Phytoplankton Diversity in New York Lakes
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Transcript of Phytoplankton Diversity in New York Lakes
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Phytoplankton Diversity in New York Lakes
Ryan Butryn and Rick Naro
http://www.durr.demon.co.uk/volvox.html
http://www.turnerdesigns.com/t2/instruments/10au.html
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Study Sites
• Analysis of phytoplankton diversity and chlorophyll-a levels in New York lakes:– Arbutus Lake– Wolf Lake– Catlin Lake– Oneida Lake– Green Lake– Onondaga Lake– Skaneateles Lake
Wolf Lake
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Chlorophyll Study Site Procedures
• Collect water sample from defined lake layers– epilimnion– metalimnion– hypolimnion
• Green Lake sampling included– chemocline– monimolimnion
Arbutus Lake
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Chlorophyll Lab Procedure
• Turner Designs 10-AU Field Fluorometer– Measures the fluorescence of
chlorophyll-a– Highly sensitive– Suitable for extractive and in vivo
analysis
• Sample Processing– 100ml water sample filtered– Filter paper dissolved in 10ml acetone
http://www.turnerdesigns.com/t2/instruments/10au.html
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Chlorophyll-a Results
Ska
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ga 0
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Chlorophyll-a (μg/l)
Sampling Location
Skaneateles
Arbutus
Wolf
Green
Catlin
Oneida
Onondaga
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A Special Case: Green Lake
• Lake profile appears to be similar to other low productivity lakes
• Highest chlorophyll-a level is found in the chemocline
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Green Lake Chlorophyll-a Profile
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Epilimnion Metalimnion Hypolimnion Chemocline Monimolimnion
Sample Location
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Why is chlorophyll-a so high?
• We hypothesize that green photosynthetic bacteria should be present in solution and masked by the purple bacteria
• Further processing is required to confirm this.
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Phytoplankton Collection
• Van Dorn trap used to sample each lake layer
• Samples stabilized with preservative solution
• Identified and counted under microscope
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Phytoplankton DivisionsArbutus Lake
Cyanophyta Chlorophyta Chrysophyta
Green Lake
Chlorophyta Pyrrophyta CryptophytaEuglenophyta Cyanophyta
Catlin Lake
Diatom Chrysophyta Cyanophyta Chlorophyta
Skaneate les Lake
Cyanophyta Euglenophyta Chlorophyta
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Phytoplankton DivisionsOneida Lake
Diatom Cyanophyta Euglenophyta
Chlorophyta Chrysophyta Pyrrophyta
Onondaga Lake
Euglenophyta Chlorophyta Cryptophyta
Cyanopyta diatom Chrysophyta
Quadricoccus euryhalinicus
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Catlin Phytoplankton Genera
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Hypolimnion
Metalimnion
Epilimnion
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Oneida Phytoplankton Genera
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Hypolimnion
Metalimnion
Epilimnion
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Onondaga Phytoplankton Genera
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Summary of Observations
• Chlorophyll– Highest in epilimnion and hypolimnion in eutrophic
lakes, lower in metalimnion– Relatively constant density in oligotrophic lakes– Green Lake a special case
• Phytoplankton– Increasing eutrophication = higher phytoplankton
diversity– Cyanophytes tend to dominate eutrophic lakes– Chlorophytes dominate more oligotrophic lakes– More plankton diversity in epilimnion, more plankton
numbers in metalimnion and hypolimnion