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Transcript of PROTEINE ALIMENTARI Francesco Bonomi DeFENS -UNIMIiaa.entecra.it/WS2015/17_20 Bonomi 2.pdf ·...
![Page 1: PROTEINE ALIMENTARI Francesco Bonomi DeFENS -UNIMIiaa.entecra.it/WS2015/17_20 Bonomi 2.pdf · Proteine native DENATURAZIONE E RETICOLAZIONE-Chemical - pH - solvent - interfaces -](https://reader030.fdocuments.us/reader030/viewer/2022021722/5c686ce809d3f25c6a8b6f54/html5/thumbnails/1.jpg)
PROTEINE ALIMENTARI
Francesco Bonomi
DeFENS - UNIMIDeFENS - UNIMI
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FOOD SYSTEMS: CHALLENGES
COMPLEXITY
Intrinsic (raw materials of biological origin)
From processing (novel interactions)
HETEROGENEITY
Intrinsic (more than one ingredient!)
From processing (components differently affected)
PHYSICAL STATEPHYSICAL STATE
From frozen to piping hot
Plurality of phases
COMPONENTS OUGHT TO BE PRESENT,
EACH WITH A PROPER STRUCTURE
The case of proteins
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FOOD PROTEINS: BEFORE
ABUNDANCE
Governed by genetics, epigenetics, and more…
STRUCTURE
Intrinsic (raw materials of biological origin)
From unseen/unexpected processing
FUNCTION
The good, the bad, the ugly: the case of enzymes
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FOOD PROTEINS: DURINGIRREVERSIBILE COMPOSITIONAL AND RELATIONAL
CHANGES
- CHEMICAL OR ENZYMATIC MODIFICATION
- glycation & glycosylation
- oxidations- oxidations
- proteolysis
- NOVEL PROTEIN-PROTEIN COVALENT INTERACTION
- disulfide bridges
- transamination
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FOOD PROTEINS: DURINGREVERSIBILE STRUCTURAL CHANGES INVOLVE
NON-COVALENT BONDS
TRANSIENT (REVERSIBLE) STRUCTURAL MODIFICATIONS
IRREVERSIBLE STRUCTURAL MODIFICATIONSIRREVERSIBLE STRUCTURAL MODIFICATIONS
Depending on the treatment nature and intensity,
and on the peculiar features of the system
native transiently modified
irreversibly unfolded
reversible irreversible
![Page 8: PROTEINE ALIMENTARI Francesco Bonomi DeFENS -UNIMIiaa.entecra.it/WS2015/17_20 Bonomi 2.pdf · Proteine native DENATURAZIONE E RETICOLAZIONE-Chemical - pH - solvent - interfaces -](https://reader030.fdocuments.us/reader030/viewer/2022021722/5c686ce809d3f25c6a8b6f54/html5/thumbnails/8.jpg)
frac
tion
of th
e to
tal 0.8
1.0
Native species
Treatments of low intensity do not produce enough intermediate
PROTEINS STRUCTURAL CHANGES
time, arbitrary units
0 2 4 6 8 10
frac
tion
of th
e to
tal
0.0
0.2
0.4
0.6
0.8 Native species
intermediate
End product
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frac
tion
of th
e to
tal 0.8
1.0 Native species
End
Treatments of high intensity do not allow accumulation of intermediates
PROTEINS STRUCTURAL CHANGES
time, arbitrary units
0 2 4 6 8 10
frac
tion
of th
e to
tal
0.0
0.2
0.4
0.6
0.8 End product
intermediate
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1.0
Only treatments of appropriate intensity allow significant accumulation of the intermediate
PROTEINS STRUCTURAL CHANGES
time, arbitrary units
0 2 4 6 8 10
frac
tion
of th
e to
tal
0.0
0.2
0.4
0.6
0.8
Native species End product
intermediate
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FOOD PROTEINS: CHALLENGES
Final products (food analyst/technologist)
much studied:
treatment “reporters”biological & sensory properties
Unfolding intermediates (the protein (bio)chemist)
poorly studied:
real “substrates”
dictate rate & extent of the various paths occurring during processing
relevant to process modeling & optimization
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FOOD PROTEINS: DURING
CLASSES OF DENATURANTS (LOSS OF STRUCTURE!)
- Physical
- temperature (not “heat”!)
- pressure
- radiations
- Chemical
- pH
- solvent
- interfaces
- salts
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FOOD PROTEINS: DURING
CLASSES OF DENATURANTS (LOSS OF STRUCTURE!)
- Physical
- temperature (not “heat”!)
- high pressure (> 2000 atm…)
- mechanical
- Chemical
- pH
- solvent
- interfaces
- salts
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FOOD PROTEINS: DURING
DENATURANTS AT WORK
- Physical
- temperature (not “heat”!)
- pressure
COLLAGEN AND GELATIN: A PSEUDO REVERSIBLE SYSTEM
- radiations
- Chemical
- pH
- solvent
- interfaces
- salts
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FOOD PROTEINS: DURING
DENATURANTS AT WORK
- Physical
- temperature (not “heat”!)
- pressure
COLLAGEN AND GELATIN: A PSEUDO REVERSIBLE SYSTEM
- radiations
- Chemical
- pH
- solvent
- interfaces
- salts
![Page 17: PROTEINE ALIMENTARI Francesco Bonomi DeFENS -UNIMIiaa.entecra.it/WS2015/17_20 Bonomi 2.pdf · Proteine native DENATURAZIONE E RETICOLAZIONE-Chemical - pH - solvent - interfaces -](https://reader030.fdocuments.us/reader030/viewer/2022021722/5c686ce809d3f25c6a8b6f54/html5/thumbnails/17.jpg)
FOOD PROTEINS: DURING
DENATURANTS AT WORK
- Physical
- temperature (not “heat”!)
- pressure
COLLAGEN AND GELATIN: A PSEUDO REVERSIBLE SYSTEM
- radiations
- Chemical
- pH
- solvent
- interfaces
- salts
![Page 18: PROTEINE ALIMENTARI Francesco Bonomi DeFENS -UNIMIiaa.entecra.it/WS2015/17_20 Bonomi 2.pdf · Proteine native DENATURAZIONE E RETICOLAZIONE-Chemical - pH - solvent - interfaces -](https://reader030.fdocuments.us/reader030/viewer/2022021722/5c686ce809d3f25c6a8b6f54/html5/thumbnails/18.jpg)
FOOD PROTEINS: DURING
CONTROLLED DENATURATION AT WORK
- Physical
- temperature (not “heat”!)
- high pressure
- mechanical- mechanical
- Chemical
- pH
- solvent
- interfaces
- salts
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FOOD PROTEINS: DURING
CONTROLLED DENATURATION AT WORK
- Physical
- temperature (not “heat”!)
- high pressure
- mechanical
- Chemical
- pH
- solvent
- interfaces
- salts
![Page 20: PROTEINE ALIMENTARI Francesco Bonomi DeFENS -UNIMIiaa.entecra.it/WS2015/17_20 Bonomi 2.pdf · Proteine native DENATURAZIONE E RETICOLAZIONE-Chemical - pH - solvent - interfaces -](https://reader030.fdocuments.us/reader030/viewer/2022021722/5c686ce809d3f25c6a8b6f54/html5/thumbnails/20.jpg)
FOOD PROTEINS: DURING
CONTROLLED DENATURATION AT WORK
- Physical
- temperature (not “heat”!)
- high pressure
- mechanical PASTAacqua
aggiunta impasto e denaturazione - mechanical
- Chemical
- pH
- solvent
- interfaces
- salts
PASTA
acqua rimossa per essiccazione
denaturazione proteine
impasto e formazione legami
cottura: assunzione di
acqua
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FOOD PROTEINS: DURING
CONTROLLED DENATURATION AT WORK
- Physical
- temperature (not “heat”!)
- high pressure
- mechanicalSH
S-S
Proteine native
DENATURAZIONE E RETICOLAZIONE
- mechanical
- Chemical
- pH
- solvent
- interfaces
- salts
S-S
SH
S-S
Reticolo interproteico
Legami disolfuro:
intraproteina
interproteina
Legami idrofobici
intraproteina
interproteina
![Page 22: PROTEINE ALIMENTARI Francesco Bonomi DeFENS -UNIMIiaa.entecra.it/WS2015/17_20 Bonomi 2.pdf · Proteine native DENATURAZIONE E RETICOLAZIONE-Chemical - pH - solvent - interfaces -](https://reader030.fdocuments.us/reader030/viewer/2022021722/5c686ce809d3f25c6a8b6f54/html5/thumbnails/22.jpg)
impasto e formazione
legami
cottura, passaggio di acqua da
proteine ad amidostaling, phase 1
PANE
cessione di acqua a proteine:
rammollimento
staling, phase 2
perdita complessiva di
acqua: raffermimento
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FOOD PROTEINS: DURING
CONTROLLED DENATURATION AT WORK
- Physical
- temperature (not “heat”!)
- high pressure
- mechanical
proteina nativa: in nero le regioni
idrofobiche, in chiaro le regioni polari
denaturazione interfacciale diretta
goccia di grasso(non in scala)
- mechanical
- Chemical
- pH
- solvent
- interfaces, oil
- salts
interfacciale diretta(con idrocarburi o
solventi)denaturazione
meccanica
interazione di superficie
emulsione di grasso
in acqua
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FOOD PROTEINS: DURING
CONTROLLED DENATURATION AT WORK
- Physical
- temperature (not “heat”!)
- high pressure
- mechanicalpunto nodale
lamella
- mechanical
- Chemical
- pH
- solvent
- interfaces, gas
- salts scorrimento dell'acqua verso i punti nodali
fuoriuscita di gasevaporazione
dell'acqua dalle lamelle
aria, anidride
carbonica
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FOOD PROTEINS: DURING
CONTROLLED DENATURATION AT WORK
- Physical
- temperature (not “heat”!)
- high pressure
- mechanical- mechanical
- Chemical
- pH
- solvent
- interfaces, solid
- salts
![Page 26: PROTEINE ALIMENTARI Francesco Bonomi DeFENS -UNIMIiaa.entecra.it/WS2015/17_20 Bonomi 2.pdf · Proteine native DENATURAZIONE E RETICOLAZIONE-Chemical - pH - solvent - interfaces -](https://reader030.fdocuments.us/reader030/viewer/2022021722/5c686ce809d3f25c6a8b6f54/html5/thumbnails/26.jpg)
FOOD PROTEINS: DURING
CONTROLLED DENATURATION AT WORK
- Physical
- temperature (not “heat”!)
- high pressure
- mechanical- mechanical
- Chemical
- pH
- solvent
- interfaces, solid
- salts
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FOOD PROTEINS: DURING
CONTROLLED DENATURATION AT WORK
- Physical
- temperature (not “heat”!)
- high pressure
- mechanical- mechanical
- Chemical
- pH
- solvent
- interfaces, solid
- salts
![Page 28: PROTEINE ALIMENTARI Francesco Bonomi DeFENS -UNIMIiaa.entecra.it/WS2015/17_20 Bonomi 2.pdf · Proteine native DENATURAZIONE E RETICOLAZIONE-Chemical - pH - solvent - interfaces -](https://reader030.fdocuments.us/reader030/viewer/2022021722/5c686ce809d3f25c6a8b6f54/html5/thumbnails/28.jpg)
FOOD PROTEINS: DURING
CONTROLLED DENATURATION AT WORK
- Physical
- temperature (not “heat”!)
- high pressure
- mechanical- mechanical
- Chemical
- pH
- solvent
- interfaces, solid
- salts
![Page 29: PROTEINE ALIMENTARI Francesco Bonomi DeFENS -UNIMIiaa.entecra.it/WS2015/17_20 Bonomi 2.pdf · Proteine native DENATURAZIONE E RETICOLAZIONE-Chemical - pH - solvent - interfaces -](https://reader030.fdocuments.us/reader030/viewer/2022021722/5c686ce809d3f25c6a8b6f54/html5/thumbnails/29.jpg)
Controlling the polymers formation
Covalent and non-covalent aggregation mechanisms for chaotrope-treated BLG
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From low tide…
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… to ebb tide
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OPTICAL SPECTROSCOPIES
Infrared spectroscopy
Circular dichroism
Structural features of food proteins
Circular dichroism
Intrinsic
Binding of ligands
Fluorescence
Intrinsic (reporter amino acids)
Extrinsic (binding of ligands/probes)
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“MAGNETIC” SPECTROSCOPIES
NMR
homonuclear
Structural features of food proteins
homonuclear
heteronuclear
relaxometry (and the like)
imaging
EPR
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