Dr Arunima karkun

Bacteriophages are viruses that infect bacterial cells.

Infected cells are used as hosts to replicate the virus.

E. coli phages used due to ease of culture and quick regeneration

It is used for cloning foreign genes with many other applications

In this process proteins and peptides are fused to the Capsid(surface) of the phage

The combination of the phage and peptide is known as a Fusion Protein

Different sets of genes are inserted into the genomes of multiple phages

These separate phages will only display one protein, peptide, or antibody

Collections of these phages can comprise Libraries

These Libraries are exposed to selected targets and only some phages will interact with targets

Recombinant DNA technology to incorporate foreign cDNA of interest into viral DNA.

Spliced into gene for a coat protein so the protein will be displayed on outside of phage particles

Unbound phages are washed away leaving only those showing affinity for the receptors

Eluted phages showing specificity are used to infect new host cells for amplification.

Cycle repeated 2-3 times for stepwise selection of best binding sequence.

The analysis process will be done where phages can be propagated then characterized with DNA sequencing.

3 types of common phages used in phage display are the M13 and F1

Virus takes up a small amount of area Through using multiple Virions

polypeptide libraries can be created, and each phage displays a random peptide

Epitope mapping and mimicking Identification of new receptors & ligands Drug discovery Epitope discovery – new vaccines Creation of antibody libraries Organ targeting

Proteins Isolated through phage display will have a specific interactions with its target

Whether this target is a antigen, protein, or disease it allows a quick effective treatment for it