PBG 431 Plant Genetics Recitation Mendelian inheritance
18
PBG 431 Plant Genetics Recitation Mendelian inheritance
description
PBG 431 Plant Genetics Recitation Mendelian inheritance. Heart disease, high cholesterol, obesity. Eating barley can help! A principal active ingredient is Beta glucan , a complex carbohydrate found in soluble fiber - PowerPoint PPT Presentation
Transcript of PBG 431 Plant Genetics Recitation Mendelian inheritance
PBG 431 Plant Genetics Recitation
Mendelian inheritance
Heart disease, high cholesterol, obesity
• Eating barley can help!• A principal active ingredient is Beta glucan, a
complex carbohydrate found in soluble fiber• Barley has the potential to have higher levels of
beta glucan than any other grain
High beta glucan varieties would be good but….
• Breeding for beta glucan per se is made complicated by quantitative inheritance
• The recessive (waxy starch) allele at the GBSSI locus has a pleiotropic effect - it increases beta glucan
• GBSSI is cloned and therefore the DNA sequence differences between waxy (wx) and non-waxy (Wx) be used as a basis for marker assisted selection
What is the candidate gene for waxy?
What cause the mutation?
Result of the mutation
Glucose
Amylose Amylopectin
Starch
GBSSI
90% amylopectinvs. 75% wild type
Scoring alleles at the Waxy locus
non-waxywaxy
SNP data
Hull-less (naked) varieties could make grain processing
easier……..• Breeding for naked seed is not difficult -
if you are willing to wait for plants to mature
• Nud___ genotypes are hulled; nudnud are hull-less
• The Nud gene has not been cloned - yet!
Hulled and hulless (naked)
Wx and Nud are on the same chromosome
• But they are far enough apart to show independent assortment (no linkage)
Oregon Wolfe Barley Map187 classical loci
BCD14340DsT-667Act8A12RbgMD17MWG837B22scind00046ABC165C25
Bmac039928GBM100730BCD09836GBM104249Bmag021159BG36994064ABC16079
JS10C109
MWG706A119KFP170126
MWG2028150ABC261151KFP257B152WMC1E8160MWG912162ABG387Ascssr04163166
1H
ABG0580scind026221ABG00813scssr1022641scssr0775945GBM106647Pox50scssr0338162Hot168Ebmac0684scssr0223669
scssr0033472ABG35675GBM102377scsnp0334390vrs196Bmag0125102DsT-41105MWG503110GBM1062111KFP203112MWG882A115ABG072133Ebmc0415150cnx1152Zeo1161GBM1019173Aglu5Aglu4176
MWG720179GBM1012185MWG949Ascssr08447193
2H
GBM10740MWG798B6Dst-279BCD70612
alm37Bmac020941ABC32545DsT-6748scssr2569163ABG37765Bmag022573
ABG49998
scsnp00940123
ABG004147
scind02281160MWG883165
HVM62183ABC805191
ABC172202
3H
MWG6340MWG07720HVM4023CDO54229scsnp0164830CDO12231hvknox335ABC303scssr2056941
CDO79544DST-46scind03751scind04312a
50
Tef2scind0172852
GBM102060Bmag035361scind1045568scssr1407980ABG47281GBM105984KFP22193Ebmac070195MWG652B96GBM1048102Hsh113HVM67114KFP241.1117ABG601127
4H
scssr023060MWG6187ABC48312ABG61013
ABG39541scssr0250348NRG045A61scsnp0426062Ale63
ABC30291scind1699194scssr1533496scsnp06144100srh111
RSB001A135scsnp001771420SU-STS1148ABG003B153
Tef3184MWG877188
ABG496203E10757Ascsnp02109224
ABG391228JS10B229ABC622236DsT-33239MWG602A253scssr03906255
5H
Bmac03160
MWG602B36JS10A48GBM102155GBM106867BG29929770HVM3173rob75scssr02093Bmag000976
ABG47487ABG38897scsnp21226103MWG820107
GBM1008134scind04312b142GBM1022145Bmac0040153DsT-32B157DsT-28170DsT-74171MWG798AMWG514173
6H
ABG7040
GBSS-I16AW98258024CDO475MWG08931
ABG38033
scsnp0046072ABC25573ABC165D74scssr1586487GBM103091MWG808DAK64294
scsnp00703103MWG2031104nud108lks2124ABC1024128Bmag0120136DsT-30137WG380B138ABC310B149Ris44151ABC253169ABG461A179WG380ADsT-69184GBM1065191KFP255215ThA1218
7H
• Short awns might be preferable to long awns…
LKs2____ = long awn lks2lks2 = short awn
Nud and LKs2 are on the same chromosome
• They are close enough to show linkage
Oregon Wolfe Barley Map187 classical loci
BCD14340DsT-667Act8A12RbgMD17MWG837B22scind00046ABC165C25
Bmac039928GBM100730BCD09836GBM104249Bmag021159BG36994064ABC16079
JS10C109
MWG706A119KFP170126
MWG2028150ABC261151KFP257B152WMC1E8160MWG912162ABG387Ascssr04163166
1H
ABG0580scind026221ABG00813scssr1022641scssr0775945GBM106647Pox50scssr0338162Hot168Ebmac0684scssr0223669
scssr0033472ABG35675GBM102377scsnp0334390vrs196Bmag0125102DsT-41105MWG503110GBM1062111KFP203112MWG882A115ABG072133Ebmc0415150cnx1152Zeo1161GBM1019173Aglu5Aglu4176
MWG720179GBM1012185MWG949Ascssr08447193
2H
GBM10740MWG798B6Dst-279BCD70612
alm37Bmac020941ABC32545DsT-6748scssr2569163ABG37765Bmag022573
ABG49998
scsnp00940123
ABG004147
scind02281160MWG883165
HVM62183ABC805191
ABC172202
3H
MWG6340MWG07720HVM4023CDO54229scsnp0164830CDO12231hvknox335ABC303scssr2056941
CDO79544DST-46scind03751scind04312a
50
Tef2scind0172852
GBM102060Bmag035361scind1045568scssr1407980ABG47281GBM105984KFP22193Ebmac070195MWG652B96GBM1048102Hsh113HVM67114KFP241.1117ABG601127
4H
scssr023060MWG6187ABC48312ABG61013
ABG39541scssr0250348NRG045A61scsnp0426062Ale63
ABC30291scind1699194scssr1533496scsnp06144100srh111
RSB001A135scsnp001771420SU-STS1148ABG003B153
Tef3184MWG877188
ABG496203E10757Ascsnp02109224
ABG391228JS10B229ABC622236DsT-33239MWG602A253scssr03906255
5H
Bmac03160
MWG602B36JS10A48GBM102155GBM106867BG29929770HVM3173rob75scssr02093Bmag000976
ABG47487ABG38897scsnp21226103MWG820107
GBM1008134scind04312b142GBM1022145Bmac0040153DsT-32B157DsT-28170DsT-74171MWG798AMWG514173
6H
ABG7040
GBSS-I16AW98258024CDO475MWG08931
ABG38033
scsnp0046072ABC25573ABC165D74scssr1586487GBM103091MWG808DAK64294
scsnp00703103MWG2031104nud108lks2124ABC1024128Bmag0120136DsT-30137WG380B138ABC310B149Ris44151ABC253169ABG461A179WG380ADsT-69184GBM1065191KFP255215ThA1218
7H
Wx vs. wx and Nud vs. nud data are from a doubled haploid
population
The expected segregation ratio is……..
If it were a backcross?An F2?
Phenotype #Observed #Expected O - E (O - E)2/E
Total 82 82
Let’s get some practical experience in segregation
analysis!!!!
