Molecular virology of hepatitis C virus - Journal of General Virology
Paul Klapper Clinical Virology Manchester Royal Infirmary hepatitis C dried blood spot testing.
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Transcript of Paul Klapper Clinical Virology Manchester Royal Infirmary hepatitis C dried blood spot testing.
Paul Klapper
Clinical Virology
Manchester Royal Infirmary
hepatitis C dried blood spot testing
- eluate – automated test system
HCV antibody detection
HCV antibody confirmatory testing
- HCV RNA
- HCV genotype
dried blood spot
HCV antibody detection - sensitivity - 100% - specificity - 100%
HCV antibody confirmatory testing - sensitivity 100% - specificity 100%
how good is it?
HCV RNA - sensitivity 96% - specificity 100%
HCV genotype - sensitivity 96% - specificity 100%
dried blood spot
HCV antibody detection - sensitivity - 100% - specificity - 100%
HCV antibody confirmatory testing - sensitivity 100% - specificity 100%
how good is it?
HCV RNA - sensitivity 96% - specificity 100%
HCV genotype - sensitivity 96% - specificity 100%
[orasure 91.7%; 99.2%]
[orasure - not available – 33-88%]
dried blood spot
but that’s’ not all….
using same sample
HBsAg – confirmation and full markers(sensitivity 96%)
HBc Ab – (sensitivity 100%)HIV testing – HIV antibody/antigen and confirmation (2 additional assays)
(sensitivity 98%)syphilis – good for infectious syphilis (sensitivity 100%)
not so good (sensitivity 50%)for detection of syphilis at any time
turnaround
working toward 24 hr turnaround time – these samples a little longer (overnight elution)
Web – secure server for results access
dried blood spot