of the organizing committee for the symposium held in Lund in 1984. He was also Chairman of the Sture Abrahamsson Memorial Foundation.

In 1974, Per Brinck was appointed a member of the Swedish Academy of Sciences, and in 1987, his final triumph came when a new Ecology building was built in Lund. He is considered the father of ecology in Scandinavia, and he is missed by the whole academic community.

A Swedish poet, E. A. Karlfeldt, once wrote back in 1898 in a famous poem about Fridolin, his own alter ego, that he...

“…speaks with peasants in peasant fashion,

and with learned men in latin”

Such a man was Per Brinck. H

Stellan Karlsson Smedstorp, Sweden

eva.stellan@sydskane.nu

Francesca Gherardi Memorial Prize 2014

The Francesca Gherardi Memorial Prize of 5,000 Euro is awarded annually by the Department of Biology, University of Florence, Italy. The prize will be donated by the Gherardi family in memory of Prof. Francesca Gherardi, who made remarkable contributions to the fields of Crustacean Behaviour and Invasion Biology. The prize recognizes and commemorates the fine example that Prof. Francesca Gherardi’s own life set for young scientists.

Applications for the 2014 prize for outstanding research in the field of Crustacean Behaviour are open to researchers under 40, of all nationalities, lacking permanent positions.

The closing date for submissions is 15 January 2014.

Submissions in electronic format only are to be sent to:

Prof. Felicita Scapini Department of Biology, University of Florence,

Via Romana 17, 50125 Italy felicita.scapini@unifi.it

Please refer to the Application Guidelines for details (see below).

Application Guidelines

Applications should be made online with supporting documents sent to Prof. Scapini. The application [in English] should include a CV, three articles published in peer-reviewed journals, and a letter attesting that the applicant holds no permanent research or teaching position.

The selected researcher (prize winner) will receive the award at the Department of Biology, University of Florence on occasion of a memorial event in honor of Francesca Gherardi in spring 2014. The awarded researcher will present his/her research on that occasion.

The Award Committee is headed by Prof. Felicita Scapini, assisted by prof. Bella Galil and Prof. Marco Vannini and the zoologists of the Department of Biology University of Florence.

See http://www.bio.unifi.it/art-129-francesca-gherardi-prize-2014.html for additional details. H

(Continued from page 3)

Welcome Message from the IAA20 Chair

Tadashi Kawai I am honoured and delighted to welcome you to

IAA20. The information supplied below should help you in planning ahead to attend the meeting. Additional de-tails will be provided on the IAA20 website in January 2014. Symposium Dates: 22(Mon.)-26(Fri.) September 2014. Venue: Maruyama Zoo, Sapporo, Japan (http://

www.city.sapporo.jp/zoo/). Registration Fee: Approximately 10,000-15,000 Japa-

nese Yen (= US $100-$150). Joint Pre-meeting: IAA & CSJ (Carcinological Society of

Japan, http://rose.hucc.hokudai.ac.jp/~s16828/cr/e-site/Top_page.html) Joint International Conference on Crustacea, which will be held at Sapporo, Japan on 20-21 September, 2014. H

EDITOR’S NOTE: This article will also appear in Freshwater Crayfish v19(2) as an “In Memorium” piece along with a full

listing of Per Brinck’s publications over the years.

Crayfish News Volume 35 Issue 3-4: Page 11

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Crayfish News Volume 35 Issue 3-4: Page 12

(2013). Effects of natural flooding and manual trapping on the facilitation of invasive crayfish-native amphibian coex-istence in a semi-arid perennial stream. Journal of Arid En-vironments 98:109–112.

Klobu, Ar GIV, Podnar M, Jeli, I. M, El, Franjevi, Damjan, Faller M, Tambuk A, Gottstein S, Simi, Vladica and Maguire I (2013). Role of the Dinaric Karst (western Bal-kans) in shaping the phylogeographic structure of the threatened crayfish Austropotamobius torrentium. Fresh-water Biology 58(6):1089–1105.

Kozubíková-Balcarová E, Koukol O, Martín MP, Svoboda J, Petrusek A and Diéguez-Uribeondo J (2013). The diversity of oomycetes on crayfish: Morphological vs. molecular identification of cultures obtained while isolating the cray-fish plague pathogen. Fungal Biology 117(10):682–691.

Kung P-C, Wu S-H, Nagaraju GPC, Tsai W-S and Lee C-Y (2013). Crustacean hyperglycemic hormone precursor tran-scripts in the hemocytes of the crayfish Procambarus clarkii: Novel sequence characteristics relating to gene splicing pattern and transcript stability. General and Com-parative Endocrinology 186:80–84.

Lan J-F, Zhou J, Zhang X-W, Wang Z-H, Zhao X-F, Ren Q and Wang J-X (2013). Characterization of an immune deficiency homolog (IMD) in shrimp (Fenneropenaeus chinensis) and crayfish (Procambarus clarkii). Developmental and Compar-ative Immunology 41(4):608–617.

Leichter CA, Thompson N, Johnson BR and Scott JG (2013). The high potency of ME-5343 to aphids is due to a unique mechanism of action. Pesticide Biochemistry and Physiolo-gy 107(2):169–176.

Lin Y-C, Chen J-C, Chen Y-Y, Liu C-H, Cheng W, Hsu C-H and Tsui W-C (2013b). Characterization of white shrimp Li-topenaeus vannamei integrin β and its role in immunomod-ulation by dsRNA-mediated gene silencing. Developmental and Comparative Immunology 40(2):167–179.

Liu Y-T, Chang C-I, Hseu J-R, Liu K-F and Tsai J-M (2013). Im-mune responses of prophenoloxidase and cytosolic manga-nese superoxide dismutase in the freshwater crayfish Cherax quadricarinatus against a virus and bacterium. Mo-lecular Immunology 56(1–2):72–80.

Machida Y and Akiyama Y (2013). Impacts of invasive crayfish (Pacifastacus leniusculus) on endangered freshwater pearl mussels (Margaritifera laevis and M. togakushiensis) in Japan. Hydrobiologia 720(1):145–151.

Makkonen J, Jussila J, Koistinen L, Paaver T, Hurt M and Kokko H (2013a). Fusarium avenaceum causes burn spot disease syndrome in noble crayfish (Astacus astacus). Jour-nal of Invertebrate Pathology 113(2):184–190.

Martin AE, Hoover TM and Richardson JS (2013). Modeling the role of stage-structured agonistic interactions in onto-genetic habitat shifts. Behavioral Ecology 24(2):355–365.

Matallanas B, Ochando M, Alonso F and Callejas C (2013). Phylogeography of the white-clawed crayfish (Austropotamobius italicus) in Spain: Inferences from mi-crosatellite markers. Molecular Biology Reports 40(9):5327–5338.

McClain WR (2013). Effects of supplemental feeding on har-vest of red swamp crawfish, Procambarus clarkii, in for-age‐based Ponds. Journal of the World Aquaculture Society 44(4):593–599.

Meng Q, Chen J, Xu C, Huang Y, Wang Y, Wang T, Zhai X, Gu W and Wang W (2013). The characterization, expression and activity analysis of superoxide dismutases (SODs) from Procambarus clarkii. Aquaculture 406–407:131–140.

Molnár T, Vörös J, Szeder B, Takáts K, Kardos J, Katona G and Gráf L (2013). Comparison of complexes formed by a crustacean and a vertebrate trypsin with bovine pancreatic trypsin inhibitor – the key to achieving extreme stability? FEBS Journal 280(22):5750–5763.

Naddafi R and Rudstam LG (2013). Predator-induced behav-ioural defences in two competitive invasive species: The zebra mussel and the quagga mussel. Animal Behaviour 86(6):1275–1284.

Nguyen GV, Hanson TR and Jolly CM (2013). A demand anal-ysis for crustaceans at the U.S. retail store level. Aquacul-ture Economics and Management 17(3):212–227.

Noonin C, Watthanasurorot A, Winberg S and Söderhäll I (2013). Circadian regulation of melanization and prokineti-cin homologues is conserved in the brain of freshwater crayfish and zebrafish. Developmental and Comparative Immunology 40(2):218–226.

Ou J, Li Y, Ding Z, Xiu Y, Wu T, Du J, Li W, Zhu H, Ren Q, Gu W and Wang W (2013). Transcriptome-wide identification and characterization of the Procambarus clarkii microRNAs potentially related to immunity against Spiroplasma eri-ocheiris infection. Fish and Shellfish Immunology 35(2):607–617.

Peters J and Lodge D (2013). Habitat, predation, and coexist-ence between invasive and native crayfishes: Prioritizing lakes for invasion prevention. Biological Invasions 15(11):2489–2502.

Palaoro AV, Dalosto MM, Costa GC and Santos S (2013). Niche conservatism and the potential for the crayfish Pro-cambarus clarkii to invade South America. Freshwater Biol-ogy 58(7):1379–1391.

Qichen J, Linlan L, Guangzhen J, Ewan M, Qing W, Wenting H, Siming D and Jiaxin Y (2012). Acute effects of ammonia on antioxidative response and gill Na+/K+ ATPase activity of juvenile Australian red claw crayfish (Cherax quadricari-natus). Journal of Freshwater Ecology 27(4):551–560.

Ríos V, Moreno I, Prieto AI, Puerto M, Gutiérrez-Praena D, Soria-Díaz ME and Cameán AM (2013). Analysis of MC-LR and MC-RR in tissue from freshwater fish (Tinca tinca) and crayfish (Procambarus clarkii) in tench ponds (Cáceres,

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Crayfish News Volume 35 Issue 3-4: Page 13

Spain) by liquid chromatography–mass spectrometry (LC–MS). Food and Chemical Toxicology 57:170–178.

Rodriguez-Gil JL, San Sebastián Sauto J, González-Alonso S, Sánchez Sanchez P, Valcarcel Y and Catalá M (2013). De-velopment of cost-effective strategies for environmental monitoring of irrigated areas in Mediterranean regions: Traditional and new approaches in a changing world. Agri-culture, Ecosystems & Environment 181:41–49.

Rogowski DL, Sitko S and Bonar SA (2013). Optimising con-trol of invasive crayfish using life‐history information. Fresh-water Biology 58(6):1279–1291.

Romero A, Verwijlen T, Guerrero A and Vermant J (2013b). Interfacial properties of crayfish protein isolate/chitosan mixed films. Food Hydrocolloids 32(2):395–401.

Rusaini, La Fauce KA, Elliman J, Bowater RO and Owens L (2013). Endogenous Brevidensovirus-like elements in Cherax quadricarinatus: Friend or foe? Aquaculture 396–399:136–145.

Schrimpf A, Maiwald T, VrÅLstad T, Schulz HK, MietaNa P and Schulz R (2013). Absence of the crayfish plague patho-gen (Aphanomyces astaci) facilitates coexistence of Europe-an and American crayfish in central Europe. Freshwater Biology 58(6):1116–1125.

Shengli L, Shiyuan G, Jinmei L and Wenhu H (2013). Effects of water temperature, photoperiod, eyestalk ablation, and non-hormonal treatments on spawning of ovary-mature red swamp crayfish. North American Journal of Aquaculture 75(2):228–234.

Shulse CD, Semlitsch RD, Trauth KM and Cadotte M (2013). Mosquitofish dominate amphibian and invertebrate com-munity development in experimental wetlands. Journal of Applied Ecology 50(5):1244–1256.

Sousa R, Freitas FEP, Mota M, Nogueira AJA and Antunes C (2013). Invasive dynamics of the crayfish. Aquatic Conserva-tion: Marine and Freshwater Ecosystems 23(5):656–666.

Tierney AJ, Andrews K, Happer KR and White MKM (2013). Dear enemies and nasty neighbors in crayfish: Effects of social status and sex on responses to familiar and unfamiliar conspecifics. Behavioural Processes 99:47–51.

Tomina Y, Kibayashi A, Yoshii T and Takahata M (2013). Chronic electromyographic analysis of circadian locomotor activity in crayfish. Behavioural Brain Research 249:90–103.

Tropea C and López Greco LS (2013). Effect of long‐term in-jection of dopamine on the ovarian growth of Cherax quadricarinatus juvenile females (Parastacidae, Decapoda). Acta Zoologica 94(4):462–470.

Von Borstel FD, Suárez J, de la Rosa E and Gutiérrez J (2013). Feeding and water monitoring robot in aquaculture greenhouse. Industrial Robot: An International Journal 40(1):10–19.

Wang D-L, Sun T, Zuo D, Wang L-M, Wang Q and Zhao Y-L (2013a). Cloning and tissue expression of hemocyanin gene

in Cherax quadricarinatus during white spot syndrome virus infection. Aquaculture 410–411:216–224.

Wang L-m, Zuo D, Lv W-w, Wang D-l, Liu Aj and Zhao Y (2013b). Characterization of Cdc2 kinase in the red claw crayfish (Cherax quadricarinatus): Evidence for its role in regulating oogenesis. Gene 515(2):258–265.

Wang L, Zuo D, Lv W, Li J, Wang Q and Zhao Y (2013c). Effects of dietary soybean lecithin on gonadal development and vitellogenin mRNA expression in the female redclaw crayfish Cherax quadricarinatus (von Martens) at first matu-ration. Aquaculture Research 44(8):1167–1176.

Ward David L, Morton-Starner R and Hedwall Shaula J (2013). An evaluation of liquid ammonia (ammonium hy-droxide) as a candidate piscicide. North American Journal of Fisheries Management 33(2):400–405.

Westhoff JT and Sievert NA (2013). Mortality and growth of crayfish internally tagged with PIT tags. North American Journal of Fisheries Management 33(5):878–881.

White DT and Mumm JS (2013). The nitroreductase system of inducible targeted ablation facilitates cell-specific regenera-tive studies in zebrafish. Methods 62(3):232–240.

Wutz S and Geist J (2013). Sex- and size-specific migration patterns and habitat preferences of invasive signal crayfish (Pacifastacus leniusculus Dana). Limnologica - Ecology and Management of Inland Waters 43(2):59–66.

Xia X-F, Zheng J-J, Shao G-M, Wang J-L, Liu X-S and Wang Y-F (2013). Cloning and functional analysis of glutathione perox-idase gene in red swamp crayfish Procambarus clarkii. Fish and Shellfish Immunology 34(6):1587–1595.

Xiao-wen C, Wei-jian S, Jie Z, Jin Z, Yuan J, Zen-yun Z, Bin W, Ke-yao Y, Han L, Hong-zhen L and Chong-yu S (2013). A comparative study of the ionization modes in GC–MS multi-residue method for the determination of organochlorine pesticides and polychlorinated biphenyls in crayfish. Food Analytical Methods 6(2):445–456.

Yano T, Ibusuki S, Takasaki M and Tsuneyoshi I (2013). Dime-thylsulfoxide potentiates the nerve conduction–blocking effect of lidocaine without augmentation of the intracellular lidocaine concentration in the giant axon of crayfish in vitro. Fundamental and Clinical Pharmacology 27(4):402–408.

Zeng Y (2013). Procambarin: A glycine-rich peptide found in the haemocytes of red swamp crayfish Procambarus clarkii and its response to white spot syndrome virus challenge. Fish and Shellfish Immunology 35(2):407–412.

Zhang X-W, Liu Y-Y, Mu Y, Ren Q, Zhao X-F and Wang J-X (2013a). Overexpression of a C-type lectin enhances bacteri-al resistance in red swamp crayfish Procambarus clarkii. Fish and Shellfish Immunology 34(5):1112–1118.

Zhang X-W, Ren Q, Zhang H-W, Wang K-K and Wang J-X (2013b). A C-type lectin could selectively facilitate bacteria clearance in red swamp crayfish, Procambarus clarkii. Fish

and Shellfish Immunology 35(5):1387–1394. H

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Arredondo, Figueroa JL, Ponce, Palafox JT, Shirai, Matsu-moto K, Pérez, Zavaleta Á, Barriga, Sosa IDLÁ and Ruiz Luna A (2013). Effects of including shrimp protein hydroly-sate in practical diets on the growth and survival of redclaw crayfish hatchlings Cherax quadricarinatus (Von Martens, 1868). Aquaculture Research 44(6):966–973.

Baldridge AK and Lodge DM (2013). Intraguild predation be-tween spawning smallmouth bass (Micropterus dolomieu) and nest‐raiding crayfish (Orconectes rusticus): implications for bass nesting success. Freshwater Biology 58(11):2355–2365.

Belinda G, Aldridge David C and Chris F (2013). The ‘dirty dozen’: socio‐economic factors amplify the invasion poten-tial of 12 high‐risk aquatic invasive species in Great Britain and Ireland. Journal of Applied Ecology 50(3):757–766.

Bisen PS and Raghuvanshi R (2013). Paragonimiasis. Pp. 470–491. In: Emerging Epidemics: Management and Control. Bisen PS and Raghuvanshi R (eds.). John Wiley and Sons, Inc.

Brady S (2013). Incidental aquaculture in California's rice pad-dies: Red swamp crawfish. Geographical Review 103(3): 336–354.

Calvo NS, Stumpf L, Sacristán HJ and López Greco LS (2013). Energetic reserves and digestive enzyme activities in juve-niles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-return. Aquaculture 416–417:85–91.

Capinha C, Larson ER, Tricarico E, Olden JD and Gherardi F (2013). Effects of climate change, invasive species, and dis-ease on the distribution of native European crayfishes. Con-servation Biology 27(4):731–740.

Chen C-H, Lin C-T, Hsu W-L, Chang Y-C, Yeh S-R, Li L-J and Yao D-J (2013a). A flexible hydrophilic-modified graphene micro-probe for neural and cardiac recording. Nanomedicine: Nan-otechnology, Biology and Medicine 9(5):600–604.

Chen H-L, Cao M-J, Cai Q-F, Su W-J, Mao H-Y and Liu G-M (2013b). Purification and characterisation of sarcoplasmic calcium-binding protein, a novel allergen of red swamp crayfish (Procambarus clarkii). Food Chemistry 139(1–4):213–223.

Chen H-L, Mao H-Y, Cao M-J, Cai Q-F, Su W-J, Zhang Y-X and Liu G-M (2013c). Purification, physicochemical and immuno-logical characterization of arginine kinase, an allergen of crayfish (Procambarus clarkii). Food and Chemical Toxicolo-gy 62:475–484.

Christopher John D (2013). Chelidonius: The Swallow Stone. Folklore 124(1):81–103.

Chucholl C (2013). Feeding ecology and ecological impact of an alien ‘warm-water’ omnivore in cold lakes. Limnologica - Ecology and Management of Inland Waters 43(4):219–229.

Dong C and Wang J (2013). Immunostimulatory effects of dietary fructooligosaccharides on red swamp crayfish, Pro-cambarus clarkii (Girard). Aquaculture Research 44(9):1416–1424.

Dörr AJM, Abete MC, Prearo M, Pacini N, La Porta G, Natali M and Elia AC (2013). Effects of selenium supplemented diets on growth and condition indexes in juvenile red swamp crayfish, Procambarus clarkii. Environmental Toxicol-ogy and Pharmacology 36(2):484–492.

Dyer JJ, Brewer SK, Worthington TA and Bergey EA (2013). The influence of coarse‐scale environmental features on current and predicted future distributions of narrow‐range endemic crayfish populations. Freshwater Biology 58(6):1071–1088.

Fang D-A, Huang X-M, Zhang Z-Q, Xu D-P, Zhou Y-F, Zhang M-Y, Liu K, Duan J-R and Shi W-G (2013). Molecular cloning and expression analysis of chymotrypsin-like serine prote-ase from the redclaw crayfish (Cherax quadricarinatus): A possible role in the junior and adult innate immune sys-tems. Fish and Shellfish Immunology 34(6):1546–1552.

Fitzsimons JD, Brown SB, Brown LR, Verreault G, Tardif R, Drouillard KG, Rush SA and Lantry JR (2013). Impacts of Diet on Thiamine Status of Lake Ontario American Eels. Transactions of the American Fisheries Society 142(5):1358–1369.

Fuertes JB, Celada JD, Carral JM, Sáez-Royuela M and Gon-zález-Rodríguez Á (2013b). Replacement of fish meal with poultry by-product meal in practical diets for juvenile cray-fish (Pacifastacus leniusculus Dana, Astacidae) from the onset of exogenous feeding. Aquaculture 404–405:22–27.

Gherardi F, Coignet A, Souty, Grosset C, Spigoli D and Aqui-loni L (2013). Climate warming and the agonistic behaviour of invasive crayfishes in Europe. Freshwater Biology 58(9):1958–1967.

Gross R, Palm S, Kõiv K, Prestegaard T, Jussila J, Paaver T, Geist J, Kokko H, Karjalainen A and Edsman L (2013). Mi-crosatellite markers reveal clear geographic structuring among threatened noble crayfish (Astacus astacus) popula-tions in Northern and Central Europe. Conservation Ge-netics 14(4):809–821.

Guan Z, Bing, Shui Y, Zhou X, Xu Z, Hong, Zhao C, Yang, Song C, Meng, Liao X and Ru (2013). Participation of cal-modulin in ovarian maturation induced by eyestalk ablation in red swamp crayfish Procambarus clarkii. Aquaculture Research 44(10):1625–1631.

Hansen GJA, Hein CL, Roth BM, Jake VZM, Gaeta JW, Latzka AW and Carpenter SR (2013). Food web consequences of long-term invasive crayfish control. Canadian Journal of Fisheries and Aquatic Sciences 70(7):1109–1122.

Iryna K, Antonín K and Pavel K (2013). Real-time monitoring of water quality using fish and crayfish as bio-indicators: a review. Environmental Monitoring and Assessment 185(6):5043–5053.

Kats LB, Bucciarelli G, Vandergon TL, Honeycutt RL, Matti-asen E, Sanders A, Riley SPD, Kerby JL and Fisher RN

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Literature of Interest to Astacologists To view abstracts, etc., click on a reference to be taken to the journal

website (some references may not contain links).