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Quick Guide

U-PLEX®

Custom Biomarker (Mouse) Assays

Components

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Additional Materials

Reagent Preparation

Prepare Conjugated Capture and Detection Antibodies

Prepare the U-PLEX Plate

Step 1 Create the Individual U-PLEX-Coupled Antibody Solutions

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Step 2 Prepare the Multiplex Coating Solution

Step 3 Coat the U-PLEX Plate

Prepare Calibrator Standards

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Reagent Preparation

Reagent Preparation/Assay Protocol Dilute Samples

Prepare Detection Antibody Solution

Prepare 2X Read Buffer T

Step 1 Add Samples and Calibrators

Step 2 Wash and Add Detection Antibody Solution

Step 3 Wash and Read

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