PAM Fluorometry: Measuring Photosynthesis Brian Maniaci.
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Transcript of PAM Fluorometry: Measuring Photosynthesis Brian Maniaci.
![Page 1: PAM Fluorometry: Measuring Photosynthesis Brian Maniaci.](https://reader030.fdocuments.us/reader030/viewer/2022012908/56649ca45503460f949650be/html5/thumbnails/1.jpg)
PAM Fluorometry: Measuring Photosynthesis
Brian Maniaci
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Photosynthesis• 6 CO2 + 12 H20 + light Energy C6H12O2 + 6 O2
+ 6 H20
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Phytoplankton
•Photosynthetic organism
•Chlorophyll A
•Euphotic Layer
•Less than 5 mm long
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Marine
Food Chain
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Energy
•Use of light energy▫Photosynthesis▫Heat▫Fluorescence
•Fluorescence –emission of light at a lower energy/higher wavelength
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Measuring Photosynthesis
•Reactants▫Radioactive Carbon-14
•Products▫Winkler’s Method-O2 Concentration
•PAM Fluorometer▫Fluorescence of emitted Light
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PAM Fluorometer
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PAM Fluorometer
•Pulse amplitude modulated fluorometer
•Chlorophyll fluorescence
•Ideal environment
•Sensitive and noninvasive
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PAM 101 Unit
•3 mW laser diode
•Photodiode detector
•Fresnel lens
•Distance 0.5- 1 meter
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Laser Diode
•Promotes fluorescence
•Dual lasers to generate homogenous spot
•Emits light at 638 nm.
•Return signal magnified through Fresnel Lens
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Quantum Yield
•Definition: ▫∆F = Fmax –Fmin of fluorescence▫Fm = Fmax of fluorescence
•∆F/Fm = Quantum Yield
•Quantum yield of effective photosynthetic electron transport (PS 1)
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Characteristics
•Extremely sensitive
•Measuring system
•Can measure a single cell, chloroplast or macroalgae
•Doesn’t change physiology of the organism
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Chlorophyll Fluorescence Mapping•Blue LED (470 nm) used for excitation
•Measured at 665 nm
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Conclusion
•Photosynthesis
•Organism
•Ideal Environment
•Comparison of scientific data
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Acknowledgements
•Dr. Moline
•Esteemed Colleagues
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References:• "Algal Growth." A. Encyclopedia of Life Sciences. 1
vols. New York: Nature Group, 2002.
• Grunwald, B, and M Kuhl. "A System for Imaging Variable Chlorophyll Fluorescence of Aquatic Phototrophs." Ophelia 58 (2004): 79-89. 28 Feb. 2008.
• Ounis, A, S Evian, S Tosti, and I Moya. "Adaption of PAM-Fluorometer for Remote Sensing of Chlorophyll Fluorescence." Photosynthesis Research 68 (2001): 113-120. 28 Feb. 2008.