Pseudomonas fi
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Scientific classification
Kingdom: Bacteria
Phylum: Proteobacteria
Class: Gamma Proteobacteria
Order: Pseudomonadales
Family: Pseudomonadaceae
Genus: Pseudomonas
Species: P. aeruginosa
Binomial name
Pseudomonas aeruginosa
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Pseudomonas Infections
Survive where most organisms cannot; e.g., “oil-eating” bacteria are Pseudomonas.
(Slime layer)
Opportunistic pathogen
Flagella
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Pseudomonas aeruginosa
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Structure
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1.Exotoxin A Similar in structure to Diptheria toxin Causes Dermatonecrosis in burn
wounds, corneal damage in ocular infections, and tissue damage in chronic pulmonary infections.
Also this toxin is immunosuppressive
Heat labile hemolysinBreaks down lipids and lecithin causing tissue destructionStimulates inflammatory response
2.Phospholipase C
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3.Elastase and Alkaline Protease
• Destruction of elastin-containing tissues (blood vessels, lung tissue, skin), collagen, immunoglobulins, and complement factors
• Can produce hemorrhagic lesions (ecthyma gangrenosum)
• Inactivation of interferon
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Clinical Disease� Pulmonary Infections
� Burn Wound Infections and other skin and soft tissue infections (life threatening)
� UTI’s (especially catheterized)
� External Otitis (malignant OE, swimmer’s ear)
� Eye Infections and corneal ulceration via contaminated contact lens cleaning fluids
� Pseudomonal Endocarditis
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� Grow on most laboratory media� Blood agar � MacConkey’s agar � Minimal nutritional reqts.;
Many organic compounds used as C and N sources, but only a few carbohydrates by oxidative metabolism
� tolerate broad temperature spectrum
� its ability to grow at 42° C.
Isolation and identification
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Motility of organism
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Colony characteristic:
Characteristic grape-like odor and blue-green pus & colon Lactose negative on MacConkey’s agar Some strains produce diffusible pigments: Pyocyanin (blue); Fluorescein (yellow); Pyorubin (red) Fluorescence is helpful in early identification
CharacteristicsCharacteristicsBiochemical test:inability to ferment lactose,oxidase & catalase positiveNitrate reduction negative
Catalase test
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